RODBUK Jagiellonian University in Kraków
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Tunable Resistive Pulse Sensing results from the isolation of extracellular microvesicles in comparison with the theoretical model
No full textThe data include the size distribution and concentration of EVs were measured by TRPS technology using the qNano system (Izon Science Ltd.,. Oxford,. UK). The instrument was calibrated using CPC100 and CPC200 calibration particles (Izon Science) according to the manufacturer’s instructions. Exosomes were measured using NP100 nanopores (analysis range 50-330 nm; Izon Science) at a pressure of 5 mbar. Ectosomes were measured using NP200 nanopores (analysis range 85-500 nm; Izon Science) with a pressure of 1 mbar. Voltage and stretch were adjusted to obtain a stable current between 100-120 nA. The samples were analyzed for 3 minutes or until the measurement was performed on 500 objects. Data processing and analysis were prepared on the Izon Control Suite v2.2 software (Izon Science).||4 - Łączna liczba autorów publikacjiExtracellular vesicles (EVs) are heterogeneous 30-5000 nm membrane vesicles that transport proteins, non-coding RNAs (miRNAs), lipids and metabolites. Major populations include exosomes, ectosomes and apoptotic bodies. The aim of this work was to compare the distribution of EVs obtained under different conditions of differential centrifugation, including ultracentrifugation, with the results developed on the basis of a theoretical model. This is data set to the article submitted to the journal Bio-Algorithms and Med-Systems (BAMS)
RNA seq data of cardiac fibroblasts after stimulation with WNT3a or WNT5a in the presence of TGFβ
No full textThis data contains lists of Differential Expresses Genes (DEGs) after stimulation of cardiac fibroblasts with TGFβ or TGFβ with WNT3a or TGFβ with WNT5a for 72h
Dataset for the study researching psychological interventions countering misinformation in social media
No full textDataset for the study researching psychological interventions countering misinformation in social media. Funding: The research leading to these results has received funding from the EEA Financial Mechanism 2014-2021. Project registration number: 2019/35/J/HS6/03498
Risk Factors for Hepatotoxicity Due to Paracetamol Overdose in Adults
No full textDatabase for article Risk Factors for Hepatotoxicity Due to Paracetamol Overdose in Adult
Benzo[a]pyrene and benzo[e]pyrene : photoreactivity and phototoxicity towards human keratinocytes
No full textPolycyclic aromatic hydrocarbons (PAHs) are a group of organic compounds derived mostly from the incomplete combustion of fossil fuels and biomass. Human skin can absorb PAHs and the uptake increases with their molar mass and lipophilicity. Benzopyrene is high-molecular-weight PAH frequently appearing in ambient pollution. It exists in two isomeric forms: benzo[a]pyrene (BaP) and benzo[e]pyrene (BeP), which exhibit different biological activity. Although certain properties of benzopyrenes suggested photoreactivity of the compounds, no direct measurements were previously conducted to characterize their photochemical activity. In this study, quantum yield and action spectra of singlet oxygen photogeneration by BaP and BeP were measured by time-resolved near-infrared phosphorescence and the ability of both compounds to photogenerate superoxide anion was assessed by electron paramagnetic resonance (EPR) spin-trapping. The measurements revealed high efficiency of benzopyrenes to photogenerate singlet oxygen and their ability to photogenerate superoxide anion. Using HaCaT cells as single-layer skin model, we demonstrated concentration-dependent and light-dependent cytotoxicity of BaP and BeP. The compounds induced damage to the cell mitochondria and elevated the levels of intracellular reactive oxygen species
FASTQ files from NGS 16S bacterial DNA sequencing - comparison of iSeq with MiSeq
No full textThis metadata contains FASTQ files obtained from NGS 16S bacterial DNA of rat fecal samples performed on 2 platforms: iSeq and MiSeq.
The most import ant similarities and differences of 16S microbiome sequencing were described in article: "Comparison of iSeq and MiSeq as the two platforms for 16S rRNA sequencing in the study of the gut of rat microbiome." (https://pubmed.ncbi.nlm.nih.gov/36322250/
The first online capillary electrophoresis-microscale thermophoresis (CE-MST) method for the analysis of dynamic equilibria – determination of acidity constant of fluorescein isothiocyanate
No full textRaw experimental data and metadata supporting the publication, presented in the Excel file
The gut archeobiome - FASTQ files
No full textarcheobiome sequencing data of children with Crohn's disease - FASTQ file
Data set for the study published in : "When Peppa Pig and Confucius meet, joining forces on the battlefield of health literacy - an analysis of COVID-19 educational materials for children and adolescents from China, the USA, and Europe"
No full textDokument zawiera bazę danych analizowanych dokumentów wraz z linkami do stron internetowych, na których zostały opublikowan