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Characterisation of the parasite load of river Bistrita tributaries, in Dornelor Basin, Romania
No full textDornelor Basin is
characterised by numerous high quality
water sources, which is proven by the fact
that the main mineral waters on the
Romanian market come from this area. The
study aimed to provide data on the
occurrence and human infective potential of
Giardia and Cryptosporidium, as the most
important water-borne parasites, from
Bistrița river tributaries of Dornelor basin,
North-Eastern Romania. Water samples were
collected from 10 tributaries of the Bistrita
river, from the level of sampling stations set
upstream and downstream from the
anthropic communities. The harvested water
samples were further processed using nonmolecular
methods in order to isolate
(oo)cysts. Subsequently, the isolated
Cryptosporidium and Giardia (oo)ccyst were
molecularly characterized through PCR and
genomic sequencing, which led to the
identification of Giardia in order to identify
them at species level. The outcomes revealed
the fact that the waters of the emissaries
under study have a low parasite load and
that, upstream from the human settlements,
the water is highly pure when related to the
protozoa under study. The increased load of
Giardia spp. and Cryptosporidium spp.
corresponded to important animal husbandry
activity. The obtained results underline a
potential public health risk
Study on the quality of homemade cheese in various research periods
Get PDFThe scientific research reflected in this study aimed at conducting microbiological scientific research on the microflora
of fresh homemade cottage cheese and in various freezing periods according to the scheme of classical laboratory
microbiological conduct.
The results of bacterioscopic microorganisms, Streptococcus microbial colonies, Lactobacillus, yeast cells and
conditionally pathogenic species were evaluated in this fresh dairy product and after some freezing intervals, which
recorded various microbiological values of a normal bacterial microflora.
These researches, which are characteristic to the safety of food microbiology, are of public interest and allow us to point
out that the homemade cheese-food product, often prepared from homemade milk raw material and often kept frozen
for certain reasons, is of good quality and meets the requirements. microbiological according to its quality
Study on the influence of climate, soil tillage, and some agricultural species on the process of water storage in the soil in the north-east Baragan area
No full textSoil and water are some of the prime components of nature. The development of life depends directly and indirectly on
the health and quality of the land. Regardless of its composition, the soil contains substances that allow the growth and
development of many plant species and microorganisms. The increase in temperatures, the decrease in rainfall, and the
applied agricultural technologies have contributed over the years to ruining the structure and components of the soil,
which negatively affected its humidity. The paper aims to identify the type of tillage in the dry-farming system and the
agricultural species cultivated, which can achieve optimal harvests without leaving the soil devoid of moisture. The
amount of water in the soil in the agricultural year 2019-2020, although it was deficient, exceeded the value of the
withering coefficient, thus allowing crops to grow. However, this was not enough to obtain rich harvests
Contributions to the optimization of the eco-conditionality system in direct payments for romanian farmers in the period 2021-2027
No full textThe paper proposes to conduct a study on the optimal use of the CAP's "eco-conditionality" instrument in the context of
the revision of the normative framework and procedures regarding the schemes and support measures for farmers in the
period 2015-2020 and the conditions applicable to them. Were also considered the significance of applying this
instrument as well as the issues that may arise from their non-application/improper application.
