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Data associated with the publication: Cooling-induced cutaneous vasodilatation is mediated by small-conductance, calcium-activated potassium channels in tail arteries from male mice
No full textWe previously demonstrated that physiological cooling of cutaneous arteries causes dilation by increasing communication through myoendothelial gap junctions, which allows hyperpolarization initiated by the opening of endothelial calcium-activated potassium channels (KCa) to be conducted directly to the smooth muscle cells resulting in dilation. The goal of the present study was to determine the individual roles of endothelial KCa with small (SK3) and intermediate (IK1,SK4) conductance in this cooling induced dilation.
The study involved functional analyses to directly study the cooling-induced dilation, along with biochemical and immunofluorescent imaging analysis to evaluate the expression and localization of SK3 and IK1/SK4 channels. For functional analysis, vasomotor responses of mice isolated cutaneous tail arteries were analyzed by pressure myography at 37°C and 28°C. Cooling-induced dilation was assessed as the cooling-induced increase in endothelium-dependent dilation to acetylcholine and the cooling-induced inhibition of constrictor responses to U46619. The roles of SK3 and IK1/SK4 channels in the responses to cooling were assessed by evaluating the effects of selective pharmacological inhibition of SK3 (UCL1684) or IK1/SK4 (TRAM34). Expression of SK3 and IK1/SK4 was evaluated using immunoblot analysis of arterial lysates. Immunofluorescent analysis was performed by laser scanning microscopy on arterial segments to determine expression of SK3 and IK1/SK4 and their potential localization to holes in the internal elastic lamina, which is the site of myoendothelial junctions
Data and code associated with the publication: Simple models of directly measured energy landscapes for different shaped particles in nonuniform AC electric fields
No full textData for this paper, including tracked particle trajectories (.mat) and custom analysis codes (.mat) for calculating measured energy landscapes via “Boltzmann Inversion” for anisotropic particles
Data associated with: Critical Responses to Anti-Asian Violence (CRAAV) Focus Group Project
No full textCritical Responses to Anti-Asian Violence (CRAAV) is a scholarly and community-oriented initiative founded by Erin Aeran Chung (Political Science, Johns Hopkins University), Clara Han (Anthropology, Johns Hopkins University), and H. Yumi Kim (History, Johns Hopkins University) in Fall 2021 to build anti-racist coalitions across Johns Hopkins University and Baltimore. It takes anti-Asian violence as a site at which to develop intersectional frameworks to engage the heterogenous challenges facing AAPI communities and interrogate the effects of white supremacy on academic knowledge produced about minoritized communities. An integral component of the initiative is our community partnerships, through which we have sought to identify the most pressing areas of concern in the Baltimore-area AAPI communities. In Spring 2023, students in Professor Erin Chung’s Comparative Racial Politics class engaged in a project seeking to document the voices of the community through a pilot AAPI focus group interview. They began by identifying key community and advocacy organizations in the Baltimore area and, from there, conducting individual interviews with leaders from a few of the organizations. On April 19, 2023, Professor Chung and her students conducted a focus group interview with 8 AAPI residents whom they recruited from the organizations. Topics of discussion included their experiences living as AAPI residents in the Baltimore area, their relationships with community organizations, and the central community issues that affect AAPI residents. The CRAAV Focus Group Project collection contains the focus group transcript. In order to ensure the confidentiality of the focus group participants, all personal identifiers have been removed from the transcripts in the collection, including participants’ names, self-introductions at the beginning of the focus groups, and informal conversations that either contained personal information or that were between participants and not meant to be directed at the group
One Health Transboundary Assessment for Priority Zoonoses
No full textThe One Health Transboundary Assessment for Priority Zoonoses (OHTAPZ) tool is an assessment tool for cross-sectoral prioritization of transboundary zoonotic diseases (TZDs), and mapping of systems for One Health coordination at formal border crossings.
The tool uses a phased approach to bring together multisectoral stakeholders, create a consensus list of priority TZDs, identify existing processes for communication and coordination across and between sectors and levels, analyze strengths and weaknesses of existing operations, and recommend actions to address gaps in coordination from the local, subnational, national, and transboundary levels. OHTAPZ adds to the toolkit of modular, flexible, and easily adaptable approaches to One Health systems assessments that can support national, bilateral/regional capacity strengthening, regional epidemic preparedness, and compliance with international frameworks. See Center for Health Security: current projects. Provided is a copy of the manual and all appendices that are referenced in the manual, which can be downloaded individually in the "Files" tab section. To download the manual and all appendices as a single zip folder, choose “2025-10-OHTAPZ-JHRDR with Appendices.
