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Data associated with the publication: Syngap1 and the development of murine neocortical progenitor cells
No full textSYNGAP1 is a major regulator of synaptic plasticity through its interaction with synaptic scaffold proteins and modulation of Ras and Rap GTPase signaling pathways. SYNGAP1 mutations in humans are often associated with intellectual disability, epilepsy, and autism spectrum disorder. Syngap1 heterozygous loss-of-function in mice results in impaired LTP, premature maturation of dendritic spines, learning disabilities and seizures, reflecting the phenotypes of the human SYNGAP1 syndrome. More recently, SYNGAP1 was shown to influence cortical neurogenesis and the proliferation of progenitors in human organoids. Here, we show that the absence or haploinsufficiency of Syngap1 does not influence the properties of neocortical progenitors and their cellular output in mice. Despite careful histological analysis of progenitor numbers, division mode, and neocortical thickness, using validated staining methods and EdU labeling with positive controls, we failed to replicate the main findings from human organoids. This discrepancy highlights potential species-specific or methodological differences and raises significant questions about the broader relevance of SYNGAP1’s role in neurogenesis. The absence of cortical progenitor deficits in Syngap1 haploinsufficient mice, which exhibit cognitive deficits and seizures, indicates that these phenotypes do not arise from differences in neurogenesis but are more likely due to SYNGAP1's prominent role in the regulation of synapse function
One Health POE Checklist for Transboundary Zoonotic Disease Preparedness
No full textThe One Health Point of Entry (POE) Checklist is a novel assessment tool developed to evaluate the multisectoral capacities of land border crossings and international airports in response to transboundary zoonotic disease (TZD) threats. Rooted in the One Health (OH) framework, which integrates human, animal, and environmental health sectors, the Checklist is designed to assess preparedness and operational readiness at POEs—critical junctures for trade, travel, and biosecurity.
Developed through a collaborative process involving stakeholders from Iraq and Jordan, the Checklist is structured around the Prevent, Detect, Respond, and Evaluate framework. It draws from international guidance documents and includes 95–109 practitioner-focused questions depending on the POE type (land or air). These questions address topics ranging from personal protective equipment and zoonotic disease surveillance to cross-border communication and response planning. The checklist comes in two versions: the first for land border crossings, and the second developed international airports in mind.
The Checklist offers a standardized, evidence-based approach for governments to assess and strengthen OH capacities at borders, complement existing global health security tools, and inform national-level decision-making for improved biosurveillance and emergency response
Data and code associated with the publication: Distinct distribution of subplate neuron subtypes between the sensory cortices during the early postnatal period
No full textThe deposited data includes MATLAB scripts (.m files) and the corresponding data (.m files) required to reproduce the figures in the publication. Fluorescence captured images for each figure was also included for better visualization
Data and code associated with the publication: Phase contrast coronary blood velocity mapping with both high temporal and spatial resolution using triggered Golden Angle rotated Spiral k-t Sparse Parallel imaging (GASSP) with shifted binning
No full textThis work proposes a novel MRI method to measure blood flow and vessel cross-sectional area of the coronary arteries with a higher temporal and spatial resolution compared to currently available methods. The proposed methods includes both a new MRI acquisition method using a triggered golden angle (GA) scheme and a k-t sparse parallel imaging reconstruction algorithm that uses shifted binning that minimizes gaps in k-space to improve image quality. The method is tested in the right coronary artery (RCA) and the left anterior descending (LAD) artery of eight healthy subjects in an IRB approved study, and compared against current methods that have either high spatial or high temporal (but not both) resolutions
Data and code associated with the publication: Patchy harmonic functional connectivity of the mouse auditory cortex
No full textAnalyzing the functional connectivity of the brain is an enormous challenge, as deciphering functional connectivity requires knowledge of functional responses and connections. One promising strategy is analyzing the spatial pattern of activity correlations across cell populations. In the primary auditory cortex (A1), cells respond to different sound features. On the large scale, there exists a tonotopic map, which is fractured at the small scale, raising the question of whether functional connections are spatially ordered or
