SCTIMST DSpace (Sree Chitra Tirunal Institute for Medical Sciences and Technology)
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Safety of 0.5% hydrogen peroxide mist used in the disinfection gateway for COVID-19
No full textHydrogen peroxide (H2O2) is a reactive chemical used in a wide range of applications. Most importantly, it is used for sterilization process in health care environment. In the present study, safety assessment of 0.5% of H2O2 and its mist intended to be used in the disinfection gateway for COVID-19 was evaluated. Skin irritation and repeated-dose inhalation toxicity studies were carried out in rabbits and rats, respectively. In Skin irritation study, New Zealand white rabbits were exposed topically with 0.5% H2O2 solution and observed for 24 h, 48 h, and 72 h. For repeated-dose inhalation toxicity study, Wistar rats (both male and female) were exposed (whole body exposure) to 0.5% of H2O2 mist, at a concentration of 11.022 (low dose—2-min exposure), 22.044 (medium dose—4-min exposure), and 55.11mg/kg (high dose/high dose recovery—10-min exposure) body weight, daily for 7 days. Rats in the high-dose recovery group (55.11mg/kg—10-min exposure) were kept for another 7 days without any exposure. A toxicological evaluation was done based on general health parameters, hematology, serum biochemistry, gross necropsy, and histopathological data. The results of the study indicated that there was no skin irritation potential induced on exposure of 0.5% of H2O2 to rabbits. Similarly, the inhalation toxicity of 0.5% of H2O2 mist imparts no evidence of hematological, biochemical, gross pathology, or histopathological abnormalities in rats. Further, at the laboratory condition stimulated, the NOEL was found to be 55.11mg/kg body weight. Hence, the present study concluded that 0.5% H2O2 or its mist used in the disinfection gateway for COVID-19 failed to induce any skin irritation in rabbits or inhalation toxicity in rats
Knowledge and barriers regarding basic life support among civil Police Officers in Trivandrum
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n. In vivo neural tissue engineering using adipose-derived mesenchymal stem cells and fibrin matrix
No full textBackground
The multipotency of adipose-derived mesenchymal stem cells (ADMSC) could be an advantage to regenerate tissues with multiple cell types. However, due to the hostile nature, trauma sites like spinal cord injury can augment the ADMSC differentiation into undesirable lineages. Immersing pre-differentiated neural progenitors in a biomimetic niche during delivery could guard them against any undesired differentiation or death.
Objective
The study proposes using an insoluble cell-specific fibrin niche for in vitro differentiation of rat ADMSCs to neural progenitor cells (NPCs) and oligodendrocyte progenitor cells (OPCs). Further, the study explores fibrin hydrogel for in vivo progenitor cell delivery, and that can aid post-transplant survival/differentiation.
Design
The in vitro experiments analyzed for differentiation-specific markers to establish derivation of rADMSCs to rNPCs and rOPCs. The derived progenitors, tagged with fluorescent tracker dye were delivered in rat T10 contusion SCI using fibrin hydrogel. After 28 days, imaged the experiment site to determine cell survival, immunostained the tissues to identify differentiation of transplanted cells, and evaluated the effect of fibrin and cells on regulating the injury-associated immune response.
Results
The study demonstrated fibrin niche aided stable differentiation of rat ADMSCs into neural progenitors. Fibrin matrix holds up the delivered progenitor cells in the SCI site. The H&E stained tissues revealed regulated cavitation, astrogliosis, and inflammation in test tissues. Progression of transplanted cells into oligodendrocytes upon delivering a mixture of rNPCs, rOPCs, and fibrin is evident.
Conclusion
Fibrin niche-based derivation of neural progenitors from ADMSC seems valuable for transplantation using fibrin hydrogel. It is a promising strategy for extensive study towards further development of translational stem cell-based neural replacement therapy
Impact Of Chemical Angioplasty In patients with Vasospasm and Aneurysmal Subarachnoid Hemorrhage admitted under Neurosurgery department
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Physico chemical characterization of chitosan based scaffold as wound healing biomaterial
No full textSurface Modification of Polypropylene Mesh with a Porcine Cholecystic Extracellular Matrix Hydrogel for Mitigating Host Tissue Reaction
No full textPolypropylene (PP) meshes are widely used for repairing skeletal muscle defects like abdominal hernia despite the chances of undesirable pro-inflammatory tissue reactions that demand revision surgeries in about 45% of cases. Attempts have been made to address the problem by modifying the mesh surface and architecture. These procedures have yielded only incremental improvements in the management of overall postoperative complications, and the search for a clinically viable therapeutic strategy continues. This study deployed a tissue engineering approach for mitigating PP-induced adverse tissue reaction by dip-coating the mesh with a hydrogel formulation of the porcine cholecystic extracellular matrix (CECM). The biomaterial properties of the CECM hydrogel-coated PP (C-PP) meshes were studied and their biocompatibility was evaluated by in vitro and in vivo tests based on ISO standards. Further, the nature of tissue reactions induced by the hydrogel-coated mesh and a commercial PP hernia repair graft was compared in a rat model of partial-thickness abdominal wall defect. Histomorphologically, in comparison with the PP graft-induced tissue reaction, C-PP caused a favorable graft-acceptance response characterized by reduced numbers of pro-inflammatory M1 macrophages and cytotoxic lymphocytes. Remarkably, the differential inflammatory response of the C-PP graft-assisted healing was associated with a fibrotic reaction predominated by deposition of type I collagen rather than type III collagen, as desired during skeletal muscle repair. It was concluded that the CECM hydrogel is a potential biomaterial for surface modification of polymeric biomedical devices
Natural history of coronary stents: long term follow-up of consecutive hundred patients after coronary stenting with drug eluting stents and bare metal stents
No full textAutofluorescence spectroscopy and multivariate analysis for predictingthe induced damages to other organs due to liver fibrosis
No full textWhen our liver does not work well, it can induce damage to other organs causing their dysfunction. With this background, we aim to study the effect of liver fibrosis on other organs such as heart, lungs, kidney and spleen by assessing the variations in the inherent emission property of the tissue, using fluorescence spectroscopy. Fluorescence emission spectra from excised organs of liver fibrosis induced rats were collected at excitation wavelengths 320 and 410 nm. Optical redox ratio derived from the spectral data supported by multivariate statistical analysis, principal component analysis followed by linear discriminant analysis (PCA-LDA) distinguished between control and fibrosis induced groups. The two different excitation wavelength provided variations in the endogenous flurophores collagen, nicotinamide adenine dinucleotide (NADH), flavin adenine dinucleotide (FAD), lipopigments and porphyrins. Additionally, evaluation of redox ratio provided variations in tissue metabolic activity of different organs. The PCA–LDA modelling yielded a sensitivity of 85 to 97% and specificity of 80 to 96% on 320 nm excitation and a sensitivity of 72 to 100% and specificity of 59 to 100% on 410 nm excitation. Fluorescence emission spectral study along with multivariate analysis paved way to identify the biochemical alterations caused to other organs due to the development of liver fibrosis, which could lead to their damage and dysfunction