Revista de la Facultad de Ciencias Agrarias
Not a member yet
621 research outputs found
Sort by
Cover crops in pear (Pyrus communis) orchards: effects on soil nematode assemblage
Full text linkLong-term vegetation cover can affect soil organic carbon content and carbon flow within the soil food web. Nematode trophic structure and soil properties were evaluated in pear rows (intra) maintained without weeds applying herbicide and in the inter-rows (between rows) covered with: Medicago+grasses (MG), fescue or spontaneous vegetation. Soil samples were taken at 0-20 and 20-40 cm depths from 2012 to 2014. Nematode assemblage was different in each inter-row and row, mainly in the topsoil. The inter-rows were reservoirs of omnivores-predators. The MG inter-row promoted the highest accumulation of organic carbon, total N and exchangeable K in the soil. The enrichment index was related to the quantity of dry matter produced by cover crops with the highest index observed in MG. Nematode biomass showed a positive correlation with intra and inter-rows soil organic carbon. Carbon flow through bacterivores prevailed in intra-rows, while bacterivores or herbivores canalized the inter-rows.
Highlights:
Each cover crop (Medicago+grasses, spontaneous vegetation and fescue) was associated with a different nematode assemblage, mainly in the topsoil.
Omnivores-predators were higher in the inter-row than its associated row.
Nematode Biomass showed a positive correlation with the content of SOC in the row and in the inter-row.
Soil energy channels revealed higher carbon flow within the soil food web under Medicago+grasses and spontaneous vegetation than under fescue.Long-term vegetation cover can affect soil organic carbon content and carbon flow within the soil food web. Nematode trophic structure and soil properties were evaluated in pear rows (intra) maintained without weeds applying herbicide and in the inter-rows (between rows) covered with: Medicago+grasses (MG), fescue or spontaneous vegetation. Soil samples were taken at 0-20 and 20-40 cm depths from 2012 to 2014. Nematode assemblage was different in each inter-row and row, mainly in the topsoil. The inter-rows were reservoirs of omnivores-predators. The MG inter-row promoted the highest accumulation of organic carbon, total N and exchangeable K in the soil. The enrichment index was related to the quantity of dry matter produced by cover crops with the highest index observed in MG. Nematode biomass showed a positive correlation with intra and inter-rows soil organic carbon. Carbon flow through bacterivores prevailed in intra-rows, while bacterivores or herbivores canalized the inter-rows.
Highlights:
Each cover crop (Medicago+grasses, spontaneous vegetation and fescue) was associated with a different nematode assemblage, mainly in the topsoil.
Omnivores-predators were higher in the inter-row than its associated row.
Nematode Biomass showed a positive correlation with the content of SOC in the row and in the inter-row.
Soil energy channels revealed higher carbon flow within the soil food web under Medicago+grasses and spontaneous vegetation than under fescue
Characterization of Argentine commercial bee pollen intended for human consumption
Full text linkBee pollen is consumed as a dietary supplement. Its quality parameters are regulated by the Argentine Food Code (AFCode). The present study characterized 10 commercial dehydrated samples from five Argentine provinces to provide information on hygienic quality and health safety. We assessed their microbiological quality, including potential mycotoxins. We also determined their botanical origin and moisture. Results showed that seven out of ten samples presented lower counts of culturable heterotrophic mesophilic bacteria than those allowed by the AFCode. In contrast, all samples showed higher filamentous fungi and yeast counts than the level approved by the AFCode. No fumonisin or deoxynivalenol was detected; we observed only aflatoxin B2 in one sample and ochratoxin A in two. The results of the botanical origin of samples showed that all samples had a predominant pollen type, except one. The microbiological quality of all samples agreed with that required by the AFCode, except filamentous fungi and yeasts. In addition, we found variations among samples from the same province. Therefore, if pollen is intended for human consumption, appropriate hygiene standards must be applied to all bee pollen production operations.
Highlights:
The microbiological quality of bee pollen samples, regardless of the province or botanical origin, agreed with the requirements of the AFCode, except for filamentous fungus and yeast.
