SCTIMST DSpace (Sree Chitra Tirunal Institute for Medical Sciences and Technology)
Not a member yet
4964 research outputs found
Sort by
Differentiation of Human Adipose Tissue Derived Mesenchymal Stem Cells to Functional Hepatocytes like Cells: Exploring the Niche and Admscs Conditioned Media
No full textTissue engineered approaches have been used for hepatic regeneration and growing hepatic cells in-vitro, however it remains challenging due to the lesser proliferative capacity of the hepatic cells as well as cell source availability due to damaged tissues. Stem cells could be an alternative strategy if differentiated to functional hepatic like cells by providing suitable niche or culture conditions. In the present study we tried to explore differentiation of mesodermal origin cells to the endodermal origin hepatic cells under different culture conditions including fibrin niche and adipocytes derived mesenchymal stem cells conditioned medium. Fibrin provides better cell adhesion and proliferation. Mesenchymal stem cells were isolated and characterized. Two step induction was used for differentiating into hepatic like cells. Functionality of the differentiated hepatic like cells was analyzed using Cyto P 450, glycogen storage and urea/albumin assay at different time points. It was observed the fibrin niche supports the hepatic differentiation and differentiated cells shows hepatic functions. This approach may be used to generate alternative cell source for hepatic tissue engineering
Comparison of oximetry with cardiac magnetic resonance imaging for estimation of flow in bidirectional glenn shunt
No full textFactors associated with adherence to guideline-directed treatment among heart failure patients in Kerala
No full textCombinatorial Application of Hyaluronic Acid and Curcumin-Albumin Conjugate for Cartilage Repair in TNF- α Induced Inflammation in Rabbit Knee Joint
No full textOsteoarthritis has emerged as a consequential disorder resulting from changing lifestyles, especially in the aged population. It is one of the most devastating degenerative joint diseases caused due to inflammation, wear and tear of articular cartilage leading to irreversible damage and physical trauma. Several intra-articular formulations are experimented with for restoring damaged cartilage. Many of them failed because of minimal effectiveness in establishing long-term therapeutic potential. We explored the cartilage regeneration potential of Hyaluronic acid (HA) on combining with dimethoxy curcumin-human serum albumin (DMCHSA) conjugate upon intra-articular administration. HA is known to possess immense lubrication property and is a well-recognized visco-supplement. The DMCHSA has the potential to suppress the action of inflammatory markers. So, a combinatorial approach anticipates an ideal therapeutic strategy to overcome the demerits of existing interventions.
Intra-articular injection of Tumor Necrosis Factor-α (TNF-α), repeatedly at 7-day intervals disrupted the cartilage morphology and produced an inflammatory knee joint model to study the therapeutic potential of DMCHSA-HA combination. Into separate inflamed knee-joint cartilage HA, DMCHSA and DMCHSA-HA were administered periodically to highlight the advantage of mixing the latter with the former.
Histopathology and gene expression analysis assessed the restoration potential of the treatment. We observed remarkable restoration of degenerated cartilage upon treatment with the DMCHSA-HA combination. The columnar arrangement of cells, regulated deposition of ECM components such as glycosaminoglycans (GAGs) & collagen, and synchronized expressions of inflammatory marker molecules suggested restoration of the treated defects.
The treatments with DMCHSA, HA, or HSA alone seemed inferior to DMCHSA-HA combination therapy. The study confirmed that the combination therapy restored the damaged cartilage to normalcy
Role of india hypertension control initiative (ihci) on the structure and functions of health system : a study in thrissur district, kerala
No full textEpicardial pacemaker insertion in patients with congenital heart disease – single centre experience
No full text
Nanotechnology in cardiac stem cell therapy: cell modulation, imaging and gene delivery
No full textThe wide arena of applications opened by nanotechnology is multidimensional. It is already been proven that its prominence can continuously influence human life. The role of stem cells in curing degenerative diseases is another major area of research. Cardiovascular diseases are one of the major causes of death globally. Nanotechnology-assisted stem cell therapy could be used to tackle the challenges faced in the management of cardiovascular diseases. In spite of the positive indications and proven potential of stem cells to differentiate into cardiomyocytes for cardiac repair and regeneration during myocardial infarction, this therapeutic approach still remains in its infancy due to several factors such as non-specificity of injected cells, insignificant survival rate, and low cell retention. Attempts to improve stem cell therapy using nanoparticles have shown some interest among researchers. This review focuses on the major hurdles associated with cardiac stem cell therapy and the role of nanoparticles to overcome the major challenges in this field, including cell modulation, imaging, tracking and gene delivery
