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    553 research outputs found

    Data and code associated with the publication: Rapid prototyping of thermoplastic microfluidic devices via SLA 3D printing

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    The manuscript describes a rapid prototyping process for designing microscale features in thermoplastic using SLA 3D printing and hot embossing. In order to validate the approach, microscale features were designed and characterized, and a functional capillaric circuit microfluidic device was fabricated. This dataset provides supporting raw data. The excel sheet includes raw measurement values taken from an optical profilometer and used to compile the plots in the manuscript and supplementary info figures. The Python script was used to determine the feature dimensions of operable capillaric circuits. The STL file was used for producing the device represented in the publication

    Data associated with the publication: RNA compaction and iterative scanning for small RNA targets by the Hfq chaperone.

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    This dataset is related to a manuscript submitted to Nature Communications, describing a one-dimensional target search by small RNAs and Hfq, using single molecule total internal reflection fluorescence microscopy. RNA-guided enzymes must quickly search a vast sequence space for their targets. This search is aided by chaperones such as Hfq, a protein that mediates regulation by bacterial small RNAs (sRNAs). How RNA binding proteins enhance this search is little known. Using single-molecule Förster resonance energy transfer, we show that E. coli Hfq performs a one-dimensional scan in which compaction of the target RNA delivers sRNAs to sites distant from the location of Hfq recruitment. We also show that Hfq can transfer an sRNA between different target sites in a single mRNA, favoring the most stable duplex. We propose that compaction and segmental transfer, combined with repeated cycles of base pairing, enable the kinetic selection of optimal sRNA targets. Finally, we show that RNA compaction and sRNA transfer require conserved arginine patches. We suggest that arginine patches are a widespread strategy for enabling the movement of RNA across protein surfaces

    PMA2020 India (Rajasthan) Round 2 Household & Female Survey (2017)

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    India Round 2 (Rajasthan) Household and Female (HQFQ) survey used a two-stage cluster design with urban and rural and regional strata. A sample of 147 enumeration areas (EAs) was drawn by the International Institute of Population Sciences (IIPS) from the census. Each EA was listed and mapped; 35 households were randomly selected. Occupants in selected households were enumerated and eligible females of reproductive age (15-49) were contacted and consented for interview. Data collection was conducted between February and April, 2017. The final completed sample included 4,986 households and 6,090 females. More information about this dataset can be found in the corresponding codebook, accessible at https://doi.org/10.34976/b88s-zx32</a

    PMA2020 Kenya Round 2 Service Delivery Point Survey (2014)

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    Kenya Round 2 Service Delivery Point (SQ) survey used a two-stage cluster design with county as strata. A sample of 120 enumeration areas (EAs) was drawn by the Kenya National Bureau of Statistics from its master sampling frame. Each EA was listed and mapped. Public facilities were included if a selected EA fell within the catchment area. Private facilities were included if they fell within the boundaries of the EA. Data collection was conducted between November and December, 2014. The final completed sample in Kenya Round 2 was 324 SDPs. More information about this dataset can be found in the corresponding codebook, accessible at https://doi.org/10.34976/vfbp-bz42</a

    PMA Niger Phase 1 Household and Female Survey (2021)

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    PMA Niger Phase 1 (NEP1) Household and Female Survey includes a total of 103 enumeration areas (EAs). The EAs were drawn using the stratified cluster design with urban-rural strata. The results are representative at the national and regional level. The final sample included 3,515 (97.6%) households and 3,633 (95.4%) de facto women which completed the interview. Data collection was conducted between December 2020 and April 2021. More information about this dataset can be found in the corresponding codebook, accessible at https://doi.org/10.34976/cjvx-z226</a

    PMA Ethiopia Panel (2021-2023) Cohort 2: 6-month Follow-up Survey

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    PMA Ethiopia Panel Cohort 2: 6-month Follow-up survey followed women who were eligible to remain in the Panel Cohort 2 study and did not refuse to follow-up at their previous interview. Women who were 5-9 weeks postpartum at baseline who consented to follow-up; women who are pregnant or 0-4 weeks postpartum at baseline, completed the 6-week follow-up survey, and Women pregnant or 0-4 weeks postpartum at baseline, who consented to the 6-week follow up, and had incomplete 6-week forms with results other than “Refused” or “Respondent died” were included in this survey. A total of 1,874 mother or caregivers completed the 6-month follow-up survey conducted between April 2022 and March 2023. Panel women could become ineligible for 6-month follow-up if they refused follow-up at some point, died, or had no live births. More information about this dataset can be found in the corresponding codebook, accessible at https://doi.org/10.34976/zvvy-x402</a

    PMA2020 Indonesia Round 1 Household & Female Survey (2015)

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    Indonesia Round 1 Household and Female (HQFQ) survey used a two-stage cluster design with province at the first and census blocks at the second stage. A sample of 372 enumeration areas (EAs) was drawn by the Indonesian Central Bureau of Statistics (BPS) from its master sampling frame to accommodate an oversample for one province (South Sulawesi with 60 EAs) and one district (Makassar, with 37 EAs). For each EA, 35 households were randomly selected. A random number generating app was used to select households. Households were surveys and occupants enumerated. All eligible females age 15-49 were contacted and consented for interviews. The surveys aimed for a sample size of 3446 females. Data collection was conducted between June and August 2015. The final sample included 11,613 completed households in 371 EAs and 10,301 de facto female interviews. More information about this dataset can be found in the corresponding codebook, accessible at https://doi.org/10.34976/b88s-zx32</a

    PMA Niger Phase 2 Household and Female Survey (2022)

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    PMA Niger Phase 2 (NEP2) Household and Female Survey collects data on knowledge, practice, and coverage of family planning services in 103 enumeration areas (EAs) selected using a multi-stage stratified cluster design with urban-rural strata. The results are representative at the national and regional levels. The final sample included 3,428 (98.8%) households and 3,696 (96.3%) women who completed the interviews for the cross-sectional survey. The data was collected between January and May 2022.Phase 2 panel data collection was conducted among females aged 15-49 at the time of the Phase 2 survey who were interviewed at the baseline survey between December 2020 and April 2021 and consented to follow-up. Of the 3,376 eligible respondents, 13.3% were not reached for follow-up. Of those reached, 2,831 (83.9%) completed the survey, with a response rate of 96.8% among contacted women. More information about this dataset can be found in the corresponding codebook, accessible at https://doi.org/10.34976/cjvx-z226</a

    PMA2020 Nigeria Round 5 Household & Female Survey (2018)

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    Nigeria (National) Round 5 Household and Female (HQFQ) survey used a three-stage sampling approach within a sample of seven states - Anambra, Kaduna, Kano, Lagos, Nasarawa, Rivers, Taraba. One state per zone was selected using probability proportional to size from among each of Nigeria’s six zones. The seventh state (Kaduna) was allocated to the northwest zone. A total of 302 clusters of enumeration areas (EAs) were drawn from the National Population Commission’s master sampling frame. In each cluster of EAs, households and private health facilities were listed and mapped; 35 households (40 in Lagos) were selected per cluster of EAs. Occupants in selected households were enumerated and eligible females of reproductive age (15-49) were contacted and consented for interview. The final sample included 10,070 households and 11,284 females. Data collection was conducted between April and May 2018 in all states. More information about this dataset can be found in the corresponding codebook, accessible at https://doi.org/10.34976/b88s-zx32</a

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