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Occurrence of Babesia Species and Co-Infection with Hepatozoon canis in Symptomatic Dogs and in Their Ticks in Eastern Romania
No full textAlthough the distribution of Babesia spp. and Hepatozoon canis is well known in Romania, there is still a marked lack of information in many places of the country. This study aimed to investigate the occurrence of these haemoparasites in symptomatic dogs and in their ticks in Iasi, eastern Romania. Ninety owned dogs were subjected to clinical examination at the Faculty of Veterinary Medicine of Iasi and all detectable ticks (58 ticks from 15 dogs) were collected. Additionally, 124 ticks collected from the coat of other dogs (no. = 23) were included. Three Babesia species were found in dogs: Babesia canis (94.4%), Babesia vogeli (3.3%), and Babesia rossi (2.2%). All the dogs resulted negative for H. canis. The ticks were identified as follows: Ixodes ricinus (64%), Dermacentor reticulatus (33%), and Rhipicephalus sanguineus group (3%). B. canis (Minimum Infection Rate; MIR = 81%), B. vogeli (MIR = 3%), and Babesia microti-like piroplasm (MIR = 1%) were found in ticks. Moreover, 15 ticks were positive for H. canis, 6 were co-infected with B. canis, and 1 with B. microti-like piroplasm. This is the first molecular identification of B. rossi in two symptomatic dogs from Romania, although further studies are needed to investigate the vector competence of other ticks from Europe
Cervical oesophagotomy in a cat for foreign body removal - case report
Get PDFOesophageal foreign bodies
are a significant cause of morbidity and
mortality in small animals, especially in
carnivores. Due to the possibility of
complications such as perforation or
tracheal compression, the patient may
present an upper airway obstruction, which
might become a medical emergency. Here,
we describe a rare case of a large cervical
foreign object in a cat and review the
diagnostic and therapeutic approach of this
condition. A 4-year-old female cat was
referred to our clinic with signs of
dyspnoea, dysphagia and regurgitation.
The history and clinical exam suggested an
oesophageal foreign body, subsequently
radiographically confirmed. Because its
shape and position did not allow
endoscopic extraction, the foreign body
was removed via ventral cervical
oesophagostomy. Due to its location and
large size, it was necessary to fragment the
foreign body into two pieces for complete
extraction without injuring the
oesophageal walls. The patient had no
postoperative complications and was
discharged 7 days after surgery. In this
condition, an early diagnosis, followed by
an immediate surgical repair and a
rigorous postoperative care, correlates
with patient recovery and survival, being
crucial in reducing the high morbidity and
mortality rates that are usually associated
Application of novel microorganism-based formulations as alternative to the use of iron chelates in strawberry cultivation
No full textThe strawberry is a low-growing, herbaceous perennial plant, sensitive to iron deficiency.
The iron deficiency represents a nutritional disorder, leading to a decreased content of photosynthetic
pigments, which determines the yellow color characteristic of chlorotic leaves. Therefore, in
calcareous soils, the use of synthetic iron chelate is often mandatory in strawberry cultivation. The
employment of novel microorganism-based formulations as alternatives to the use of iron chelates,
was evaluated during strawberry cultivation by monitoring the morpho-biometric parameters,
chlorophylls, the iron content in leaves and roots, and the Fe chelate reductase activity involved in
absorption of iron during the chlorosis event in plants using the strategy I. The experimental design
envisaged growing strawberry seedlings on an inert substrate (pumice), irrigated with Hoagland
solution iron-free, with a 12 h photoperiod. After 42 days, at the first appearance of chlorosis symptoms,
plants were transplanted into a calcareous soil, and after seven days, they were treated, by a
single application, with a microorganism-based formulations (MBF), an inoculum (In) of Trichoderma
spp. and Streptomyces spp., or Sequestrene (Sq). Strawberry plants were sampled and analyzed
at 5, 10, 15, and 20 days from the treatments. The results showed that microorganism-based
formulations positively affected the strawberry seedlings, by reducing the chlorosis symptoms, producing
comparable effects to the Sequestrene treatment
Assessment of Andean lupin (Lupinus mutabilis) genotypes for improved frost tolerance
No full textSpring frost poses a challenge for all major crops and, in the case of Lupinus mutabilis
(Andean lupin) can cause severe damage or even total loss of the crop. Within the LIBBIO project
consortium, we conducted a series of experiments in order to develop a suitable protocol for screening
lupin germplasm under frost-simulation conditions. Four lupin accessions, one Lupinus albus and
three Andean lupins were used in the experiments (L. albus Mihai, L. mutabilis LIB 220, LIB 221, LIB
222). Seedlings at four developmental stages were challenged with five different levels of ‘frost’ stress
from low (2 C) to high (10 C). Notably, young seedling (cotyledons just breaking through the
soil surface) showed little evidence of frost damage for temperatures down to 6 C. At 8 C,
however, damage was evident, suggesting a cold tolerance threshold occurs at this temperature.
Interestingly, for later developmental stages, when the first and second leaves were visible, notable
differences were observed starting at 6 C. The results indicate that the plant growth stage is
an important parameter when screening for frost tolerance in germplasm. Overall, by identifying
Andean lupin genotypes adapted to high abiotic stress factors, farmers will be able to use it as
a reference crop with potentially a commercial interest from the food sector, or cosmetics, and
biofuel industries
Behavior of some romanian wheat varieties in A.R.D.S. Secuieni pedoclimatic conditions, during 2019-2021
Get PDFThis paper includes the results obtained after testing in multiannual comparative crops of a twelve national varieties of
winter wheat, thus aiming to introduce into the crop genotypes that have high adaptability to pedo–climatic conditions
specific to Central Moldova and thus a stability of production. These tests were performed at Agricultural Research –
Development Station Secuieni, and the material used in the field experience came from National Agricultural Reasearch
and Development Institute Fundulea and Agricultural Research – Development Station Turda The studied genotypes
showed the following variation of the average production: 4109 kg/ha (2019) – 3522 kg/ha (2020) – 8711 kg/ha (2021).
