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Research regarding the qualitative assessment of the contamination with antibiotic residues from the milk sold in the agro-food markets and in the commercial network in Iași
No full textThe marketing of antibiotics directly to animal owners and the intensification in recent years of the use of these drugs
without the recommendation of a veterinarian has led to the increasingly frequent presence of antibiotic residues in milk
that is intended either for human consumption or for obtaining dairy products. The use by processors of milk with
antibiotic residues is difficult to achieve. According to European Union food safety legislation, milk harvested from
cows treated with antibiotics must be collected separately, and isolated. It cannot be given for processing or human
consumption. Applying thermal treatment, especially pasteurization, to milk with antibiotic residues above the
maximum allowed limit has insignificant effects, processors have been convinced of this over the years. The interest of
the study for the detection of antibiotic residues in milk is very current, even if the waiting period after treating the cows
with antibiotics is recommended, often this period is not respected, the consideration being the significant economic
losses. The rapid strip test method was done with strip tests AuroFlow, which are designed for the simultaneous
detection of beta-lactams and tetracyclines. The working samples were represented by processed milk sold in
supermarkets and raw milk sold in agro-food markets in Iasi. The distribution of processed milk samples was 1 positive
sample each in 2 of the supermarkets where the samples were collected, these being in the percentage of 4%. UHTtreated milk in all 5 supermarkets did not show any positive tests. On the other hand, the sale of raw milk in the agrifood markets showed a percentage of 20% positive samples, out of several 25 samples examined, the rate is much
higher compared to processed milk
A case of pericarditis in Angus cattle
Get PDFA 10-years-old Angus cow was evaluated on the field for suspected traumatic pericarditis. Physical exam findings
included tachycardia, mixed dyspnea, decreased intensity of heart sounds on the left, absence of heart sounds on the
right but presence of fluid sounds, bilateral turgidity of the jugular veins, positive venous compression test, brisket and
ventral abdominal oedema. The cow exhibited light pain while palpation at brisket region. Hematology revealed
significant erythrocytopenia, higher hematocrit, lower hemoglobin concentration, significant leukocytosis with
neutrophilia and lymphocytopenia. Serum biochemical findings had reduced glucose, urea nitrogen, albumin, sodium,
potassium, chloride, calcium, phosphorus levels and increased levels of AST, ALT, GGT, total protein and globulin.
Ultrasonography has shown pericardial effusion and strands of fibrin. The prognosis was poor, and pericardiocentesis or
pericardiotomy were inadequate methods of treatment, thus euthanasia was indicated
Anaesthetic management of canine patients with mitral valve regurgitation undergoing soft tissue surgery
Get PDFThe purpose of this study was to establish the anaesthetic management of canine patients with mitral valve regurgitation
that underwent soft tissue surgery and, also to understand the physiopathology of this cardiac disease and how
anaesthesia might influence the outcome of this patients.
The study was conducted on 30 canine patients aged between 9-14 years old belonging to different breeds represented
by mixed breed, Basset Hound, French Bulldog, Bichon and Pekinese
Increasing amino acids content of white wines with enzymes treatments
Get PDFWine’s chemical structure is affected by many biochemical transformations during the
winemaking process, which are catalysed by specific enzymes. These compounds participate in the
formation of amino acids, which also have fundamental functions in the sensory quality of wine.
Therefore, this research focuses on monitoring the effect of enzymes on amino acid concentration
during the fermentation of Feteasca regala and Sauvignon blanc wines. A total of 22 amino acids
were quantified using an ultra-high liquid chromatography system coupled with mass spectrometry
detection. Data indicated a major impact of the analysed variables (enzyme type and grape variety)
on wine’s characteristics. Considerable amounts of some essential amino acids, such as histidine,
isoleucine, phenylalanine, and tryptophan, were found in samples treated with pectinases preparations.
