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MURAT NEHRİ MUŞ HAVZASINDA YAŞAYAN Capoeta trutta ve Capoeta umbla BALIKLARININ BAZI DOKULARINDA METALLOTİONEİN PROTEİN DÜZEYLERİNİN BELİRLENMES
No full textAmaç:Bu arştırmada, Capoeta trutta (Heckel, 1843)veCapoeta umbla (Heckel, 1843) türlerinin karaciğer dokularında metallotionein (MT) protein düzeylerinin ölçümü amaçlandı.Materyal ve Metot: Bu çalışmada, Murat Nehri’nde yaşayan Cyprinidae familyası üyesi C. umbla ve C. trutta türlerinden 20 adet yerel balıkçılardan satın alındı (n:10). Balık karaciğer doku örnekleri 0,6 g tartılarak fosfat tamponundan 6 ml eklenerek ultrasonik homojenizatörde ayrıştırıldı (pH 7.4). Plastik tüplere alınan karışım vortekslendi 4 °C’de soğutmalı santrifüjde 9500 rpm’de 30 dakika santrifüj edildi. Ependorf tüplere 1ml süpernatant aktarıldı. MT analizi için Sandwich-ELISA testi kullanıldı. Bu kitin test mikroplaka, teste özgü bir antikorla önceden kaplanmıştır. Standartlar ve örnekler sıraya göre mikroplaka kuyucuklarına eklenerek, spesifik antikorla birleşmesi sağlandı. Her bir mikroplaka kuyucuğuna Horseradish Peroksidaz (HRP) konjuge antikor eklendi, inkübe edildi ve bileşenler yıkandı. Her bir kuyucuğa TMB substrat çözeltisi eklendi. Ölçülen parametreyi ve parametrenin HRP konjuge antikorunu içeren kuyucuklar mavi renkte görünür ve stop çözeltisinin eklenmesi sonrasında sararır. Optik yoğunluk (OD), 450 nm dalga boyunda spektrofotometrik olarak ölçüldü. Numunelerdeki konsantrasyon, numunenin OD'sininin standart eğriyle karşılaştırılması ile hesaplandı. Bulgular: MT düzeyleri C. trutta 0.373±0.006 µg/ml ve C. umbla 0.339±0.019 µg/ml türlerinin karaciğer dokularında ölçüldü (X±SEM Aritmetik ortalama ± Standart hata).Sonuç: Bu çalışmada, C. trutta karaciğer dokusu MT düzeyi, C. umbla karaciğer MT düzeyine göre daha yüksek ölçüldü. Aynı familyadan olmalarına ve aynı ortak yaşam alanında yaşamalarına rağmen bu farklılık balıkların büyüme, gelişme ve beslenme farklılıklarından kaynaklı olabilir.Anahtar Kelimeler:Capoeta trutta, Capoeta umbla,Metallotionein, Murat NehriEtik onay: Van Yüzüncü Yıl ÜniversitesiHayvan Deneyleri Yerel Etik Kurulu'nun 28.11.2019 tarih ve 2019/11 sayılı kararı.Teşekkür: Bu çalışma, Van YYÜ Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından FYL-2020-9286 No’lu proje olarak desteklenmiştir.Aim: The aim of this study was to measure metallothionein (MT) protein levels in liver tissues of Capoeta trutta (Heckel, 1843) and Capoeta umbla (Heckel, 1843) species.Material and Method: In this study, 20 C. umbla and C. trutta species from the Cyprinidae family living in the Murat River were purchased from local fishermen (n: 10). Fish liver tissue samples were weighed as 0.6 g and separated into an ultrasonic homogenizer by adding 6 ml of phosphate buffer (pH 7.4). The mixture taken into plastic tubes was vortexed and centrifuged at 9500 rpm in a refrigerated centrifuge at 4 °C for 30 minutes. 1 ml of supernatant was transferred to Eppendorf tubes. The Sandwich-ELISA test was used for MT analysis. The test microplate of this kit is pre-coated with an antibody specific to the test. Standards and samples were added to the microplate wells to ensure that they combine with the specific antibody. Horseradish Peroxidase (HRP) conjugated antibody was added to each microplate well, incubated and components were washed. TMB substrate solution was added to each well. Wells containing the measured parameter and its HRP conjugated antibody appear blue and turn yellow after the addition of stop solution. Optical density (OD) was measured spectrophotometrically at 450 nm wavelength. Concentration in samples was calculated by comparing the OD of the sample with the standard curve.Results: MT levels were measured in liver tissues of C. trutta 0.373±0.006 µg/ml and C. umbla 0.339±0.019 µg/ml (X±SEMArithmetic average ± Standard error).Conclusion: In this study, C. trutta liver tissue MT levels were measured higher than C. umbla liver MT levels. Although they are from the same family and live in the same common habitat, this difference may be due to differences in growth, development and nutrition of the fish.Key Words: Capoeta trutta, Capoeta umbla, Metallothionein, Murat RiverEthical Statement: Van Yuzuncu Yil University Animal Experiments Local Ethics Committee's decision dated 28.11.2019 and numbered 2019/11.Acknowledgement: This study was supported by Van YYU Scientific Research Projects Coordination Unit as project No. FYL-2020-9286</p
