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The study of the main quality characteristics of vegetable seeds during the storage period to optimize preservation conditions
No full textEfficiency of microsatellite markers in genotyping of Orobanche cumana populations
No full textMicrosatellite (SSR) markers have been accepted and employed as useful tools for measuring genetic diversity and
divergence within and among populations. In this study, the utility of 15 SSR markers in discrimination of 33
Orobanche cumana (broomrape) populations from different geographical locations (Moldova, Romania, Bulgaria,
Serbia, Turkey, China) was assessed. A total of 279 O. cumana plants were genotyped and 110 alleles identified. The
level of genetic polymorphism of SSR markers was evaluated by calculating the effective number of alleles per locus
(Ne), which demonstrated an average of 5.243, Polymorphic Information Content index (PIC: 0.745), Nei's genetic
diversity (H: 0.782) and, Resolving power (Rp: 5.794). Most markers showed PIC values higher than 0.5, indicating a
big genetic polymorphism in O. cumana populations. Based on the Rp index and PIC values, genetic diversity in the
broomrape populations from Turkey (Rp: 4.774, PIC: 0.722) and Moldova (Rp: 4.394, PIC: 0.716) was higer than in
other populations. However, the studied microsatellite markers system characterized very well the genetic structure of
all O. cumana populations included in this study. Seven SSR markers (Ocum-052, Ocum-059, Ocum-074, Ocum-081,
Ocum-087, Ocum-196, Ocum-197) were selected based on the statistical analysis as the most informative and efficient
markers for measuring genetic diversity in O. cuman
Toxoplasmosis-a disease with high epidemiological risk in humans and animals
No full textInfections produced by the protozoan Toxoplasma gondii are widespread in humans and animals. Due to its lack of host
specificity, this parasite is able to infect a large number of hosts as well as different cell types. Although toxoplasmosis
is the most reported parasitic zoonosis in Europe, the incidence of the disease in humans and the presence of the parasite
in animals, food and water is underestimated. If acquired as an acute infection during pregnancy, Toxoplasma gondii
infection can have serious adverse effects on mothers, foetuses and newborns. Latent toxoplasmosis also causes a variety
of pathologies and has been linked to serious adverse effects on pregnancy. The study was conducted over a 2-year period,
2019-2020, in the Parasitology Clinic of the Faculty of Veterinary Medicine, Iasi, following the prevalence of reported
cases of toxoplasmosis in cats. Thus, out of 33 tests worked, no case of toxoplasmosis was recorded in cats, all serological
tests being done upon request. During 2020, 226 AB. ANTI TOXOPLASMA GONDII- IgM (ELISA) tests were performed
in the Praxis laboratory, of which only 15 were positive. All positive tests were identified only in women, of which 10 in
the age category 25-34 years, 4 in the age category 35-44 years and 1 case in the age category 15-19 years. In the Praxis
laboratory during 2020, 220 more AB. ANTI TOXOPLASMA GONDII- IgG (ELISA) tests were performed, out of which
72 positive cases were identified, 5 being positive in males in the age categories 0-12 months, 1 year and 15-19 years,
and the remaining 67 were identified in women in the following age categories: 0-12 months, 15-19 years, 20-24 years,
25-34 years, 35-44 years, 45-54 years and 55-64 years. The lack of positive cases in animals during the 2-year study, but
the high number of positive cases in humans during a single year, shows the major public health importance of the study,
as this very serious disease in pregnant women and immunosuppressed people is under-diagnosed in veterinary medicine
Evaluation of diet supplementation with wheat grass juice on growth performance, body composition and blood biochemical profile of carp (Cyprinus carpio L.)
Get PDFWheat grass juice (WGJ) is an extract of young wheat plantlets (Triticum aetivum L.) used
worldwide for its health related properties. In this study, the following feeds containing WGJ were
tested on common carp (Cyprinus carpio L.): Control (C), WGJ1% (V1), WGJ2% (V2) and WGJ4%
(V3) w/w. Fish with an average initial weight of 102 g/individual were grown in a recirculating
aquaculture system. The results showed that WGJ had stimulatory effects on growth performance.
