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Data associated with the publication: Charge trapping in polymer electrets with highly dilute blended aryl-amine donors
No full textDevice current-voltage data organized by additive compound, then by percent of additive in transistor gate dielectrics, then by charging behavio
Data associated with the publication: Reaction path model of the formation of abiotic immiscible hydrocarbon fluids in subducted carbonated serpentinites, Lanzo Massif (Western Italian Alps)
No full textThis data file contains supplementary information of the manuscript titled of “Reaction Path Model of the Formation of Abiotic Immiscible Hydrocarbon Fluids in Subducted Carbonated Serpentinites, Lanzo Massif (Western Italian Alps)”. The model simulated H2 fluid reacting with carbonated serpentinites consisting of aragonite and serpentinite. The initial rock compositions are 15 vol% aragonite and 85 vol% serpentinite; 30 vol% aragonite and 70 vol% serpentinite; and 50 vol% aragonite and 50 vol% serpentinite. The reactions were modeled at 400 to 500 °C and 1.0 to 2.0 GPa with fluid/rock (F/R) ratio of 0.1 and trace amount of initial C in fluids. The word document contains the description of model files and tables about the summary of the initial and final fluid compositions and mineral assemblages. The files contain model results at 2.0 GPa 400 °C, 1.0 GPa 400 °C, 1.5 GPa 400 °C, 2.0 GPa 450 °C, and 2.0 GPa, 500 °C. Each file consists of the EQ6 code package including DATA0, data1, EQ6 code, input, output, pickup, tab, and tabx; and the initial fluid composition in the EQ3run_AVB_ophicarb folder
Data associated with the publication: Palmitoylation regulates neuropilin-2 localization and function in cortical neurons and conveys specificity to semaphorin signaling via palmitoyl acyltransferases
No full textNeuropilins (Nrps) are S-palmitoylated in vitro and in vivo in the central nervous system. S-palmitoylation of select Nrp2 cysteines confers subcellular localization specificity and is required for semaphorin 3F-dependent dendritic spine pruning in cortical neurons. Distinct palmitoyl acyltransferases mediate Nrp2 and Nrp1 palmitoylation and function, imparting specificity to semaphorin signaling
Data associated with the publication: Dimerization and autophosphorylation of the MST family of kinases are controlled by the same set of residues
No full textThis archive contains the raw data far-UV circular dichroism (CD) data collected on wild-type and variants of the human MST2 kinase domain
Data associated with the publication: Small RNAs and Hfq capture unfolded RNA target sites during transcription
No full textSmall ribonucleoproteins (sRNPs) guide RNA folding, modification, splicing, and processing during transcription. The data associated with this manuscript examine how bacterial Hfq•DsrA sRNA complexes bind to actively elongating rpoS mRNA using single-molecule co-localization co-transcriptional assembly (smCoCoA). The results show that recruitment of Hfq upstream enabled dynamic sampling of rpoS transcripts for segments complementary to DsrA sRNA. Site-specific fluorophores on the DNA template reveal that Hfq•DsrA can target rpoS mRNA soon after the DsrA binding site is transcribed. The results suggest that sRNPs are able to capture a prospective target site before it is masked by folding of the nascent transcript, providing a kinetic advantage to co-transcripitonal targeting over post-transcriptional targeting. This dataset comprises metadata, EMCCD movies of smCoCoA experiments, and analyzed data used to create the figures in this manuscript
Data associated with: Modeling pollinator behavior through survival analysis.
No full textTimeseries data recording the length of time between pollinator arrival intervals. The IDs of the pollinators and the weather data during the time-interval are recorded.
