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Isolation and Identification of Antiplasmodial Compound from Methanol Extract of Calophyllum bicolor P. F. Steven
No full textCalophyllum bicolor (Clusiacea) is a big tree from Indonesian rain forest in Palangkaraya, Central Kalimantan. Calophyllum or bintagor is one of many sources of natural bioactive compounds that can be used in the fields of health and pharmaceuticals. The aim of this research was to explore the antiplasmodial activity of methanol extract of Calophyllum bicolor P.F. Steven against Plasmodium falciparum. The methanol extract was purified by colomn chromatography system, hexane – ethyl acetate was used as solvent with increasing polarity. One pure compound was obtained and was elucidated based on the 1H-&13C-NMR and 2D-NMR, [COSY, HMBC and HMQC] data and the isolated compound was identified as xanthone. Methanol extract showed antiplasmodial activity growth inhibition against P. falciparum with IC50 5.2 ppm and the new 5-methoxy trapezifolixanthone compound have maximum inhibition at concentration 0.11 nMol. 
Massive In Vitro Propagation of Sandalwood Through Friable Embryogenic Callus
No full textSandalwood (Santalum album), which belongs to Santalaceae family, is a commercially important tree in Indonesia due to its essential oil contents used for important essence of perfume in the perfumery industry. However, its population has significantly depleted since the planting materials of this tree using conventional methods are difficult to be provided. This study was conducted to mass propagate sandalwood using in vitro methods through friable embryogenic callus (FEC). The somatic embryos were formed using leaves in length of 1-3 and 4-7 mm cultured in MS medium containing 0.5 mg/l and 1 mg/l indole acetic acid (IAA), MS medium supplemented with 1 mg/l IAA and 0.2 mg/l kinetin and half concentration of MS medium supplemented with 1 mg/l Gibberellic acid (GA3). Primary somatic embryos (PSE) and secondary somatic embryos (SSE) then formed friable embryogenic callus when it repetitively transferred into MS medium supplemented with 1.7 mg/l BAP, 1 mg/l proline or 1.5 mg/l BAP and 1.2 mg/l kinetin every 3 weeks. The FEC shows its optimum maturation and regeneration in the MS medium supplemented with 1.5 mg/l BAP and 1.2 mg/l kinetin for 4-8 weeks. The acclimatization of sandalwood plantlets were perfectly conducted in the medium containing soil, sand and compos in ratio of 1:1:1 with the companion plant namely Murraya paniculata, (L) Jack which gave the best percentage of survival rate and the lowest percentage of fallen leaves. These findings may improve the massive propagation of sandalwood through FEC as well as a useful material for further genetic improvement of sandalwood by using FEC as material for genetic transformation
Overproduction and Purification of Soluble Recombinant Human Granulocyte Colony Stimulating Factor in Escherichia coli Using Thioredoxin as Fusion
No full textRecombinant human Granulocyte Colony Stimulating Factor (G-CSF) has been produced in a soluble form in Escherichia coli BL21 (DE3) as a fusion protein. The open reading frame of G-CSF was synthetically constructed in previous work and was codon optimized for best expression in E. coli. In this research, the gene was fused to thioredoxin (Trx) at the N-terminal in pET32 vector. The purpose of this research was to optimize the overproduction and purification processes to obtain high yield recombinant protein in soluble form, and to characterize the Trx-G-CSF fusion protein. Overproduction was performed using IPTG induction method for 3 and 6 hours. The protein was purified by Ni-NTA affinity chromatography and separated using gradient concentration of imidazole. The purified protein was then characterized by SDS-PAGE and Western Blot analysis. Further, enterokinase was used to separate G-CSF from the fusion protein. The purified form of G-CSF was subsequently characterized using Western Blot and mass spectrometry using MALDI-TOF. The results showed that the fusion protein was successfully produced in soluble part as much as 48.25% were obtained after 3 hours of induction. The yield of fusion protein was 67.37% from total protein (229.65 mg protein/L culture). The Western Blot analysis showed the G-CSF band at around 18.6 kDa. Mass spectrometry with MALDI-TOF/ TOF revealed that 25.86% of amino acid residue was recognized as part of human G-CSF sequence
Recent Progress on Colorimetric Sensor for Hg(II) Detection
