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Contributions to the interpretation of microbiological research on the microflora of different fish varieties
The scientific research reflected in this study aimed to identify the bacterial microflora in different varieties of fish of
different marketing categories through microbiological investigation. The microbiological conditions for assessing the
examined fish assortments determined the presence of saprophytic germs, asserting a normal microflora according to the
requirements of the microbiological investigation standards and the identification of existing microbial species
Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine
The aim of the study was to establish the complete microbiological profile of boar semen (Sus scrofa domesticus) and to choose the most effective antiseptic measures in order to control and optimize AI reproduction in pig farms. One hundred and one semen samples were collected and analyzed from several pig farms. The microbiological profile of ejaculates was determined by evaluating the degree of contamination of fresh semen and after dilution with specific extenders. The bacterial and fungal load of fresh boar semen recorded an average value of 82.41/0.149 × 103 CFU/mL, while after diluting the ejaculates the contamination value was 0.354/0.140 × 103 CFU/mL. Twenty-four germs (15 bacterial and 9 fungal species) were isolated, the most common being Candida parapsilosis/sake (92%) and Escherichia coli (81.2%). Modification of the sperm collection protocol (HPBC) reduced contamination in raw sperm by 49.85% in bacteria (significant (p 0.05)
A synthetic profile of the rural tourism consumer
The rural tourism and, in particular, the agritourism have become increasingly popular activities, as a result of the
multiple benefits generated. The rural tourism is attractive for visitors with different motivations and different market
profiles. This paper aims to find the most common profile of the tourists visiting the rural areas. In order to segment the
rural tourism market, a series of customer indicators can be used, such as: reasons, preferences, needs and expected
benefits; geographical origin; economic and demographic status; psychographic characteristics and consumer behavior
etc. As the consumer needs and expectations regarding the rural tourism products are highly varied, there are several
types of tourists, the differences being determined mainly by geographical origin, but also by the different perceptions
on the quality of tourism products and services. In general, it can be said that these tourists come mainly from urban
areas, are middle-aged or above middle-aged, have a high level of education and training, have above-average incomes
and travel in small groups, usually with family and friends
Studii privind pretabilitatea soiului de viţă-de-vie Golia la cultivarea în sistem ecologic
In the North-eastern part of the country, the cultivation of vines in ecological system is a real challenge due to specific climatic conditions from the last years with an unfavorable impact on vineyards. In ecological viticulture the identification and cultivation of vine varieties with increased productivity and genetic resistance present a particular importance. In this regard, studies have been carried out on the behavior of Golia variety, a variety for white wines, in terms of agrobiological and technological value, under the condition of applying phytosanitary treatments with products based on copper and sulfur. The results obtained regarding the response to the attack of the main pathogens, as well as the productivity and quality characteristics, has been showed the possibility of gradual adoption and implementation of the ecological cultivation system of Golia variety, in the condition of the Copou-Iaşi viticultural ecosystem
Reducing SO2 content in wine by combining High Pressure and glutathione addition
The aim of this work was to examine the potential of High Pressure (HP) technology as an alternative technique to SO2 addition for red wine preservation. It focused on producing wines with reduced added SO2 and the simultaneous addition
of glutathione (GSH) as a natural antioxidant. Selected quality parameters of red wine samples from Mouchtaro grapes
treated by HP using various pressure parameters were tested. HP processing studied was applied at 200, 400 and
600 MPa for 0, 5 and 15 min. The application of HP for a long duration resulted in a significant reduction in phenolic
compound concentrations (TP) due to both extended polymerisation and reduced volatile acidity (VA) and acetic acid
concentrations (AAC), which in turn was mainly due to better antimicrobial protection. Based on the changes to the
contents of all TP, VA and AAC groups, processing for 5 min at 400 MPa was selected as the optimum HP condition.
