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Efectele câmpurilor magnetice de frecvenţe foarte joasă asupra germinării seminţelor de tomate
In this paper were investigated the effects of magnetic fields on the germination of tomato seeds, Korall cultivar. The experiments were performed under laboratory-controlled conditions to eliminate the influence of variations in environmental factors. The tomato seeds, during the whole germination period, were exposed to a magnetic field generated by electrical conductors crossed by different electric currents. Three types of extremely low frequency magnetic fields were used for treatments (0 Hz - 13 μT; 0 Hz - 20 μT and 50 Hz - 20μT). The results obtained are useful for the development of a smart system for stimulating the germination of tomato seeds
Pestivirus spillover effect: molecular detection of bovine viral diarrhea virus in domestic and feral pigs
Pestivirus infections are important in the livestock industries, with infection occurring in cattle, sheep and pigs. The Pestivirus genus of the family Flaviviridae, includes four recognized species: bovine viral diarrhea virus 1 (BVDV-1), bovine viral diarrhea virus 2 (BVDV-2), border disease virus (BDV), and classical swine fever virus (CSFV). All pestivirus species can infect pigs, therefore accurate and specific pestivirus detection and differentiation is of great importance to assure control measures in swine populations. The aim of the study was the molecular detection of different pestiviruses in domestic and feral pigs. A total of 527 samples (92 pigs and 435 wild boars) were tested for pestiviruses detection using molecular assays. Eleven positive samples (6 wild boars and 5 domestic pigs) were identified using panpestivirus primers targeting the 5’- UTR region of the pestivirus RNA genome. Further all the positive samples were sequentially tested for detection of CSFV, BVDV-1 and BVDV-2 using specific primers. All RNAs were identified as positives for BVDV-1 and no amplification signals were obtained from BVDV-2 and CSFV. The current detection of BVDV-1 in clinical swine specimens highlights the important risk factor of swine population as reservoir and consequently carrier for BVDV
Cercetări privind influenţa unor secvenţe tehnologice asupra producţiei de tuberculi şi biomasă la topinambur în condiţiile de la S.C.D.A. Secuieni
The paper aims to highlight the influence of the distance between tubers in a row and the nutrition system of the Jerusalem artichoke plant on the yields of tubers and biomass. The research was conducted between 2018 and 2019, in the experimental field of A.R.D.S. Secuieni. The obtained results showed that the studied factors influenced to a great extent the tuber yield obtained. Thus, the yield of tubers varied within wide limits, being between 23.16 t / ha (40 cm x N0P0K0) and 32.77 t / ha (50 cm x N120P120K80). The interaction of the studied factors influenced the yield of biomass at Jerusalem artichoke, ranging from 25.15 t / ha (40 cm x N0P0K0) to 35.45 t / ha (50 cm x N120P120K80)
Investigations regarding on the clinical expression of Bluetongue virus infection of domestic ruminants in the South-East region of Romania
Bluethonge has a significant economic impact, mainly due to the effect of the disease on animals (morbidity, mortality, reproductive insufficiency, reducced of milk production and animal weakening) and, in particular, disruption of international trade in animals and animal products. In the South-East region of Romania, in the period 2014-2017, animals with clinical signs specific to Bluetongue virus were identified.1437 domestic ruminants with a suspected disease were examined. Of these, 418 (29,08%) had clinical features similar to those of Bluetongue. The incidence of clinical signs in animals with suspected disease varied by species. Thus, the clinical aspects were present in 22,21% cattle,
68,21% sheep and 1,54% goats. Following clinical examination, the simultaneous presence of several clinical signs specific for Bluetongue virus infection was observed in cattle and sheep. In cattle, the incidence of clinical signs was variable, most frequently reported conjunctival mucosal hyperemia, epiphora, sialorrhea, gingival ulceration, swelling and cyanosis of the tongue, nasal edema with ulcerations of the nasal mucosa and muco-purulent jet, circumcised necrosis at the level of the udder, accentuated weakening, congestion of the coronary area, torticollis, digestive disorders, exongulations and inability to move. The pathogenesis of Bluetongue is similar in cattle and sheep, so that the clinical evolution was similar in the two species. In goats, the disease evolved inapparently or with diminished clinical expressions: prostate condition, mucopurulent discharge, edema and nasal ulcers, cyanosis of the mucous membranes, curls and limping. In addition to the immediate losses, the onset of the disease can generate long-term effects, which can decisively affect the Romanian animal breeding sector. The ease with which the Bluetongue disease has spread, shows that the territory of Romania is affected by climate change caused by global warming, which allowed the transmitting
