Open Research Oklahoma (Oklahoma State Univ.)
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Precision irrigation to improve greenhouse production by reducing water and fertilizer inputs of geranium, petunia, chrysanthemum, and poinsettia
No full textThree different studies investigated optimal irrigation and fertilizer strategies to enhance water and nutrient use efficiency in ornamental crop production. For experiment 1, geranium and petunia plants were subjected to various irrigation methods (manual, tensiometer, and timer-based controller) and fertilizer rates (7 and 10 g controlled-release fertilizer) in a greenhouse. Petunia plants showed no significant interaction or main effects, while the -15 cb tensiometer setting for SPAD values and timer-based controller for root dry weight for geranium was significant. Water use efficiency (WUE) was not significant for either crop. In experiment 2, irrigation and fertilizer effects were again evaluated on poinsettia and chrysanthemum plants. For poinsettia, SPAD reading were greatest with -20 cb tensiometer irrigation and 10 g fertilizer, while all other parameters were greatest for the manual but not different than the timer-based controller. Chrysanthemum height, number of flowers, shoot and root dry weight were greatest with timer-based controller irrigation and 10 g fertilizer. There was an irrigation effect but the -15 and -20 tensiometers demonstrated the greatest WUE for poinsettia. In experiment 3, geranium growth and flowering was evaluated under different timer-based irrigation and fertilizer (3, 6, and 9 g) schedules. Watering 1 minute in the mornings with 6 g of fertilizer led to increased plant height, shoot dry weight, flower production, and WUE. Timer-based controlled irrigation methods showed promise in optimizing water and nutrient utilization, suggesting their role in reducing labor costs while maintaining crop quality. Optimum fertilizer rates varied from 6 or 10 g depending on the species
Improving reading fluency of high school English language learners with 'Readers Theatre' and TikTok videos
No full textReading Fluency is a key skill classified as the bridge to comprehension and an overall factor in student academic achievement. While the official language of Belize is English, the vast majority of Belizean Students are English Language Learners (Handbook of Policies and Procedures, 2000). Therefore, they may be at a disadvantage if they are not reading fluently as they enter First Form or high school. The purpose of this study was to examine the effectiveness of matching the evidence-based reading instruction strategy of ‘Readers' Theatre’ with the use of TikTok videos in improving the oral reading fluency of First Form students. A quasi-experimental pre-post study design was implemented to hypothesize the effect of Traditional Readers’ Theatre and Readers’ Theatre paired with TikTok on reading fluency scores of First Form high school students after a 10-week intervention. The data was analyzed using IBM SPSS Statistical Software version 29. Both descriptive and inferential statistics were computed. The results of the analysis of Covariance (ANCOVA) were used to answer research questions 1 and 2 to observe if there was a significant difference in reading fluency post-test scores between those students who received reading instruction through Traditional Readers’ Theatre and Readers’ Theatre paired with TikTok versus those who did not, controlling for the pre-test scores. Secondly, if there was a significant gender instruction mode interaction among males and females who were engaged in the Traditional Readers Theatre and Readers’ Theatre paired with TikTok intervention versus those who did not, after controlling for pre-test scores. The research study concluded that Readers’ Theatre paired with TikTok reading fluency intervention significantly affected students' reading fluency. Recommendations for policy, practice, and future research are suggested
From the attic above the pond
No full textBecause my mom didn't get her green card or her driver's license until I was in middle school, and because my nuclear family unit was far too religious to tolerate how much the rest of my family enjoyed skipping church, I spent large, unbroken chunks of my childhood at home with my mother, whom I quickly identified as vastly different from every other person I knew. From a very young age, I was encouraged to correct her broken English, a struggle unique to her alone which did not seem to massively improve. My dad and his parents discussed the way that she would talk to her sisters on the phone like there was a punchline fundamentally obscured from my view. I dreamt of her speaking with perfect grammar only once, and it bothered me for many years that I couldn’t explain why I felt too afraid to obey or reply. As a little girl, witnessing my mother navigate through her life taught me that there is power and privilege in being able to fine-tune language so that it snags on the intangible sensation of empathy.
My honors thesis and poetry chapbook From the Attic Above the Pond dwells among themes of gender, language, love, and identity in poems such as "Modesty" "True Mother," and "On Being the Daughter of an Immigrant." These themes affected the relationship I had with my mother, a Filipino mail-order bride, as a mixed person growing up in the southern Bible Belt. Furthermore, my poems discuss information and its accessibility, such as in "Wikipedia" and "It's Like That Documentary, in the North Korean Computer Room" — a theme that ties to identity and language because of how closely fluency in English monitors how much of the world is truly intellectually available to them. Within these specialized vocabulary clusters, my verses dance upon the themes of science, abstraction, divinity and history, which inspire poems like "Seeing Cueva de las Manos," "Conception," and "The Universe-Rug Store."
