BIOREpository (Faculty of Biology, University of Belgrade)
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MicroRNA 146a-5p as a predictor of response to immunosuppressive therapy in acquired autoimmune myasthenia gravis
No full textBook of abstracts: 41-42M3
Pleurotus ostreatus cultivation for more sustainable soybean and sunflower seed waste management
No full textThe seed and edible oil (SEO) industry produces a significant amount of waste, from wastewater to organic solid waste such as soybean and sunflower seed husks. The inclusion of these materials as substrates for edible mushroom production was examined. Lignocellulosic characterization of the substrates was also performed to determine the exact nutrient requirements for fungal inoculation. Comparative analysis with the control sample cultured on traditional wheat straw showed that the solid wastes of SEO not only allowed a satisfactory growth rate of Pleurotus ostreatus, but also resulted in fruiting bodies with satisfactory sensory characteristics, chemical composition, and functional properties such as antioxidant activity. For the first time, emphasis was placed on cytotoxic and genotoxic properties to obtain baseline data for risk assessment. The results show that the maximum shelf life of P. ostreatus that allows overall quality to be maintained is seven days, as there is a genotoxic risk if the mushrooms are stored longer. Although the biological efficiency was 23 % on the day 14 which leads to increased growth of psychrotrophic microorganisms (2.65–3.20 log CFU/g). Considering the resource-intensive SEO industry and the significant amounts of waste associated with it, incorporating waste streams from this industry and converting them into valuable food contributes toward the research-backed realistic new avenues and moves beyond the status quo of the SEO industry.M213.90011386633
Axenic cultivation of bryophytes: growth media composition
No full textBryophytes were among the first plants to colonise the terrestrial environment. They do not possess root systems but are adapted to uptake nutrients through their entire body surface. However, the nutritional requirements of bryophytes remain poorly understood since they have no economic importance and are usually considered to be the same as those of tracheophytes. It is generally assumed that they can develop on poor substrates and easily fulfil their nutritional needs in resource-scarce environments. In this study, we analysed the media contents used for the axenic in vitro growth of bryophytes to infer their nutrient requirements and specificities. All media types previously applied in any reported axenic in vitro culture of bryophyte species were taken into consideration and an overview of bryophyte nutritional demands is provided, including any peculiarities and the advantages of certain media types.M222,3160
Photoswitchable opto-Lab-on-a-Chip for real-time and label-free cell analysis via classical and quantum holographic imaging
No full textStrana 51M3
The edaphic factor and orchids: Gymnadenia conopsea from contrasting geologies in the Central Balkans
No full textTwo different strategies for the distribution of macro- and trace elements can be observed in the terrestrial orchid Gymnadenia conopsea. Most trace elements are not translocated to the above-ground parts, whereas for macro-elements the trend was reversed, with the highest accumulation in the distal parts of the plants. Edaphic stress is one of the main factors affecting plant fitness, but it is still poorly understood, even in rare plants such as orchids. Gymnadenia conopsea is a terrestrial orchid that grows on different geological substrates, making it a model species for the study of adaptive responses to edaphic factors, including metals in soil. The samples of plant tissues of G. conopsea growing on carbonate, ultramafic and siliceous substrates in Serbia and the associated rhizosphere soil were collected and analysed for elemental concentrations. Two different strategies for the distribution of macro- and trace elements were found, corresponding to the trend generally observed in orchids. Trace elements (As, B, Cr, Co, Fe, Mn, and Ni) remain mainly in the underground organs and only a small proportion is transferred to the shoots. It was the opposite for the macroelements (Ca, Mg, K and P) with the highest accumulation occurred in the leaves and inflorescences. The tolerance of G. conopsea to the different geological substrates results from the moderate metal concentrations in the soils analysed and the exclusion strategy of the species, which is the most common response to metal induced stress in orchids.M213.638261
Evaluation of Cinnamon Essential Oil and Its Emulsion on Biofilm-Associated Components of Acinetobacter baumannii Clinical Strains
No full textAcinetobacter baumannii, one of the most dangerous pathogens, is able to form biofilm structures and aggravate its treatment. For that reason, new antibiofilm agents are in need, and new sources of antibiofilm compounds are being sought from plants and their products. Cinnamon essential oil is associated with a wide spectrum of biological activities, but with a further improvement of its physicochemical properties it could provide even better bioavailability. The aim of this work was the evaluation of the antibiofilm properties of cinnamon essential oil and its emulsion.M214.30010614
ParAquaSeq, a Database of Ecologically Annotated rRNA Sequences Covering Zoosporic Parasites Infecting Aquatic Primary Producers in Natural and Industrial Systems
No full textAmplicon sequencing tools such as metabarcoding are commonly used for thorough characterisation of microbial diversity in natural samples. They mostly rely on the amplification of conserved universal markers, mainly ribosomal genes, allowing the taxonomic assignment of barcodes. However, linking taxonomic classification with functional traits is not straightforward and requires knowledge of each taxonomic group to confidently assign taxa to a given functional trait. Zoosporic parasites are highly diverse and yet understudied, with many undescribed species and host associations. However, they can have important impacts on host populations in natural ecosystems (e.g., controlling harmful algal blooms), as well as on industrial-scale algae production, e.g. aquaculture, causing their collapse or economic losses. Here, we present ParAquaSeq, a curated database of available molecular ribosomal sequences belonging to zoosporic parasites infecting aquatic vascular plants, macroalgae and photosynthetic microorganisms, i.e. microalgae and cyanobacteria. These sequences are aligned with ancillary data and other information currently available, including details on their hosts, occurrence, culture availability and associated bibliography. The database includes 1131 curated sequences from marine, freshwater and industrial or artificial environments, and belonging to 13 different taxonomic groups, including Chytridiomycota, Oomycota, Phytomyxea, and Syndiniophyceae. The curated database will allow a comprehensive analysis of zoosporic parasites in molecular datasets to answer questions related to their occurrence and distribution in natural communities. Especially through meta-analysis, the database serves as a valuable tool for developing effective mitigation and sustainable management strategies in the algae biomass industry, but it will also help to identify knowledge gaps for future research.M215.5e1409
Strain-Dependent Differences in Inflammatory/Immune Activity in Cutaneous Wound Tissue Repair in Rats: The Significance of Body Mass/Proneness to Obesity
No full textInflammatory/immune cells and mediators are substantial for wound healing because they orchestrate biological activities in this complex process. Among factors that affect wound healing, obesity, and metabolic diseases are among the most significant, particularly because of a relationship between obesity and a prediabetic state with immune reactivity. Using Dark Agouti (DA) and Albino Oxford (AO) rats, which differ in immune responses as well as in proneness to obesity, we examined the impact of these intrinsic factors on cutaneous wound healing. Dynamics of the process were monitored at days 3, 5, and 7 post-wounding parallel in both rat strains by analysis of selected basic aspects of the wound repair process (cytokine and growth factor responses) in granulation tissue. Strain-related differences in the extent of reduction of the wound area were shown, which coincided with differential proinflammatory and immune-regulatory cytokines, as well as growth factors response in these rats. Some of these differences seem related to their dissimilarities in the proneness to obesity. Results in this study extended so far known differences in inflammatory/immune responses to a variety of stimuli between AO and DA rats and showed, for the first time, immune-based differences in wound healing between rats that differ in body mass (BM) and obesity proneness (under ad libitum feeding conditions with normal rodent chow).M224.455255572025