The administration of pectinases was more effective in the Feteasca regala wines in the applied
work conditions, although the -glycosides generated the highest values for most amino acids in the
Sauvignon blanc. Pectinases can provide more acceptable sensory characteristics of wine compared
to -glycosides in the applied work conditions (when they are applied in the pre-fermentation stage),
while these samples generally showed the lowest intensity for some negative descriptors, such as
phenolic, mineral or a bitter taste
Chitosan-mediated environment-friendly synthesis of gold nanoparticles with enhanced photonic reactivity
Get PDFWe developed a very simple, efficient and environment-friendly synthesis method for
the manufacturing of high-performance chitosan-capped gold nanoparticles that could be used for
biosensing applications. Gold nanoparticles were prepared through the spontaneous reduction of
chloroauric acid by chitosan, which was used as both a reducing and a stabilizing agent. The samples
were heated to a temperature of 60 C under ultrasonic conditions. The composite system made
of chitosan as a matrix and gold nanoparticles demonstrated a high stability in an aqueous buffer
solution. The nanoparticles displayed an enhancement in photonic performance compared with the
same property of individual components as a result of surface plasmon resonance at the interface
between the structural phases of the hybrid structure. The enhanced photonic reactivity of the hybrid
nanostructure may offer new insights for future possible biosensing applications
Energy value and chemical composition of some homemade dog food recipes
Get PDFGiven the fact that in recent years more and more pet owners have abandoned conventional, commercial diets in search
of more "natural" and "homemade" choices, we considered appropriate to evaluate 5 homemade diets for dogs
regarding chemical composition and energy value. Following the analyses carried out, the values for protein and fat
were within the minimum limit allowed by the Nutritional Guidelines for Complete and Complementary Food for Dogs
and Cats (FEDIAF) recommendations for maintenance. As for calcium and vitamin D levels, which were estimated
using a software - HYBRIMIN® Futter 5, the results were well below the recommended level, in the case of vitamin D,
values were even 0. The energy value was between 45 - 125 Kcal/100 g product. It is evident that the use of these
recipes in this study exposes animals to nutritional deficiencies, which shows how important it is to inform pet owners
of the risks involved in providing improperly prepared homemade diets
Influenţa produselor pe bază de GA4+7 asupra productivității plantației de măr din soiul Golden delicious
Get PDFThe experimental orchard was placed in the “Accesal Group” Ltd., founded in 2009. The study subject was Golden Delicious apple variety grafted on M 26. The trees were trained as slender spindles. The distance of plantation is 4.0 x 2.0 m. The studied gibberellic acid was GA4+7, product Gerlagib LG and Gibbera, SL. To study the influence of the treatment on fructification and quality of Golden Delicious apple variety, four variants were experimented: 1. Control, no treatment; 2. Gerlagib LG, 0.5 l/hа; Gibbera, SL, 0.25 l/hа; Gibbera, SL, 0.50 l/hа. In was demonstrated, that products Gerlagib LG and Gibbera, SL in dose 0.5 l/ha may be included in the technologic system to stimulate fruit formation, productivity and quality of fruits, up to 3 times, starting from petal fall stage and continuing intervals of 7-10 days
Morphological, physiological and quality performances of basil cultivars under different fertilization types
Get PDFThe prospect of replacing traditional chemical fertilization with organic and microorganismbased
fertilization meets the current demand for more sustainable cropping systems and healthy
food. In this respect, research was carried out to evaluate the effects of the factorial combination
between four basil cultivars (‘Aromat de Buzau’, ‘Macedon’, ‘Cuisoare’ and ‘Serafim’) and three types
of fertilization, namely chemical fertilization (with a solid chemical fertilizer), organic fertilization
(with chicken manure formulate) and microorganisms’ fertilization (with microorganisms formulate),
on basil yield, biochemical and physiological parameters and essential oil composition. The results
showed that the biometric parameters (plant height, number of stems and leaves and leaf area)
were significantly influenced by the cultivar; ‘Macedon’ obtained the highest values of plant height
(64.7 cm) and number of stems (20.33) and leaves (618.3) and ‘Serafim’ the largest leaf area (4901.7 cm2
per plant), while the type of fertilization did not affect these parameters. Regarding the biomass, the
influence of the cultivar was not significant on fresh biomass but was significant on dry biomass, with
‘Macedon’ showing the highest value (56.4 g plant1 dry biomass). The mentioned parameters were