In terms of eligibility requirements, declared surface, payment rights, and surface control sanctions, the results of the
verification of surface payment requests for the years 2015 to 2021 reveal that a total of 7,800,417 payment requests
(applicants) were made for a declared area of 9,684,116 ha (annual average), with a total payment request value of
approximately €6,018,442.1 thousand. After the farmers' declared territories were verified, SAPS sanctions (unpaid
amount) in the amount of €372,122.8 thousand were put into effect. The primary role of agriculture, which accords this
industry a particular standing in EU regulations, is that of a supplier of agri-food goods for the general public's use. All
strategies for developing, supporting, and funding the CAP are built on this role. We put forth the following suggestions
in relation to the optimization of the cross-compliance mechanism within direct payments for farmers. To lessen the
obligations for small agricultural holdings where the risk is lower, the eco-conditionality requirements must be designed
in a way that takes into consideration the size of the agricultural holding. Farmers and their control bodies must be able
to understand the mandatory requirements for farmers and their control elements. It is advisable to eliminate GAEC
standards and SMR requirements that are difficult to audit and cannot be quantified. In any scenario, the quantity and
nature of cross-compliance requirements must be altered. Mandatory standards for farmers and the aspects of their
control must be pertinent and simple for farmers and control organizations to comprehend. It is advisable to eliminate
GAEC standards and SMR requirements that are difficult to audit and cannot be quantified. In any scenario, the
quantity and nature of cross-compliance requirements must be altered. Inspection quotas should be lowered if particular
control bodies have risk analysis systems that adhere to Community legal requirements and information on the extent of
compliance. Additionally, risk analysis models can be built for locations vulnerable to cross-compliance in order to
decrease the control sample
Aspects regarding the assurance of seed quality indicators in agricultural research units
No full textThe quality of products and services has become a determining factor of organizations' competitiveness. Making
progress in improving the quality of products and services must be a priority concern for the entire economy. Currently,
"quality management" is emerging more and more as a profitable solution for the development of organizations of any
kind. The production of seeds with excellent quality characteristics depends on the scientific basis of activities in the
field of seeds, including, on the one hand, the theoretical basis of genetics, seeds breeding, and on the other hand, the
activities of obtaining through in-depth understanding and the application of modern methods of conservative selection.
This knowledge is necessary for the permanent maintenance of the genetic structure and biological value at the initial
level of the genotypes of agricultural plants. In this sense, it is necessary to apply specific techniques for the production,
processing and preservation of seeds. In order to achieve these objectives, the accumulated knowledge must be
optimally applied to the entire process of seed production, starting with the selection of typical plants from varieties and
research in the field of conservative selection, obtaining it from the breeder's seed until ensuring the necessary certified
seed. Seed quality testing is carried out by seed testing laboratories that have been authorized for this purpose by the
competent authority. Official certification is achieved by issuing field inspection documents and laboratory analyzes
(tests) and by applying official labels to packages containing seeds that meet the quality conditions provided for in the
rules, by species or groups of species, or, as the case may be, of official vignettes and possibly seals
Co-infection with Babesia canis and Borrelia burgdorferi S.L. in a dog from Northeastern Romania: a case report
Get PDFThis study describes a clinical
case of a 9-year-old mixed-breed dog coinfected
with Babesia canis and Borrelia
burgdorferi. This dog was referred to a
private clinic in northeastern Romania for a
recurrent perianal tumour and a mild
inflammation in the right elbow. The dog
showed mild haemolytic anaemia, as well as
increased alkaline phosphatase and glucose
levels. Despite surgery and therapy, after
four days, the patient had developed
hyperthermia, severe anaemia and an
inflammatory syndrome. The blood smear
revealed the presence of piroplasm
organisms identified as ‘large’ Babesia spp.
On the 9th day of hospitalization the patient
died during the blood transfusion, before
applying the specific therapy for babesiosis.
The blood collected before blood transfusion
was tested for the following vector-borne
diseases: Babesia spp., Anaplasma spp.,
Ehrlichia spp., Hepatozoon spp. and
Borrelia spp. using molecular analysis. The
final outcome indicated a co-infection with
Babesia canis and Borrelia burgdorferi s.l.
