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Motility parameters of equine epididymal spermatozoa after 24 hours intra-epididymal exposure to lidocaine using two commercial extenders
No full textEpididymal spermatozoa is the last source for gamete rescue in case of emergency castration or sudden
death of a valuable stallion, thus an ideal harvesting and preservation technique should be employed.
Routinely, 2% lidocaine intraparenchymatous administration is used to provide analgesia prior to
castration, but studies on the effect of lidocaine on epididymal spermatozoa motility parameters are limited.
The purpose of this study was to determine the effects of lidocaine on equine epididymal spermatozoa, after
24 hours intraepididymal cool storage using two commercial extenders. We hypothesized that
intraepididymal prolonged exposure to lidocaine, might affect motility parameters of epididymal stallion
spermatozoa and that different extenders might have an impact. Sperm was collected from 20 epididymides
of routinely castrated 3 year old KWPN stallions. 4 stallions received 10 ml 2% lidocaine
intraparenchymatous 10 minutes prior to castration and 6 stallions were not medicated. Testicles were
transported to an equipped facility and cooled stored for 24 hours. From each sample an aliquot was diluted
in a commercial egg yolk based extender, and another in a commercial extender containing defined milk
proteins. Motility parameters were registered 30 minutes after dilution, computer assisted. There were no
statistical differences between motility parameters of spermatozoa exposed to lidocaine and spermatozoa
not exposed, however progressive motility and linearity significantly differed among the two extenders
Comparison of IPM packages on flower thrips and pod borers management of mungbean with recommended practice
No full textEffectiveness of integrated management approaches using blue sticky trap, pheromone trap, bio and synthetic insecticides were evaluated against major insects, like flower thrips and pod borers of mungbean at Regional Agricultural Research Station, Rahmatpur, Barishal and Pulses Research Centre, Ishurdi,Pabna, Bangladesh, respectively, during
two consecutive years of 2018 and 2019. All of the management packages significantly reduced flower infestation, thrips population and pod borer infestation in mungbean. The highest percentage of reduction of flower infestation, thrips population and pod borer infestation was found in IPM package-3: installing blue sticky trap + two spraying of chlorfenapyr (Intrepid 10 EC) @ 1 ml/l + third spraying with (chlorantraniliprole + thiamethoxam), i.e. Virtako 40 WG) @ 0.15 g/l, followed by IPM package-1, IPM package-2 and recommended practice (spraying imidacloprid, i.e. Imitaf 20 SL @ 0.5 ml/l). The highest yield was also recorded from IPM package-3, which was statistically similar to IPM package-1, followed by IPM package-2 and recommended practice. Although the IPM
package-3 provided the highest yield and return, followed by IPM package-1, but
recommended practice (farmer’s practice) gave the highest benefit because of higher
cost of IPM components brought down the profit margin of IPM packages. The components of IPM package-1, i.e. biopesticides, are ecologically safer than that of IPM package-3 (synthetic chemical insecticides). So, considering environment friendliness, the IPM package-1: installation of blue sticky trap and pheromone trap + two spraying of
azadiractin (Biomeem plus 1EC) @ 1 ml/l + third spraying with spinosad (Success
2.5 EC) @ 1.2 ml/l would be the best package for controlling flower thrips and pod borers of mungbean with higher yield in the insects prone areas, without harming the ecosystem
Influence of refrigeration parameters on water-holding capacity of poultry meat
No full textImportance of technological properties derives from needing of provide of some exact information regarding further processing perspective of meat obtained by slaughtering, in conditions of economic efficiency aiming to optimize both product’s quality as well as the itself process. The study had as general aim the evaluation of three refrigeration regimes different by temperature and air current flow, analysing three anatomic cut regions (breast, upper thighs and lower thighs), to characterize the lost in weight through refrigeration. The values of primary statistical estimators attributed to data which characterized the dispersion degree of the results obtained for weight losses by refrigeration at poultry meat were quite reduced at musculature of the samples from all experimental batches. So, the mean standard error was between limits 0.012–0.052 and calculation of variation coefficient leads to obtaining of values into interval 7.59–24.32%, describing a high homogeneity of character at pectoral musculature and medium to low in case of muscular samples from thighs level for all those three experimental batches, the lack of homogeneity inside experimental batch being attributed to individuality of biological material
Modelling of Health Risk Associated with the Intake of Pesticides from Romanian Fruits and Vegetables
