SCTIMST DSpace (Sree Chitra Tirunal Institute for Medical Sciences and Technology)
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Real-time classification of fnirs data to develop a brain computer interface using deep learning
No full textComparison of transesophageal echocardiography guided modified 2-dimensional and m-mode tricuspid annular plane systolic excursions with transthoracic echocardiography guided m-mode tricuspid annular plane systolic excursion in adult and paediatric cardiac surgeries for assessment of right ventricular function
No full textAnalysis of Whole Blood Prothrombin Time/ International Normalized Ratio Using Image Processing
No full textPurpose: Patients who had undergone mechanical heart valve replacements, who have atrial fibrillation
or deep vein thrombosis, need drugs called oral anticoagulants to prevent blood clotting and need regular
testing of Prothrombin Time/International Normalized Ratio. Conventional laboratory approaches are
time consuming, need blood component separation and a regular visit to clinical labs. The burden of
PT measurement on the clinical laboratory is huge globally, which raise need for point of care, quick
and user-friendly device. Methods: In this study we have proposed a handheld device based on the
image processing for the PT/INR detection. Cost effective disposable strips were fabricated using
thromboplastin as reagent. Device and strips were tested for 100 samples in clinical set up as per the
ISO standard 17593 “Clinical laboratory testing and in vitro medical devices - Requirements for in vitro
monitoring systems for self-testing of oral anticoagulant therapy”. Results: Data was compared with the
values obtained from clinical laboratory using automated coagulometer T Coag DT-100 (Trinity), and
commercially available Point of Care (POC) device from Roche, Diagnostics. A correlation coefficient (r)
of 0.87 & 0.77 was observed between lab vs Chitra device and Chitra device vs commercially available
device, respectively. Conclusion: Clinically accepted correlation may be obtained after automation of
the strip fabrication technique. The proposed device is cost effective and easy to operate and works on
the novel approach of image processing. To best of our knowledge this is the first report on the image
processing-based PT/INR monitoring device
Functional tissue engineered vascular construct as an in vitro model to study the effect of hyperglycemia and hyperlipidemia on endothelial cell dysfunction
No full textJuvenile myoclonic epilepsy: long term outcome and its predictors- a hospital based cohort study
No full textIntegration of Galvanic skin response, skin temperature, Pulse volume changes and Heart Rate variability in Pain monitoring
No full textDevelopment of fibro-porous polymeric membranes for WBC syringe filter by electrospinning
No full textIntracellular inflammatory signalling cascades in human monocytic cells on challenge with phytohemagglutinin and 2,4,6 trinitrophenol
No full textPhytohemagglutinin (PHA) is a plant mitogen that can agglutinate human leukocytes and erythrocytes. PHA is mainly derived from red kidney beans and can act as an exogenous pyrogen. When entering into the blood circulation, exogenous pyrogens principally interact with monocytes and macrophages and induce the release of pro-inflammatory cytokines. Monocytes and macrophages are the cells that fight against foreign invaders and acts as a primary line of immune defence. Similar to PHA, the chemical 2,4,6-trinitrophenol (TNP) also acts as an exogenous pyrogen. The study focused on the in vitro interaction of PHA and TNP with the human monocyte/macrophage cell model THP-1. The exposure and associated change in cellular morphology, organelle function, mechanism of cell death, inflammatory signalling and expression of inflammation-related genes were analyzed in different time periods. It was observed that PHA and TNP induce dose and time-dependent toxicity to monocytes/macrophages where the mechanism of cell death was different for PHA and TNP. Both PHA and TNP can evoke immune signalling with increased expression of inflammatory genes and associated activation of intracellular signalling cascades
An Evaluation Of Variations In The Cerebral Oxygenation and Cerebral Blood Flow Velocity Indices In Various Neurosurgical Positions (Supine, Prone & Lateral) In Elective Neurosurgical Procedures – An Observational Study
No full textGraphene−Gold NanohybridBased Surface-Enhanced Raman Scattering Platform on a Portable Easyto-Use Centrifugal Prototype for Liquid Biopsy Detection of Circulating Breast Cancer Cells
No full textAlthough, cancer is considered as a localized disease in its premature stages, in certain types of cells, it results in metastasis, which accounts for over 90% of the total cancer deaths in the world. During the metastatic stage, cancer cells migrate from the primary tumor site to secondary sites through the circulating bloodstream, resulting in metastasis at unexpected body parts. This situation makes it critical to identify and quantify the circulating tumor cells (CTCs) during the early stages of tumorigenesis. Despite the clinical importance and progress of liquid biopsy and CTC-based cancer diagnostics, it still remains extremely challenging to develop systems to detect CTCs in cancer patients as its presence is extremely low in number (1–10 CTCs per mL of blood plasma). In this article, we discuss about the design and fabrication of a robust and user-friendly custom-designed nanotag-enabled portable filter-based sensor system for the selective separation and isolation of circulating breast cancer cells from whole blood. The filter sensor platform basically consists of a centrifugal prototype to quickly transfer unprocessed blood samples up to 5 mL within 60 s, isolating the circulating breast cancer cells selectively over the anti-EpCAM antibody-immobilized polycarbonate (PC) filter. Moreover, it is also equipped with a sandwich complex of a surface-enhanced Raman scattering (SERS) nanotag comprising a gold–graphene hybrid integrated with anti-ErbB2 antibody (Au-rGO@anti-ErbB2) for the SERS-assisted quantification of CTCs. As compared to the immunoaffinity-based CTC isolation platforms, the centrifugal force-based SERS nanotag enabled filter sensor system proved to be a clog free, highly sensitive, and selective platform toward the rapid isolation of viable breast cancer cells up to 5 tumor cells/mL from whole blood without any sample pretreatment. The high sensitivity, ease of use, and quick availability of results recommends the current filter sensor platform as a universal liquid biopsy technique for the quantification of CTCs