BIOREpository (Faculty of Biology, University of Belgrade)
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Diversity of fungi isolated from the carapace of the European pond turtle (Emys orbicularis, L. 1758) in South Banat, Serbia
No full textThis study aimed to isolate and identify a fungal community on the carapace of the European pond turtle (Emys orbicularis) and to discuss their potential role as constituents of the turtle mycobiome. The study was conducted during the summer of 2020 within the special nature reserve Deliblato Sands (Deliblatska Peščara), situated in northern Serbia. We used a direct microscopy technique along with culture-based methods and molecular barcoding of the internal transcribed spacer (ITS) region. A plethora of fungal spores were documented as constituents of the biofilm present on the carapace surfaces. A total of 12 fungal isolates from the E. orbicularis carapace were identified, belonging to Alternaria, Aureobasidium, Cladosporium, Curvularia, Didymella, Microsphaeropsis, Pseudopithomyces, Naganishia, Rhodotorula, and Mucor genera. Ecological analyses showed that the majority of documented fungal species originated from terrestrial and aquatic environments, and many could be regarded as potential chelonian pathogens. The study presents pioneering research on fungal dwellers of E. orbicularis in Serbia.M230.718519577
Contribution to the knowledge on the distribution of Aristolochia clematitis in riparian and roadside habitats of Serbia
No full textBook of Proceedings: pp. 108-114M3
SEASONAL DYNAMICS OF DIATOM DIVERSITY IN THE PONORAC CAVE STREAM (RAVNIŠTARKA CAVE, SERBIA)
No full textProceedings BookM3
Thymus praecox subsp. polytrichus: Antimicrobial and antidiabetic activity of ethanol extracts
No full textThymus praecox is one of the most widespread species in the Euro-Siberian region. This study was conducted to determine the in vitro antimicrobial and antidiabetic activity of its plant extracts prepared with 70% and 96% ethanol, with and without
pretreatment with n-hexane, using the Soxhlet apparatus. The MICs and MBCs ranged from
0.035 to 0.150 mg/mL and from 0.075 to 0.300 mg/mL, respectively. Both 96% ethanol extracts
showed the most promising antibacterial activity, especially against Bacillus cereus, Enterobacter cloacae and Salmonella Typhimurium. The pretreated 96% ethanol extract also showed
remarkable antimicrobial activity against Staphylococcus aureus. Bacteria B. cereus, S.
aureus and S. typhimurium were also sensitive to the pretreated 70% ethanol extract, while
70% ethanol was most effective against S. aureus and Escherichia coli. The MICs and MFCs
varied from 0.017 to 0.30 mg/mL and from 0.035 to 0.70 mg/mL, respectively, with Aspergillus
versicolor and Trichoderma viride being the most sensitive. The IC50 values ranged from 0.94
to 1.40 mg/mL for α-amylase and from 76.10 to 610.2 µg/mL for α-glucosidase. The 70%
ethanol extract, especially with the hexane pretreatment, showed the highest enzyme inhibitory activity in the α-glucosidase assay (76.10 µg/mL), even higher than the control, acarbose/
glucobay (20.46 µg/mL). The highest α-amylase inhibitory effect was obtained for the 96%
ethanol extract. The results show that the extracts of T. praecox subsp. polytrichus have
significant antimicrobial and antidiabetic potential and can be used for food preservation
and phytopharmacy.M52172614
Molecular detection of Echinococcus spp. in pigs
No full textEchinococcus tapeworms of the Taeniidae family can infect humans and animals, causing seriousand potentially fatal disease. The clinically significant tapeworm species in Europe areEchinococcus granulosus, the causative agent of cystic echinococcosis and Echinococcusmultilocularis, which causes alveolar echinococcosis, the most severe form of the disease. Humansbecome infected after accidental ingestion of tapeworm eggs, which can contaminate food andwater. Tapeworm eggs are produced only in the intestines of definitive hosts (Canidae andFelidae), while intermediate hosts (ungulates) act as reservoirs of infection for definitive hosts.Traditionally, the epidemiological cycle of the tapeworm between sheep-dogs-people has beenconsidered to be of primary significance for the maintenance and spread of infection in the humanpopulation, while the role of other reservoirs has been neglected. To ascertain the presence ofEchinococcus spp. in domestic pigs, liver samples with discernible pathological changes inmorphology (n=27) were obtained from abattoirs. Pieces of tissue or cyst content (liquid or solid)were removed for genomic DNA (deoxyribonucleic acid) extraction and subsequent multiplexPCR (polymerase chain reaction) for Echinococcus species identification. Echinococcus spp. DNAwas detected in three samples and three different species were identified: Echinococcusgranulosus, Echinococcus canadensis and Echinococcus multilocularis. Despite the fact that alimited number of samples was investigated and that the Echinococcus tapeworm was not adominant finding, the presence of three different species of tapeworm is nonetheless concerning.These findings suggest that more extensive research is necessary to obtain reliable data on theprevalence of infection and diversity of Echinococcus spp. in pigsM3
