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Researches regarding antimicrobial resistance and the associated zoonotic risk to Escherichia coli strains isolated from swine
The incidence of Mop-Top and Aucuba potato viruses in several Romanian areas (preliminary studies)
PMTV (Potato Mop-Top Virus ) an PAMV (Potato Aucuba Mosaic Virus) are new Romanian damaging pathogens of
potato crop. They can cause serious reduced yield and decrease of the tuber’s quality. In case of these viruses, infected
plants do not allways show symptoms and symptom depends on potato cultivar, virus strain and the environment.
PMTV and PAMV infection of material plants can produce spraing in tubers of sensitive varieties. PMTV cause heavy
losses in northen Europe and it is very difficult to control. Foliar symptoms include bright yellow blotch (Aucuba), a
’V’-shaped yellow pattern, deformed leaves and internode shortening. PAMV is a helping virus for devlopment of
dangerous potyvirus like Potato Virus Y (PVY). Elimination of all these pathogens from potato supply is essential for
potato production and the choice of resistant varieties to the infection with these viruses could be one of the measures
recommended for farmers and producers. The results of this preliminary study show that PMTV and PAMV were not
found in the material collected from the following varieties: Armonia, Azaria, Christian, Cosiana and Millenium
(samples collected from the counties Braşov, Covasna, Harghita, Suceava in 2019 and 2020)
Cytological and histopathological diagnosis in mast cell tumors in companion carnivores
Mast cells tumours are considered a member of the round cell tumour group and are one of the most frequent cutaneous
and subcutaneous neoplasms in companion carnivores (up to 21%), and are more common in dogs than in cats.They can
be diagnosed both through cytopathological and histopathological diagnostic.
In 2020-2021 we diagnosed 11 mast cell tumours in dogs and 2 in cats, out of which 8 through cytological and 5
through histopathological examination. The formations were located in various parts of the body, both cutaneous and
subcutaneous. The histopathological examination revealed round, oval, polygonal or even spindle-like cells that had
basophilic, intracytoplasmic granules and a central, large, round nucleus. The presence of eosinophils was not observed
in all the tumours. The mitotic index and anaplasia degree varied greatly, depending on the benign or malignant
character of the tumour.
The cytological examination revealed round cells of a mesenchymal origin that presented basophilic granulations in
various degrees. The number of granules varied from case to case, but generally speaking, these were much more easily
observed in cytological slides than in the histopathological ones
Environmental performance in EU countries from the perspective of its relation to human and economic wellbeing
The actual development challenges impose new criteria of national performance evaluation,
the concept of wellbeing tending to be measured not just in terms of economic and social dimensions,
but also vs. the environment. Accordingly, considering the national environmental performance
among the EU countries in 2006–2019 period, we grouped them and concentrated on the clusters
registering the highest and lowest levels, analyzing how the components of the human and economic
dimensions influence it. Applying panel data models, our main results emphasized that, firstly, for
the countries with a better environmental performance, sufficient drinking water, safe sanitation,
education, gender equality, and good governance were significant; in the countries with the lowest
levels of environmental wellbeing, sufficient food, sufficient to drink, education, and income distribu tion were insignificant, while the remaining components were relevant. Secondly, in both groups of
countries, organic farming and public debt were significant; nevertheless, differences were observed
for genuine savings and employment, for which the peculiarities of economic activities seemed to be
materialized as different influences upon environmental wellbeing. Our study draws alarm signals
regarding the development patterns applied in the EU, seeming to have results that strengthen the
sustainable goals, but not sufficient for exceeding the traditional growth-oriented model
Influence of technological parameters on the sensory quality of smoked pork chop samples
The importance of meat in consumption is due to its nutritional qualities, being a source of macronutrients, high-quality
proteins and lipids, and highly bioavailable micronutrients such as iron, zinc, selenium, phosphorus, vitamin A and the
B complex of vitamins. In addition to its nutritional quality, meat is also known for its sensory properties. The sensory
quality of meat is determined by the technological processes the raw material undergoes, such as maturing, salting and
heat treatments. By differentiating the parameters of the production technology (ageing time, salting degree, heat
treatment time and temperature), the sensory differences between the experimental batches were investigated. The
ageing time had a significant impact on the textural characteristics, the batches matured for 7 days showed lower
hardness and higher juiciness. The heat treatment parameters (time and temperature) mainly influenced the aroma,
imparting a slightly more intense burnt flavour to the batches subjected to the highest temperatures (P1 and P2). The
rancid flavour had subunit mean scores for all six experimental batches produced, with no significant differences. For
the colour attributes evaluated, no significant differences were observed between the experimental batches studied, the
highest average were scores achieved by P5 (8.08 ± 0.310 points) for colour intensity and P2 (8.43 ± 0.383 points) for
colour uniformity
Phyllosphere microbial profile of ornamental plants grown in indoor and outdoor environments