All samples showed filamentous fungus and yeast counts higher than the permitted level in the AFCode.
Mycotoxin levels found in bee pollen were far from the limits allowed in humans (10 µg.kg-1) by regulations for other foods.
All samples analyzed were pollen mixes verified by identifying numerous botanical species.
The characterization of Argentinian commercial bee pollen showed that it was suitable for human consumption.Bee pollen is consumed as a dietary supplement. Its quality parameters are regulated by the Argentine Food Code (AFCode). The present study characterized 10 commercial dehydrated samples from five Argentine provinces to provide information on hygienic quality and health safety. We assessed their microbiological quality, including potential mycotoxins. We also determined their botanical origin and moisture. Results showed that seven out of ten samples presented lower counts of culturable heterotrophic mesophilic bacteria than those allowed by the AFCode. In contrast, all samples showed higher filamentous fungi and yeast counts than the level approved by the AFCode. No fumonisin or deoxynivalenol was detected; we observed only aflatoxin B2 in one sample and ochratoxin A in two. The results of the botanical origin of samples showed that all samples had a predominant pollen type, except one. The microbiological quality of all samples agreed with that required by the AFCode, except filamentous fungi and yeasts. In addition, we found variations among samples from the same province. Therefore, if pollen is intended for human consumption, appropriate hygiene standards must be applied to all bee pollen production operations.
Highlights:
The microbiological quality of bee pollen samples, regardless of the province or botanical origin, agreed with the requirements of the AFCode, except for filamentous fungus and yeast.
All samples showed filamentous fungus and yeast counts higher than the permitted level in the AFCode.
Mycotoxin levels found in bee pollen were far from the limits allowed in humans (10 µg.kg-1) by regulations for other foods.
All samples analyzed were pollen mixes verified by identifying numerous botanical species.
The characterization of Argentinian commercial bee pollen showed that it was suitable for human consumption
Apple (Malus domestica) and pear (Pyrus communis) yield prediction after tree image analysis
Full text linkYield forecasting depends on accurate tree fruit counts and mean size estimation. This information is generally obtained manually, requiring many hours of work. Artificial vision emerges as an interesting alternative to obtaining more information in less time. This study aimed to test and train YOLO pre-trained models based on neural networks for the detection and count of pears and apples on trees after image analysis; while also estimating fruit size. Images of trees were taken during the day and at night in apple and pear trees while fruits were manually counted. Trained models were evaluated according to recall, precision and F1score. The correlation between detected and counted fruits was calculated while fruit size estimation was made after drawing straight lines on each fruit and using reference elements. The precision, recall and F1score achieved by the models were up to 0.86, 0.83 and 0.84, respectively. Correlation coefficients between fruit sizes measured manually and by images were 0.73 for apples and 0.80 for pears. The proposed methodologies showed promising results, allowing forecasters to make less time consuming and accurate estimates compared to manual measurements.
Highlights:
The number of fruits in apple and pear trees, could be estimated from images with promising results.
The possibility of estimating the fruit numbers from images could reduce the time spent on this task, and above all, the costs. This allow growers to increase the number of trees sampled to make yield forecasts.Yield forecasting depends on accurate tree fruit counts and mean size estimation. This information is generally obtained manually, requiring many hours of work. Artificial vision emerges as an interesting alternative to obtaining more information in less time. This study aimed to test and train YOLO pre-trained models based on neural networks for the detection and count of pears and apples on trees after image analysis; while also estimating fruit size. Images of trees were taken during the day and at night in apple and pear trees while fruits were manually counted. Trained models were evaluated according to recall, precision and F1score. The correlation between detected and counted fruits was calculated while fruit size estimation was made after drawing straight lines on each fruit and using reference elements. The precision, recall and F1score achieved by the models were up to 0.86, 0.83 and 0.84, respectively. Correlation coefficients between fruit sizes measured manually and by images were 0.73 for apples and 0.80 for pears. The proposed methodologies showed promising results, allowing forecasters to make less time consuming and accurate estimates compared to manual measurements.