Alpha, Beta and Gamma Taxonomy of Biocontrol Agent Diaeretiella rapae (Hymenoptera, Braconidae)
No full textDiaeretiella rapae (McIntosh, 1855) is a cosmopolitan koinobiont endoparasitoid of aphids, occurring mainly on crucifers and cereals. From description, it has changed several genera and has about 20 synonyms. The specimens for this study were collected between 1989 and 2023 from sites across Europe and the Middle East. For molecular analysis, the barcode mitochondrial gene COI was used, and morphological analysis was conducted with other Aphidius species. Morphologically, D. rapae falls within the determined variability of the same characters of the genus Aphidius. Dieretiella rapae comprised 23 haplotypes with a mean genetic distance between haplotypes of 0.8%. Phylogenetically, D. rapae is nested within Aphidius species with a genetic distance of 2.1% to 11.3%, which is within the range of other Aphidius species. Our results on morphological and molecular level confirm that D. rapae belongs to the genus Aphidius.M21a2.973616
Biosynthesis and health promoting traits of green synthesized cobalt oxide nanoparticles
No full textNanomedical applications have increased significantly. This work aimed to fabricate and characterize cobalt oxide nanoparticles (CoOnps) synthesized biologically via aqueous Alhagi maurorum extract and evaluate their cytotoxic and antimicrobial impacts. Green-synthesized CoOnps were prepared and analyzed using UV-Vis spectrophotometer UV-vis, Scanning electron microscopy (SEM), Transmission electron microscopy TEM, Energy dispersive X-ray analysis EDAX, Fourier transform infrared, FTIR, and X-ray diffraction (XRD). In vitro traits of green-synthesized CoOnps were studied on ovarian cancer cells (SKOV3) using a Sulforhodamine B (SRB) method. The cytotoxic effect and IC50 were estimated. Moreover, concentrations of 10, 30, 40, 70, 100, 200, 300, 400 and 500 μg/mL CoOnps were applied to investigate their antimicrobial effect against Listeria, Staphylococcus aureus and Streptococcus as gram +ve pathogenic bacteria, Bifidobacterium bifidum 2203, Bifidobacterium bifidum LMG 10,645, Bifidobacterium breve LMC 017, Bifidobacterium angulatum 2238 and Bifidobacterium longum ATCC 15,707 as probiotics, E. coli as gram -ve bacterial model and yeast strain Candida albicans. CoOnps showed anti-ovarian cancer effects at 24.02 μg/mL. Furthermore, it exerted antimicrobial activity versus Listeria, Streptococcus, S. aureus, and E. coli were 31.66 ± 0.88, 24.33 ± 2.08, 25.66 ± 0.33, and 33.00 ± 6.08; however, they did not suppress the growth of Candida albicans and all tested Bifidobacterial strains up to concentrations of 500 μg/mL with significant difference compared to all concentrations p < 0.05. Green synthesis of CoOnps is a low-cost, eco-friendly and easily prepared method. Its impressive features as cytotoxic SKOV3, a cell line ovarian cancer and antibacterial effect for some gram +ve and -ve bacteria, besides maintaining probiotics, could candidate them as competitive agents for medical, pharmacological, agricultural and food applications.M213.972715
Sweeteners affect biofilm formation and virulence gene expression in Pseudomonas aeruginosa PAO1
No full textPseudomonas aeruginosa is an opportunistic pathogen able to form biofilms, contributing to its virulence. With the increasing use of sweeteners in various foods, understanding their influence on bacterial behavior is critical. This study investigated the virulence of P. aeruginosa PAO1 exposed to sweeteners (erythritol, stevia, fructose, coconut sugar, cane sugar, demerara). Sweeteners didn’t affect growth rates. Erythritol stimulated biofilm (100 µg/mL, 159.98% formation), while 10 µg/mL of coconut sugar, cane sugar, and demerara promoted lower levels (∼70% formation). Erythritol stimulated exopolysaccharides production but reduced biofilm eDNA. Stevia, fructose, and coconut sugar increased the expression of lasI, lasR, rhlI, rhlR, pqsA, mvfR, and pvdF. HPLC analysis confirmed sucrose as the major sugar in demerara, coconut and cane sugar. Erythritol stimulated biofilm and some virulence genes expression, while other sweeteners’ effects varied. Cane sugar was a biofilm inhibitor with a limited gene expression effect. The sweeteners’ impact on microorganisms is diverse and should be further investigated.M232.651252241
Nationwide SMA Screening in Serbia: Results and Impact After Sixteen Months of the First Genetic Newborn Test
No full textOral and Poster Abstracts of the 13th ISNS European Regional Meeting. International Journal of Neonatal Screening. 2025; 11(1):21. Page: 78-79, P82M3