Red wine samples from Mouchtaro grapes containing 0, 20, 40, 60, 80 and 100 mg/L of SO2 and 10 mg/L of GSH
were HP-treated under the selected pressure/time conditions. Untreated samples containing the same concentrations
of SO2 and GSH were used as control samples. Indices such as AAC, Antioxidant activity (AOA), total anthocyanins,
TP, mean Degree of Tannin Polymerisation (mDP) and the composition of volatiles was determined over a period of
12 months. Sensory analysis of the samples took place during the 12th month of storage. After the 12-month period,
the pressurised samples with GSH showed higher content of total aldehyde/ketone and higher-alcohols, consistently
lower concentrations of acetic acid, ethyl acetate and total esters, and lower VA values. Finally, based on the results
obtained from the sensory analysis, untreated samples were characterised by «Red fruits» odours, whereas treated
samples were distinguished by their «chocolate» aroma. These results suggest that HP could be used for the production
of more «mature» wines. A reduced SO2 concentration of up to 40 or 60 mg/L may be sufficient for wine stabilisation
when combining HP treatment and GSH additions, depending on grape variety
Sustainability in the Case of Small Vegetable Farmers: A Matrix Approach
Considering that sustainability is a relative concept, but also that this limitation could be avoided through continuous adaptation of the evaluation tools by taking into account the directions of change (such as time, space, application domain), the goal of the present study is to elaborate a matrix for measuring the level of sustainability for small vegetable farms. Thus, looking at what sustainability could represent under such circumstances, we divided its characteristics into four main dimensions (economic, social, environmental, cultural), while adding the private dimension. Inclusion of the private dimension may provide potential added value to this study, and thus enrich the general perspective of producers’ capacity to meet the sustainability goals in their entrepreneurial activity. To quantify these five dimensions of the sustainability matrix, a questionnaire was built up and used as support for face-to-face interviews conducted at the level of the North-East Development Region of Romania. Our results showed associations between diverse components of the dimensions considered, revealing their synergy in farm activity, along with the occurrence of some differences in the levels of sustainability dimensions and sub-dimensions, which differ as a function of the specific types of vegetable production (conventional, ecologic, natural, mixed)
Retrospective evaluation of notched and fragmented QRS complex in dogs with naturally occurring myxomatous mitral valve disease
Myxomatous mitral valve disease (MMVD) is the most common cardiac disease in dogs. The
association of QRS notching (nQRS) or fragmentation (fQRS) with disease severity is currently
unknown. The study objective was to assess the prevalence of nQRS and fQRS in dogs with
MMVD and its severity according to ACVIM classification and to compare the results with a
group of healthy dogs. This retrospective cross-sectional study included 34 healthy control
dogs and 155 dogs with spontaneous MMVD (42% of dogs in class B1, 23% in class B2 and
35% in class C). fQRS was defined as nQRS complexes in two contiguous leads in the frontal
plane (leads I and aVL) and (II, III or aVF). A one-way ANOVA with Bonferroni post-hoc test
was used to assess the differences in continuous data between control and MMVD groups.
Of the MMVD group, 58% showed nQRS in at least one lead and 27% presented fQRS. There
was no difference between the number of leads with a nQRS and disease severity (p ¼ 0.75)
nor did the number of leads with a nQRS correlate with left atrial size (r ¼ 0.48; p ¼ 0.5). The
number of dogs with fQRS did not differ among classes of MMVD (p ¼ 0.21). nQRS and fQRS
were more prevalent in dogs with MMVD compared to control dogs (p < 0.01). This study
did not identify any relationship between the number of leads with a nQRS and disease
severity. However, dogs with MMVD had a higher prevalence of nQRS and fQRS compared
to control group
Experimental Induction of Type 2 Diabetes Mellitus and the Efficiency of Bariatric Surgery in its Reversal in Rats
Following recent years, there is an increased body of literature on the connections that might exist between type 2 diabetes mellitus and the efficiency of bariatric surgery in its reversal compared to other medical approaches such as dieting. Aim. To induce experimentally type 2 diabetes mellitus in rats in order to observe the effects of bariatric surgery in the recovery as well as the reestablishment of normal insulin levels in order to extend the findings in house animals. Materials and methods. This study was conducted in three stages: the first consisted in inducing type 2 diabetes mellitus (T2DM) in 40 young Wistar male rats, by initially feeding them human food high in vegetal fats, oleaginous seeds, simple and complex carbohydrates, sugars, lipids, fats, proteins and fructose for a period of 8 weeks followed by a single low dose of streptozotocin (STZ), administered through intraperitoneal injection. The second stage of the study started when the rats became obese and therefore qualified for the bariatric procedure and the third stage consisted of post-operation supervision and care. The surgical procedure, performed on 10 obese rats, consisted in reducing the size of the stomach by partial gastrectomy of a 1.5 – 2.0 cm wide and 6.5 – 7.5 cm long area on the large curvature. Results. Showed rapid improvements in body weight and blood sugar control after 9 days. Conclusion. After putting the rats on a diet high in carbohydrates, sugars, lipids and fats and administering them STZ, the induction of type 2 diabetes was successful and the partial gastrectomy led to a better blood sugar control. The bariatric procedure provides a faster therapeutic response than conventional diets