Accordingly, final body weight increased by 11% at V1, 39% at V2 and 23% at V3, while other
indices (feed conversion ratio, specific growth rate, relative growth rate, protein efficiency ratio,
and condition factor) were unaffected. Body composition analyses revealed a significant decrease
in fat content at V2 and a significant increase in collagen and ash at the same variant, while the
protein content was unmodified. Regarding the blood profile, significant increases in the content of
albumin, globulin, total protein, and calcium were recorded in the variants with WGJ. The positive
results of WGJ on carp can be attributed to its biochemical composition, which is rich in chlorophyll
(4.71 mg mL1), total phenols (164 g mL1 gallic acid equivalents), and high antioxidant activity
(67% inhibition of DPPH 2,2-diphenyl-1-picrylhydrazyl). The results suggest WGJ can be used as a
promising feed additive for common carp
Greşeli frecvente la reprezentarea în triplă proiecţie ortogonală a cilindro-conicelor şi metode pentru eliminarea acestora
Get PDFIn many elements of street furniture (pergolas, terraces, benches, etc.) the straight cylinder (elliptical or circular) is found as a constructive element. In the execution drawings (plans, main or side views) of these elements, the correct representations in triple orthogonal projection in the clearance of the bodies are necessary. The paper shows the frequent mistakes of the representation in triple orthogonal projection in the shape of the straight cylinder (cylindrical or elliptical that has the base located in various planes (general position, vertical, parallel, or intersecting the OX axis) and propose a solution for avoiding misrepresentations
Research of the involvement of the members of the project teams in accessing the financing of the projects of the higher education institutions from Romania
No full textThis paper refers to the research of the acces needs of funds for university projects and involvement of project team
members. The research aimed at: Evaluating and analyzing from a quantitative point of view the links that characterize
the relationship between the needs of accessing project finance and the involvement of project team members, who are
also employees of the university. The study consisted in applying an investigation method based on the questionnaire
instrument that was designed in the original way regarding aspects such as: Identification of human resources – general
characteristics of the project team members as respondents; Types of projects accessed in higher education institutions;
Motivation and ways of accessing projects by universities; Expected results. The results of the researchers are
concretized in establishing the premises of the role of human resources of the university projects in accessing the funds
for their financing. The essence of the analyzes performed consists in conceptualizing the problem of obtaining the
financial resources necessary to carry out the projects and awareness of training and development of the human resource
involved
Research on the presence of viral infections in dogs in dog shelters from Eastern Romania (Botoșani, Iași and Vaslui Counties)
No full textThe present study aimed to investigate the presence of viral infections in dogs in paddocks in eastern Romania (Botoșani,
Iasi and Vaslui counties). The research was focused on the analysis of diagnostic methods used in paddocks to identify
infectious agents, the analysis of predisposing factors, as well as the analysis of measures to monitor and combat infections
in the shelter. Most cases of viral diseases (5,000) were observed in the case of a shelter in Iasi County. Canine parvovirus
was the most common viral disease among dogs in the three counties included in the study, the incidence of this disease
ranging between 28.18% and 100%. Cases of Carre's disease, infectious hepatitis and kennel cough were also diagnosed
in the paddocks included in the study, but their incidence was significantly lower. Among the main recommendations for
preventing canine parvovirus infection are avoiding contact between sick and healthy animals, as well as avoiding
frequenting of contaminated areas by their young pathways. In the case of the dog shelter located in Botoșani, the
impossibility of ensuring a proper quarantine before introducing the puppies into the shelter, led to a mortality of 87.91%
among the youth. The present results suggest that there were outbreaks of canine parvovirus in the shelters included in
this study, with CPV being the primary pathogen in all three shelters. Due to the significant losses caused by viruses and
the high costs of treating sick animals, there is a need for early diagnosis and the application of well-defined
immunoprophylaxis measures in shelters. This study reinforces the importance of immunization to ensure the welfare of
dogs in public and private shelters
Functional quality of improved tomato genotypes grown in open field and in plastic tunnel under organic farming
No full textIn response to urgent demand to raise awareness of the nutritional and health benefits of
tomato consumption and to advocate for healthy diets through increased sustainable production
and consumption of fruits and vegetables, this study is intended to promote a healthy and balanced
lifestyle, sharing the best practices of production and consumption. The aim of this research was to
compare the effects of the growing system (field vs. plastic tunnel) and of genotype characteristics for
organic improved tomato genotypes. The research was carried out in the 2019 and 2020 years on eight
improved tomato genotypes. The results showed that the ascorbic acid content presented higher
values for organic tomatoes cultivated in the field for all genotypes studied, with an upper limit of
18.57 mg 100 g1 FW. In contrast, the content in -carotene and lycopene showed higher values for
genotypes grown under plastic tunnel conditions. Significant statistical differences were noticed
concerning the mean values of all genotypes according to cultivation conditions (field vs. plastic
tunnel) for most parameters excepting total soluble solids (TSS), titratable acidity (TA), maturity index
(MI) and flavor index (FI). This highlights the major importance of the selection of some genotypes
of tomatoes that respond positively to the organic cultivation system in terms of the presence
of the antioxidants compounds (vitamin C, lycopene, and carotene) in representative quantities.
Genotype 3 is highlighted by the highest content in carotene (7.4 mg 100 g1 F.W.) and lycopene
(8.4 mg 100 g1 F.W.) and genotype 5 by the highest content in vitamin C (16.8 mg 100 g1 F.W.). The
results of the study suggest that by applying appropriate techniques for growing organic tomatoes
in the plastic tunnel system, the antioxidant substrate can be optimized compared to the results
obtained for the field system
Irrigation regime for bean beans in the conditions of the Romanian Plain
No full textUnlike other crops, beans have moderate moisture requirements, but are very sensitive to moisture deficiency during
flowering and grain formation. It has been established that in the short interval between these phenophases the
sensitivity is maximum both in case of excess moisture and moisture deficit. Research has shown that the lack of water
during flowering and fruiting greatly reduces production by shortening the duration of these phases of vegetation, by
reducing pollen fertility, reducing pods and grain weight. The sensitivity of beans to the deficiency of vapor saturation
in the atmosphere during flowering also has negative effects on the harvest. In order to determine to what extent the
years of experimentation approach or deviate from the climatic conditions typical of the experimental field, monthly
aridity indices were used for the series of years in which the experiments were performed at the studied farms,
compared to indices of aridity from a longer period. The highest yields were obtained when the soil moisture at the
beginning of flowering was at the field capacity for water and was maintained at the mentioned ceilings until the full
formation of the grains. This paper summarizes the results of research on the influence of irrigation on production
obtained in 2018-2021, at four farms located in the North Baragan Plain in Braila County