BIOREpository (Faculty of Biology, University of Belgrade)
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Irrigation systems as reservoirs of diverse and pathogenic Pseudomonas syringae strains endangering crop health
No full textPseudomonas syringae (Psy) is a widely distributed bacterial species complex primarily recognized as a foliar pathogen but also inhabits diverse environments, including water habitats, where strains closely related to agricultural pathogens have been identified. The connection between Psy-caused epidemics and its potential presence in nearby irrigation systems remains underexplored. This study comprehensively examined the Psy complex in the Danube-Tisa-Danube Hydrosystem (DTD) in Serbia, assessing its abundance, phylogenetic diversity, and pathogenic potential. To reduce the reliance on the time-consuming steps of isolation and identification, we developed novel high-specific primers and probes for precise detection of strains belonging to phylogroup 2 within Psy complex. Our results demonstrated that dPCR, coupled with highly specific and sensitive primers, outperformed both traditional plating and qPCR in detecting the Psy complex and phylogroup 2 in irrigation waters, making Psy diagnostics more effective. Phylogenetic analysis indicated high strain diversity within the DTD, identifying phylogroups 1, 2, 7, 12, and 13 and haplotypes linked to strains previously encountered in epidemics on sugar beet in Serbia. Notably, 66.67% of the isolates from the DTD were capable of inducing disease. Phylogroup 2 isolates displayed a broad host range, suggesting that the dissemination of Psy from DTD through irrigation, poses a substantial threat to crop health and agricultural productivity.M21a8.21003802
Performance Evaluation of Galvanometric Mirrors for Fast Scanning in Multimodal Laser Microscopy
No full textStrana 95M3
IS OXIDATIVE STRESS A PHYSIOLOGICAL COST OF PARENTAL CARE IN SCOLOPENDRA CINGULATA LATREILLE, 1829
No full textStr. 123M3
Wood Pigeons (Columba palumbus) and Mallards (Anas platyrhynchos) as Indicators for Toxoplasma Gondii Contamination of Different Ecosystems
No full textToxoplasma gondii is an intracellular, zoonotic food- and water-borne parasite of
cosmopolitan distribution. The parasite is able to infect all warm-blooded animals and
infection occurs as a consequence of ingestion. Based on published reports, the prevalence
of T. gondii infection in European wildlife species varies geographically, as does the impact
of infection on animal health, ranging from benign to lethal. T. gondii can be transmitted
to animals and humans via multiple routes, including from the environment. Food borne
transmission via meat may be dominant in some species of wildlife, yet permanent
exposure to the environmental reservoirs also facilitates infection via soil and water. A
number of publications from different European countries reported the presence of T.
gondii oocysts in both soil and water, thus raising the question on the contribution of these
reservoirs as sources of infection. The mechanisms of environmental transmission are the
least well understood, yet it is clear that ecosystem characteristics (terrain), as well as the
complex interactions between parasite reservoirs (soil and water) with climate conditions
(precipitation) are contributing factors. To investigate the role of soil and water in T. gondii
transmission, the presence of infection was analyzed in two common bird species, wood
pigeons (Columba palumbus, n=32) and mallards (Anas platyrhynchos, n=35). These
species have been selected as bioindicators due to different habitat preferences, and
particularly, feeding habits: direct contact with soil (wood pigeons) or water (mallards). The
presence of the parasite was evaluated by detection of T. gondii gDNA in heart tissue
(predilection site) using real-time qPCR. The results showed that 12.5% of wood pigeons
versus 22.9% of mallards were infected, suggesting the possibility of a greater exposure to
the parasite in aquatic and/or marshy ecosystems versus woodlands. The results further
indicate that water may have more significance in T. gondii environmental transmission
than previously anticipated. The findings from this study provide important insights into
environmental sources of T. gondii infection for wildlife.M3
Golden Jackals’ Winter Feeding Habits A One Health Perspective
No full textThe golden jackal (Canis aureus) is an autochthonous canid species in Serbia, with its population numbers among the highest in Europe. Following decades of continuous range expansion, the golden jackal now occupies nearly the entire country, adapting well to various habitats, including human-alerted ones. Previous studies have highlighted the species' opportunistic feeding behaviour, allowing it to adjust its diet to the seasonal availability of resources. This study examines the composition of golden jackals winter diet in Serbia's to understand their ecological role and interactions with human activities during the resource-scarce winter months. In collaboration with hunting associations, we analysed stomach contents from 86 legally hunted jackals collected during the winter months (December–February) of 2023 and 2024. Sixteen individuals had empty stomachs. Domestic ungulate carcasses were the dominant food category, occurring in 44 stomachs (62.9 % FO) and contributing 68.1% of total biomass, underscoring a heavy reliance on carrion. Small mammals were the most common live prey category (24.1 % O, 10.6 % B). Other dietary components included poultry, plant material, game species, and carnivores, though these were of lesser importance. The jackals' dependence on improperly disposed carcasses raises environmental and public health concerns, including the potential transmission of pathogens to domestic animals and humans. This highlights the need for a One Health approach to address these risks through improved carcass disposal and waste management practices, thereby mitigating human impacts on wildlife feeding behaviour and reducing public health risks.M3
Some Like It Rock ‘N’ Cold: Speleomycology of Ravništarka Cave (Serbia)
No full textCaves and other subterranean ecosystems are characterized by stable, low temperatures, high humidity, and limited nutrient input, creating unique environments for extremophilic microorganisms. Among them, fungi play key roles in organic matter degradation, mineral interactions, and biogeochemical cycling, yet the diversity and adaptations of cold-adapted fungi in cave habitats remain insufficiently explored. This study investigated psychrophilic and psychrotolerant fungi inhabiting the stone surfaces of Ravništarka Cave in Eastern Serbia. Biofilm samples were collected from nine sites and analyzed using culture-based isolation on both nutrient-rich and diluted media, followed by incubation at 10 °C, 25 °C, and 37 °C. Fungal identification combined morphological characteristics with molecular analyses of the ITS region and BenA gene, while ecological roles were assigned using FUNGuild. A total of 41 fungal species were documented, spanning Ascomycota (53.1%), Basidiomycota (43.7%), and Mortierellomycota (3.1%) phyla. The genus Penicillium exhibited the greatest species richness, with 14 taxa documented, including P. chrysogenum, P. glandicola, and P. solitum, all previously associated with cold or oligotrophic environments. The psychrotolerant species Mortierella alpina was the only representative of Mortierellomycota. Ecological guild assignment revealed fungi functioning at different trophic levels, highlighting their multifunctional ecological roles in extreme subterranean habitats.M214.2706111
Efficacy and Potency of Novel Pyrimidine-Based Inhibitors of Ecto-5′ Nucleotidase/CD73 in Primary Astrocytes In Vitro
No full textM3
Erythron profile and liver enzymes as potential biomarkers of water and sediment pollution in vimba bream (Vimba vimba) from the Danube River: effects of varying pollution and sampling season
No full textBook of Abstracts 107M3