Data associated with the publication: Dysregulated fatty acid metabolism following intermittent hypoxemia-induced neonatal brain injury is rescued by treatment with acetate
No full textThe dataset is from unbiased proteomic analysis of normoxia and intermittent hypoxic injured hippocampus of mice (Mus musculus) at postnatal day 11
Data associated with the publication: Adapting antibody-invertase fusion protein immunoassays to multiwell plates for infectious disease antibody quantification
No full textTraditional enzyme-linked immunosorbent assays (ELISAs) rely on horseradish peroxidase (HRP)-conjugated antibodies to generate a colorimetric response proportional to target antibody concentration. However, spectrophotometric quantification requires expensive benchtop equipment, limiting its usability for frequent, population-scale immunity screening. To overcome this barrier, we previously developed LC15, an antibody-invertase fusion protein that catalyzes sucrose-to-glucose conversion in proportion to antibody levels. This fusion protein enabled antibody quantification using handheld glucometers – affordable, widely available devices already integrated with telehealth infrastructure. Unlike commercial ELISAs, which report relative antibody titers, LC15 facilitates absolute antibody quantification (µg/mL), enhancing applications such as epidemiological monitoring and convalescent plasma dosing. To increase the number of clinical samples processed in a single run of the assay, in this study we transitioned from poly(methyl methacrylate) strips to microwell plates, optimizing pH conditions and reagent concentrations. This adaptation yielded similar sensitivity to the original strip-based assay, but with a 5-fold reduction in reagent consumption and in plasma, as opposed to serum used for the previous study. Using the SARS-CoV-2 receptor binding domain (RBD) as the antigen, we applied LC15 in a 96-well plate format to screen 72 clinical samples in triplicate for anti-RBD antibodies. A blinded comparison with commercial ELISAs demonstrated strong linear correlation (R² = 0.85) over four orders of magnitude in concentration. By combining accuracy with accessibility, this approach has the potential to facilitate population-level immunity assessments, supporting rapid public health responses in future outbreaks.
The clinical data used in our analyses for the efficacy of the method are available in an earlier publication:
https://insight.jci.org/articles/view/178460/sd/3</a
Data associated with the publication: Imitatecholec: A multimodal dataset for long-horizon imitation learning in robotic cholecystectomy
No full textImitateCholec is a publicly available, multi-modal dataset of over 18,000 ex vivo porcine cholecystectomy demonstrations. Each clipping and cutting phase is broken into 17 fine-grained tasks and captured synchronously via stereo endoscopic and wrist-mounted cameras (JPEG frames) together with da Vinci Research Kit kinematics (CSV). Both optimal executions and recovery maneuvers are included, supporting long-horizon imitation learning, workflow modeling, error recognition, and surgical tool pose estimation
Data associated with the publication: Focus group discussions among Black women in Texas to inform a web-based intervention to increase HIV/STI self-testing, linkage to treatment, and linkage to PrEP
No full textThis data is from aim 1 of a NIMH R34 award. We conducted focus group discussions (FGDs) among 24 participants across 8 groups via Zoom. Participants included PrEP-eligible Black, cisgender women living in Texas. FGDs focused on components to inform a 5-session web-based intervention to increase HIV/STI self-testing, linkage to treatment, and linkage to PrEP. The FGD guide began with asking participants about their knowledge of HIV and STIs (i.e., transmission, prevention, treatment) and concern regarding contracting HIV or STIs. Next, we discussed facilitators and barriers to HIV/STI self-testing procedures (mental contrasting), and action plans (implementation intentions) to overcome barriers for each of the 5 intervention sessions: 1) using the HIV/STI self-test, 2) mailing the HIV/STI self-testing kit, 3) obtaining test results, 4) if applicable, obtaining treatment, and 5) linkage to PrEP. Lastly, we discussed potential names for the intervention and recruitment strategies
Data and code associated with the publication: The role of attention in multi attribute decision making
No full textThis dataset includes both behavioral data and neurophysiological recordings from two male rhesus macaques performing a multi-attribute decision making tasks. Monkeys made fixations to two options with two options each presented on a screen in front of them, then indicate their decision by moving a joystick in the direction of their chosen option. Water rewards are then delivered according to the reward amount and probability attributes of the chosen option. Neural recordings are from the pre-supplementary motor area. Spike-sorted single units were obtained over a number of recording sessions for both monkeys
Data associated with the publication: Competition for Hfq drives kinetic selection of mRNA targets by E. coli small non-coding RNAs
No full textSmall non-coding RNAs (sRNAs) regulate gene expression in E. coli bacteria in response to diverse stimuli in the environment and the host. Most sRNAs regulate mRNA expression by directly base pairing with complementary sites in the target mRNA with the help of the chaperone protein Hfq. sRNAs and Hfq must rapidly search hundreds of candidate mRNAs for matched (cognate) targets while discriminating against non-cognate targets. Here, we use single molecule fluorescence microscopy to directly observe how cognate and non-cognate mRNAs bind immobilized sRNA-Hfq. The results show that initially unstable sRNA-Hfq-mRNA complexes either dissociate within seconds by ejecting one of the two RNAs, depending on their interactions with Hfq, or stabilized by sRNA-mRNA base pairing. Cognate mRNAs are more likely to form long-lived sRNA-Hfq-mRNA complexes, even in the presence of competing RNA. Active competition for the mutual Hfq chaperone introduces a kinetic barrier to RNA co-localization that is resolved by base pairing, driving the accumulation of cognate sRNA-mRNA interactions while eliminating non-cognate interactions