significantly influenced by the type of fertilization, with the highest values recorded with chemical
fertilization. Both the cultivar and the fertilization type significantly influenced the physiological
parameters (the total content of assimilatory pigments and photosynthesis). Five phenolic compounds
were quantified from leaf extracts by HPLC-MS (caffeic acid, hyperoside, isoquercitrin, rutin and
quercitrin). Hyperoside was identified only in ‘Macedon’, while the rest of the compounds were
found in all the cultivars and varied depending on the cultivar and fertilization type. Regarding
the composition of the essential oil, variation was found depending on the cultivar and fertilization
type. In ‘Aromat de Buzau’, the main compounds were methyl chavicol and -linalool; in ‘Macedon’,
geranial and neral; and in ‘Cuisoare’ and ‘Serafim’, -linalool. Moreover, the PCA showed that the
‘Serafim’ cultivar has exclusive properties compared to the other cultivars. Our results highlight that
identifying the most effective interaction between genotype and fertilization type allows to optimize
yield and quality targets for sweet basil
The evaluation of the quality of the energy phytomass from industrial hemp Cannabis sativa grown in Moldova
No full textPlant biomass, phytomass, is an important source of renewable energy, and its importance has increased in regional and
national energy strategies. The goal of this research was to evaluate the quality of energy phytomass from industrial
hemp, Cannabis sativa, grown in the experimental plot of the National Botanical Garden (Institute), Chişinău, Republic
of Moldova, as feedstock for the production of biogas and solid bio-fuel. The analysis of the biochemical composition
indicated that the dry matter of the hemp plants harvested in the full flowering period contained 141 g/kg CP, 424 g/kg
CF, 457g/kg ADF, 704 g/kg NDF, 82 g/kg ADL, 247g/kg HC, 375 g/kg Cel, 88 g/kg ash, 506.7 g/kg carbon and 22.5
g/kg nitrogen, with biochemical methane potential 280 l/kg. The prepared hemp silage was characterized by specific
smell, 14.0 g/kg DM lactic acid, 7.7 g/kg DM acetic acid, 146 g/kg CP, 443 g/kg CF, 468 g/kg ADF, 716 g/kg NDF, 79
g/kg ADL, 248g/kg HC, 389 g/kg Cel, 134 g/kg ash, 481.0 g/kg carbon and 23.4 g/kg nitrogen with biochemical
methane potential 286 l/kg. The hemp solid bio-fuel, pellets, was characterized by high net calorific values (17.9
MJ/kg), specific density (1012 kg/m3
), durability (94%) and optimal ash content. Industrial hemp, Cannabis sativa, may
be use as multi-purpose feedstock for renewable energy production
Măceșul (Rosa canina) și păducelul (Crataegus monogyna) - surse horticole bogate în compuși bioactivi din flora spontană a României. Recenzie.
Get PDFRosehips (Rosa canina) and hawthorn (Crataegus monogyna) are considered important forest fruits from Romania's wild flora by Rosaceae family. According to research conducted on this topic, the horticultural sources above-mentioned are associated with many benefits for human health due to their richness in natural bioactive compounds. Increasing attention has been recently paid to the bioactive nutrients from rosehips and hawthorn. Rosa canina considered pseudo-fruit has been known to be an important medicinal plant since 2,000 years ago and includes several subspecies. Compounds of interest for human health included: flavonoids, carotenoids, fatty acids, high vitamin content (especially vitamin C), antioxidants and anti-inflammatory agents. As for their specific composition, it can be influenced by various climatic or technologic factors like all other plant products. It has been shown that several components of the rosehip can be used in medicine, especially due to its anti-inflammatory effect. Moreover, the consumption of this fruits may reduce symptoms associated with arthritic conditions) and also can be correlated with beneficial effects in the treatment of numerous diseases, such as: HIV infection, eye disorders (myopia, astigmatism), cardiovascular diseases (angina pectoris, arrhythmia, hypertension, myocardial injuries, etc). Furthermore, the plant of Crataegus monogyna has various interesting physiological and pharmacological activities due to the presence of different bioactive natural compounds. The most representative of phytochemicals from whole plant of Crataegus monogyna are represented by: flavan-3-ols (catechin, epicatechin), procyanidins (procyanidin B2, procyanidin B4, procyanidin B5), flavones and flavonols (hyperoside, vitexin, methoxykaempherol), anthocyanins and anthocyanidins (cyanidin-3-galactoside), chloregenic acids, triterpenes (oleanolic acid, ursolic acid, betulinic acid, butyrospermol, cycloartenol). The aim of this study was to make an inventory of the species, subspecies and varieties of hawthorn and rosehip, the health benefits provided by the use of these species and the ecological conditions that influence their growth and developmen