From the Attic Above the Pond is dedicated to the vibrant and colossal beast that is the invisible labor of women, and to the tether I have always seen around my mother's foot. When she is searching for a word and I find it for her, she peels back my world like a curtain, and each time my poetry is already there
Quantifying the influence of instream sand and gravel mining and inefficient environmental regulations on stream health: A study of Saline Creek
No full textInstream sand and gravel mining (SGM) often leads to significant environmental degradation. As the second most extracted mineral by volume on Earth, sand and gravel are critical for construction materials like cement. Extraction has surged globally in the past decade, particularly in countries such as China and India due to rapidly growing populations. The ecological and socioeconomic impacts of SGM are poorly understood. Previous studies have inferred short-term riverine impacts from SGM but have generally been limited to "snapshot" analyses or have been narrowly focused. The extraction of sand and gravel can cause severe global impacts and potentially irreversible damage to morphological, chemical, and biological stream health. As a result, significant knowledge gaps remain in our understanding of SGM mining impacts. Furthermore, the sand and gravel mining industry is arguably the least regulated extraction industry plagued by illegal mining activities. Such illegal mining often occurs in overlooked rural areas, leading to ineffective management and reporting. This unregulated and unreported extraction complicates the analysis of SGM regulations and their local impacts. Nonetheless, some states have implemented stringent regulations, monitoring, and performance measures. The 2011–2017 court case, David Benham v. Ozark Materials River Rock LLC (Case No. 11-CV-339-GKF-FHM), highlights the impacts of instream SGM. Saline Creek is part of the Ozark Highland ecoregion in Mayes County, northeastern Oklahoma. Mining activities at the lowest reach of Saline Creek between the mid-1990s and 2019 altered the ecosystem, bordering the transition zone into lower Lake Hudson. This dissertation consists of three chapters: one that examines the regulatory performance of SGM in the United States based on industry-related variables, and two that investigates the fluvial-geomorphological and environmental impacts of the small sand and gravel mine on Saline Creek in northeastern Oklahoma
Controlling sheep parasites
No full textThe Oklahoma Cooperative Extension Service periodically issues revisions to its publications. The most current edition is made available. For access to an earlier edition, if available for this title, please contact the Oklahoma State University Library Archives by email at [email protected] or by phone at 405-744-6311
Detection of impairing substances in blood and oral fluid of drivers: Implications for policy and practice
No full textIntroduction: This research aims to synthesize the findings of the Louisiana Oral Fluid Pilot Program and to evaluate the implications of poly-drug use in drug-impaired driving (DUID) cases. It also examines the effectiveness of evidentiary oral fluid (EOF) testing as an alternative to traditional evidence collection methods, the impact of “stop-testing” protocols on DUID data reporting, the technology needed to perform this testing, and overall legal considerations for states considering the adoption of this methodology. Methods: This research occurred in two separate events, first a preliminary training event that aimed to assess the effectiveness of EOF alongside drug recognition expert (DRE) evaluations in identifying impairment of 20 individuals arrested and booked at a county jail. The second event served as the formal pilot program, which spanned over six months in 2023 resulting in the collection and analysis of 103 EOF samples from impaired drivers. The prevalence of poly-drug use, the effectiveness of EOF testing, and the combination of substances used are quantified and the effectiveness of EOF testing as an alternative or complementary specimen to blood is evaluated. The impact of stop-testing protocols on the reporting and detection of relevant data in DUID cases is also assessed. Results: This research revealed a high frequency of poly-drug use (48.5%) in DUID cases. The combination of ethanol and other drugs is common, and the prevalence of ethanol detection raises concerns about impaired driving. The findings support using EOF as a viable alternative to traditional evidence collection methods, with EOF being collected approximately 38 minutes faster on average than blood samples. The research also shows that stop-testing protocols significantly affect the reporting and interpretation of DUID data, leading to the underreporting of drug involvement in DUID cases. Conclusion: This research contributes to the understanding of poly-drug use in DUID cases and the implications for safety and enforcement. It highlights the potential of oral fluid testing as a viable tool in DUID investigations and emphasizes the need to reevaluate stop-testing protocols to ensure comprehensive toxicological analysis. The findings provide valuable insights for oral fluid policy development and future research in the field of DUID
Development of a multiplex assay to characterize ADAMTS13 structure and function