The aerial surfaces of plants, or phyllosphere, represent unique and wide habitats for microbial communities, which
play a key role in plant growth and adaptation to adverse conditions. The phyllosphere microbiota is composed mainly
of bacteria and fungi, and less frequently are present viruses, cyanobacteria, and protozoans. Leaf-inhabiting fungi and
bacteria are important, but often overlooked component of biodiversity studies. To understand their diversity and
function in relation to plant species and climate, the phyllospheres of five phylogenetically diverse ornamental plant
species (Vinca minor, Aster dumosus, Chrysanthemum indicum, Stachys lanata, and Sedum spectabile) were analyzed
under indoor and outdoor conditions. For both environment, Stachys lanata showed the lowest absolute abundance and
diversity of fungi and bacteria, while Aster dumosus had the highest abundance and the diversity of fungi in comparacy
to the other four plant species. Fungal isolates were abundantly ascomycetes and predominated by commonly known
endophytic genera, such as Penicillium, Aspergillus, Alternaria, Rhizopus, and Fusarium. In the outdoor environment
was a smaller proportion of shared fungal genera among the five different plants, in comparacy to indoor controlled
greenhouse. For a better knowledge of the impact of plant species, UV radiation, variable temperature, nutritional
resources, and relative humidity on microbial biodiversity, fungal genera from inside and outside cultivated species
were identified
Antibiotic Susceptibility Profile of Pseudomonas aeruginosa Canine Isolates from a Multicentric Study in Romania
Treating infections caused by Pseudomonas aeruginosa is increasingly difficult due to high antibiotic resistance, materialized through the presence of multiple resistance strains, as well as due to rapid development of resistance throughout treatment. The present survey was conducted to investigate the antibiotic susceptibility profile of Pseudomonas aeruginosa pathogens in two University Veterinary hospitals from different geographical regions of Romania (i.e., Southwest Timișoara county and Northeast Iași county) involved in superficial canine infections. A total of 142 swab specimens were collected from dogs with superficial infections (superficial skin infections, otitis externa, and perianal abscess) to assess the presence of Pseudomonas aeruginosa, based on phenotypic and molecular characterization. According to their confirmed morphological and molecular features, 58 samples (40.84%; 58/142) were positive for Pseudomonas aeruginosa (according to their confirmed morphological and molecular features). Antibiotic susceptibility testing for 12 antibiotics was conducted using the Kirby–Bauer disc diffusion method. Drug resistance was observed in the case of all tested antibiotics. The susceptibility rate of P. aeruginosa strains that were tested in this study was in the following order: ceftazidime (53.44%; 31/58), followed by aztreonam (51.72%; 30/58), amikacin (44.82%; 26/58), azithromycin (41.37%; 24/58), gentamicin (37.93%; 22/58), cefepime (36.20%; 21/58), meropenem (25.86%; 13/58), piperacillin-tazobactam (25.86%; 13/58), imipenem (22.41%; 13/158), ciprofloxacin (17.24%; 10/58), tobramycin (8.62; 5/58), and polymyxin B (1.72; 1/58). The results highlight the importance of antibiotic susceptibility testing in Pseudomonas aeruginosa isolates from dogs with superficial infections to use an adequate treatment plan to manage the skin condition and other pathologies (otitis externa and perianal abscesses)
Limiting factors of agricultural land in Cucuteni administrative territory, Iasi county
The soil is the result of the action of different processes determined by environmental factors, continuously adapting to
natural and / or artificial changes in the environment, recording and memorizing through certain phenomena, processes
and characteristics, the main moments of evolution. From the analysis of the geomorphological indicators, of the
Cucuteni territorial administrative unit, regarding the slope of the lands, landslides, deep erosion (ravenation) and
pedological indicators on organic matter content, soil reaction, soil texture, siltation / stagnation, salinization and
alkalization, result that the soils in the studied territory have a high degree of degradation. Among the limiting factors of
agricultural production, in descending order of affected areas, are: surface erosion (560.58 ha), landslides (506.53 ha),
pseudogleization (367.46 ha), acidification (318.05 ha), siltation (168.70 ha), salting (86.87 ha) and deep erosion (71.18
ha). Frequently, on the territory of Cucuteni commune, the limiting factors are associated on most of the lands, so that
their control requires a set of agro-improvement and special measures
Managementul integrat al controlului dăunătorului Tuta absoluta (molia minieră) la culturile de tomate din spații protejate
Tuta absoluta (tomato leafminer) (Meyrick) (Lepidoptera: Gelechiidae) is considered one of the main pests of tomato crops globally and since 2009, it represents a potential danger for tomato crops under high plastic tunnels in Romania. The attack is manifested through mines and galleries produced by young larvae in leaves, stems and fruits, in which they feed and grow. The experience done in 2021, at the R.D.I.V.F.G. Vidra, had as purpose the application of an integrated set of measures and means to control this pest (use of healthy seedlings for planting, use of insect-proof nets, dynamic monitoring of adult’s density using Delta traps and application of warning treatments for this pest control at two tomato hybrids. From the tested products those based on spinosad 240 g/l - 0.05%, chlorantraniliprol 120 g/l - 0.0175% and cyantraniliprol 100 g/l + acibenzolar-S-methyl 12.5 g/l - 0.125% had an efficiency between 68.64 and 79.96% (Prekos F1 hybrid), respectively 83.60 and 87.39% (Vitara F1 hybrid)