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Data associated with the publication: Global protein dynamics as communication sensors in peptide synthetase domains
No full textData accompanying “Global protein dynamics as communication sensors in peptide synthetase domains.” The data was used to demonstrate the function of protein dynamics in molecular recognition and allostery. We used the Yersiniabactin Synthetase cyclization domain Cy1 as a model system and predominantly employed NMR. The data was used to determine the solution structure of Cy1. For wild-type and the mutant D391N, the data was used to monitor the molecular response of Cy1 when presented to its partner carrier protein ArCP as ArCP is modified from holo to substrate-loaded form. Other data was used to describe Cy1 dynamics through relaxation dispersion and Hahn-Echo, assess its stability through thermal melts, and compare Cy1 wild-type and mutant data
Data associated with the publication: Therapeutic drug distribution across the mouse brain is heterogeneous as revealed by in vivo, spatially resolved aptamer-based sensing
No full textDrug discovery for central nervous system (CNS) targets is a high stakes process with estimated success rates below ten percent. Dose scaling, penetration through the blood–brain-barrier (BBB), and potency are among the various challenges involved in developing drugs for CNS targets. The standard approach to evaluate some of these parameters is dosing lead therapeutic compounds via intravenous delivery and assessing their brain levels via tissue homogenization and ex vivo quantification. Although a cost and time effective approach, brain homogenization lacks pharmacokinetic spatial resolution and normalizes drug levels to the entire brain volume. The brain, however, is known to have regional differences in cellular composition, transporters, BBB permeability, and drug-metabolizing enzymes, factors that could significantly affect pharmacological assessments during drug discovery. In this study we employ electrochemical aptamer-based sensors, a technology that allows in situ, real-time molecular monitoring in live animals, to reveal significant differences in the pharmacokinetics of drug uptake and accumulation in the brain of mice. Using vancomycin in the context of penetrating brain injury (PBI), our results highlight that potency may be significantly affected by PBI location. Additionally, more accurate dose scaling and delivery for deep brain wounds could be achieved by adjusting route of administration based on real-time-measured pharmacokinetic profiles, for example by changing delivery from intravenous to intracerebroventricular dosing. We emphasize the issue of establishing accurate pharmacological parameters during preclinical drug discovery efforts and underline the value of aptamer-based sensors for precise estimations of drug pharmacokinetics, transport across the BBB, and effective dose delivery during preclinical trials
Cross-National Time-Series Data Archive (CNTS) 1815 - 2024
No full textThe Cross-National Time-Series (CNTS) Data Archive is a longitudinal dataset offering over 200 years of annual country-level data spanning from 1815 to 2024. Covering more than 200 nations, the dataset includes 196 variables across demographic, political, legislative, economic, social, and conflict-related domains. Researchers can analyze diverse topics, including socio-economic indicators, political stability, legislative effectiveness, international status rankings, urbanization, communication technologies, trade, military activity, education enrollment, and industrial production
Data and code associated with the publication: Specific functional connectivity of molecular subtypes of subplate and layer 6b neurons
No full textThe deposited data includes MATLAB scripts (.m files) and the corresponding data (.m files or .xlsx files) required to reproduce the figures in the publication. Fluorescence captured images and reconstructed neuron tracings (.tif/tiff files) were also included
Data and code associated with: Phase-Field Fracture Simulation Dataset: Hyperelastic Multi-Crack Response Under Loading and Unloading
No full textThis project aims to develop a benchmark dataset for phase-field fracture mechanics, focusing on fracture propagation of multiple initial defects on a 2D hyperelastic domain with a spherical inclusion under quasi-static cyclic loading. The numerical model captures the non-linear stress-strain behavior of the hyperelastic material. The dataset consists of simulations for 1000 distinct loading conditions applied as boundary constraints, providing detailed insights into crack initiation and propagation patterns. This will be a valuable resource for validating computational methods and advancing data-driven approaches in fracture mechanics
Data associated with the publication: Diffusion, density, and defects on spheres
No full textData for this paper, including example simulation trajectories on both flat and curved surfaces as well as MSD curves, RDF curves, and topological defect distributions averaged over many such simulations. Additionally, this analysis and plotting scripts (all in python)
Data associated with the publication: Trends and enrollment in Medicare Advantage “affinity plans”
No full textThe dataset includes detailed methods and a table containing plans identified in the paper Trends and Enrollment in Medicare Advantage “Affinity Plans.
Data associated with the publication: Conserved transcriptional regulation by BRN1 and BRN2 in neocortical progenitors drives mammalian neural specification and neocortical expansion
No full textThe neocortex varies in size and complexity among mammals due to the tremendous variability in the number and diversity of neuronal subtypes across species. The increased cellular diversity is paralleled by the expansion of the pool of neocortical progenitors and the emergence of indirect neurogenesis during brain evolution. The molecular pathways that control these biological processes and are disrupted in neurological disorders remain largely unknown. Here we show that the transcription factors BRN1 and BRN2 have an evolutionary conserved function in neocortical progenitors to control their proliferative capacity and the switch from direct to indirect neurogenesis. Functional studies in mice and ferrets show that BRN1/2 act in concert with NOTCH and primary microcephaly genes to regulate progenitor behavior. Analysis of transcriptomics data from genetically modified macaques provides evidence that these molecular pathways are conserved in non-human primates. Our findings thus demonstrate that BRN1/2 are central regulators of gene expression programs in neocortical progenitors critical to determine brain size during evolution
Data and code associated with the publication: Direct measurements & simplified models of interfacial colloidal interactions & diffusion with adsorbed macromolecules
No full textData for this paper, including: raw videos (.seq files), custom analysis codes (FORTRAN and MatLab) and analyzed values (.txt files)
PMA2020 Burkina Faso Round 5 Household & Female Survey (2018)
No full textBurkina Faso Round 5 Household and Female (HQFQ) survey used a two-stage cluster design with urban-rural strata. A sample of 83 enumeration areas (EAs) was drawn from the l’Institut National de la Statistique et de la Démographie (INSD) master sampling frame. In each EA, 35 households were selected. Households were systematically selected using the "Random Number Generator" application. Occupants in selected households were enumerated, and eligible women (women of reproductive age, 15-49) were contacted and consented for interviews. Data collection was conducted between November 2017 and January 2018. The final sample included a total of 2,811 households and 3,556 females. More information about this dataset can be found in the corresponding codebook, accessible at https://doi.org/10.34976/b88s-zx32</a