vectors to proliferate and spread the Bluetongue virus to the receptive animals
Sustainable biomass pellets production using vineyard wastes
Vineyards waste has a great importance as biomass, a renewable source of energy. In this paper eight vine shoot varieties were used for the production of pellets by densification of feedstock materials with four ranges of moisture contents (6–8%, 8–10%, 10–12% and 12–15%). A moisture content of 10% gave durability higher than 97.5% and a calorific value greater than 17 MJ kg−1 and the small durability was obtained for 6–8% moisture. The study shows the significant influence of water during densification. The physicochemical and energetic properties of pellets were evaluated in accordance with ISO 17225-6 (2014). The obtained pellets were also structurally characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). SEM analysis showed the formation of carbon microsphere after pelletization, due to the increase of bulk density and durability of pellets. Also, XRD analysis revealed the crystallinity of cellulose, while TGA analysis showed a total decomposition of pellets. The obtained pellets were burned in a domestic boiler and the flue gases were measured. The preliminary results showed that the vineyard residues had higher emissions, but below the admitted limits, with the exception of carbon monoxide content. The obtained results suggested that the biomass wastes can be used for the production of pellets, aiming to enhance the research for the manufacturing of these sustainable biofuels with some remarks regarding risk of corrosion and slag formation during prolonged use
Evaluation of the production and quality potential of Muscat Ottonel and Cabernet Sauvignon varieties in relation to climatic factors in Dealu Mare Vineyard
The varieties taken into study were Muscat Ottonel and Cabernet Sauvignon, part of the basic assortment for the
production of quality wines, typical for Dealu Mare vineyard, which are very valuable in view of the oenological aspect.
The experimental results obtained showed that under the ecoclimatic conditions specific to 2019 year, characterized by
a high heliothermic regime, on the background of low water resources, especially during the veraison-ripening period of
the grapes when the ripeness of the grapes was slow. Under conditions of water stress (precipitation reduced by 40.8
mm compared to the normal value of 124.9 mm), the growth rate of the berry weight being 1.28 g/day (Muscat Ottonel)
and 0.93 g/ day (Cabernet Sauvignon) and sugar accumulation of 1.59 g/l/day (Muscat Ottonel) and 1.00 g/l/day
(Cabernet Sauvignon). The total acidity of the must had very low values, 5.8 g/l tartaric acid in the Muscat Ottonel
variety and 6.5 g/l in the Cabernet Sauvignon variety, which resulted in a large increase of the glucoacidimetric index,
far exceeding the optimal value required for the production of wines with a high degree of quality and typicality
Studiul privind influenţa provenienţei bulbilor-plantă mamă din verigi de selecţie diferite, precum şi a mărimii bulbilor-plante mamă, asupra dezvoltării vegetative şi generative a mugurilor în cîmpul de seminceri la linia de ceapă trienală “Diana”
The work includes partial results of some research carried out over a period of time which includes several biological cycles ,,from seed to seed" on the determinism of some quantitative features of mother-plant bulbs, to ,,Diana" onion of chives variety, on the vegetative and generative development of plants from seed-tree phase. The quantitative characteristics are mainly associated with the size of the bulb (the longitudinal diameter, transversal diameter, bulb weight, number of developed buds inside bulb, etc.)
Study regarding the influence of :breed, sex ant tests on the place at the world champioships for young dressage horses in the year 2018
At the World Championship for young dressage horses, year 2018, for the age of 5 years, participated 13 different breeds, belonging to traditional breeds and sport breeds.
