BIOREpository (Faculty of Biology, University of Belgrade)
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    Antihyperglycemic Activity of Alchemilla viridiflora Herb Methanol Extract in Streptozotocin-Induced Diabetic Male Rats

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    Based on the traditional use of Alchemilla L. species for the treatment of diabetes, the effect of the methanol extract of Alchemilla viridiflora (AVM) on enzyme activity in vitro and its impact on blood glucose levels in vivo were investigated. Diabetes was induced in male Sprague-Dawley rats using streptozotocin. AVM was administered to both normal and STZ-diabetic rats for 20 days at three different doses. Blood glucose levels and body weights of the treated animals were monitored throughout the experiment. After 20 days, serum insulin, cholesterol, triglycerides, and high- and low-density lipoproteins were measured. In addition, a histological analysis of the pancreas was performed. The AVM demonstrated inhibitory effects on the activities of all tested enzymes. In the in vivo experiment, a statistically significant reduction in body weight was observed in the AVM-treated animals at all three doses compared with the normal control group. Notably, a dose of 200 mg/kg significantly decreased blood glucose levels on both the 10th day and 20th day (p < 0.05). However, the extract showed no statistically significant effects on the tested biochemical parameters. Overall, the results of this study suggest that AVM has potential for the treatment of hyperglycemia associated with diabetes and obesity.M214,6002819301

    Detection of Echinococcus spp. in Condemned Livers

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    Echinococcosis, a parasitic zoonosis caused by Echinococcus spp. tapeworms, poses a significant public health concern in Europe, but remains under-reported in both humans and animals. Post-mortem examination of slaughtered livestock offers a valuable opportunity for early detection and surveillance of parasitic infections, including echinococcosis, in meat intended for human consumption. In this study, liver samples exhibiting pathological changes were collected from two abattoirs in Serbia. A total of 31 livers—22 from pigs, 7 from lambs, and 2 from bullocks—were analysed microscopically, and molecularly using PCR, to detect Echinococcus spp. DNA. The parasite was detected in three pig livers, and three distinct species were identified: E. granulosus, E. canadensis, and E. multilocularis. Although the sample size was relatively small, the detection of multiple Echinococcus species in pigs suggests a potential role of these animals in the parasite’s transmission cycle within Serbia. These findings underscore the ongoing importance of comprehensive meat inspection protocols in abattoirs for zoonotic disease surveillance. They also highlight the need for expanded surveillance efforts and enhanced diagnostic procedures, particularly speciation of the tapeworm, to improve early detection and control of Echinococcus infections at the abattoir level.M24818666

    Balancing Act: Enhancing Student Comprehension of Acid-Base Physiology Through Interactive, System-Based Teaching

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    Acid-base physiology is widely recognized as one of the most conceptually challenging topics in undergraduate education due to its interdisciplinary nature and the necessity for both mechanistic and integrative understanding. This manuscript presents a structured, system-based teaching framework designed to enhance student comprehension of acid-base balance by promoting active learning and conceptual integration across organ systems. The instructional model is organized around four core modules: mastering terminology, identifying acid sources, exploring regulatory mechanisms, and understanding acid-base disorders. Notably, the approach introduces a specific sequence for concept delivery, supported by interactive in-class activities such as multiple-choice questions, open-ended prompts, pattern recognition tasks, physiological reflex analysis, and the construction of concept maps. This format encourages students to link molecular mechanisms with systemic outcomes and fosters the application of knowledge to clinical and everyday life scenarios.M221.

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    BIOREpository (Faculty of Biology, University of Belgrade)
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