Highlights:
The number of fruits in apple and pear trees, could be estimated from images with promising results.
The possibility of estimating the fruit numbers from images could reduce the time spent on this task, and above all, the costs. This allow growers to increase the number of trees sampled to make yield forecasts
Growth and yield of Syagrus schizophylla (Mart.) Glass. in response to light gradients
Full text linkThis research studied growth and yield of Syagrus schizophylla, an extinction-endangered ornamental palm, grown under five light gradients. The treatments were: G1 - PAR=1234.10 μmol photons m-2 s-1, G2 - PAR=913.16 μmol photons m-2 s-1, G3 - PAR=666.34 μmol photons m-2 s-1, G4 - PAR=419.56 μmol photons m-2 s-1 and G5 - PAR=534.77 μmol photons m-2s-1. Before the experiment and at three, five and seven months of treatment, growth (plant height, collar diameter, number of leaves, petiole length, leaf length and width), gas exchange, chlorophyll a, and leaf green color intensity were assessed. The highest net photosynthetic rates were observed in plants under G2, G3 and G4. Values of maximum quantum efficiency (Fv/Fm) over 0.75 were observed under G2. At seven months, estimated SPAD values were 36 in G2 plants and 32 in G1 plants. According to the Dickson quality index (DQI), presented the highest growth and development rates. We conclude that G2 suits plants to be transplanted into the field, whereas G3 would be best for plants grown under shade environments.
Highlights:
Syagrus schizophylla is an extinction endangered ornamental palm.
One layer of Red Chromatinet® 50% should be recommended for the first year of growth of schizophylla plants to be later transplanted to the field.
Two Red Chromatinet® 50% layers would be best for plants meant to grow under shade environments.
The highest net photosynthetic rates were observed in plants under G2, G3 and G4.This research studied growth and yield of Syagrus schizophylla, an extinction-endangered ornamental palm, grown under five light gradients. The treatments were: G1 - PAR=1234.10 μmol photons m-2 s-1, G2 - PAR=913.16 μmol photons m-2 s-1, G3 - PAR=666.34 μmol photons m-2 s-1, G4 - PAR=419.56 μmol photons m-2 s-1 and G5 - PAR=534.77 μmol photons m-2s-1. Before the experiment and at three, five and seven months of treatment, growth (plant height, collar diameter, number of leaves, petiole length, leaf length and width), gas exchange, chlorophyll a, and leaf green color intensity were assessed. The highest net photosynthetic rates were observed in plants under G2, G3 and G4. Values of maximum quantum efficiency (Fv/Fm) over 0.75 were observed under G2. At seven months, estimated SPAD values were 36 in G2 plants and 32 in G1 plants. According to the Dickson quality index (DQI), presented the highest growth and development rates. We conclude that G2 suits plants to be transplanted into the field, whereas G3 would be best for plants grown under shade environments.
Highlights:
Syagrus schizophylla is an extinction endangered ornamental palm.
One layer of Red Chromatinet® 50% should be recommended for the first year of growth of schizophylla plants to be later transplanted to the field.
Two Red Chromatinet® 50% layers would be best for plants meant to grow under shade environments.
The highest net photosynthetic rates were observed in plants under G2, G3 and G4
Biometric genetics in cowpea beans (Vigna unguiculata (L.) Walp) I: phenotypic and genotypic relations among production components
Full text linkIn the semi-arid region of Paraíba, cowpea has low productivity due to irregular rainfall and poor use of production technologies. An extensive study aimed at selecting more productive cultivars was conducted using biometric models. This first work had the following objectives: i. Quantify direct and indirect effects of primary and secondary components on grain production; ii. Identify variables with greater potential for cultivar selection in the semiarid region of Paraíba and iii. Determine the most appropriate selection strategies for the evaluated variables. The experiment was conducted in an experimental field. The influence of 6 primary and 6 secondary production components was evaluated on grain yield. Data were subjected to ANOVA. Genetic parameters, correlations and path analysis were estimated. Given the strong direct phenotypic and genotypic effects, pod yield results the most promising variable for higher grain yield selection. Direct and simultaneous selections are the most suitable strategies for the set of evaluated variables. However, further studies on selection indices are necessary to maximize genetic gains.
