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Computer Modelling of the Interaction Between CeRme-8 J Domain and CeHsp70-1
Get PDFAbstract: Rme-81 is a J domain-containing plasma membrane protein that is required for endocytosis in various cells2. The J domain is a characteristic structural motif found mainly in heat shock protein 40 (Hsp40 or DnaJ) and other proteins such as Rme-83. Within the J domain is a tripeptide, the HPD motif, that is required by the J-domain protein to interact with and stimulate the ATPase activity of Hsp70, a major cellular chaperone4. The Rme-8 protein in C. elegans, CeRme-8, has not been identified with a particular Hsp70 partner. CeHsp70-1 is the only cytosolic Hsp70 in C. elegans, therefore, we hypothesize that CeHsp70-1 is the binding partner for the J domain of CeRme-8. To test this hypothesis, we employed computer modelling to predict the interaction between CeRme-8 and CeHsp70-1 using the known DnaK-DnaJ protein complex as a template. In addition, we report the successful cloning and expression of the J domain of CeRme-8. AlphaFold, SwissModel, and Phyre2 modelling programs revealed that CeRme-8 possesses a J domain that contains the canonical HPD tripeptide motif. ClusPro docking program predicted similar binding interface to the DnaK-DnaJ complex, along with several nonconforming models. Complementary DNA of the J domain of CeRme-8 was cloned into the pGEX-Tev-KG plasmid, in-frame with the gene for glutathione-S-transferase (GST), to yield a GST-CeRme-8 fusion protein. IPTG-induced expression of the expected 37- kilodalton fusion protein was confirmed by both SDS-PAGE and western blotting using antibody against GST. Future work will involve purifying and testing the effect of the J domain protein on the ATPase activity of CeHsp70-1
Should Cities Offer Remote Work?
Get PDFIn December 2019, the SARS-CoV-2 virus was discovered in Wuhan, China. This highly contagious virus soon spread rapidly throughout the world and brought many countries and cities to a standstill through lockdowns, travel bans, and fear of illness and death. Many employers abandoned in-person work to prevent the spread of what became known as COVID-19, or COVID. Plans were made instead for employees to work from home as employers created modified work environments and moved meetings to online platforms. In 2022, once the virus was considered to be under control and fear began to subside, employers began to reopen for business and demanded employees return to in-person work. However, the known workplace had been changed forever as it had been proven that employees could successfully work remotely, meaning the flexible and modified workplaces became not only the norm, but the preference, for many employees. This paper will outline the pros and cons for remote workers, and why cities should consider a remote workplace
The Great Unlearning
Get PDFThe Great Unlearning is a conceptual exploration: sifting through experiences and objects to overcome psychological pain, expectations of society, individual upbringing, and outside influences in an ongoing quest for authenticity. To both embrace personal history and honor loved ones while letting go of lingering negativity is challenging. Using objects culled from my life, examining the past, and incorporating items gathered along my path through nature, the work seeks personal healing while promoting the power of all people to break from indoctrination, group think, and mob mentality to make better choices to live a satisfying and peaceful existence…hopefully in a democratic free society
Against the Tide: Indigenous Knowledge and Education for Humanization
Get PDFPower brokers and their market economies enforce education on a global level. According to the United Nations, the effects of global neoliberal capitalism cause human rights violations in all parts of the world, yet democratic countries scoff at these findings (Pogge, 2002 & 2005). People of the world continue to believe that tying minoritized students to existing structures and ensuring enculturation is the best possible outcome for all involved (Suárez-Orozco & Suárez-Orozco, 2015). That is, minoritized children are educated to ensure first-world countries produce a minimally educated and willing labor force. In this paper we argue the following: 1) power relations among global economies lead to displacement and migration (nothing new), and 2) an alternative lens for ameliorating these conditions: Indigenous knowledge as a counterpoint to power relations; toward recreating an egalitarian and socially just society
Chroma Cowboy