No full textMercury (Hg) contamination presents significant effects on human health and the environmental, particularly caused by its bio-accumulative and neurotoxic properties. Effective detection of Hg(II) is essential for monitoring and mitigating its impact. Among various detection methods, colorimetric sensors have developed into a promising solution due to their affordability, simplicity, and real-time detection capabilities. This review summarizes recent advancements in colorimetric sensors for Hg(II) detection, focusing on innovative materials such as silver nanoparticles (AgNPs), gold nanoparticles (AuNPs), paper-based substrates, agarose biopolymers, and carbon dots (CDs). AuNPs and AgNPs are highlighted for their unique optical properties and sensitivity, enabling visible detection through surface plasmon resonance changes. Paper-based sensors offer portability and low-cost solutions, while agarose serves as a stable biopolymer matrix for immobilizing detection agents. CDs, with their tunable fluorescence properties and green synthesis potential, provide a biocompatible option for hybrid sensing systems. Despite significant progress, challenges such as stability, selectivity in complex matrices, and scalability of green synthesis remain. Future research should focus on integrating these sensors into portable platforms and enhancing their performance for field applications. This review underscores the critical role of innovative materials and sustainable techniques in advancing mercury detection technologie
Pilihan rasional memilih SMK di era Covid-19: Studi kasus SMK Taman Wisata Kota Bogor
No full textThis paper aims to analyze the rational choices of new students at SMK Taman Wisata in choosing a tourism vocational school amid the Covid-19 pandemic and to describe the various goals of new students after graduation, along with the resources they have. This study uses a qualitative approach with a case study method through interviews, field observations, and documentation with the research subject of 5 new students of SMK Taman Wisata from the four skill competencies. Based on the findings, the rational choice of new students in choosing a tourism vocational school in the Covid-19 era is divided into three factors. Sociologically, new students choose tourism vocational schools because they are influenced by success stories from alumni and those closest to them. Economically, choosing the tourism vocational high school is considered less costly than other high school options. Psychologically, there is a desire to realize their dreams and want to work immediately after graduation. The goals of each of the five new students choosing a tourism vocational school are different, including working in accordance with their field and pursuing their goals. However, this goal is not a single goal; there are other goals as an alternative strategy if the main goal is not achieved
Studi Perbandingan Thrust dan Torque pada Toroidal Propeller dengan Wageningen B-Series Propeller Menggunakan Simulasi Computational Fluid Dynamics (CFD)
No full textPropeller berperan penting dalam sistem propulsi kapal, memengaruhi kecepatan dan efisiensi transportasi laut. Wageningen B-Series Propeller menjadi standar dalam analisis hidrodinamis, sementara Toroidal Propeller menawarkan keunggulan dalam torsi serta pengurangan noise dan getaran. Penelitian ini membandingkan karakteristik hidrodinamis dan performa propulsi kedua propeller menggunakan simulasi CFD dengan model turbulensi Transition k-kl-ω. Analisis mencakup Thrust, Torque, KT, dan KQ pada rentang 1500–3000 RPM. Hasilnya, Wageningen B-Series menghasilkan Thrust lebih tinggi, mencapai 2610 N pada 1500 RPM dan 3101 N pada 3000 RPM, dibandingkan Toroidal yang hanya 654 N dan 1591 N. Namun, Toroidal unggul dalam torsi, meningkat dari 67 Nm pada 1500 RPM menjadi 205 Nm pada 3000 RPM, sedangkan B-Series hanya 138,58 Nm. Kesimpulannya, Wageningen B-Series lebih unggul dalam Thrust, sementara Toroidal lebih baik dalam torsi dan potensi pengurangan kebisingan. Optimasi geometri Toroidal Propeller diperlukan untuk meningkatkan daya dorong dan efisiensinya agar lebih kompetitif di industri maritim
Optimization of Average Rice Price Forecasting in East Java Using Decomposition-Weighted Fuzzy Time Series with Lagrange and Differential Evolution
No full textAccurate rice price forecasting is crucial for East Java's economic stability. East Java stands as Indonesia's top rice-producing province, supported by data from Badan Pusat Statistik (BPS) published in October 2024, which highlights an impressive production volume of 9 million tons, outperforming all other provinces in the country. Using the Weighted Fuzzy Time Series (WFTS) model combined with Lagrange Quadratic Programming (LQP) and Differential Evolution (DE), this study forecasts rice prices from January to July 2024 using data from https://panelharga.badanpangan.go.id. The WFTS-LQP-DE model enhances accuracy by refining forecasts through DE, showing improvements over the WFTS-LQP model with lower Mean Squared Error (MSE), Root Mean Squared Error (RMSE), and Mean Absolute Error (MAE). Recommendations include integrating WFTS-LQP-DE forecasts into planning, improving supply chain management, and establishing a price stabilisation fund.