A proof-of-concept practical approach for achieving equivalent results from non-harmonized measurement methods while awaiting harmonization: The CA 125 example
No full textBackground: Laboratory test results are crucial for clinical decisions, yet inconsistencies arise when measurements are not harmonized due to the lack of suitable higher-order references. This study introduces an approach to improve result comparability across different measurement systems, applicable until full metrological harmonization of the measurand is achieved. Methods: A linear transformation formula was developed, utilizing the 2.5th and 97.5th percentiles of data from source and target methods, to adjust source method results. The feasibility of this formula was tested using carbohydrate antigen 125 (CA 125) data from two commercial assays provided by Roche Diagnostics and Abbott Diagnostics. Method comparison statistics, including difference plots and Passing-Bablok regression, were used to evaluate the transformation's effectiveness before and after adjustment. A web application, “Result Transformer,” was developed to facilitate the application of the transformation process. Results: Prior to transformation, the median relative difference between the Roche and Abbott CA 125 assays was 37.7% (95% CI: 34.5–40.8%), exceeding acceptable bias. Passing-Bablok regression yielded a slope of 1.450 (95% CI: 1.400–1.485) and an intercept of −0.83 kU/L (95% CI: −1.50 to −0.29). After adjustment using the proposed approach, the median relative difference decreased to 6.0% (95% CI: 4.3–7.7%), falling within the desirable acceptable bias goal. The slope and intercept of the regression equation improved to 1.075 (95% CI: 1.039–1.102) and −0.12 kU/L (95% CI: −0.71 to 0.19), respectively. Conclusion: The proposed transformation method effectively improved the comparability of results from different assays, permitting a more consistent test result interpretation during patient follow-up
ELUCIDATION OF THE MECHANISM OF CARFILZOMIB-INDUCED CARDIOTOXICITY IN HUMANS THROUGH NETWORK TOXICOLOGY AND BIOINFORMATICS
No full textON THE HECKE EIGENFORMS HALF INTEGRAL WEIGHT BY RANKIN-COHEN BRACKETS
No full textThe systematic selection of Hecke eigenforms oflevel Γ0(4) and half-integral weight is a crucial step inadvancing computational problems in modular forms, such as the Sato–Tateconjecture. In particular, having access to a large number of Fouriercoefficients is essential. To achieve this, concrete examples are needed. Oneeffective approach is to employ Rankin–Cohen (RC) brackets.This talk will begin with essential definitions:modular forms of half-integral weight, Hecke eigenforms, RC brackets, and theKohnen plus space. We emphasize the effective use of RC brackets applied toEisenstein series and theta series, which are fundamental examples of modularforms of both integral and half-integral weights.We will present explicit examples of Heckeeigenforms of half-integral weight obtained via RC brackets of Eisenstein andtheta series. The method is grounded in linear algebraic techniques, and itsvalidity relies on an important theoretical result known as the Sturm bound,which facilitates arithmetic with modular forms.This approach enables efficient computation ofmany Fourier coefficients using algebra systems such as Pari/GP, Magma, orSageMath. We will conclude the talk by discussing the subtle differencesbetween these computational platforms.This talk is joint work with Ilker Inam(Turkey), Elif Tercan (Turkey), Banu Irez Aydın (Turkey), and Gabor Wiese(Luxembourg) and supported by Turkish Research Council (Tübitak) Project number118F148.</p
Comparative clinical efficiency of resveratrol, allopurinol, and resveratrol plus allopurinol in canine visceral leishmaniasis
No full textObjective To assess the clinical and immunological effectiveness of resveratrol alone or combined with allopurinol for treating canine visceral leishmaniasis (CVL). Methods This study included 50 dogs, 30 diagnosed with CVL and 20 healthy controls. Dogs were classified based on their treatment regime (receiving resveratrol [200 mg/dog/d] and/or allopurinol [10 mg/kg/d]). Clinical scores, anti-leishmanial antibody titers, and routine blood parameters were evaluated on days 0, 15, and 30 after treatment. Results Dogs treated with the combination therapy showed the most substantial improvements, with a 75.77% reduction in clinical scores and an 81.25% reduction in antibody titers. Dogs receiving allopurinol