BIOREpository (Faculty of Biology, University of Belgrade)
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    Tiny Colonizers of Frog Skin: Composition and Diversity of Diatoms in the Pelophylax esculentus complex

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    Diatoms, microscopic algae found in various aquatic environments, have recently been recognised as epibionts on the skin of amphibians, although their diversity and ecological role remain underexplored. In this study, the diatom communities on the skin of frogs of the Pelophylax esculentus complex are investigated at three freshwater sites in southern Banat (Serbia), which differ in the degree of eutrophication and anthropogenic pressure. A total of 59 frogs representing all three taxa of the complex were collected and sampled using standard algological methods: adhesive tape and brush scraping. A total of 203 diatom taxa from 54 genera were identified. Statistical analyses showed that the environmental conditions significantly influenced the structure of the diatom community. A two-way ANOSIM revealed significant differences between localities (R = 0.23, p = 0.03), but not between frog taxa (R = 0.08, p = 0.24). PERMANOVA confirmed a significant effect of locality (F = 1.39, p = 0.03), with no significant effect of frog taxa (F = 12.35, p = 0.11) or their interaction (F = 10.64, p = 0.32). Principal coordinate analysis revealed two environmental gradients: The first separated sites according to trophic state (mesotrophic, eutrophic, hypertrophic), with diatom diversity higher in eutrophic conditions; the second reflected saprobial levels and distinguished oligosaprobic/β- mesosaprobic from α-mesosaprobic/polysaprobic habitats. The weak differences in the diatom communities between the frog taxa indicate that the environmental conditions and not the host taxa characterise these communities. This emphasises the ecological role of amphibian skin as a suitable microhabitat for periphytic algae and highlights the need for further research to determine whether it serves as a transient or stable habitat for diatom colonisation.M3

    Food choice and oviposition site preference in Drosophila melanogaster

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    Knjiga apstrakata, p. 268M3

    New Findings of the Little Known Crenobiotic Snail Bythinella serborientalis Radoman, 1978 (Gastropoda: Truncatelloidea, Bythinellidae) with Notes on its Morphological Variability

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    We report on two new sites for the little known crenobiotic snail Bythinella serborientalis Radoman, 1978, based on molecular and morphological analyses. A recent record of this species from western Serbia is discussed and corrected. In addition, we provide information about the intraspecific variability of B. serborientalis, both in terms of shell and penial morphology. In general, the morphological variability is observed as quite high and there are no stable characters which could distinguish this species from its congeners. Therefore, DNA analyses should be used in order to complement morphological descriptions.M230.517317877

    A Holistic Approach Model to Food Safety Surveillance: Detecting Toxoplasma gondii in Meat

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    Prevalences of infections caused by food- and waterborne parasites, some of them causing a significant disease burden, are on the increase. In the absence of systematic surveillance programmes for many of these parasites, particularly in extensive or free-range husbandry settings, research efforts are under way to design monitoring procedures and improve food safety, beyond the current standard of practice or of food safety legislation. An example is given of the commencement of these efforts, by presenting the experimental work package in a research project which entails testing of meat (heart tissue) of backyard poultry and game birds for the presence of Toxoplasma gondii gDNA. The high molecular prevalence of infection detected in some bird species, particularly in backyard chickens raised in rural areas, points to the need for comprehensive and timely surveillance programmes to be applied to all extensively farmed, or game animals and birds used for human consumption. Surveillance should include testing of animal feed as well as the environment (water and soil). However, until risk-based surveillance programmes are operational, meat safety will principally depend on adherence to prevention practices, such as pre-market product processing, as well as on educating consumers in hygienic handling and proper meat preparation practices.M24768066

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    BIOREpository (Faculty of Biology, University of Belgrade)
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