Ehime University Institutional Repository
Not a member yet
5403 research outputs found
Sort by
A Consideration on the "Daihannyakyo 大般若経" of the Muryoji Temple in Imabari-city
Get PDFdepartmental bulletin pape
愛媛大学図書館報「図書館だより」第85号
Get PDF本と私 / 山本晴康必見!! 知って得する図書館活用方法特集愛媛大学図書館企画展(三輪田米山)2008年ご案内図書館からのお知らせ図書館日誌othe
An atempt to use fresh water bivalve Corbicula for teaching materials
Get PDFdepartmental bulletin pape
A Study to the Way of Thinking of Multidisciplinary Habilitation Staffs (No.14) : Support of Very Low Birth Weight Children in Their School and Kindergarten
Get PDFdepartmental bulletin pape
Effects of polyamines on proliferation and IgM productivity of human-human hybridoma, HB4C5 cells
Get PDFImmunoglobulin production stimulating activity of polyamines was investigated. Spermidine, thermine and triethylenetetraamine (TETA) stimulated IgM production of human-human hybridoma, HB4C5 cells under serum-free condition. IgM production of HB4C5 cells was accelerated 5.9-, 5.3-, and 3.7-fold by spermidine at 4.5 mM, thermine at 2 mM and TETA at 2.5 mM, respectively. However, putrescine did not enhance IgM production. Spermidine enhanced IgM productivity of the hybridoma cells in spite of its growth suppression activity. TETA also inhibited cell proliferation and the effect on the acceleration of IgM productivity disappeared during 5 days because of its cytotoxicity. On the other hand, thermine facilitated IgM productivity of the hybridoma cells without growth suppression. The laser confocal microscopic analysis revealed that IgM content inside HB4C5 cells was increased by thermine. This result suggests that thermine facilitates IgM synthesis in hybridoma cells.journal articl