REPONIVS - Repository of Scientific Institute for Veterinary Medicine of Serbia
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Coenurosis of sheep in Serbia - case report
No full textCoenurosisa is a zoonotic disease caused by the larval forms of Taenia multiceps which are great host's animals from the family of canids. The disease is global distribution but is most present in the Mediterranean, Middle East, Central Africa and Australia. Larvae exhibit tropism toward brain tissue, and cysts are usually located in the left hemisphere of the brain of small ruminants. Involvement of the brain can cause increased intracranial pressure, seizures, loss of consciousness, and focal neurologic deficits. During 2022 we examinee one flocks of 78 milking sheep reared on hilly pastures located below the Zmajevac hill (397 m), between the branches of the Osimćki mountains and the river Zapadna Morava, at an altitude of 178 m. Symptoms of ataxia, such as unsteady gait and stumbling, and depression were observed in 12 animals. In 5 sheep, continuous aimless or circular movement, wandering, was also manifested. In addition to these symptoms, in one sheep that died, visual disturbances were observed depression and unilateral blindness. All affected sheep were slaughtered and a pathoanatomical examination was performed. In the brain of all animals we revealed the presence of infestation with one to four coenuri 4.1-5.5 cm in size. The sites of predilection were the left hemisphere (48%), followed by the right hemisphere (40%) and the cerebellum (12%). The coenurus has a thin wall surrounding a single cavity that contains a clear fluid. When the cysts were opened, numerous scoleces were found inside, from 2 to 3 mm in diameter attached to the cyst wall. Each scolex has four circular suckers and two rows of hooks on a rostellum. The hook lengths are 147 to 165 µm and 87 to 125 µm.The results show that C. cerebralis was a major cause of the nervous manifestations of cenurosis in clinically affected sheep.Proceeding
Značaj ekto i endoparazita u patologiji autohtonih vrsta domaćih životinja na zajedničkim pašnjacima
No full textInfekcije parazitima predstavljaju značajan zdravstveni problem kod životinja, posebno držanih na zajedničkim pašnjacima, gde se mešaju različite vrste životinja. Uzrokuju ih protozoe, helminti i artropode. Kolika je prevalenca i kojih sve vrsta parazita trenutno ima u Srbiji teško je reći, s obzirom na to da se ovakva istraživanja vrše sporadično. To se pre svega odnosi na autohtone vrste i rase domaćih životinja, koje nisu obuhvaćane uobičajenim merama kontrole parazita. Na osnovu povremenih ispitivanja može se, ipak, steći slika o najčešćim parazitskim infekcijama koje ugrožavaju ove vrste životinja i one će detaljnije biti opisane u daljem tekstu.Parasitic infections are a significant health problem in animals, especially kept in common pastures, where they mix different types of animals. They are caused by protozoa, helminths and arthropods. What is the prevalence and what kinds of parasites are there currently in Serbia it is difficult to say, given that such research is being carried out sporadically. This primarily refers to autochthonous species and domestic breeds animals that are not covered by the usual parasite control measures. On the basis of occasional tests, however, it is possible to get a picture of the most common parasitic infections that threaten these types of animals and they will be described in more detail below.Zbornik predavanja. -
Predavanje po poziv
Genetic analysis reveals multiple intergenic region and central variable region in the African swine fever virus variants circulating in Serbia
No full textThis study provides the frst comprehensive report on the molecular characteristics of African swine fever virus (ASFV) variants in Serbia between 2019 and 2022. Since its frst observation in July 2019, the disease has been found in wild boar and domestic swine. The study involved the analysis of 95 ASFV-positive samples collected from 12 infected administrative districts in Serbia. Partial four genomic regions were genetically characterized, including B646L, E183L, B602L, and the intergenic region (IGR) between the I73R-I329L genes. The results of the study suggest that multiple ASFV strains belonging to genotype II are circulating in Serbia, as evidenced by the analysis of the IGR between I73R-I329L genes that showed the most diferences. Furthermore, the phylogenetic analysis of the B602L gene showed three diferent clades within the CVR I group of ASFV strains. Regarding the IGR, 98.4% were grouped into IGR II, with only one positive sample grouped into the IGR III group. These fndings provide essential insights into the molecular characteristics of ASFV variants in Serbia and contribute to the knowledge of circulating strains of ASFV in Europe. However, further research is necessary to gain a better understanding of ASFV spread and evolutio
Improvement of quality and safety of freshwater fish – one health approach
No full textAkvakultura je grana privrede koja je doživela orgroman globalni rast u poslednjih nekoliko
decenija. Na dalji razvoj akvakulture će svakako uticati tržište, mišljenje potrošača,
a posebno njihova zabrinutost vezana za bezbednost proizvedene ribe, ali i za uticaj
proizvodnje ribe na životnu sredinu. Riba se smatra neizostavnom namirnicom u zdravoj
ishrani, ali treba imati u vidu da mnogobrojni hazardi, kao što su mikrobiološki, hemijski
i drugi mogu dospeti u ribu u toku celog procesa proizvodnje. Imajuću sve u vidu veoma
je važno poznavanje i razumevanje distribucije i uloge ovih hazarda duž celog procesa
proizvodnje, a prvenstveno je važno njihovo sagledavanje sa aspekta jednog zdravlja. Za
nesmetani razvoj akvakulture je od ogromnog značaja primena dobre ribarske prakse i
strogo pridržavanje i primena postojećih zakonskih propisa. Da bi se obezbedio kvalitet
i pre svega osigurala bezbednost proizvedene ribe neophodno je uvođenje sistema
menadžmenta kvaliteta. Ogroman značaj takođe ima primena biosigurnosnih mera u
primarnoj proizvodnji. Multidiscipliniarni pistup, kao i udruživanje različitih sektora po
prinicipima Jednog zdravlja može dovesti do povećanja efikasnosti proizvodnje u akvakulturi
sa minimalnim negativnim efektom na životnu sredinu. Ovakav pristup je neophodan za
održivi razvoja slatkovodne akvakulture, a glavni cilj je da se osigura proizvodnja bezbedne
hrane što je preduslov za nesmetanu trgovinu ribom.Aquaculture is an economic sector that has experienced tremendous global growth in the
last few decades. The further development of aquaculture will certainly be influenced
by the market, the opinion of consumers, and especially by their concern regarding the
safety of the produced fish, but also the impact of fish production on the environment.
