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    Herbicide residue concentrations in the waters of the DTD channel in 2024 and 2025

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    Vojvodina is an agricultural region where numerous plant protection products are used. Also in Vojvodina there is a large network of melioration canals (navigable in length of about 600 km) which are more or less in use, but they are certainly places where agrochemicals accumulate. The aim of the work is to determine how much the application of plant protection products in agriculture affects the accumulation of these compounds in the surface waters of the canal network of Vojvodina. The paper presents the results of testing the presence of 41 herbicides (Mitić et all, Environments 2025, 12, 246) in the waters of the Danube-Tisa Danube canal system. 2024. Selected herbicides are still in use. In 2024, 100 water samples taken along the entire length of the Bogojevo-Bečej canal were examined, while in 2025, 90 samples were examined from the Vrbas-Bezdan, Odžaci- Sombor and Karavukovo-Bački Petrovac canals (Image 1). The samples were taken in 1 L glass bottles, preserved with formic acid and stored at +4 0C until analysis. Extraction was performed using solid-phase disk extraction on CDC Analytical EmporeTM disks (Catalog number: 2215- C-18, lot 320005C) with a diameter of 47 mm. The herbicides were eluted from the disk with two portions of 5 ml of acetonitrile each, the obtained extract was evaporated in a stream of nitrogen and reconstituted with 1 ml of acetonitrile. The analysis was performed on HPLC-MS/MS VANQUISH CORE/TSQ Quantis Plus and on GC-MS/MS system Thermo Trace 1300/ TSQ 9000 with confirmation of the identity of compounds based on the formed ions/daughter ions. The certified standards from Riedel-de-Haen were used for quantification. The results showed that s-metolachlor was found in 100% and 97% of the water samples. In 2024 and 2025, the average concentrations of smetolachlor were 0.037 μg/L and 0.091 μg/L, respectively. Trebuthylazine was also found in 97% of samples in both years at concentrations of 0.0108 μg/L and 0.388 μg/L. In this study, among others, 9 Sulfonyl-urea herbicides, which are used in extremely low doses, were tested. The results 128 showed that in 2024 and 2025, nicosulfuron was the most abundant and was found in 76% and 36% of water samples, respectively. The second most common is amidosulfuron, which was found in water in 55% and 46% of the tested samples, respectively. Concentrations of sulfonyl urea herbicides and other compounds listed here were also tested in agricultural soil. It has been established that in 2024 amidosulfuron, nicosulfuron, smetolachlor, triflusulfuron-meil, foramsulfuron, terbuthylazine and others ARE detected in significant concentrations in the soil, namely: 0.197 μg/L, 0.206 μg/L, 0.270 μg/L, 145.47 μg/L, 10.358 μg/L, 30,173 μg/L, respectively (Mitić et all, Environments 2025, 12, 246

    Potentially toxic elements in agricultural soils : in vitro bioaccessibility and health risk