In conclusion, the introduction of vectorborne
disease screening approach prior any
surgical procedure can prevent lifethreatening
events and improve diagnostic
accuracy in dogs infected/co-infected
simultaneously with different vector-borne
diseases
Clinical and paraclinical aspects in the acute diarrheal syndrome in dogs
Get PDFThe aim of this paper was to identify the clinical and paraclinical diagnostic elements of acute diarrheal syndrome in
dogs, useful in streamling of treatment. The clinical diagnosis in haemorrhagical acute diarrheal was easy to establish
given the profoundly altered general condition with cortical inhibition and melena; sometimes it has been clinically
evolve as dysentery (frequent bloody defecation). In catarrhal acute diarrheal, it can sometimes be profuse (exhausting),
accompanied by alteration of the patient's general condition and drowsiness. The ultrasonographical examination
revealed inflammation of the intestinal wall with a hyperechoic appearance and a halo exterior hypoechogenic
corresponding to parietal congestion. The haematological examination revealed hypochromic, normocytic anaemia and
a systemic inflammatory syndrome (increased WBC=17.2±0.3x10³/mm³, decreased RBC=5.3±0.4x106/mm³,
HGB=11.8±0.4 g/dl and CHEM=31.0±0.2 g/dl). The blood biochemical examination showed subclinical liver failure
without impairment of renal and exocrine pancreas functions. The sero-haemorrhagical acute diarrhea had clinically
manifested by cyclic episodes of diarrhea. The radiological examination revealed inflammation of the intestinal mucosa
and the presence of superficial ulcers, and the coproparasitological examination confirmed cryptosporidiosis. On the
other hands, the sero-haemorrhagical acute diarrhea was the consequence of traumatic gastroenteritis, confirmed by
radiological exam (dense, radiolucent contents in the gastrointestinal mass). In this situation, the haematological
examination revealed a systemic inflammatory process (increased WBC=18.0±0.3x10³/mm³) and hypochromic,
normocytic anemia (decreased CHEM=31.8±0.3 g/dl). The blood biochemical examination revealed subclinical liver
failure (increased ALT=88.2±0.3 IU/L and ALP=120.3±0.4 IU/L, values only)
Possibilities to improve the permanent grasslands of Dichanthium ischaemum (L.) Roberty from the moldavian forest steppe
No full textThe enhancement of permanent meadows involves conducting complex research, in order to know the limiting factors
in the optimal development of vegetation, specific improvement measures to be taken to increase production capacity,
feed quality and grazing capacity. The quantity and quality of the vegetation of the permanent meadows are largely
conditioned by the characters of the seasonal conditions, to which are added the anthropogenic influences through the
exploitation system. The objectives of this study were to highlight the influence of harvesting phenophase and
fertilization with mineral or organic fertilizers on the productivity and quality of the forage, with important implications
for obtaining high quality forage, depending on the type of fertilizer and the size of application doses. The experience
field was organized on a permanent grassland of Dichanthium ischaemum (L.) Roberty, in Andrieşeni locality, Iaşi
county, framed between the parallels 47°30'45.2”N and 27°15'42.0” E. The experimental factors were represented by
the harvesting phenophase, with three graduations: a1-harvesting at plants height of 15-18 cm, a2-harvesting at the ear
formation (control), a3-harvesting to full flowering and fertilization with seven graduations: b1- unfertilized (control),
b2- N50P50 kg/ha-1
annually, b3- N75P75 kg/ha-1
annually, b4- N100P100 kg/ha-1
annually, b5-10 t/ha-1
sheep manure annually,
b6-20 t/ha-1
annually and b7-30 t/ha-1
annually sheep manure applied at two years. From the results obtained on the
Dichanthium ischaemum meadow, it was found that its productivity is very low, and the level of dry matter production
can increase considerably depending on the harvesting phenophase and the fertilization used. The harvesting
phenophase and the applied fertilization had a marked influence on the quality of the fodder obtained from this type
derived from meadows, materialized by changes in the chemical composition with implications on the value of us
Results on the species Diabrotica virgifera virgifera le conte in the conditions of central Moldova
No full textThe species Diabrotica virgifera virgifera Le Conte (western corn rootworm) is part of the order Coleoptera, family
Chrysomelidae and is an invasive alien species native to Central or South America. In Europe, it was reported in 1992,
and in Romania in 1995. The attack produced by the species Diabrotica virgifera virgifera significantly reduces maize
production, the pest being a carrier of various pathogens that cause crop plant diseases (Bacal et al, 2020). In the present
paper, are presented partial results regarding the appearance, evolution and flight of adults of the species Diabrotica
virgifera virgifera, respectively, the attack that the insect produces in the maize crops in the Central area of Moldova. In
the year 2021, the flight of this species began in the first decade of July and continued until the third decade of
September. The maximum flight peak was achieved in the third decade of July, being 180 specimens/trap. In the year
2022, the flight of the species started in the first decade of July and continued until the end of September. The
maximum flight peak was recorded in the first decade of August, when a number of 182 specimens/trap were collected.
