Repositorio académico de acceso abierto (Univ. Autonóma de Chile)
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Screen time is negatively associated with sleep quality and duration only in insufficiently active adolescents: A Brazilian cross-sectional school-based study
No full textBackground: Time spent on screen devices affects sleep quality and duration leading to several health impairments in youth. Although physical activity (PA) benefits sleep patterns and decreases screen time in adolescents, it is unclear whether the relationship between sleep quality/duration and screen time could be influenced by PA levels. Objective: To analyze the association between sleep quality and duration with screen time in Brazilian adolescents according PA levels. Methods: The sample included 1010 adolescents aged 13.2 ± 2.4 years (n = 556 females − 55 % of the sample). Sleep quality and sleep duration, and PA were assessed by Mini Sleep and Baecke questionnaires, respectively. Participants in the highest quartile were classified as physically active. Screen time was analyzed by the self-reported number of hours spent on different screen devices (i.e., television, computer, videogame, and cellphone/tablet). Participants in the highest tertile were classified as having high screen time. Sex, age, and body mass index were considered covariates in binary logistic regression models. Results: Poor sleep quality was observed in 52.3 % of the sample, whereas 46.6 % reported sleeping less than eight hours/day. High screen time was associated with poor sleep quality (OR = 1.45; 95 %CI = 1.01–2.12) and insufficient sleep duration (OR = 1.52; 95 %CI = 1.01–2.03) in adolescents insufficiently active. There were no associations between screen time and sleep parameters in active adolescents. Conclusion: High screen time was associated with poor sleep quality and insufficient sleep duration only in insufficiently active adolescents. These results suggest that high PA levels may contribute to improving sleep patterns in pediatric population. © 2023Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES; Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq, (305886/2022-3, 308954/2021-1)Universidad Autónoma de Chil
Microplastics in marine ecosystems: A comprehensive review of biological and ecological implications and its mitigation approach using nanotechnology for the sustainable environment
No full textMicroplastic contamination has rapidly become a serious environmental issue, threatening marine ecosystems and human health. This review aims to not only understand the distribution, impacts, and transfer mechanisms of microplastic contamination but also to explore potential solutions for mitigating its widespread impact. This review encompasses the categorisation, origins, and worldwide prevalence of microplastics and methodically navigates the complicated structure of microplastics. Understanding the sources of minute plastic particles infiltrating water bodies worldwide is critical for successful removal. The presence and accumulation of microplastics has far reaching negative impacts on various marine creatures, eventually extending its implications to human health. Microplastics are known to affect the metabolic activities and the survival of microbial communities, phytoplankton, zooplankton, and fauna present in marine environments. Moreover, these microplastics cause developmental abnormalities, endocrine disruption, and several metabolic disorders in humans. These microplastics accumulates in aquatic environments through trophic transfer mechanisms and biomagnification, thereby disrupting the delicate balance of these ecosystems. The review also addresses the tactics for minimising the widespread impact of microplastics by suggesting practical alternatives. These include increasing public awareness, fostering international cooperation, developing novel cleanup solutions, and encouraging the use of environment-friendly materials. In conclusion, this review examines the sources and prevalence of microplastic contamination in marine environment, its impacts on living organisms and ecosystems. It also proposes various sustainable strategies to mitigate the problem of microplastics pollution. Also, the current challenges associated with the mitigation of these pollutants have been discussed and addressing these challenges require immediate and collective action for restoring the balance in marine ecosystems. © 2024 Elsevier Inc
Photocatalytic activity of zinc oxide nanorods incorporated graphitic carbon nitride catalyst