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    Agricultural soil is frequently exposed to various pollution sources due to frequent use of agrochemicals and the proximity of anthropogenic sources. Fertilizers, pesticides, irrigation practices, industrial activities, and especially illegal waste disposals may all contribute to increased pollution of agricultural soil. Once present in the environment, these pollutants may migrate into water, air, and agricultural products, leading to human exposure and potential health risks. Therefore, monitoring and assessing the behaviour of pollutants in the agricultural soil is essential to mitigate pollution and protect human health. Within the framework of several complementary experiments, we investigated the occurrence, oral and inhalation bioaccessibility and human health risk of potentially toxic elements (PTEs) in different agricultural areas in Serbia. The study included vineyard soils from Central and South Serbia and soils collected in the vicinity of illegal landfills in Vojvodina Province and Central Serbia. Pseudo-total concentrations of PTEs were determined using Inductively Coupled Plasma–Optical Emission Spectroscopy (ICPOES). To better understand the real human exposure, in vitro oral and inhalational bioaccessibility were performed. The Unified BARGE Method (UBM) was applied to simulate the gastrointestinal tract, while the simulated lung fluid (SLF) procedure was used to assess the PTE bioaccessibility in the lungs. The results consistently indicated that oral exposure is the most relevant pathway for human health risk associated with PTE in soil. In vineyard soils, Sr, Cu, and Co showed the highest bioaccessibility in the gastrointestinal tract, followed by Mn, Ni, and Zn. Cumulative health risk assessment demonstrated that pseudo-total Ni, Cr, 185 Co, and Mn concentrations contributed most significantly to the health risk of workers in the vineyards. However, when bioaccessible (oral and inhalational) fractions were considered, the assessed risk did not exceed threshold values (THQ<1), suggesting no increased risk for workers in the investigated vineyards. By contrast, in agricultural areas located near uncontrolled waste dumps, higher PTE concentrations in the soil were determined. The oral bioaccessibility of Cu was particularly elevated (40.54% of the total concentration), while other investigated PTEs exhibited slightly lower values. Human health risk assessment revealed an increased concern for local agricultural workers, with results pointing to a moderate carcinogenic risk based on both pseudo-total (2.60 × 10⁻⁵) and bioaccessible (1.58 × 10⁻⁶) PTE concentrations. These findings emphasize the importance of including bioaccessibility data in exposure and risk modeling, as the actual internal dose is often lower than the pseudo-total PTE concentrations in soil. Assessing only pseudo-total concentrations may lead to an overestimation of the actual exposure and health risk, especially for PTEs. Therefore, incorporating bioaccessibility data is essential for accurate risk characterization and for prioritizing remediation actions in polluted agricultural areas

    Microbial community structure in landfill soils : Case study in Serbia

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    Microorganisms are vital for soil health and demonstrate an adaptive capacity to environmental change, with their role in plastic biodegradation increasingly recognized. Main purpose of this case study was to analyse bacterial and fungal communities in soils where high numbers of microplastics are expected. Soil samples were collected from three land use types: active landfill, remediated landfill, and agricultural soil. From each field, we took 3 replicates and isolated all DNA from soil samples of the top soil layer (0 - 10cm). Microbial community profiling was performed using Illumina amplicon sequencing, making use of a Novaseq (2x300 bp, paired-end), targeting the V3-V4 fragment of the 16S rRNA gene (bacteria), and ITS2 rDNA region (fungi). Sequencing analysis showed shifts in microbial community structure across the tested soil types. In the agricultural soil (control), the bacterial community was dominated by Pseudomonadota (>50%) and Actinomycetota (20–25%). The active landfill exhibited a significant reduction in both species, with a strong increase in Bacteroidota. This change in community structure was proven by a PERMANOVA analysis (p<0,001) showing a statistical difference in microbial community in between the two land types. In the remediated landfill the relative abundance of Pseudomonadota was higher than in the active landfill but still lower than in the control. This indicates that the long-term effects of landfill continue to prevent a full return of microbial communities to the structure observed in the agricultural soil, served as control. However, we do acknowledge that the effects could also be soil dependent and should be evaluated further with information regarding nutrient status, organic matter content, microplastic content and soil texture. These findings highlight the lasting impact of land use and remediation efforts on soil microbial communities, aligning with existing results on environmental stress and community composition

    Managing Microplastics for Sustainable Agriculture : Legal and Policy Perspectives from the EU and Serbia

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    Management of microplastics in agriculture is not an isolated environmental issue but a key area for achieving multiple goals of the 2030 Agenda – from resource protection and the attainment of sustainable agriculture to ensuring food safety and preserving ecosystems. This paper examines existing legislation, regulatory frameworks, and policy measures addressing plastic management, with a particular focus on microplastic, within the framework of sustainable agriculture, focusing on the European Union (EU) and the Republic of Serbia. Special attention is given to sectoral approaches targeting microplastic reduction in water, soil, air, and food systems, as well as relevant directives on waste, packaging, and environmental quality standards. The research concludes that achieving sustainable agriculture in both the EU and Serbia requires the development of a coherent and comprehensive regulatory framework that explicitly governs microplastic generation, use, and mitigation