Highlights
Variables with greater potential were identified for the selection of superior cultivars of cowpea in the semiarid region of Paraíba.
The pod yield variable (PP) seems promising for cultivar selection considering higher grain yield (GY).In the semi-arid region of Paraíba, cowpea has low productivity due to irregular rainfall and poor use of production technologies. An extensive study aimed at selecting more productive cultivars was conducted using biometric models. This first work had the following objectives: i. Quantify direct and indirect effects of primary and secondary components on grain production; ii. Identify variables with greater potential for cultivar selection in the semiarid region of Paraíba and iii. Determine the most appropriate selection strategies for the evaluated variables. The experiment was conducted in an experimental field. The influence of 6 primary and 6 secondary production components was evaluated on grain yield. Data were subjected to ANOVA. Genetic parameters, correlations and path analysis were estimated. Given the strong direct phenotypic and genotypic effects, pod yield results the most promising variable for higher grain yield selection. Direct and simultaneous selections are the most suitable strategies for the set of evaluated variables. However, further studies on selection indices are necessary to maximize genetic gains.
Highlights
Variables with greater potential were identified for the selection of superior cultivars of cowpea in the semiarid region of Paraíba.
The pod yield variable (PP) seems promising for cultivar selection considering higher grain yield (GY)
Effect of cutting height, a bacterial inoculant and a fibrolytic enzyme on corn (Zea mays L.) silage quality
Full text linkThis study aimed to evaluate cutting height (CH) effects on ensiled corn without additives (C), with a lactic acid bacteria inoculant (L), a fibrolytic enzyme (F), or a mixture of both (FL), considering chemical composition and both in vitro digestibility dry matter (IVDMD) and in vitro neutral detergent fiber (IVNDFD). Corn was harvested at three different cutting heights (12, 25 or 42 cm above the soil) and ensiled with or without additives (AD). Data was analyzed according to a factorial design, with a 3 x 4 arrangement of treatments and three repeats. Dry matter content was highest in C12 and lowest in F12 (P<0.05) silages. As cutting height was higher, cell wall content was lower (P<0.05). Even considering it increased after the use of additives (P<0.05), the highest values occurred with FL silages. Crude protein was equal (P˃0.05) between CH and increased (P<0.05) with AD. The highest IVDMD was observed for 42 cm CH, while IVDMD and IVNDFD were higher in C and F, but lower with FL. None of the inoculation treatments, alone or combined improved corn silage quality. In fact, FL combination decreased such quality.
Highlights:
The efficacy of lactic acid bacteria (LAB) and exogenous fibrolytic enzyme (EFE) is a funtion of the amount of cell walls and sugars present in the maize plant at harvest.
Adding LAB and EFE to maize forage harvested at different cutting heights did not improve silage quality, in fact, the combination of both additives decreased silage quality.
Further studies are needed to prove the efficacy of EFE and its combination with LAB on maize silage quality.This study aimed to evaluate cutting height (CH) effects on ensiled corn without additives (C), with a lactic acid bacteria inoculant (L), a fibrolytic enzyme (F), or a mixture of both (FL), considering chemical composition and both in vitro digestibility dry matter (IVDMD) and in vitro neutral detergent fiber (IVNDFD). Corn was harvested at three different cutting heights (12, 25 or 42 cm above the soil) and ensiled with or without additives (AD). Data was analyzed according to a factorial design, with a 3 x 4 arrangement of treatments and three repeats. Dry matter content was highest in C12 and lowest in F12 (P<0.05) silages. As cutting height was higher, cell wall content was lower (P<0.05). Even considering it increased after the use of additives (P<0.05), the highest values occurred with FL silages. Crude protein was equal (P˃0.05) between CH and increased (P<0.05) with AD. The highest IVDMD was observed for 42 cm CH, while IVDMD and IVNDFD were higher in C and F, but lower with FL. None of the inoculation treatments, alone or combined improved corn silage quality. In fact, FL combination decreased such quality.