No full textBackground: Photocatalysts are user-friendly and serve as compatible materials for degrading industrial dye pollutants. This study utilizes zinc oxide/graphitic carbon nitride (ZnO/g-C3N4) nanocomposites against degrading methylene blue (MB). Methods: The hydrothermal method assisted sonication technique was used to fabricate the ZnO/g-C3N4 composite with varying ratios of ZnO/g-C3N4 (1:0.25, 1:0.50, 1:1). The synthesized materials have undergone various sophisticated techniques for finding their physiochemical properties and have been utilized for photodegradation activities. Significant findings: The characterized results exhibit that the nanoflakes of g-C3N4 were covered with nanorods of zinc oxide when observed through scanning electron microscopy (SEM). Furthermore, the X-ray diffraction (XRD) studies demonstrate that the ZnO/g-C3N4 material was successfully synthesized. The X-ray photoelectron spectra (XPS) and Fourier-transform infrared (FTIR) spectra revealed the present oxidation states and chemical bonding of the materials. The photocatalytic activity results demonstrated that the concentration of ZnO molar ratio in varying g-C3N4 significantly affected the decomposition performance. The ZnO/g-C3N4 (1:0.50) presented a higher rate of degradation, reaching 92% at 120 minutes under UV light and 65% at 240 minutes under visible light irradiation. This could be explained by the mechanism that follows the separation of charge carriers, thereby producing hydroxyl radicals for the effective degradation of MB pollutants. © 2023 Taiwan Institute of Chemical EngineersKing Saud University, KS
Targeting CCR3 with antagonist SB 328437 sensitizes 5‑fluorouracil‑resistant gastric cancer cells: Experimental evidence and computational insights
No full textGastric cancer (GC) ranks fifth globally in cancer diag‑ noses and third for cancer‑related deaths. Chemotherapy with 5‑fluorouracil (5‑FU), a primary treatment, faces challenges due to the development of chemoresistance. Tumor microenviron‑ ment factors, including C‑C motif chemokine receptor 3 (CCR3), can contribute to chemoresistance. The present study evaluated the effect of CCR3 receptor inhibition using the antagonist SB 328437 and the molecular dynamics of this interaction on resis‑ tance to 5‑FU in gastric cancer cells. The 5‑FU‑resistant AGS cell line (AGS R‑5FU) demonstrated notable tolerance to higher concentrations of 5‑FU, with a 2.6‑fold increase compared with the parental AGS cell line. Furthermore, the mRNA expression levels of thymidylate synthase (TS), a molecular marker for 5‑FU resistance, were significantly elevated in AGS R‑5FU cells. CCR3 was shown to be expressed at significantly higher levels in these resistant cells. Combining SB 328437 with 5‑FU resulted in a significant decrease in cell viability, particularly at higher concentrations of 5‑FU. Furthermore, when SB 328437 was combined with 5‑FU at a high concentration, the relative mRNA expression levels of CCR3 and TS decreased significantly. Computational analysis of CCR3 demonstrated dynamic conformational changes, especially in extracellular loop 2 region, which indicated potential alterations in ligand recognition. Docking simulations demonstrated that SB 328437 bound to the allosteric site of CCR3, inducing a conformational change in ECL2 and hindering ligand recognition. The present study provides comprehensive information on the molecular and structural aspects of 5‑FU resistance and CCR3 modula‑ tion, highlighting the potential for therapeutic application of SB 328437 in GC treatment. © 2024 Spandidos Publications. All rights reserved.Universidad de La Frontera, UFRO; National Research and Development Agency; National Doctoral Scholarship, (21222011); Fondo de Fomento al Desarrollo Científico y Tecnológico, FONDEF, (ID21I10027); Fondo de Fomento al Desarrollo Científico y Tecnológico, FONDEF; Instituto Milenio en Inmunología e Inmunoterapia, MIII, (ICN09_016/ICN 2021_045); Instituto Milenio en Inmunología e Inmunoterapia, MIII; Fondo Nacional de Desarrollo Científico y Tecnológico, FONDECYT, (3210629, 1210440); Fondo Nacional de Desarrollo Científico y Tecnológico, FONDECYTUniversidad Autónoma de Chil
Cooperação intergovernamental: classificação, características e formatos à luz da experiência brasileira
No full textA cooperação e articulação intergovernamental tem se apresentado como alternativas para o fortalecimento das capacidades governamentais. No Brasil, a adoção de um modelo descentralizado desencadeou, a partir do final da década de 90, o surgimento de tais mecanismos em diversos setores. Com o intuito de contribuir com um arcabouço teórico e conceitual acerca da temática em questão, o artigo apresenta as principais formas de cooperação e articulação intergovernamental e examina como esses instrumentos tem se desenvolvido no Brasil. A partir de pesquisa bibliográfica de cunho qualitativo e exploratório, com ênfase na análise de conteúdo e documental, constatou-se o avanço da governança territorial no Brasil, embora muitas das iniciativas identificadas estejam em processo de experimentação e aprendizado.  
