BIOREpository (Faculty of Biology, University of Belgrade)
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Responses of Epilithic Diatoms to the Construction of Small Hydropower Plant in a Serbian River
No full textEnergy of running water accounts for almost 3/4 of the global supply of renewable energy sources and almost 1/5 of total electricity generation. Large and small hydropower plants are associated with a number of environmental problems. The main aim of this study is to investigate the impact of SHP on the diatom community. A total of 149 diatom taxa classified in 47 genera were identified, some of which are considered invasive (Achnanthidium druartii), rare (Gomphonema zellense, Frustulia amphipleuroides) and Navicula radiosafallax is first finding for Serbia. The impact of SHP is evident considering diatoms diversity and relative abundance of dominant species in three seasons (summer, autumn and winter). Values of most measured physico-chemical parameters changed immediately downstream of the SHP. Based on most diatom indices, the water of the Ljuboviđa River had a good ecological status. TID and TDI indicated a predominantly moderate water quality of the Ljuboviđa River. According to Serbian regulations, the Ljuboviđa River had a high ecological status.M221.324
ANTI-FERROPTOTIC ACTION OF SULFORAPHANE IN SKELETAL MUSCLE OF DIABETIC MICE
No full textSarajevo, Bosnia and Hercegovina, June 3-6, 2024, PC08M346
ISOLATION AND CULTIVATION OF CHROOCOCCUS (CYANOBACTERIA) FROM AEROPHYTIC BIOFILM IN STOPIĆ CAVE
No full textM3
DUAL INHIBITION OF ECTO-5'-NUCLEOTIDASE (CD73) AND ADENOSINE A2A RECEPTOR REDUCES NEUROINFLAMMATION AND OXIDATIVE STRESS IN TNF, IL1-Α, C1Q-INDUCED NEUROTOXIC ASTROCYTES
No full textM3
Prolonged intermittent theta burst stimulation restores the balance between A2AR- and A1R-mediated adenosine signaling in the 6-hydroxidopamine model of Parkinson’s disease
No full textAn imbalance in adenosine-mediated signaling, particularly the increased A2AR-mediated signaling, plays a role in the pathogenesis of Parkinson’s disease. Existing therapeutic approaches fail to alter disease progression, demonstrating the need for novel approaches in PD. Repetitive transcranial magnetic stimulation is a non-invasive approach that has been shown to improve motor and non-motor symptoms of Parkinson’s disease. However, the underlying mechanisms of the beneficial effects of repetitive transcranial magnetic stimulation remain unknown. The purpose of this study is to investigate the extent to which the beneficial effects of prolonged intermittent theta burst stimulation in the 6-hydroxydopamine model of experimental parkinsonism are based on modulation of adenosine-mediated signaling. Animals with unilateral 6-hydroxydopamine lesions underwent intermittent theta burst stimulation for 3 weeks and were tested for motor skills using the Rotarod test. Immunoblot, quantitative reverse transcription polymerase chain reaction, immunohistochemistry, and biochemical analysis of components of adenosine-mediated signaling were performed on the synaptosomal fraction of the lesioned caudate putamen. Prolonged intermittent theta burst stimulation improved motor symptoms in 6-hydroxydopamine-lesioned animals.
A 6-hydroxydopamine lesion resulted in progressive loss of dopaminergic neurons in the caudate putamen. Treatment with intermittent theta burst stimulation began seven days after the lesion, coinciding with the onset of motor symptoms. After treatment with prolonged intermittent theta burst stimulation, complete motor recovery was observed. This improvement was accompanied by downregulation of the eN/CD73-A2AR pathway and a return to physiological levels of A1R-adenosine deaminase 1 after 3 weeks of intermittent theta burst stimulation. Our results demonstrated that 6-hydroxydopamine-induced degeneration reduced the expression of A1R and elevated the expression of A2AR. Intermittent theta burst stimulation reversed these effects by restoring the abundances of A1R and A2AR to control levels. The shift in ARs expression likely restored the balance between dopamine-adenosine signaling, ultimately leading to the recovery of motor control.M215.