Highlights:
The efficacy of lactic acid bacteria (LAB) and exogenous fibrolytic enzyme (EFE) is a funtion of the amount of cell walls and sugars present in the maize plant at harvest.
Adding LAB and EFE to maize forage harvested at different cutting heights did not improve silage quality, in fact, the combination of both additives decreased silage quality.
Further studies are needed to prove the efficacy of EFE and its combination with LAB on maize silage quality.
Volunteer soybean (Glycine max) interference in bean (Phaseolus vulgaris) crops: ethoxysulfuron and halosulfuron critical level of damage and selectivity
Full text linkThis study aimed to determine the negative impact of volunteer soybean plants on bean crop yield and the tolerance of bean genotypes to the herbicides ethoxysulfuron and halosulfuron. To determine the impact of volunteer soybean plants on bean crops, a field experiment was developed, with sub-sub-plots, and four replications. The main plots contained two bean cultivars, while the sub-plots received two soybean sowing times (0 and 7 days after the beans had been sown), while the sub-sub plots contained five soybean plant densities (0, 5, 10, 20, and 40 plants m-2). The tolerance of the bean genotypes was evaluated with two experiments in a completely randomized design with three replications. They were arranged in a 28 x 3 factorial design (bean genotypes x herbicide doses). Each soybean plant per m2 reduced bean crop yield by 4%. The recommended doses of ethoxysulfuorn and halosulfuorn resulted in tolerance levels above 70% for all the studied bean genotypes.
Highlights:
The herbicides ethoxysulfuron and halosulfuron, are registered in Brazil for the control of volunteer soybean plants in bean crops.
To date, the impact generated by volunteer soybean plants competing with bean plants is unknown.
The tolerance of 28 bean cultivars to herbicides ethoxysulfuron and halosulfuron was determined using dose label and double the dose.
The impact of volunteer soybean plants on bean crops was determined using two bean cultivars and two soybean sowing times.
The bean genotypes displayed a highly variable response to the herbicides ethoxysulfuron and halosulfuron; however, when the label recommended dose of the herbicides was used, the tolerance levels observed were over 70%.
Each soybean plant is capable of causing a 4% reduction in bean plant grain yield, regardless of the establishment time of the soybean plants or the bean genotype.This study aimed to determine the negative impact of volunteer soybean plants on bean crop yield and the tolerance of bean genotypes to the herbicides ethoxysulfuron and halosulfuron. To determine the impact of volunteer soybean plants on bean crops, a field experiment was developed, with sub-sub-plots, and four replications. The main plots contained two bean cultivars, while the sub-plots received two soybean sowing times (0 and 7 days after the beans had been sown), while the sub-sub plots contained five soybean plant densities (0, 5, 10, 20, and 40 plants m-2). The tolerance of the bean genotypes was evaluated with two experiments in a completely randomized design with three replications. They were arranged in a 28 x 3 factorial design (bean genotypes x herbicide doses). Each soybean plant per m2 reduced bean crop yield by 4%. The recommended doses of ethoxysulfuorn and halosulfuorn resulted in tolerance levels above 70% for all the studied bean genotypes.
Highlights:
The herbicides ethoxysulfuron and halosulfuron, are registered in Brazil for the control of volunteer soybean plants in bean crops.
To date, the impact generated by volunteer soybean plants competing with bean plants is unknown.
The tolerance of 28 bean cultivars to herbicides ethoxysulfuron and halosulfuron was determined using dose label and double the dose.
The impact of volunteer soybean plants on bean crops was determined using two bean cultivars and two soybean sowing times.