For this research we examine all 5 year old dressage horses from Euro dressage World
Championships for young dressage horses year 2018, divided in 2 groups for breed variable (traditional breeds and sport breeds), and 2 groups for sex, and test variables.
Data was manipulated using Excel 2007 (Microsoft) and was analyzed using SPSS Version 21 for Windows (IBM, USA). The aim of the statistical analysis was to explore any differences in the results at the World Championships for Young Dressage Horses in the year 2018 between horses from different breed, sex and test. The value of alpha was set at 0.05 for all statistical tests. In preliminary test and in consolation test there were no recorded differences between the sexes of the participating horses. Instead, if in the preliminary test and in the consolation test participate both: horses belonging to
traditional breeds and horses belonging to sports breeds, in the final test only qualifies horses belonging to sports breeds. The horses results at the World Championships for Young Dressage Horses in the year 2018 at final test is significantly higher (p = 0.042) in the case of stalions ( =8.7250) compared to geldings ( =4.5800).
In conclusion we can say that the higher share of participation in the World Championship for young dressage horses and the qualification of the final tests belongs to the horses of the sports breeds
Acute idiopathic polyradiculoneuritis with secondary arterial hypertension in a 5-year-old male Siberian Husky
Acute canine idiopathic polyradiculoneuritis (ACIP) is one of the most common generalised neuromuscular diseases affecting dogs. In this report, we describe a 5-year-old, 25-kg, male, intact, Siberian Husky dog with ACIP with secondary induced arterial hypertension fsystolic blood pressure [mean (m) ± standard deviation (sd)], 214 ± 19mmHg; mean blood pressure (m ± sd), 164 ± 6.36mmHg; and diastolic blood pressure (m ± sd), 137 ± 0.7mmHgg and sinus tachycardia. Heart rate variability analysis indicated decreased vagal activity (low root-meansquare values of successive RR interval differences and percentages of the RR intervals differing by more than 50ms in the entire recording) and predominance of sympathetic activity.
Arterial hypertension was treated with amlodipine but remained greater than the upper limit for 51 days until the dog recovered ambulation. This is the first case report of ACIP and secondary arterial hypertension in a dog. Routine blood pressure measurements should be included in the monitoring of patients with ACIP if arterial hypertension might interfere with patient prognosis
Prenylated phenolics as promising candidates for combination antibacterial therapy: morusin and kuwanon G
Combination of antibiotics with natural products is a promising strategy for potentiating antibiotic activity and overcoming antibiotic resistance. The purpose of the present study was to investigate whether morusin and kuwanon G, prenylated phenolics in Morus species, have the ability to enhance antibiotic activity and reverse antibiotic resistance in Staphylococcus aureus and Staphylococcus epidermidis. Commonly used antibiotics (oxacillin, erythromycin, gentamicin, ciprofloxacin, tetracycline, clindamycin) were selected for the combination studies. Checkerboard and time-kill assays were used to investigate potential bacteriostatic and bactericidal synergistic interactions, respectively between morusin or kuwanon G and antibiotics. According to both fractional inhibitory concentration index and response surface models, twenty combinations (14 morusin-antibiotic combinations, six kuwanon G-antibiotic combinations) displaying bacteriostatic synergy were identified, with 4–512-fold reduction in the minimum inhibitory concentration values of antibiotics in combination. Both morusin and kuwanon G reversed oxacillin resistance of methicillin-resistant Staphylococcus aureus. In addition, morusin reversed tetracycline resistance of Staphylococcus epidermidis. At half of the minimum inhibitory concentrations, combinations of morusin with oxacillin or gentamicin showed bactericidal synergy against methicillin-resistant Staphylococcus aureus. Fluorescence and differential interference contrast microscopy and scanning electron microscopy showed an increase in the membrane permeability and massive leakage of cellular content in methicillin-resistant Staphylococcus aureus exposed to morusin or kuwanon G. Overall, our findings strongly indicate that both prenylated compounds are good candidates for the development of novel antibacterial combination therapies