RODBUK Jagiellonian University in Kraków
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3D melanoma spheroid model for the development of positronium biomarker
No full textMelanoma spheroids (lWM 115 WM 266-4)were irradiated by positrons emitted from the 22Na radionuclide (with activity of about 1 MBq) placed in Kapton foil. A dedicated aluminum chamber was used as a container for spheroids and a holder which was connected to a heater to keep cells at 370C. Exemplary experimental lifetime spectrum (black histogram) with superimposed histograms resulting from the fit of the sum of the exponential function convoluted with the detector resolution, performed by means of the PALS Avalanche program. First component (yellow line) shows the contribution from p-Ps (mean lifetime: 0.125 ns), second component (green line) originates from annihilations in source (Kapton foil) (0.374 ns), third component (light blue) shows free annihilation lifetime (0.395 ns), forth component (dark blue) illustrates the contribution from o-Ps. A sum of all contributions resulting from the fit is shown as a red curve
Fine particulate matter-induced oxidative stress mediated by UVA-visible light leads to keratinocyte damage
No full textThe human skin is exposed to various environmental factors including solar radiation and
ambient air pollutants. Although, due to its physical and biological properties, the skin efficiently
protects the body against the harm of environmental factors, their excessive levels and possible synergisticaction may lead to harmful effects. Among particulate matter present in ambient air pollutants,PM2.5 is of particular importance for it can penetrate both disrupted and intact skin, causing adverseeffects to skin tissue. Although certain components of PM2.5 can exhibit photochemical activity, only a limited amount of data regarding the interaction of PM2.5 with light and its effect on skin tissue are available. This study focused on light-induced toxicity in cultured human keratinocytes, which was mediated by PM2.5 obtained in different seasons. Dynamic Light Scattering (DLS) and Atomic Force Microscopy (AFM) were employed to determine sizes of the particles. The ability of PM2.5 to photogenerate free radicals and singlet oxygen was studied using EPR spin-trapping and time-resolved singlet oxygen phosphorescence, respectively. Solar simulator with selected filters was used as light source for cell treatment to model environmental lightning conditions. Cytotoxicity of photoexcited PM2.5 was analyzed using MTT assay, PI staining and flow cytometry, and the apoptotic pathway was further examined using Caspase-3/7 assay and RT-PCR. Iodometric assay and JC-10 assay were used to investigate damage to cell lipids and mitochondria. Light-excited PM2.5 were found to generate free radicals and singlet oxygen in season-dependent manner. HaCaT cells containing PM2.5 and irradiated with UV-Vis exhibited oxidative stress features–increased peroxidation of intracellular lipids, decrease of mitochondrial membrane potential, enhanced expression of oxidative stress related genes and apoptotic cell death. The data indicate that sunlight can significantly increase PM2.5-mediated toxicity in skin cells
Dataset for the study investigating cognitive processes and personality traits underlying four phenotypes of susceptibility to (mis)information
No full textPlik Excel zawiera dane badawcze, niezbędne do przeprowadzenia analizy z badania, którego wyniki zostaną opublikowane w artykule: "Four phenotypes of susceptibility to (mis)information: cognitive processes and personality traits". Finansowanie: Badania prowadzące do uzyskania tych wyników były finansowane ze środków Mechanizmu Finansowego EOG na lata 2014-2021. Numer rejestracyjny projektu: 2019/35/J/HS6/03498. The Excel file contains the research data necessary for the analysis of the study, the results of which will be published in the article: "Four phenotypes of susceptibility to (mis) information: cognitive processes and personality traits". Funding: The research leading to these results has received funding from the EEA Financial Mechanism 2014-2021. Project registration number: 2019/35/J/HS6/03498
0-1 matrix of Pulsatilla patens ISSR markers : regenerants vs initial and maternal plants
No full text0-1 matrix of Pulsatilla patens ISSR markers. Regenerants vs initial and maternal plants. 0 means absence of the band; 1 means presence of the band. Population codes as described in figure caption of Fig. 2. Ł - plants from natural population in Łagiewniki near Busko-Zdrój; BZ - seedlings from seeds harvested in Łagiewniki near Busko-Zdrój; R - regenerated plants from Łagiewniki near Busko-Zdrój seedling explants. Based on this data NeigborNet using Dice distance was constructed
FASTQ files from NGS 16S bacterial DNA sequencing : effect of butyrate and beta-glucan on HFD
No full textFASTQ files from NGS 16S bacterial DNA sequencing - effect of butyrate and beta-glucan on HF
Modulation of ODH propane selectivity by zeolite support desilication : vanadium species anchored to Al-rich shell as crucial active sites
No full textThe data relating to the commercially available zeolites HY and its desilicated analogues. Various spectroscopic techniques (in situ probe molecules adsorption and time-resolved propane transformation FT-IR studies, XAS, 51V MAS NMR, and 2D COS UV-vis) were employed to study speciation, local coordination, and reducibility of the vanadium species introduced into the hierarchical faujasite zeolite. The acid-based redox properties of V centres were linked to catalytic activity in the oxidative dehydrogenation of propane. The modification of zeolite via caustic treatment is an effective method of adjusting its basicity—a parameter that plays an important role in the ODH process. The Al-rich shell was also beneficial for anchoring V species and thus their reducibility. The operando UV-vis experiments revealed higher reactivity of the bridging oxygens V-O-V over the oxo-group V=O. The (SiO)3V=O species were found to be ineffective in propane oxidation when temperature does not exceed 400 °C
Dane surowe zgromadzone w trakcie oceny stanu zachowania księgozbioru oo. kamedułów z Bieniszewa, przechowywanego w Bibliotece Jagiellońskiej
No full textAneks elektroniczny do rozprawy doktorskiej pt. "Ocena stanu zachowania starych druków na podstawie doświadczeń Polski i Rosji : model bibliotekoznawczy". Plik arkusza kalkulacyjnego zawiera dane surowe, uzyskane w trakcie przeprowadzenia oceny stanu zachowania księgozbioru oo. kamedułów z Bieniszewa przechowywanego w Bibliotece Jagiellońskiej (w trybie rozszerzonym). Elementem szeregowania w tabeli jest numer formularza oceny stanu zachowania
Nitrite concentration in the striated muscles is reversely related to myoglobin and mitochondrial proteins content in rats
No full textThe file contains research data for figures in the manuscript by Joanna Majerczak, Agnieszka Kij, Hanna Drzymala-Celichowska, Kamil Kus, Janusz Karasinski, Zenon Nieckarz, Marcin Grandys, Jan Celichowski, Zbigniew Szkutnik, Ulrike Hendgen-Cottaand Jerzy A. Zoladz entitled "Nitrite concentration in the striated muscles is reversely related to myoglobin and mitochondrial proteins content in rats". The manuscript article is under review
Results of the test for T, P and CP symmetry in the decay of ortho-Positronium using the J-PET detector
No full textThe dataset is the final result obtained from the measurements with the J-PET detector to test the sensitivity of testing the T, P, and CP symmetries in the decay of the ortho-Positronium.
Four experiments were conducted to test the discrete symmetries with the J-PET detector.
Two measurements were conducted using a radioactive source (22Na) of 5 MBq and the other two measurements were conducted using the radioactive source (22Na) of 1 MBq
The gut mycobiome - FASTQ files
No full textData includes FASTQ files from the gut mycobiome in children in relation to the clinical activity of Crohn's diseas