The bean genotypes displayed a highly variable response to the herbicides ethoxysulfuron and halosulfuron; however, when the label recommended dose of the herbicides was used, the tolerance levels observed were over 70%.
Each soybean plant is capable of causing a 4% reduction in bean plant grain yield, regardless of the establishment time of the soybean plants or the bean genotype
Control capacity of the LPSc 1067 strain of Beauveria bassiana (Ascomycota: Hypocreales) on different species of grasshoppers (Orthoptera: Acrididae: Melanoplinae), agricultural pests in Argentina
Full text linkGrasshoppers affect agriculture worldwide, causing serious economic damage. Currently, the application of chemical insecticides against grasshoppers is the only effective strategy, even considering the significant environmental concern. This study aimed to test the entomopathogenic fungi Beauveria bassiana (LPSc 1067) as biocontrol agent on six harmful grasshopper species in Argentina. Significant differences were observed (DF= 5; F= 9.93; P<0.0001) when considering B. bassiana pathogenicity on third-instar nymphs of the different grasshopper species. The highest mortality (100%) was registered on Trimerotropis pallidipennis and Dichroplus maculipennis nymphs while the lowest mortality (48.6 ±3.5%) was observed on Scotussa lemniscata nymphs. The lowest mean survival time (MST) was recorded for T. pallidipennis (3.5 ±0.15 days) and the highest MST was observed on Dichroplus pratensis nymphs (7.48 ±0.28 days). Results suggest that B. bassiana LPSc 1067 may constitute an excellent candidate to be further studied as biological control agent of T. pallidipennis and D. maculipennis.
Highlights:
Melanoplinae grasshoppers include several species considered plagues.
Entomopathogenic fungi as biocontrol agents are considered excellent alternatives to chemical control.
The efficacy of bassiana (LPSc 1067) on six grasshopper species of Argentina.
The isolate LPSc 1067 could be considered a biological controller pallidipennis and D. maculipennis.Grasshoppers affect agriculture worldwide, causing serious economic damage. Currently, the application of chemical insecticides against grasshoppers is the only effective strategy, even considering the significant environmental concern. This study aimed to test the entomopathogenic fungi Beauveria bassiana (LPSc 1067) as biocontrol agent on six harmful grasshopper species in Argentina. Significant differences were observed (DF= 5; F= 9.93; P<0.0001) when considering B. bassiana pathogenicity on third-instar nymphs of the different grasshopper species. The highest mortality (100%) was registered on Trimerotropis pallidipennis and Dichroplus maculipennis nymphs while the lowest mortality (48.6 ±3.5%) was observed on Scotussa lemniscata nymphs. The lowest mean survival time (MST) was recorded for T. pallidipennis (3.5 ±0.15 days) and the highest MST was observed on Dichroplus pratensis nymphs (7.48 ±0.28 days). Results suggest that B. bassiana LPSc 1067 may constitute an excellent candidate to be further studied as biological control agent of T. pallidipennis and D. maculipennis.
Highlights:
Melanoplinae grasshoppers include several species considered plagues.
Entomopathogenic fungi as biocontrol agents are considered excellent alternatives to chemical control.
The efficacy of bassiana (LPSc 1067) on six grasshopper species of Argentina.
The isolate LPSc 1067 could be considered a biological controller pallidipennis and D. maculipennis.
Health risk due to pesticide exposure in tomato (Solanum lycopersicum) crop in Oaxaca, Mexico
Full text linkPesticides increase agricultural productivity worldwide. Unfortunately, these pesticides put public health and the environment at risk. This study aimed to document the presence of pests and diseases in tomato crops, the range of pesticides used, and acute pesticide poisoning symptoms (APP) among producers from various municipalities in the State of Oaxaca, Mexico. Surveys were applied from 2019 to 2021. The information was examined through a descriptive analysis. The Mann-Whitney U test and Spearman’s Rho correlation established differences between groups and associations. The main pests were the white fly, various worms, blight, mildew, and weeds. Fifty-five active ingredients (AI) were identified, predominantly Toxicological Category (TC) IV, such as insecticides and fungicides, as well as TC III herbicides. Factors associated with a greater diversity of AI were <10 years in agricultural activity, high presence of pests and diseases, and surfaces >1 ha. Up to six APP symptoms occurred in 60.6% of the producers, and 58.2% of the AI identified are considered hazardous pesticides.