Fish is considered an important food in a healthy diet, but it should be borne in mind
that numerous hazards, such as microbiological, chemical and others, can get into the
fish during the entire production process. Having all above mentioned in mind, it is very
important to know and understand the distribution and role of these hazards along the
entire production process, and it is primarily important to consider them from the aspect
of one’s health. For the regular development of aquaculture, the application of good
fishing practices and the strict adherence and application of existing regulations are of
great importance. In order to ensure the quality and the most important the safety of the
produced fish, it is necessary to introduce a quality management system. The application of
biosecurity measures in primary production is also of great importance. A multidisciplinary
approach, as well as the cooperation of different sectors according to the principles of
One Health, can lead to an increase in the efficiency of production in aquaculture with a
minimal negative effect on the environment. This approach is necessary for the sustainable
development of freshwater aquaculture, and the main goal is to ensure the production of
safe food, which is a prerequisite for an unhindered fish trade
The role of protozoal infections in the cause of neonatal enteropathies of piglets
No full textIntroduction: Neonatal enteropathies of piglets were recognized as a serious problem of the
modern pig industry. Today it is known that it is a multicausal disease caused by numerous
agents of bacterial, viral and parasitic etiology (Jacobson, 2022) The aim of our work is to
point out the importance of parasitic infections in this disease.
Materials and Methods: In period 2017-2020 a total of 337 pigs, 2-21 weeks old, were
exanimate. After routine necropsy, parasitological, bacteriological and viral evaluations were
performed, as required to establish the cause of disease in each pig. To parasitological
examination we used faecal swab, gut contents and a mucous scrape of gut which we
examined with Sheathers sugar flotation and in direct smears stained with Gimsa strain.
Results: During our examination Eimeria sp. were detected at two week old piglet at 4.22%,
Ispsora suis at 2-4 week old piglet at 12,34% and cryptosporidial organisms were detected in
the microvillus brush border in the intestine of the pigs ranged from 7-12 weeks old at
61,36%. Based on the time of onset of neonatal enteropathies, Cryptosporidium sp. was
confirmed as the main parasitic agent of its occurrence. These results are similar to our
previous research (Pavlovic et al., 2007)
Conclusion: The role of infection with criptosporidia in the occurrence of neonatal
enteropathies in piglets, because of the multiple agents, which cause this disease, has not been
fully explained, but our examination indicated that cryptosporidia might act, either in itself or
in concert with other agents to induce or exacerbate clinical disease
Organic acids as solvents for leaching PAHs out of impregnated wood
No full textPolycyclic aromatic hydrocarbons (PAHs) are a group of hydrophobic organic
compounds, which are ubiquitous in the environment. Some of them exhibit toxicity,
mutagenicity, and carcinogenicity [1]. There is a high PAH content in creosote impregnated
wood [2].
Creosote impregnated wood which was used and discarded becomes hazardous waste.
Concentration of PAHs in used impregnated wood can be reduced by leaching with suitable
solvent systems. Thus, the aim of this study was to compare different organic acids solvent
systems for leaching PAHs out of impregnated wood to obtain non-hazardous waste.
Leaching tests on impregnated wood were performed with 15 % water solution of urea,
concentrated formic acid, 15 % solution of urea in formic acid and glacial acetic acid [3].