No full textThis study is focused on the assessment of risks caused by pesticide residues to Romanian and other European populations, by modelling the acute and chronic risks considering short- and long-term exposures to pesticide residues in specific fruits and vegetables from different Romanian regions. Data were obtained from the Romanian 2016 official monitoring programme. For assessing the dietary risk, we used the Pesticide Residue Intake model—PRIMo. According to the official data, it was found that 50.44% of fruit samples and 28.25% of vegetable samples were contaminated with pesticides. Our study focused on acute risks and chronic risks (in a maximalist worst-case scenario) posed by pesticide residues in strawberries, apples, lettuce and potatoes, given both their high degree of consumption and contamination with pesticides. The short-term exposure assessment of children’s health due to consumption of apples, lettuce and potatoes contaminated with dimethoate, chlorothalonil and carbendazim, revealed exposure levels higher than the acute reference dose (ARfD, as 100%), raising acute risks. On the other hand, the long-term exposure assessment showed that the highest percentage from the acceptable daily intake (ADI, as 100%) was obtained for German children (DE child) (273.9%), followed by Netherlands children (NL child) (143.7%) diets, based on consumption of apples with dimethoate residues. Therefore, serious measures are needed for banning pesticides such as dimethoate, chlorothalonil and carbendazim from all countries in the EU. This would reduce the health risks generated by the consumption of contaminated fruits and vegetables
Research on structural and functional status of supply channels in irrigation systems
No full textIrrigation systems in Romania were built 40-60 years ago, and those that are still in operation have varying degrees of
infrastructure degradation. The degradation of the structural components of the irrigation system determined the
appearance of water losses and the decrease of the exploitation efficiency. Research conducted over about 15 years has
shown the state of advanced degradation of the supply channels and a significant decrease in their operating yields up to
40-60%. The absence of maintenance, repair and rehabilitation works determined the structural degradation of the canals.
The degradation phenomena are represented by clogging, compaction, erosion of the slab sealing joint, cracking - breaking
- movement of the slabs on the perimeter of the canal, etc. Important degradations are highlighted in the constructions on
the canals: hydrotechnical derivation nodes, underpasses of roads, water intake of the pumping stations, etc. All this causes
a large part of the volume of water transported by the canals to be lost through infiltration. The current structural and
functional state of the irrigation canals requires the immediate application of rehabilitation works
Obtained results regarding the optimization of grain sorghum technology in pedoclimatic conditions from Central of Moldova
No full textIn recent decades in Romania and in many other regions of the world, there is a high frequency of drought years, to three years out of every five. This climate negatively affects the productivity of agricultural crops, and agricultural research must find solutions to diversify the assortment of crops that in tougher environmental conditions guarantee the
stability and profitability of agriculture. One of the safest solutions is the cultivation of sorghum, whether for grain or biomass, which due to good drought resistance, the ability to withstand high temperatures and capitalize on poorly fertile land is superior by the yields of all crops and especially, cereals and fodder plants. In the pedoclimatic conditions
from the Center of Moldova, starting with 2018 year, were initiated some researches regarding the optimization of the cultivation technology for grain sorghum, in order to create a technology in relation to the new climatic conditions and in relation to the protection of the environment. The results obtained on average over two agricultural years, 2018 and 2019, indicated a very large variation of grain production depending on the sowing density provided and on the applied mineral fertilizers, this being between 4074 kg/ha and 10234 kg/ha. The best results were obtained in the variants sown
with 30 g.g./sqm and fertilized with N150P80 with and without the application of the biostimulator Aminosol. From the point of view of economic efficiency, the variant sown with 30 g.g./sqm and fertilized with N150P80 was the most profitable
Studiul expresiei temporale și spaţiale a purin permeazei Pup1 în Medicago truncatula, utilizând hibridizarea in situ ARN-ARN
Get PDFMolecular nitrogen (N2) is the most abundant element in the
atmosphere, but not in a form that can be directly assimilated by the
plants. Thus, nitrogen is a significant limiting factor for plant
development and productivity. The symbiotic interaction between
legumes and rhizobia, resulting to the reduction of the atmospheric
nitrogen into ammonia, has been considered one of the linchpins of
sustainable agriculture for many centuries. There is substantial
evidence, for example, data bioinformatics analysis and functional
genomics that in certain types of symbiotic nitrogen fixation, rhizobia
need nucleotide bases, particularly purines. The study of the role of
purines and their transport system from the host plant in rhizobium will
result in a better understanding of the molecular and biochemical