    Ecological, economic and social dimensions of circular business models

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    The need for new business models based on the principles of sustainability and efficient resource use in modern economic conditions is becoming increasingly evident. These business models strive to eliminate the fundamental weaknesses of the linear economy, such as uncontrolled extraction of natural resources, irreversible waste generation, and their inherently weak management potential. At the same time, they must meet increasingly demanding market needs, both on a national and global level. Depending on the key characteristics and goals of circular business models based on: a) recycling and reuse, b) design for sustainability, c) services, d) closed-loop supply chain processes, e) digitalization, and f) educational and consultancy nature, the study considers their impact on the ecological, economic and social dimensions of sustainable business. It concludes that recycling and closed-loop supply chain circular business models most significantly advance ecological goals but may carry moderate economic challenges. On the other hand, service-oriented models and digitalization bring high economic benefits, with moderate ecological impact

    Primena modela kontinuiranog unapređivanja poslovnih procesa i njihov uticaj na konkurentnost preduzeća

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    Sve izraženija globalna konkurencija primorava organizacije da implementiraju nove organizacione i proizvodne tehnike kako bi se takmičile i unapredile svoju konkurentsku poziciju. Korišćenje modela, alata i tehnika za kontinuirano unapređenje poslovnih procesa postala je prilika i efikasna strategija da se to postigne. U tom kontekstu, cilj ovog rada je da se prikaže uticaj modela kontinuiranog unapređenja poslovnih procesa na poboljšanje ukupnih performansi organizacije, a samim tim i bolju konkurentsku poziciju. Formulisana je hipoteza koja glasi kontinuirano unapređenje poslovnih procesa direktno utiče na konkurentnost preduzeća. Formulisana hipoteza ispitana je na preduzećima industrije mleka u Republici Srbiji. Istraživanje je sprovedeno na stratifikovanom uzorku kojim se obezbedilo da zbirni podaci imaju adekvatan značaj, odnosno reprezentativnost. Pomoću vođenog intervijua i na osnovu Delphi metode prikupljeni su podaci koji su potom obrađeni na osnovu Spirmanove korelacije ranga kao neparametarske alternative Pirsonove korelacije. Rezultati istraživanja koji su predstavljeni u ovom radu idu u prilog potvrđivanju postavljene hipoteze da se kontinuiranim unapređenjem poslovnih procesa direktno utiče na konkurentnost preduzeća

    Cutting-Edge Methods for Identifying Microplastics in Agricultural Environments

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    The growing presence of microplastics in the environment and food chain raises concerns about their impact on ecosystems and health. Accurate detection in water, soil, feed, and biological tissues is essential for assessing contamination levels and risks. Modern instrumental techniques help identify microplastics, providing insights into their composition, size, and morphology. Key analytical methods include Fourier Transform Infrared Spectroscopy (FTIR), Raman Spectroscopy, and Pyrolysis-Gas Chromatography/Mass Spectrometry (Py-GC/MS). FTIR and Raman allow non-destructive polymer identification, while Py-GC/MS enables precise chemical characterization. Advanced imaging techniques like Scanning Electron Microscopy (SEM) and Flow Cytometry further enhance detection in complex samples. Despite advancements, challenges remain in standardizing methods, ensuring reproducibility, and distinguishing microplastics from other particles. Further research is needed to refine detection, improve automation, and lower costs. Reliable methods support regulations and help mitigate microplastic pollution. As part of ECO(RE)ACT—Enhancing Climate Resilience through Reducing Microplastic Pollution in Agricultural Ecosystems—FTIR spectroscopy will be key for detecting microplastics in irrigation water, soil, and feed, improving understanding of their impact on agriculture and food safety. Standardized protocols and advanced tools will enhance detection accuracy and support mitigation efforts.Agriculture for Better Tomorrow, Innovation and entrepreneurship, 60th Croatian and 20th International Symposium on Agriculture, June 1 – 6 2025, Bol, Brač Island, Croati

    Challenges in Circular Economy Implementation: Insights from North Macedonia and Serbia

