Repositorio de Datos de Investigación de la Universidad de Chile
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132 research outputs found
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La selección de biomarcadores depende de la función genética y del órgano: el caso de los genes de la familia del citocromo P450 en peces de agua dulce expuestos a contaminación crónica
No full textLa contaminación y sus efectos han sido motivo de gran preocupación en las últimas décadas. Se han desarrollado muchas estrategias y marcadores para evaluar sus efectos sobre la biota. Los genes del citocromo P450 (CYP) han recibido mucha atención en este contexto debido a su relación con la desintoxicación y la activación de compuestos exógenos. Si bien su expresión se ha identificado como un biomarcador de exposición a la contaminación, en la mayoría de los casos se ha probado sólo después de exposiciones agudas y para genes CYP asociados con compuestos exógenos. Para dilucidar los patrones de expresión del gen CYP bajo exposición crónica a la contaminación, hemos utilizado como modelo el pejerrey Basilichthys microlepidotus, que habita en la cuenca del río Maipo, un sistema de agua dulce con diferentes niveles de contaminación. Realizamos secuenciación de ARN de próxima generación para tejidos de hígado y branquias de poblaciones contaminadas y no contaminadas. Se observó un patrón de regulación a la baja. La mayoría de los genes CYP asociados con compuestos exógenos mostraron expresión diferencial en las branquias, mientras que los asociados con compuestos endógenos mostraron expresión diferencial en el hígado. Esta respuesta compleja indica que los genes y los tejidos son consideraciones importantes en los estudios que examinan la contaminación utilizando biomarcadores y que su interacción es relevante
Data of Antarctic yeasts transcriptomes
No full textData of expression of ORFs identified in different cellular pathways, including the percentages of amino acids according to their flexibility
Copiapó_Experiment_Catalogue
No full textThe subduction of different features such as oceanic ridges, fractures zones or seamounts are important for the accurate characterization of subduction zones. These structures may affect the along-dip variability of seismic behavior and eventually trigger slow earthquakes. Here we studied the along- dip influence of the Copiapó ridge subduction in the Atacama region, North-Central Chile, an area where seismic swarms, low coupling values and slow earthquakes have been reported. We built a seismic catalog including repeating earthquakes and non-volcanic tremors (NVT), and performed a 3-D tomography for P-wave velocity and Vp/Vs ratio. We identify along-dip segmentation involving 4 distinct segments: (1) a locked seismogenic zone (20 to 35 km depth), hosting ordinary clustered micro-seismicity and repeating earthquakes; (2) a transition zone (35 to 42 km) with non-volcanic tremor and low seismicity; (3) a zone (42 to 60 km) without seismicity and with slow-slip events and (4) a deep zone with more abundant seismicity. The velocity models show differences among these zones: while low velocity anomalies of Vp and Vs, and Vp/Vs ratio between 1.76 and 1.82 are observed in the deep zone, higher velocities are present in the other areas. These features indicate that SSE are located in a zone with higher pore fluid pressure. We propose that these geodynamic differences are generated by the subduction of heterogenic seamounts associated to the Copiapó ridge
Encuesta sobre Ciencia Abierta en la Universidad de Chile
No full textEsta encuesta busca conocer el estado de conocimiento y aplicación de los principios de la “Ciencia Abierta” en la Universidad de Chile, mediante una consulta dirigida a académicos(as), investigador(as) postdoctoral y estudiantes de doctorado. La Universidad de Chile está ejecutando el proyecto “Hacia el cambio cultural en la Universidad de Chile sobre ciencia abierta”, financiado por ANID, el cual tiene como objetivo general “Diseñar y proponer un cambio cultural hacia la Ciencia Abierta, que considere la institucionalidad actual y futura de la Universidad de Chile”. La encuesta no le tomará más de siete minutos y sus respuestas serán de gran utilidad para el proyecto
Replication Data for: "Colorectal cancer trends in Chile: a Latin-American country with marked socioeconomic inequities"
No full textVersion of the dataset used in the paper "Colorectal cancer trends in Chile: a Latin-American country with marked socioeconomic inequities". Public and anonymized data originally available at DEIS: https://deis.minsal.cl/ Some redundant fields (descriptions for ICD-10 codes, geographical names, descriptions of surgeries and procedures) have been removed to reduce file sizes. They can be recovered from the associated codes and their descriptions in the accompanying sheets
Replicar los datos para: Spatial and temporal stability in the population structure of a marine crab despite the long distance between populations, the presence of a biogeographic break, and the main oceanic currents
No full textElucidating the processes responsible for maintaining the population connectivity of marine benthic species mediated by larval dispersal remains a fundamental question in marine ecology and fishery management. Understanding these processes becomes particularly important in areas with a biogeographical break and primarily unidirectional water movement along the sides of the break. Based on single-nucleotide polymorphism (SNP) variability, we determine the population genetic structure, temporal genetic stability, and gene flow among populations of the commercially important crab Metacarcinus edwardsii in a system in southern Chile with a biogeographical break at 42°S. Specimens were collected from eight localities along its geographical distribution, and collection from four of these sites was performed twice. The population genomic approach, assessed with 4,209 SNPs and 234 samples, showed null population differentiation in both the geographical and temporal dimensions. No evidence of effects from the biogeographic break and the northward Humboldt current was detected in gene flow. Migration analyses showed gene flow among all sites but different proportion between pairs of sites. Overall, this research suggests that M. edwardsii comprises a single large population with high gene flow between sites separated by more than 1700 km and demonstrating temporal stability of the population genetic structure
Mitosis en aplastados de células de raíz de cebolla
No full textAplastado de células meristemáticas de raíz de cebolla (Allium cepa) teñidas con orceína acética
Green, yellow or black? Genetic differentiation and adaptation signatures in a highly migratory marine turtle
No full textMarine species may exhibit genetic structure accompanied by phenotypic differentiation related to adaptation despite their high mobility. The genetic differentiation between green turtle (Chelonia mydas) Pacific shape-based morphotypes (south-central/western or yellow turtle and north-central/eastern or black turtle) and the adaptation of the black turtle to environmentally contrasting conditions of the Eastern Pacific region have remained a mystery for decades. Here we addressed both questions using a reduced-representation genome approach (Dartseq; 9,473 neutral SNPs) and identifying candidate outlier loci (67 outlier SNPs) of biological relevance between shape-based morphotypes from eight Pacific foraging grounds (n=158). Our results support genetic divergence between morphotypes, probably arising from strong natal homing behavior. Genes and enriched biological functions linked to thermoregulation, hypoxia, melanism, morphogenesis,osmoregulation, diet and reproduction were found to be outliers for differentiation, providing evidence for adaptation of C. mydas to the eastern Pacific region and suggesting independent evolutionary trajectories of the shape-based morphotypes. Our findings support the evolutionary distinctness of the enigmatic black turtle (north-central/eastern morphotype) and provide a framework for adaptive research and conservation genomics in long-lived and highly mobile vertebrates