exhibited a 56.25% reduction in clinical scores, while those treated with resveratrol showed a 42.82% reduction. Antibody titers were less significantly reduced in the resveratrol group (67.5%) compared to the allopurinol group (45%). Conclusions Combining resveratrol with allopurinol enhances the therapeutic efficacy for CVL, leading to significant clinical improvements and reductions in antibody titers. Resveratrol alone also demonstrates promising anti-leishmanial effects. Clinical Relevance Resveratrol, either alone or in combination with allopurinol, offers a feasible and effective alternative for improving clinical outcomes in CVL. Its incorporation into standard protocols may enhance treatment strategies in veterinary medicine
Analysis of Antibiotic Resistance Among KPC-3 Positive Pseudomonas aeruginosa Strains Isolated from Intensive Care Unit Patients
No full textThe increase in antibiotic resistance among microorganisms is an issue of utmost importance. Specifically, the resistance to carbapenem and extended-spectrum β-lactam antibiotics in gram-negative bacteria has had a considerable impact on patient morbidity and mortality rates. This research is centered around the examination of severe infections caused by Pseudomonas aeruginosa and KPC-3 in patients who have been admitted to intensive care units. P. aeruginosa strains were collected from the intensive care units of Van Training and Research Hospital. In order to conduct a thorough microbiological analysis of these strains, various methods including culture, biochemical tests, antibiogram tests, and polymerase chain reaction (PCR) were employed. A total of 126 carbapenem-resistant P. aeruginosa strains were extracted from patients within intensive care units. Among these isolates, 17 were identified as being both multidrug-resistant and positive for KPC-3. Interestingly, all of the identified strains exhibited sensitivity to Amikacin, Piperacillin, Piperacillin/Tazobactam, Gentamicin, Colistin, Fosfomycin and Tigecycline. The identification of KPC-3 positive P. aeruginosa strains among patients in our hospital highlights a concerning issue. It has been observed that this presence increases the risk of both mortality and morbidity in affected patients. Consequently, it has been deemed crucial to address this matter in terms of hospital surveillance practices and the implementation of strategies to combat antibiotic resistance
Sağlık Hizmetleri Sunumunda Çevreye Duyarlı Politikalar: Yeşil Hastaneler, Sürdürülebilir Uygulamalar ve Karbon Ayak İzini Düşürme Stratejileri
No full textEvaluation of Anatomical Measurements of the Bulbus Oculi by Optical Biometry in the Eastern Region of Türkiye
No full textBackground and Objectives: The objective was to assess ocular biometric measurements in relation to age and gender among patients scheduled for cataract surgery, utilizing an optical biometry device. Materials and Methods: The optical biometric parameters evaluated included axial length (AL), central corneal thickness (CCT), anterior chamber depth (AD), lens thickness (LT), horizontal corneal diameter (WTW), and keratometry values in the flat (K1) and steep (K2) meridians. Astigmatism (AST) was also measured as the difference between these keratometry values. Results: A total of 14,183 optical biometric measurements were included in the study. The average age of the participants was determined to be 56 (3–110) years. The average AL was 23.57 ± 1.45 mm, the average AD was 2.76 ± 0.42 mm, the average CCT was 518.13 ± 37.81 μm, the average WTW distance was 11.88 ± 0.59 mm, and the average LT was 4.19 ± 0.51 mm. The keratometry measurements were recorded as K1 = 43.39 ± 1.96 diopters (D), K2 = 44.51 ± 2.31 D, and AST = 1.12 ± 1.15 D. The average values for male eyes in terms of the AL, AD, WTW, and LT measurements were significantly higher than those for female eyes (p = 0.001). The average K1 and K2 values were flatter in males than in females, while the AST value was found to be higher in females (p = 0.001). As age progressed, the mean AL decreased, the CCT decreased, the AD narrowed, the WTW distance decreased, the LT increased, and the keratometric values K1, K2, and AST decreased until the age of 60, after which they increased again. Conclusions: Research indicates that the measurements of the bulbus oculi are generally larger in males than in females. Furthermore, each of the optical biometric measurements is interrelated. Over time, these measurements may change