disordered. To test whether functional connectivity on a local and a global scale is also disordered, we first designed a robust statistical model to estimate parameters and test for the significance of the estimated correlation maps. We developed an inference method that allows efficient model fitting and statistical testing to project the correlation maps to 2D space. We then performed in vivo two-photon calcium imaging in Layer 2/3 of A1 with pure tones (PT) or a combination of two tones (TT; harmonically related or not). We found that the spatial patterns of signal (SC) correlations depend on the type of sound stimuli that were presented. The functional 2D maps of PT-driven SCs are more restricted to local neurons than TT signal correlations which showed more global textures. 2D SC patterns for harmonic stimuli showed spatially distinct relationships. TT SCs revealed spatially precise functional connectivity between harmonically related neurons. Thus, even though the frequency preference of neighboring neurons in A1 is functionally diverse, the functional connection pattern of these neurons is functionally precise and harmonically related
PMA Democratic Republic of Congo (Kinshasa) Phase 4 Service Delivery Point Follow-up 4 Survey (2024)
No full textPMA Democratic Republic of Congo (Kinshasa) Phase 4 Service Delivery Point Follow-up #4 Survey includes services in 57 enumeration areas (EAs) in Kinshasa using a multi-stage stratified cluster design. The results are representative at the provincial level. The final sample included 207 SDPs in Kinshasa which completed the interview. Data collection was conducted between June and August 2024. More information about this dataset can be found in the corresponding codebook, accessible at https://doi.org/10.34976/f0vf-qd73</a
Data and code associated with the publication: Monitoring HIV antiretroviral therapy via aptamer-based measurements in preclinical animal models and in human plasma
No full textMonitoring the concentration of antiretroviral drugs is critical to ensuring patient adherence to HIV treatment and prevention regimens, which is crucial for drug efficacy. These tools may also be useful for screening samples at blood donation centers to avert potential new infections. The current benchmark method to support antiretroviral drug monitoring, liquid chromatography coupled to mass spectrometry (LC-MS), requires centralized facilities with costly instrumentation, and has blood-to-result turnaround times of days to weeks, making it impractical for effective drug monitoring. Seeking to overcome this issue, we developed an aptamer for the antiretroviral drug emtricitabine, which is present in most antiretroviral combination therapies in the market and used for both infection management and prevention. The aptamer has clinically relevant sensitivity in biofluids and is highly selective relative to close analogues such as cytosine, cytidine, fluorocytidine and lamivudine (a.k.a. 3TC). Using this aptamer, we developed two analytical assays, one for continuous, in-vivo emtricitabine monitoring in rodent research models, and one for rapid and high-throughput screening of emtricitabine levels in human plasma. Through blinded analytical validation, our clinical assay achieved an 86.9% positive and 100% negative correlation, with an overall agreement rate of 95% relative to the benchmark LC-MS method
Data associated with the publication: Prediction of sulfur-iron redox systematics during mantle wedge metasomatism by slab fluids
No full textThis dataset contains files used for and generated by the EQ3 and EQ6 software (Wolery, 1992), and constitute the results of thermodynamic modeling used to calculate aqueous speciation of fluids and fluid-rock reactions at high pressures and temperatures based on thermodynamic properties consistent with the Deep Earth Water model (Huang & Sverjensky, 2019), including the recently revised properties of sulfur-bearing species (Beaudry & Sverjensky, 2024). The “EQ3_calculations” folder contains files that describe the equilibrium of slab fluids with mafic subducting slabs with an eclogite mineralogy, and the “EQ6_calculations” folder contains the results of reaction path modeling of EQ3-generated fluids reacting with mantle minerals up a thermal gradient from 650°C to 950°C. The “Andradite_calibration” folder contains the results of equilibrium calculations consistent with the study of Gerrits et al. (2019), who report garnet compositions for blueschist-eclogite rocks at a pressure of 2 GPa, temperatures of 470–585°C and log fO2 averaging FMQ+3.2. We used these natural constraints to calibrate the dissolution constant K of the andradite endmember of garnet solid solution (Ca3Fe(III)2Si3O12). We adopted the correction factor to log K of +0.65 to andradite in our EQ3 and EQ6 modeling. The results of EQ6 calculations are used to construct figures 5-12 of the main text, using different initial fluids with variable concentrations of sulfur and/or carbon, variable initial log fO2 (FMQ+1 or FMQ+2), and variable pressure (2 GPa or 3 GPa)
Data associated with the publication: State Medicaid policies on mobile crisis teams: a national policy mapping analysis
No full textThis dataset accompanies a national policy mapping study of Medicaid guidance for mobile crisis team (MCT) services across all 50 states and the District of Columbia. It includes PDFs of state Medicaid documents (provider manuals, waivers, toolkits), as well as Excel files that code the presence or absence of 53 policy features across 15 domains. The study identifies patterns and gaps in clarity and structure of Medicaid guidance for behavioral health crisis response. Supplemental materials include coding reliability statistics and narrative descriptions. For Nevada and West Virginia, publicly available documents were not available and are not included in this collection
Code associated with the publication: Evaluating the role of alpha cell dysregulation in the progression to type 2 diabetes using mathematical simulations
No full textCode used in the mathematical modeling of the role of alpha-cell dysregulation on the longitudinal progression to T2D