SCTIMST DSpace (Sree Chitra Tirunal Institute for Medical Sciences and Technology)
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Nanotheranostic Probe Built on Methylene Blue Loaded Cucurbituril [8] and Gold Nanorod: Targeted Phototherapy in Combination with SERS Imaging on Breast Cancer Cells
No full textRecent advancements in a nanoarchitecture platform for safe and effective targeted phototherapy in a synergistic fashion is an absolute necessity in localized cancer therapy. Photothermal and photodynamic therapies (PTT and PDT) are considered as the most promising localized therapeutic intervention for cancer management as they have no long-term side effects and are minimally invasive and affordable. Herein, we have demonstrated a tailor-made nanotheranostic probe in which macrocyclic host cucurbituril [8] (CB[8]) is placed as a glue between two gold nanorods (GNRs) within ∼3 nm gaps in linear nanoassemblies with exquisitely sensitive plasmonics that exert combined phototherapy to investigate the therapeutic progression on human breast cancer cells. Photosensitizer methylene blue was positioned on CB[8] to impart the PDT effect, whereas GNR was responsible for PTT on a single laser trigger ensuring the synchronized phototherapy. Furthermore, the nanoconstruct was tagged with targeting anti-Her2 monoclonal antibody (MB-CB[8]@GNR-anti-Her2) for localized PTT and PDT on Her2 positive SKBR3 cells, subsequent cellular recognition by surface-enhanced Raman spectroscopy (SERS) platform, and further assessment of the combined intracellular phototherapy. Hence, the current strategy is definitely marked as a proof-of-concept straightforward approach that implies the perfect nature of the combined phototherapy to achieve an efficient cancer treatment
Asialoglycoprotein receptor targeted optical and magnetic resonance imaging and therapy of liver fbrosis using pullulan stabilized multi-functional iron oxide nanoprobe
No full textEarly diagnosis and therapy of liver fibrosis is of utmost importance, especially considering the increased incidence of alcoholic and non-alcoholic liver syndromes. In this work, a systematic study is reported to develop a dual function and biocompatible nanoprobe for liver specific diagnostic and therapeutic applications. A polysaccharide polymer, pullulan stabilized iron oxide nanoparticle (P-SPIONs) enabled high liver specificity via asialogycoprotein receptor mediation. Longitudinal and transverse magnetic relaxation rates of 2.15 and 146.91 mM−1 s−1 respectively and a size of 12 nm, confirmed the T2 weighted magnetic resonance imaging (MRI) efficacy of P-SPIONs. A current of 400A on 5 mg/ml of P-SPIONs raised the temperature above 50 °C, to facilitate effective hyperthermia. Finally, a NIR dye conjugation facilitated targeted dual imaging in liver fibrosis models, in vivo, with favourable histopathological results and recommends its use in early stage diagnosis using MRI and optical imaging, and subsequent therapy using hyperthermia
From diagnosis to parenthood: event history analysis of infertility treatment trajectories in kerala, india
No full textMetabolic alkalosis in the paediatric cardiac intensive care unit - a prospective observational study
No full textRole of PEGylated CdSe-ZnS quantum dots on structural and functional properties of electrospun polycaprolactone scaffolds for blood vessel tissue engineering
No full textConjugation of quantum dots (Q-dots) into tissue engineering scaffolds is extremely beneficial for designing composite scaffolds imparting multifunctional properties. In the present work, we report the efficacy of incorporating the PEGylated CdSe-ZnS (Q-dots) into electrospun poly(caprolactone)(PCL) scaffolds whose properties were evaluated for small diameter blood vessel applications. Incorporation of Q-dots was found to result in significant reduction in fiber diameter of electrospun scaffolds. Corresponding pore size ranges varied from 12 to 36 µm for composite scaffold compared with 12–60 µm in bare scaffold. Both PCL and composite scaffolds were found to possess better mechanical strength and elasticity than that of native arteries. Contact angle measurements revealed hydrophobic nature of scaffolds which significantly improved upon modification with fibrin. PCL/Q-dot composite scaffolds were found to possess optimum micro-structure for supporting uniform endothelial cell growth after 5 days of culture compared to the pristine PCL scaffold
Fabrication and evaluation of porcine cholecystic extracellular matrix scaffold for cardiac tissue engineering
No full textEffect of cyanide ions (CN-) extracted from cassava (Manihotesculenta Crantz) on Alveolar Epithelial Cells (A549 cells)
No full textCassava (Manihotesculenta Crantz) is one of the most important root crops in tropical countries. It is a major source of cyanogenic glycosides viz. linamarin and lotaustralin, and these on breakdown liberate HCN and ketone. Cassava cyanide extract (CCE) from cassava leaves and tuber rinds were formulated as a biopesticide against certain borer insect pests of horticultural crops. Adenocarcinomic human alveolar basal epithelial cells (A549) were treated with three different concentrations (100, 200, 400 ppm) of CCE. The MTT and NRU assays showed dose-dependent cytotoxicity. The DCFH-DA assay does not show any free radical scavenging activity, whereas the NRR assay showed a reduction in the nitrile radicals with an increase in the concentration of the bioactive compound. A negative correlation was found between the concentration of the bioactive principles and mitochondrial and lysosomal functions. Various cellular assays demonstrated the cellular response of the CCE, and it was found that at higher concentration (400 ppm), the CCE exert a significant necrotic cell death rather than apoptosis. The results of the study indicated that the CCE have a remarkable tendency of anti-proliferative ability