In the year 2022, a maize silk attack frequency of between 85% and 100% was recorded. From the results obtained in
2022, the treatment carried out on the vegetation with insecticides reduced the number of adults per plant from 1.6
specimens to 0.1 specimens
Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GFequina) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein Cows
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Open AccessArticle
Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GFequina) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein Cows
by Silviu-Ionuț Borş
1 [ORCID] , Dan-Lucian Dascălu
1, Alina Borş
2, Hossam M. Fahmy
3, Omaima M. Kandil
4 and Ahmed Sabry S. Abdoon
4,*
1
Research and Development Station for Cattle Breeding Dancu, Holboca, 707252 Iaşi, Romania
2
Department of Public Health, Faculty of Veterinary Medicine, University of Life Sciences “Ion Ionescu de la Brad” Iaşi, 700490 Iaşi, Romania
3
Clinical Laboratory and Blood Bank Department, Faculty of Medicine, Ain Shams University, Cairo 1181, Egypt
4
Department of Animal Reproduction and Artificial Insemination, Veterinary Research Institute, National Research Centre, Dokki, Cairo 12622, Egypt
*
Author to whom correspondence should be addressed.
Animals 2022, 12(19), 2618; https://doi.org/10.3390/ani12192618
Received: 26 August 2022 / Revised: 23 September 2022 / Accepted: 26 September 2022 / Published: 29 September 2022
(This article belongs to the Special Issue In Vitro Growth of Mammalian Follicles and Oocytes)
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Simple Summary
Lyophilized horse-platelet-derived growth factor (L-GFequina) is a brand-new, improved platelet-rich protein growth factor. It has been successfully used to treat a number of inflammatory and degenerative musculoskeletal diseases in several disciplines of regenerative medicine. Our study aimed to evaluate the possibility of using an intraovarian injection of L-GFequina in the assisted reproduction of Holstein cows. L-GFequina employs allogenic, pathogen-free platelets from horses that have been successfully used in other species to prevent changes in technical platelet specifications caused by the centrifugation process and time, as well as the integrity and quality of autologous platelets. The number of ovum pick-up (OPU) sessions per cycle, the growth of medium- and large-sized follicles, the number of COCs produced per cow per session, and the formation of blastocysts during OPU-IVEP in eCG stimulated Holstein cows were all boosted in this study by an intraovarian injection of L-GFequina.
Abstract
The purpose of this study was to determine the impact of intraovarian injections of a reconstituted lyophilized growth-promoting factor extracted from horse blood platelets (L-GFequina) on the number of ovarian follicles, the recovery of cumulus–oocyte complexes (COCs), and embryo development to the blastocyst stage in Holstein cows. Thus, 12 Holstein cows were assigned to three protocols. According to the number of punctured follicles in protocol 1, ovum pick-up (OPU) was conducted on days 6 and 14 of the cycle (day 0 = estrus). In protocol 2, every large follicle (more than 7 mm) was removed, and 1 mL of L-GFequina was intraovarian injected (day 0). Two days later, equine chorionic gonadotropin (eCG) was administered, and OPU sessions were conducted on days 6, 10, and 14. The same ovarian stimulation procedure as that in protocol 2 was performed in protocol 3, except that equine L-GFequina was not supplied. OPU was carried out on days 6 and 10 of the cycle. The results indicate that the intraovarian injection of L-GFequina significantly (p < 0.05) increased the number of OPU sessions per cycle, the recovery of cumulus–oocyte complexes (COCs), and the production of blastocysts. In conclusion, an intraovarian injection of L-GFequina can improves OPU-IVEP results in Holstein cows