PEMETAAN PROSPEK KUNJUNGAN WISATAWAN ASAL TIONGKOK DI PASAR GLOBAL
No full textThis study was conducted to identify the potential outbound of China’s tourists. This study analyzed two important aspects in mapping the prospect of tourist. The first aspect is the share on outbound tourists that can help to identify potential tourist visits. The second is Gross Domestic Product (GDP) Per Capita, which represents the level of welfare of the population in a region. The study covered 31 provinces in China. By modifying the Boston Consulting Group (BCG) Growth-Share Matrix, we identified eight provinces in China with the most potentials market prospect, namely: Shanghai, Beijing, Guangdong, Jiangsu, Liaoning, Zhejiang, Shandong and Hubei. These provinces are mostly located in eastern part of China. This study recommends that the Indonesia government needs to be more intensive in promoting Indonesia’s tourist destinations in the eight provinces as mentioned.
 
EMPLOYMENT CONDITION, THE EFFECTS OF HUSBAND’S OCCUPATION, AND EDUCATION ON PARTICIPATION OF MARRIED WOMEN IN THE INDONESIAN LABOR MARKET
No full textA previous study has shown that households with both the head and the spouse joining the labor
force tend to exit from poverty. In Indonesia, women that actively participated in labor market were
relatively small, only around 50% in 2017. Meanwhile, most of the women in Indonesia were married in
2017. A husband was one of the factors affecting their wife’s decision to participate in labor market. This
study investigates married women’s employment conditions and the effects of husband’s occupation and
education on their labor market participation. The research sample consists of women aged 15-year-old
and above, married, and living with their husband. We found that the higher the husband’s education,
the lower married women’s probability of participating in the labor market. Husbands with an informal
occupation increased married women’s probability to be in the labor force
POTENSI EKSPOR EKONOMI KREATIF TAHUN 2019
No full textCreative economy is a potential sector that can be a source of renewable economic growth as a solution for
the government to prevent the slowing down economy. Export from the creative economy indicates a good result.
Export is also one of the components to count Gross Domestic Product (GDP). That means the rise in export from
the creative industry will increase the economic growth from the creative economy, which may lead to the increasing
of economic growth as a whole. The objective of this paper is to know the impact of the creative industry to GDP
growth from the creative industry. In order to get creative economy export in 2019, we forecast creative economy
export using the ARIMA model. The result showed that ARIMA (0,1,1)(2,0,1)12 is the best model to get creative
economy export 2019, which is 20.87 million USD or there is an increase in creative economy exports of 0.70
billion USD from 2018. The additional value of creative economy exports is equivalent to 15.54 trillion rupiahs.
Using Input-Output analysis table of creative economy, it is obtained that the additional creative economy exports
of 15.54 trillion rupiahs will provide an additional creative economy GDP of 7.81 trillion rupiahs. Hence, the
creative economic growth in 2019 will be 0.71 percent. Unfortunately, this creative economic growth is smaller
0.38 percent from 2018