Concentration of PAHs in extracts was measured by GC-FID technique. Polycyclic aromatic
hydrocarbons that were determined are naphthalene, 1-methylnaphthalene, acenaphthene,
fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo[a] anthracene and
chrysene.Poster prezentacije, Kladovo, 4-7th June 202
Coccidiosis of pet cats in Belgrade area during 2021
No full textFeline coccidiosis is a disease of mostly young animals and regardless of the large number of
infected animals, the disease rarely appears. The causative agents are coccidia from the genus
Cystoisospora (Isospora) (C. felis, C. bigemina and C. rvolta) and Eimeridae (Eimeria felina
and E. cati). Infection is produced orally by coccidia oocysts. Parasites are located in the
mucosa of the small intestine. Infections pass in most cases asymptomatic. In severe
infections, in kittens long-term bloody diarrhea occurs, there is a general weakness and loss of
appetite, and the animals lose weight quickly. Depending on the severity of the infection,
death is possible, especially with infections with Cystoisospora felis. In animals that have
recovered, the symptoms subside 5 to 7 days. During 2021 we examined feces of 81 pets cats
that had symptoms of dysentery, diarrhea, weight loss, fainting and abdominal pain. It usually
occurs in young animals. A fecal sample is examined for oocysts using fecal flotation
(saturated salt or sucrose solutions). Speciation should be undertaken, and in puppies this may
require artificial sporulation of the oocysts with potassium dichromate. Infection with
Cystoisospora felis we established in 22.13%, with C. rvolta in 3.27% and with Eimeria cati
in 11.18% of examined cats.The proceeding boo
Program kontrole kocidioze ovaca u lancu proizvodnje od odgajivača do potrošača
No full textKokcidioza ovaca se javlja u akutnom i hroničnom toku, sa velikim morbiditetom, ređa klinička manifestacija koje se javlja kod jagnjadi. Osnovni cilj programa kontrole kokcidioze ovaca jeste podizanje zdravstvenog statusa ovaca u Republici Srbiji. Sprečavanjem pojave i širenja kocidioze preduzimanjem određenih biotehničkih i zdravstvenih mera koje imaju ulogu da smanje prevalenciju parazita, postiže se veći prirast i bolji proizvodni rezultati ovaca. Primena ovog integrisanog koncepta kontrole infekcije parazitima zahteva sistematsko praćenje infekcije na farmama, pre i posle primenjenih mera i zahteva uključivanje svih relevantnih subjekata, prvenstveno veterinarske službe, počev od farmi, pa do radnika sa životinjama
A multi gene-approach genotyping method identifies 24 genetic clusters within the genotype II-European African swine fever viruses circulating from 2007 to 2022
No full textIntroductionAfrican swine fever (ASF) is a contagious viral disease of pigs and wild boar that poses a major threat to the global swine industry. The genotype II African swine fever virus (ASFV) entered the European Union (EU) in 2014 and since then fourteen countries have been affected, Italy and North Macedonia being the last in 2022. While whole genome sequencing remains the gold standard for the identification of new genetic markers, sequencing of multiple loci with significant variations could be used as a rapid and cost-effective alternative to track outbreaks and study disease evolution in endemic areas. Materials and methodsTo further our understanding of the epidemiology and spread of ASFV in Europe, 382 isolates collected during 2007 to 2022 were sequenced. The study was initially performed by sequencing the central variable region (CVR), the intergenic region (IGR) between the I73R and I329L genes and the O174L and K145R genes. For further discrimination, two new PCRs were designed to amplify the IGR between the 9R and 10R genes of the multigene family 505 (MGF505) and the IGR between the I329L and I215L genes. The sequences obtained were compared with genotype II isolates from Europe and Asia. ResultsThe combination of the results obtained by sequencing these variable regions allowed to differentiate the European II-ASFV genotypes into 24 different groups. In addition, the SNP identified in the IGR I329L-I215L region, not previously described, grouped the viruses from North Macedonia that caused the 2022 outbreaks with viruses from Romania, Bulgaria, Serbia and Greece, differentiating from other genotype II isolates present in Europe and Asia. Furthermore, tandem repeat sequence (TRS) within the 9R-10R genes of the multigene family 505 (MGF505) revealed eight different variants circulating. DiscussionThese findings describe a new multi-gene approach sequencing method that can be used in routine genotyping to determine the origin of new introductions in ASF-free areas and track infection dynamics in endemic areas
Bolesti jetre svinja u intenzivnom uzgoju
No full textBolesti jetre kod svinja su prisutne i ispoljavaju se u velikim aglomeracijama. Prema literaturnim
podacima kao uzroci oboljenja jetre se smatra jednolična i deficitarna ishrana, alimentarni i
bakterijski toksini , parazitke infekcije i neadekvatni zoohigijenski uslovi. Promene na jetri ne
ispoljavaju vidljive kliničke simptome. U postavljanju dijagnoze koristi se analiza biohemisjkih
parametarta. U ovom predavanju smo izložili rezultate biohemuisjske anlize nerastova koji se
koriste u repordukciji sa komercijalnih farmi. Koristili smo sledeće parametre: fosfor, ukupne
proteine, urea, kreatinin, AST. Statiststički značajne razlike su ustanovljene kod sledećih
parametara: fosfor, ukupni proteini, urea i AST. Cilj ovog predavanja je da ukaže na značajnost
biohemijskih paramtera jetre u zdravtvenoj zaštiti svinja