Highlights:
Fifty-five active ingredients were identified in tomato crops, predominantly insecticides and fungicides.
More than fifty percent of the active ingredients identified are considered hazardous pesticides.
Up to six acute pesticide poisoning symptoms occurred in 60.6% of the producers.Pesticides increase agricultural productivity worldwide. Unfortunately, these pesticides put public health and the environment at risk. This study aimed to document the presence of pests and diseases in tomato crops, the range of pesticides used, and acute pesticide poisoning symptoms (APP) among producers from various municipalities in the State of Oaxaca, Mexico. Surveys were applied from 2019 to 2021. The information was examined through a descriptive analysis. The Mann-Whitney U test and Spearman’s Rho correlation established differences between groups and associations. The main pests were the white fly, various worms, blight, mildew, and weeds. Fifty-five active ingredients (AI) were identified, predominantly Toxicological Category (TC) IV, such as insecticides and fungicides, as well as TC III herbicides. Factors associated with a greater diversity of AI were <10 years in agricultural activity, high presence of pests and diseases, and surfaces >1 ha. Up to six APP symptoms occurred in 60.6% of the producers, and 58.2% of the AI identified are considered hazardous pesticides.
Highlights:
Fifty-five active ingredients were identified in tomato crops, predominantly insecticides and fungicides.
More than fifty percent of the active ingredients identified are considered hazardous pesticides.
Up to six acute pesticide poisoning symptoms occurred in 60.6% of the producers
PCR identification of lactic acid bacteria populations in corn silage inoculated with lyophilised or activated Lactobacillus buchneri
Full text linkThis study aimed to evaluate the effect of inoculation with lyophilised and/or activated Lactobacillus buchneri on lactic acid bacteria populations in corn silage. Experimental treatments consisted of corn silage without additives or silage with the inoculants of L. buchneri (1 x 105 cfu/g) applied according to the manufacturer’s recommendations (1 g/tonne fodder) in the forms of the lyophilised inoculant and pre-activated inoculant. Purified isolates from corn silage with and without the inoculant were identified, and 93% of the isolates corresponded to the lactic acid bacteria of the species Lactobacillus plantarum. Among the isolates, no bacteria of the species L. buchneri were detected. The application of lyophilised or activated L. buchneri improved the microbiological profile and reduced ethanol production in corn silage, even without being identified among the isolates captured 70 days after ensilage.
Highlights:
Lactic acid bacteria showed greater development at 7 days of fermentation.
Lactobacillus plantarum predominated at 70 days, representing 93% of the total LAB population.
Lactobacillus buchneri improved its microbiological profile with decreased ethanol production.This study aimed to evaluate the effect of inoculation with lyophilised and/or activated Lactobacillus buchneri on lactic acid bacteria populations in corn silage. Experimental treatments consisted of corn silage without additives or silage with the inoculants of L. buchneri (1 x 105 cfu/g) applied according to the manufacturer’s recommendations (1 g/tonne fodder) in the forms of the lyophilised inoculant and pre-activated inoculant. Purified isolates from corn silage with and without the inoculant were identified, and 93% of the isolates corresponded to the lactic acid bacteria of the species Lactobacillus plantarum. Among the isolates, no bacteria of the species L. buchneri were detected. The application of lyophilised or activated L. buchneri improved the microbiological profile and reduced ethanol production in corn silage, even without being identified among the isolates captured 70 days after ensilage.
Highlights:
Lactic acid bacteria showed greater development at 7 days of fermentation.
Lactobacillus plantarum predominated at 70 days, representing 93% of the total LAB population.
Lactobacillus buchneri improved its microbiological profile with decreased ethanol production