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    The focus of the Green Deal is to position Europe as a global leader in the circular economy. To implement the principles of the circular economy, the European Commission adopted the "EU Action Plan for the Circular Economy" (2015) and the "New Circular Economy Action Plan for a Cleaner and More Competitive Europe" (2020). In 2022, it published a set of two packages of proposals for encouraging the circular economy in achieving the sustainable development goals by 2030. These proposals include various practices such as industrial symbiosis, as a key enabling component of the circular economy, which involves the exchange of resources, energy, and by-products among industries, to improve efficiency and sustainability. The Western Balkans region faces significant barriers, both technical and non-technical, in the implementation of the circular economy. The aim of this paper is to identify the main obstacles in the practice of implementing the circular economy, using examples from two Western Balkans countries, namely North Macedonia and Serbia. The collected data is based on a systematic review of theoretical frameworks and empirical verification of the results. In addition to the introduction, the paper consists of three sections. The first section of the paper presents the key barriers for the circular economy implementation in North Macedonia, while the second section analyzes the same barriers in Serbia. The third section analyzes the current state of circular economy implementation in the respective countries based on Eurostat's data categories. Finally, the authors’ conclusions and insights are given.The third international scientific conference GIRR 2025 “Global challenges through the prism of rural development in the sector of agriculture and tourism“, May 9th 2025, Šabac, Serbi

    Impact of Sustainable Agricultural Practices on Early Potato Yield Components

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    Soil and plant covering methods offer sustainable agricultural benefits by improving soil health, water use efficiency, and reducing chemical inputs. A three-year split-plot experiment (2019–2021) evaluated the impact of various covering techniques on early potato production using two varieties (Cleopatra and Riviera). Treatments included control, plastic mulch, agrotextile, low tunnels, and their combinations. The study assessed stem number, number of tubers per plant (including per stem), average tuber mass, tuber mass per plant, and tuber yield. Results demonstrate the significant positive impact of covering methods on early potato yield. The combination of agrotextile and low tunnels resulted in the highest increases, improving key yield components like tuber number and mass per plant. While varietal differences existed, the covering methods’ influence was paramount. These findings offer a basis for optimizing early potato production for higher yields and quality. Future research should investigate the economic viability of these methods and explore organic alternatives to plastic coverings for sustainable agriculture

    Repellent efficacy of icaridin on adult Ixodes ricinus ticks (Acari: Ixodidae)

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    Chemical repellents, particularly those based on synthetic and bioactive compounds, remain a primary means of tick bite prevention; however, standardized efficacy evaluations are essential. Icaridin (picaridin), a synthetic repellent structurally related to natural compounds, has demonstrated broad-spectrum efficacy against various arthropods, warranting further assessment under controlled laboratory conditions as well as in natural environments. This study was conducted under laboratory conditions to assess the repellent efficacy of a preparation containing 15% icaridin as a function of time against adult Ixodes ricinus, both male and female. Repellence was calculated using two types of tests, each performed in five replicates: (1) application of the preparation to filter paper in Petri dishes and (2) direct application to the skin of the examiner‘s forearm. The aim of this study was to compare the repellent efficacy of the preparation regarding the type of the experiment and the elapsed time, from the moment of application up to eight hours. The average repellence during the experiment in Petri dishes was 96.8% for I. ricinus females and 98.4% for males. During the tests conducted on the examiner‘s skin, the repellent efficacy was 95.11% for females and 94.11% for males. While efficacy remained high under all test conditions, further investigations should assess formulation stability, field performance, and user safety under diverse environmental exposures. Studies designed to precisely calculate repellent efficacy require continuous methodological improvements to create economically viable and widely available commercial preparations that are harmless to human health, especially to children. Furthermore, standardized and verified methodologies for repellent testing should be continuously refined to improve reliability, facilitate regulatory approvals, and ensure optimal protection against ticks and other arthropods, which are proven significant vectors and reservoirs of various pathogens.15th CASEE Conference "Green transitions in agriculture, forestry, veterinary medicine and food systems under a changing climate", 25th – 27th June 2025, organized by the University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbi

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