Impaired olfactory performance and anxiety-like behavior in a rat model of multiple sclerosis are associated with enhanced adenosine signaling in the olfactory bulb via A1R, A2BR, and A3R
No full textThe present study shows that animals with experimental autoimmune encephalomyelitis (EAE) exhibit olfactory dysfunction and impaired general cognitive abilities, as well as anxiety-like behavior. Olfactory dysfunction occurs on average at 2 dpi, well before the onset of the first motor signs of EAE (8-10 dpi). After the initial olfactory dysfunction, the EAE animals show a fluctuation in olfactory performance that resembles the relapsing-remitting course of human MS. The study also shows severe neuroinflammation in the olfactory bulb (OB), with numerous infiltrated CD4+ T cells and peripheral macrophages in the superficial OB layers, marked microgliosis, and massive induction of TNF-α, IL-1β, and IL-6. Reduced tyrosine hydroxylase activity in the glomerular layer, pronounced granule cell atrophy, and reduced numbers of type B neuroblasts in the rostral migratory stream also indicate altered plasticity of the neuronal network in the OB. Considering the exceptionally high purinome expression in the OB, the possible involvement of purinergic signaling was also investigated. The study shows that macrophages infiltrating the OB overexpress A3R, while highly reactive microglia overexpress the adenosine-producing enzyme eN/CD73 as well as A2BR, A3R, and P2X4R. Given the simultaneous induction of complement component C3, the results suggest that the microglial cells develop a functional phenotype of phagocytizing microglia. The study also demonstrates transcriptional and translational upregulation of A1R in mitral and tufted cells, which likely influence resting network activity in OB and likely contribute to olfactory dysfunction in EAE. Overall, our study shows that olfactory dysfunction and altered social and cognitive behavior in EAE are associated with increased adenosine signaling via A1R, A2BR, and A3R.M214.214079751
Properties of Extracellular Protease—Regulator of Hemostasis Produced by Micromycete Aspergillus tabacinus
No full textThe extracellular protease with protein C-like and plasmin-like activities was isolated from the culture fluid of the micromycete A. tabacinus BEOFB3260m. It has been established that A. tabacinus extracellular protease is a non-glycosylated serine protease with molecular weight about 30 kDa. The enzyme is active and stable at 25–37°C, active at pH 7.0–12.0 and stable at pH 3.0–12.0 and is a promising candidate for the development of new anticoagulant drugs.M231.011812360
Walnut supplementation increases levels of UCP1 and CD36 in brown adipose tissue independently of diet type
No full textDietary interventions that modulate the brown adipose tissue (BAT) thermogenic activity could represent a promising therapy for metabolic disorders. In order to examine if dietary walnuts intake regulates the expression of BAT thermogenic markers levels in healthy and metabolically challenged (fructose fed) animals, rats were initially divided into the control and fructose-fed groups. After nine weeks, these groups were subdivided into the one kept on the original regimens and the other supplemented with walnuts. High-fructose diet resulted in an increased relative BAT mass and no change in UCP1 content, while the walnut supplementation increased the amount of UCP1 in BAT, but did not affect 5-HT, NA, DHPG content and DHPG/NA ratio regardless of the diet. Moreover, the CD36 levels were increased following the walnut consumption, unlike FATP1, GLUT1, GLUT4, and glycogen content which remained unchanged. Additionally, the BAT levels of activated IR and Akt were not affected by walnut consumption, while ERK signaling was decreased. Overall, we found that walnut consumption increased UCP1 and CD36 content in the BAT of both control and metabolically challenged rats, suggesting that FFAs represent the BAT preferred substrate under the previously described circumstances. This further implies that incorporating walnuts into the everyday diet may help to alleviate some symptoms of the metabolic disorder.M223,517351745479