The low productivity in the first two years of experimentation is the result of unfavorable climatic conditions for winter
wheat cultivation characterized in the first agricultural year (2018 – 2019) by a dry autumn and in the second
agricultural year (2019 – 2020) by a dry spring. The Semnal variety presented the highest average productivity (6501
kg/ha) and a notable adaptability to unfavorable environmental conditions, which is why we recommend it to be
introduced in the zonal culture
Cutaneous papilloma in cattle
Get PDFBovine papilloma virus (BPV) -2 is the most common on haired skin. A 4-year-old Fleckviech Simmental cattle,
weighing 650 kg, with the presence of a formation on the upper eyelid of the right eye occupying approximately one
third of it, was examined. The lesion was nodular, hyperkeratotic, cauliflower-like growths. Hematological
abnormalities were lymphocytopenia, neutrophilia which indicates inflammation, infection, and hemoconcentration
which indicates mild dehydration. Changes in liver enzymes combined with low blood sugar and ketone bodies in urine
indicated ketosis status. Histological examination showed a hyperplastic epithelium covering an ordered proliferation of
mesenchymal cells. Numerous large cells with blue-gray cytoplasm were visible within the hyperplastic epithelium.
Corroborating the data of the clinical and paraclinical examinations, the diagnosis of cutaneous fibropapilloma was
made
Effects of different stages of maturity and postharvest treatments on the extension of shelf life and quality of banana
No full textAn experiment was carried out in the Laboratories of the Departments of Horticulture and Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh,
during the period from 23rd April to 10th May 2015. The two factors experiment was conducted for extension of shelf life and quality of banana under different postharvest treatments. The first factor was stages of maturity which had three maturity stages, viz.: 1) hard green (S1), 2) pale green (S2) and optimum maturity stage (S3). The second factor was postharvest treatments, which had five treatments, viz.: 1) control (room temperature), 2) keeping fruits in perforated plastic bag, 3) keeping fruits in perforated plastic bag containing KMnO4, 4) fruits treated with hot water for 5 min at 50°C and then kept in plastic bag containing KMnO4, and 5) fruits precooling for 30 min at 5°C and then kept in plastic bag containing KMnO4. The pulp to peel ratio, total soluble solids, total
sugar, reducing sugar, titratable acidity, were greater when fruits were harvested at
optimum maturity stage than hard green stage. Total soluble solids, total sugar, reducing sugar were increased with storage duration, but increasing trend was slower when fruits were pre-cooling at 5°C for 30 min and kept in plastic bag containing KMnO4. The longest shelf life of banana fruits (19 days) was observed when hard green stages fruits pre-cooling at 5°C for 30 min and kept in plastic bag containing KMnO4 and the minimum
shelf life (5.87 days) was observed in the combination of optimum maturity stage + control. So, it may be concluded that precooling for 30 min at 5°C and then kept in
plastic bag containing KMnO4 treatment should be used for extending shelf life and quality of banana
Dinamica zborului unor lepidoptere dăunătoare plantațiilor de viță de vie cu ajutorul sistemului Teraseya Fly
Get PDFAmong the practical applications of synthetic sexpheromones, the most important it is about constant supervision of insect populations for the purpose of forecasting and warning treatments or for their suitability.At present, in our country synthetic pheromones are produced for 33 species of insects of agricultural, horticultural and forestry importance. From their many, the AtraBot-type pheromone specific to the pest Lobesia botrana has been selected, which causes considerable damage to vineyards throughout the growing season. The present paper presents the results on flight dynamics and abundance of Lobesia botrana, a species harmful to red vineyards by means of the intelligent trap type Teraseya Fly.
AtraBot, Teraseya Fly, dinamic
Electrospun polyvinyl alcohol loaded with phytotherapeutic agents for wound healing applications
Get PDFIn this paper, hydroalcoholic solutions of Thymus vulgaris, Salvia officinalis folium, and Hyperici herba were used in combination with poly (vinyl alcohol) with the aim of developing novel poly (vinyl alcohol)-based nanofiber mats loaded with phytotherapeutic agents via the electrospinning technique. The chemical structure and morphology of the polymeric nanofibers were investigated using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). The addition of Thymus vulgaris, Salvia officinalis folium, and Hyperici herba extracts to the pure polyvinyl alcohol fibers led to changes in the morphology of the fibers and a reduction in the fibers’ diameter, from 0.1798 µm in the case of pure polyvinyl alcohol to 0.1672, 0.1425, and 0.1369 µm in the case of polyvinyl alcohol loaded with Thymus vulgaris, Salvia officinalis folium, and Hyperici herba, respectively. The adapted Folin–Ciocalteu (FC) method, which was used to determine the total phenolic contents, revealed that the samples of PVA–Hyperici herba and PVA–Thymus vulgaris had the highest phenol contents, at 13.25 μgGAE/mL and 12.66 μgGAE/mL, respectively. Dynamic water vapor measurements were used in order to investigate the moisture sorption and desorption behavior of the developed electrospun materials. The antimicrobial behavior of these products was also evaluated. Disk diffusion assay studies with Escherichia coli, Staphylococcus aureus, and Methicillin-resistant Staphylococcus aureus were conducted on the developed nanofibers in order to quantify their phytotherapeutic potential