Get PDFIntroduction: The metalloprotease ADAMTS13 is composed of metalloprotease (M), disintegrin-like (D), thrombospondin-1 (T), Cysteine-rich (C), and spacer (S) domains, followed by seven T domains and two CUB domains. Genetic or autoimmune deficiency of ADAMTS13 causes thrombotic thrombocytopenic purpura (TTP), which is characterized by life-threatening microvascular thrombosis. We recently uncovered a cryptic mechanism by which ADAMTS13 Dis and Cys-rich VWF binding sites couple to regulate substrate-induced activation of ADAMTS13.Aim: To develop multisubstrate-based assays to evaluate ADAMTS13 activity and function.Method: The novel multiplex ADAMTS13 assay utilized up to 12 fluorogenic substrates prepared from human and bovine VWF A2 sequences. The substrates are chimeric substrates between Cattle VWF (C-71) and Human VWF (H-71). ADAMTS13 Dis- or Cys-rich binding sites were swapped between H-71 or C-71 peptide backgrounds. Disease-causing mutations were introduced into the wild-type ADAMTS13 by mutagenesis. Wild-type recombinant ADAMTS13 and variant proteins were expressed transiently or stably in HEK293 cells. The animal plasmas used in the current work has been described previously. Assays were performed as we described before, and mean results were reported.Results: Ten novel chimeric FRET substrates were prepared by swapping unique amino residues between cattle VWF71 (C-71) and human VWF71 (H-71). Simultaneous cleavage of all 12 substrates by species-specific ADAMTS13 led to distinctive cleavage rates for each plasma ADAMTS13. This was expected because each species ADAMTS13 has its protein sequence and posttranslational modifications that dictate its interaction with the substrate VWF. Peptide substrates that showed stringent cleavage rates across multiple species were selected for additional validation. These substrates differed mainly between the ADAMTS13 Dis and Cys binding sites. Human pooled normal plasma (PNP), recombinant human ADAMTS13, and ADAMTS13 variants were assayed further by the multiplex ADAMTS13 activity method. Consistent with our expectation, recombinant ADAMTS13 expressed in HEK293 cells showed cleavage rates slightly different from the plasma ADAMTS13. Remarkably, the multiplex assay was able to distinguish truncated ADAMTS13 proteins and recombinant ADAMTS13 with mutations that cause partial activity decreases from the wild type.Conclusions: The ADAMTS13 multiplex assay can be used to evaluate structural perturbations in ADAMTS13. This assay may be useful to test ADAMTS13 function in diseased states where a near-normal ADAMTS13 activity is reported by conventional methods
Sorghums for sirup
No full textThe Oklahoma Cooperative Extension Service periodically issues revisions to its publications. The most current edition is made available. For access to an earlier edition, if available for this title, please contact the Oklahoma State University Library Archives by email at [email protected] or by phone at 405-744-6311
What to expect as a first time online composition instructor and the importance of advising faculty
No full textFirst time online composition instructors face many unique situations in their inaugural term, including challenges such as appropriately implementing technology, enabling effective communication between themselves and students, and ensuring steady access to the internet connection necessary to stay on top of their duties as instructors. However, these new instructors also experience advantages that stem from teaching online, including a heightened level of flexibility in their teaching and communication with students, and the potential for reduced overall costs. In light of the many unique situations that first time online composition instructors face, educational institutions should assign each of their new instructors an advisor who can offer expertise, resources, and guidance to these new instructors to support their endeavors and help them in avoiding burnout
Critical point based experimental design for growth curve estimation
No full textChange and trends in a population can be studied by collecting data at specific time points over a time range. Sampling schedule and sample size allocation are crucial experimental design factors. This study aims to find the best combination of sampling schedule and amplitude for common nonlinear sigmoidal growth models like logistic and Gompertz curves.The proposed design, critical-point based design is based on identifying key growth points; absolute acceleration, maximum acceleration, inflection, maximum deceleration, and asymptotic deceleration points and allocating sample sizes based on predetermined growth phase ratios, with a set minimum number of samples per occasion. Simulation studies are used in validating the methodology under various sampling schemes by assessing the bias, mean squared error, 95\% confidence interval width and coverage of parameter estimates. Also, the fitted growth curves are compared using dynamic time warping (accumulated similarity scores along an optimal path.) The critical point-based design produced estimates with metric values comparable to the 1-unit, 2-unit time jump and locally d-optimal schedules. Additionally, the robustness of logistic model is demonstrated for different variance structures. Furthermore, it is shown that the derived design provides an efficient solution to the optimal design problem and is robust with respect to the misspecification of the unknown distribution and parameters. The application of the sampling design is illustrated through its potential use in explaining the growth process of pecan nuts. In conclusion, the unequal critical point-based design is a good choice in practice, considering the computational advantage relative to the locally d-optimal design and its economical use of experimental resources