mechanisms underlining symbiotic nitrogen fixation and improve our
ability for the genetic manipulation of this beneficial interaction. In the
present study, an in situ RNA-RNA hybridization approach was
performed in order to determine the temporal and spatial expression of
purine permease Pup1 gene during the different stages of Medicago
truncatula nodule development. In situ RNA-RNA hybridization revealed
a strong expression of the purine permease Pup1 gene during nodule
development. Specifically, purine permease Pup1 gene transcripts
shared a similar spatial expression pattern in nodules at all
developmental stages examined and were localized in the central tissue,
the vascular bundles and the nodule parenchyma in nodules at 14, 21
and 28 days post inoculation
Modalităţi de valorificare a teraselor spaţiilor de locuit din mediu urban
Get PDFThe concept of terrace or green roof, arose from the desire to bring
nature as close as possible. Thanks to the increasingly advanced technologies of
recent years, we can achieve this goal even when we live in urban areas. The
benefits of this work could be seen in the proposal of a design solution that aimed
to reduce energy consumption and noise pollution, 60% rainwater retention and
last but not least reduce the temperature of the roof by up to 7 degrees in the warm
season and increase it by up to 5 degrees in the cold season. Thus, in this work, the
suitability of some dendrological species for an arrangement located on a terrace
located on the last level of a block of flats in the Tudor Vladimirescu neighborhood
was analyzed. In carrying out this work, the choice of planting material was taken
into account to ensure the decoration in a wide range
Considering soil water content, nutrients movement, phenology and plant growth with reference to development of functional foods in a lysimeter study
No full textLysimeter is equipped with mechanisms for weighing by load cells enable automated measurements, and the signals resulting from weight changes in the system due to evaporation that are generally recorded in a data acquisition system. According to methods of measuring water content, lysimeters may be divided into weighing lysimeter and non-weighing lysimeter. The weighing lysimeters provide scientists the basic
information for research related to evapotranspiration, and they are commonly divided into two types, continuous weighing and intermittent weighing. Weighing lysimeters have been
used to quantify precipitation (P) not only in the form of rain or snow, but also dew, fog and rime, and also to determine actual evapotranspiration (ET). Compared to laboratory experiments, out-door lysimeter studies have advantages, like being closer to field environment conditions, it is possible to grow plants and therefore to study the fate of chemicals in soil/plant systems, transformations and leaching. The limitations are costy, which depend on design, variable experimental conditions, such as environmental/
climatic parameters, which are normally not controlled, the soil spatial variability is normally less, they are not suitable for every plant species and even every soil type. The objective of lysimeter is defining the crop coefficient (Kc), which used to convert ETr into equivalent crop evapotranpiration (ETc) values, and determing agronomical characteristics of crops, which are planted in the field of lysimeter. The duration of a lysimeter study is determined by the objective of the study, but for different crops, it should normally be at least two years. Weighing lysimeters using load cells have the advantage of measuring the water balance in the soil over a short time and with good accuracy. Precipitation should be recorded daily at the lysimeter site. All weather data like air temperature, solar
radiation, humidity and potential evporation should be obtained onsite, and the frequency and time of measurements should be at least daily
Studiu privind creşterile vegetative în şcoala de viţe la soiurile pentru struguri de vin 'Aromat de Iaşi' şi 'Golia', prin analiza pigmenţilor fotosintetici foliari
No full textThe physiological role of the assimilating pigments is complex, being involved in the processes of oxidation-reduction, in the photosynthesis and protection processes against ultraviolet radiation. The study aims to track vegetative growth during the vegetation period by spectrophotometrically determining the content of chlorophylls (a and b) and carotenoids from the leaves of vine varieties (Vitisvinifera L.) for wine grapes Aromat de Iaşi and Golia, new varieties created within the Research and Development Station for Viticulture and Winemaking Iasi. The two varieties were grafted on three rootstocks (RipariaGloire, Berlandieri x Riparia Selection Oppenheim 4-clone 4 and Berlandieri x Riparia Selection Crăciunel 2), thus creating six working variants. Following the completion of the observations, Golia/SO4-4variant recorded the best results in terms of vegetative growth in the vegetation period measuring 41.83 cm (before wood maturation)followed very close by Aromatde Iasi/RipariaGloire, which recorded 41,71 cm at the last measurement. Regarding the determination of the content of photosynthetic pigments (chlorophyll a and b, respectively carotenoids), the best results were obtained in the Aromat de Iaşi variety on almost all variants. Golia grape variety registered smallervalues