Get PDFChroma Cowboy consists of eleven canvases wrapped in used feed sacks layered with vinyl to create brightly colored narrative scenes. Pulling from Pop Art colors, cubism shapes and a consciousness of materials, I designed this body of work to give the hard-working cowboys, ranchers, and agribusinesses a way to have their stories told to a larger audience. The strain on ranches due to generational changes, politics and environmental circumstances makes it even more important to document this way of life. I strive to further advance and inform the general public’s knowledge of the unyielding lifestyle and dedication that it takes to maintain a ranch. Using the transparency and texture provided from the inside of each feed bag, there is a conscious visual relationship between livestock and the food that they eat. Mass produced and commercialized feed sacks represent the efforts ranchers have taken to contemporize this way of life and adapt to the ever-evolving economy. The different scenes depict strenuous labor, idealized landscapes, and provide insight to a fading way of life that few get to experience paired with pops of color that entice the viewer
A Family Matter
No full textAfter the sudden passing of father and husband, Noah Collins, Meredith Foster is left to deal with Noah’s children (her step children), who are not particularly her biggest fans. Tyler Collins, an insecure but fervent sixteen-year-old, not only despises his step-mother, but is also convinced that she had something to do with his father’s death and will stop at nothing to prove her guilty. Lucy Collins, a six-year-old softball prodigy, is a miniature version of her late father and takes his loss the hardest. On top of struggling to deal with her own grief and doing her best to console her step daughter, Meredith must fight to defend her innocence against Tyler and the rest of the town. Yet, is she completely innocent? Set in the fictional town of Cresswind, Texas, A Family Matter is a heartbreaking story of grief, romance, and revenge
Deconstructing Social Constructs: Exploring Teachers’ Positionality When Teaching Race and Human Diversity in the Science Classroom
Get PDFThis article reports on how four urban high school biology teachers’ positionality impacts their experiences when teaching culturally responsive lessons on race and human diversity. Teachers in the study taught a two-week genetics intervention lesson on race and human diversity, then participated in individual and focus group interviews. Interview transcripts were analyzed using thematic analysis. Results reveal that teachers who were empowered by their racial positionality and had prior professional development in culturally responsive pedagogy (CRP) were more likely to believe that science instruction can be used to address unscientific misconceptions amongst high school students. These findings give credence to utilizing CRP training that allows teachers to explore how their positionality can affect ideas related to teaching culturally responsive lessons on race and human diversity
Parent Perceptions of Post-Secondary Programming Completed by Their Adult Children with Autism Spectrum Disorder
Get PDFTexas Tech University\u27s Burkhart Center for Autism Education and Research works with communities & families in the greater West Texas area to provide supports and services to individuals with Autism Spectrum Disorder with a primary focus on building independent living and vocational skills in a post-secondary environment. In this study, parents of individuals with ASD who had completed the Burkhart Center’s Transition Academy completed an online survey. Parents identified areas in which they were satisfied with their child’s post-secondary experience as well as dissatisfaction, and of additional need. The findings of this current study may provide critical information to post-secondary programs who work with students with ASD
Reporting of Doctoral Student Attrition: A Policy Brief
Get PDFRoughly half of all doctoral students in the United States will never complete their degree requirements (Council of Graduate Schools, 2020). That staggering number is larger for ethnic minority and female students, particularly for Black students who have the lowest completion rate (47 percent) and who make up only 13 percent of doctoral degrees (Lovitts, 2001). Additionally, retention rates for online students are an additional 10 to 20 percent lower than students who attend in-person (Rovai & Wighting, 2005). Thus, ethnic minority students in online doctoral programs are at a higher risk of not finishing their degrees compared to other genders and races (American Psychological Association, 2020).
While research has focused on understanding undergraduate student persistence (Astin, 1999; Bean, 1982; Tinto, 1988) graduate student persistence, particularly doctoral student persistence has been largely underresearched. Given the staggeringly low percentage rate of doctoral student completion, particularly in online doctoral programs, more research is needed to explore this phenomenon. These attrition rates have maintained at steady unacceptable levels for decades and research is starting to identify corrective measures. Accountability for reporting retention rates largely does not exist in the U.S. unless reported to a specific academic governing body or by the university itself. There is no mandatory reporting policy for this student data and data for these retention statistics often goes unreported or underreported by means of a self-report to the NCSES each year (NCSES, 2021)