Considerations On The Use Of Xenogenous Bone Blocks In Implantology: A Case Report
No full textIn implantology, an increase in the bone thickness of the alveolar ridge is often associated with the use of autogenous bone, which is considered to be the gold standard. The aim of the present study was to report the clinical case of a patient with abnormal bone thickness in the alveolar ridge, causing severe esthetical damage, and the use of xenogeneic block bone grafts. A female, 43-year old patient exhibited a considerable bone defect in the region of teeth 11 (absent), although there was sufficient native bone available for the implantation. The patients treatment involved positioning the implant and performing a block bone graft using bone of bovine origin. The post-operative period passed without complications. The post-operative clinical examination confirmed the correction of the pre-existing esthetic defect and the health of the soft tissues involved. The tomographic examination 180 days post-surgery also confirmed an excellent increase in thickness. The bovine block bone graft used in the present case was shown to be viable in terms of correcting esthetic defects when there is sufficient native bone available for the implantation
EL DESARROLLO ECONÓMICO LOCAL Y LOS PARQUES INDUSTRIALES: EL CASO DEL “POLO PRODUCTIVO AGRO-INDUSTRIAL” EN EL CENTRO-SUR DE LA PROVINCIA DE CÓRDOBA, ARGENTINA (1997-2017)
No full textEste trabajo tiene como objetivo comprender los vínculos entre gobiernos locales y actores socio-económicos dominantes para la producción de per-files productivos mediante la herramienta parque industrial. A partir de un estudio de caso, nos centramos en el proceso de construcción y desarrollo del parque industrial municipal de la localidad de General Deheza, Córdoba (1997-2017). Entendemos que constituye un caso paradigmático para dar cuenta del proceso mediante el cual, un proyecto empresarial ingresa a la agenda del gobierno local y deviene en un proyecto socio-político para la ciudad. Se concluye sobre la pertinencia de abordajes empíricos que enfaticen las interrelaciones entre municipio y actores socio-económicos en la producción conjunta de perfiles productivos a nivel ciudad y región
La institucionalización de la evaluación de la política social municipal en México.
No full textLa literatura académica ha dado cuenta de los beneficios que proporciona la evaluación de políticas públicas para la toma de decisiones, sin embargo, estas acciones pueden tener un carácter efímero en caso de que no se encuentren institucionalizadas. Ante este panorama, el contexto de la institucionalización de la evaluación de política social en México ha mostrado grandes avances a partir de la creación del Consejo Nacional de Evaluación de la Política de Desarrollo Social (CONEVAL); no obstante, se tiene poca información sobre la influencia de estos cambios en los gobiernos municipales. Por lo anterior, el objetivo de este trabajo es presentar una aproximación a los principales elementos que permiten incrementar la posibilidad de que se realice evaluación de política social municipal para obtener información que beneficie la toma de decisiones. Para cumplir con dicho objetivo se realiza un análisis exploratorio de la institucionalidad de la evaluación en los programas de Apoyo a Mujeres Jefas de Familia y en Situación Vulnerable y de Apoyo a Jefas de Familia, implementados en los municipios de Zapopan y Tlajomulco de Zúñiga, ambos en el estado de Jalisco, México. Dicho ejercicio se realiza considerando la existencia de demanda y oferta para la evaluación, la determinación de recursos y mecanismos para realizar estos procesos y la definición del uso y difusión de los resultados obtenidos mediante la evaluación de política social municipal
Efectos morfológicos y bioquímicos del ácido carnosico en células HepG2 del carcinoma hepatocelular humano.