The data is formatted to fit a Poisson distribution and used in time-to-event analysis
Data associated with the publication: The impact of human H3N2 influenza virus reassortment during 2017-18 on increased influenza cases and disease severity: enhanced virus replication and neuraminidase antibody immune evasion
No full textDuring the 2017-18 influenza season in the United States, there was a high incidence of influenza illness and mortality. However, no apparent mutations were identified in the HA gene segment of dominant H3N2 viruses, and the severity of the season could not be solely attributed to a vaccine mismatch. Clinical samples from the Johns Hopkins Center of Excellence for Influenza Research and Surveillance network during the 2016-17 and 2017-18 influenza seasons were sequenced to target influenza A virus. Phylogenetic trees were constructed based on viral whole genome sequences. Representative viral isolates of the two seasons were characterized in immortalized cell lines and human nasal epithelial cell cultures, and demographic data and clinical outcomes of patients were analyzed. Unique H3N2 reassortment events were observed, resulting in two predominant strains in the 2017-18 season: HA clade 3C.2a2 and clade 3C.3a, which had novel gene segment constellations containing gene segments from HA clade 3C.2a1 viruses. The reassortant re3C.2a2 viruses replicated with faster kinetics and to a higher peak titer compared to the parental 3C.2a2 and 3C.2a1 viruses, which may have contributed to the increased severity. The reassortant re3C.3a was able to infect an older population more effectively compared to the parental 3C.3a, perhaps through evasion of preexisting NA antibodies. Our findings suggest that the increased severity of the 2017-18 influenza season was due in part to two intrasubtypes, co-circulating H3N2 reassortant viruses with fitness advantages over the parental viruses. This information may help inform future vaccine development and public health policies
Data associated with the publication: Explaining the decay of nucleic acid-based sensors under continuous voltammetric interrogation
No full textNucleic acid-based electrochemical sensors (NBEs) can support continuous and highly selective molecular monitoring in biological fluids, in vitro and in vivo, via affinity-based interactions. Such interactions afford a sensing versatility that is not supported by strategies that depend on target-specific reactivity. Thus, NBEs have significantly expanded the scope of molecules that can be monitored continuously in biological systems. However, the technology is limited by the lability of the thiol-based monolayers employed for sensor fabrication. Seeking to understand the main drivers of monolayer degradation, we studied four possible mechanisms of NBEs decay: (i) passive desorption of monolayer elements in undisturbed sensors, (ii) voltage-induced desorption under continuous voltammetric interrogation, (iii) competitive displacement by thiolated molecules naturally present in biofluids like serum, and (iv) protein binding. Our results indicate that voltage-induced desorption of monolayer elements is the main mechanism by which NBEs decay in phosphate-buffered saline. This degradation can be overcome by using a voltage window comprised between -0.2 V and 0.2 V vs Ag|AgCl, reported for the first time in this work, where electrochemical oxygen reduction and surface gold oxidation cannot occur. This result underscores the need for chemically stable redox reporters with more positive reduction potentials than the benchmark methylene blue, and able to cycle thousands of times between redox states to support continuous sensing for long periods. Additionally, in biofluids the rate of sensor decay is further accelerated by the presence of thiolated small molecules like cysteine and glutathione, which can competitively displace monolayer elements even in the absence of voltage-induced damage. We hope that this work will serve as a framework to inspire future development of novel sensor interfaces aiming to eliminate the mechanisms of signal decay in NBEs
Data associated with: The Maryland Food System Map
No full textThis dataset contains geospatial data, code, and documentation relevant to the Maryland Food System Map, a web mapping application maintained by the Johns Hopkins Center for a Livable Future between 2012 and 2023. Approximately 500 geospatial data layers that were featured on the application have been preserved here for use in future analyses of the food system in Maryland. The code behind the application has also been preserved in this dataset and can be used to better understand how the application worked and to develop similar applications in the future. The documentation provides more information about the Maryland Food System Map, including both the history of the application and how it was used. There is also metadata about when and where the data for data layers were obtained
Data associated with the publication: Stability of N-Heterocyclic carbene monolayers under continuous voltammetric interrogation
No full textN-Heterocyclic carbenes (NHCs) are promising monolayer-forming ligands that can overcome limitations of thiol-based monolayers in terms of stability, surface functionality and reactivity across a variety of transition metal surfaces. Recent publications have reported the ability of NHCs to support biomolecular receptors on gold substrates for sensing applications, and improved tolerance to prolonged biofluid exposure relative to thiols. However, important questions remain regarding the stability of these monolayers when subjected to voltage perturbations, which is needed for applications with electrochemical platforms. Here, we investigate the ability of two NHCs, 1,3-diisopropylbenzimidazole and 5-(ethoxycarbonyl)-1,3-diisopropylbenzimidazole, to form monolayers via self-assembly from methanolic solutions of their trifluoromethanesulfonate salts. We compare the electrochemical behavior of the resulting monolayers relative to that of benchmark mercaptohexanol monolayers in phosphate-buffered saline. Within the -0.15 to 0.25 V vs Ag|AgCl voltage window, NHC monolayers are stable on gold surfaces, wherein they electrochemically perform like thiol-based monolayers and undergo similar reorganization kinetics, displaying long-term stability under incubation in buffered media and under continuous voltammetric interrogation. At negative voltages, NHC monolayers cathodically desorb from the electrode surface at lower bias (-0.1 V) than thiol-based monolayers (-0.5 V). At voltages more positive than 0.25 V, NHC monolayers anodically desorb from electrode surfaces at similar voltages to thiol-based monolayers. These results highlight new limitations to NHC monolayer stability imposed by electrochemical interrogation of the underlying gold electrodes. Our results serve as a framework for future optimization of NHC monolayers on gold for electrochemical applications, as well as structure-functionality studies of NHCs on gold