No full textAntecedentes / objetivo : La muerte celular autofágica y la apoptosis de células tumorales se ha convertido en uno de los principales objetivos en el tratamiento del cáncer, mientras que las líneas celulares tumorales se utilizan principalmente en estudios para proporcionar datos importantes para la evaluación de posibles sustancias anticancerígenas. En este estudio, nuestro objetivo fue evaluar los cambios morfológicos y bioquímicos, incluida la tasa de apoptosis y los niveles de alfa fetoproteína (AFP) a diferentes concentraciones de ácido carnósico (CA) en células de carcinoma hepatocelular humano HepG2. Materiales y métodos : Carcinoma hepatocelular humano (células HepG2 de séptimo pase). Las líneas celulares se cultivaron en cubreobjetos de vidrio Schott D263M de 11 µM colocados en placas de 12 pocillos y se trataron con DMSO, concentraciones de CA 1, 2,5, 5 y 10 µM durante 24 horas. 48 y 72 horas. Los datos morfológicos y bioquímicos se registraron diariamente, incluidas las tasas de apoptosis demostradas por Caspasa 3, las expresiones de Anexina V bajo luz invertida y microscopía de inmunofluorescencia, luego se analizaron los datos para determinar la significación estadística. Los niveles de AFP, albúmina y ganancias totales se analizaron espectrofotométricamente para evaluación bioquímica. Resultados : Nuestros resultados muestran que CA inhibió significativamente la proliferación de células HepG2 de una manera dependiente de la dosis y el tiempo y causó significativamente la formación de vacuolas autofágicas comenzando desde 5 µM y alcanzando significancia a concentraciones de 10 µM. Se produce una disminución significativa en la AFP cuando se examinan las expresiones de 48 y 72 horas, alcanzando el nivel más bajo a las 72 horas en el grupo de CA 10 µM. Además, el aumento en los niveles de albúmina alcanzó la significación solo en el grupo de 48 h, mientras que también se observaron aumentos no significativos en los grupos de 24 hy 72 h. Conclusión: Nuestro estudio actual demuestra un aumento significativo en las tasas de apoptosis por el ácido carnósico principalmente a concentraciones de 10 µM, lo que respalda su efecto anticancerígeno en las células HepG2. Estos hallazgos también están respaldados por cambios en los análisis bioquímicos de los niveles de albúmina y AFP a concentraciones de 10 µM.
Antecedentes / objetivos: La muerte celular autofágica y la apoptosis de células tumorales se ha convertido en uno de los principales objetivos en el tratamiento del cáncer, mientras que las líneas celulares tumorales se utilizan principalmente en estudios para proporcionar datos importantes para la evaluación de posibles sustancias anticancerígenas. En este estudio, nuestro objetivo fue evaluar los cambios morfológicos y bioquímicos, incluida la tasa de apoptosis y los niveles de alfa fetoproteína (AFP) a diferentes concentraciones de ácido carnósico (CA) en células de carcinoma hepatocelular humano HepG2. Materiales y métodos: Carcinoma hepatocelular humano (HepG2) .Las líneas celulares se cultivaron en cubreobjetos de vidrio Schott D263M de 11 µM colocados en placas de 12 pocillos y se trataron con DMSO, concentraciones de CA 1, 2,5, 5 y 10 µM durante 24, 48 y 72 horas. Los datos morfológicos y bioquímicos se registraron diariamente, incluidas las tasas de apoptosis demostradas por Caspasa 3, las expresiones de Anexina V bajo luz invertida y microscopía de inmunofluorescencia, luego se analizaron los datos para determinar la significación estadística. Los niveles de AFP, albúmina y ganancias totales se analizaron espectrofotométricamente para evaluación bioquímica. Resultados: Nuestros resultados muestran que CA inhibió significativamente la proliferación de células HepG2 de una manera dependiente de la dosis y el tiempo y causó significativamente la formación de vacuolas autofágicas comenzando desde 5 µM y alcanzando significancia a concentraciones de 10 µM. Se produce una disminución significativa en la AFP cuando se examinan las expresiones de 48 y 72 horas, alcanzando el nivel más bajo a las 72 horas en el grupo de CA 10 µM. Además, el aumento en los niveles de albúmina alcanzó la significación solo en el grupo de 48 h, mientras que también se observaron aumentos no significativos en los grupos de 24 hy 72 h. Conclusión: Nuestro estudio demuestra un aumento significativo en las tasas de apoptosis por el ácido carnósico principalmente a concentraciones de 10 µM, lo que respalda su efecto anticancerígeno en las células HepG2. Estos hallazgos también están respaldados por cambios en los análisis bioquímicos de los niveles de albúmina y AFP a concentraciones de 10 µM
Sandwich Osteotomy With Interposition Of A Bovine Block Bone Graft for Vertical Ridge Augmentation
No full textThe aim of the present study was to report the clinical case of a patient with a vertical defect of the alveolar ridge, which prevented the installation of dental implants without first treating the defect in question. A 32-year old female patient with a height defect of approximately 6 mm in the region of the missing absent teeth (13 and 14). The patient was treated using the sandwich osteotomy technique, with the interposition of a block bone graft of bovine origin. No complications were reported in the post- operative period. After seven months, two dental implants were installed in the relevant region. The bovine bone graft was incorporated into the relevant area. The bovine bone block graft used in this clinical case was shown to be a viable option for interposition between bone segments that have been osteotomized via sandwich osteotomy