NIV-NS Repository (Naučni institut za veterinarstvo "Novi Sad")
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Experiences in the control of infectious laryngotracheitis on poultry farms in Vojvodina
Chemical treatment strategies for mycotoxin removal: efficiency, mechanisms, and applications
This research was funded by Ministry of Science, Technological Development
and Innovation of Republic of Serbia by the Contract of implementation and funding of research
work of NIV-NS in 2025, Contract No: 451-03-136/2025-03/200031
Seroepidemiological Survey of Hepatitis E Virus in Intensive Pig Farming in Vojvodina Province, Serbia
Hepatitis E virus (HEV) is the causative agent of acute hepatitis E infection
in humans. Two epidemiological patterns of the disease exist—endemic and sporadic.
Genotypes 1 (HEV-1) and 2 (HEV-2) are transmitted through contaminated water and are
responsible for the outbreaks of many large-scale epidemics in developing countries of Asia
and Africa. Genotypes 3 (HEV-3) and 4 (HEV-4) have zoonotic potential and cause sporadic
cases and small outbreaks in high-income countries. The first case of HEV infection in swine
was confirmed in 1997 and later detected in other animal species. The aim of this study
was to investigate the seroprevalence of HEV infection in pig farms in Vojvodina province,
Serbia. Three hundred blood samples were collected from five different categories of pigs
from 3 different farms on the territory of the South Baˇcka district in Vojvodina (Serbia).
The analyses were conducted by in-house ELISA, while the western blot method was
used as a confirmatory test for doubtful results. The presence of HEV IgG was detected
on all three examined farms. The established seroprevalence in Farm A was 37%, 31%
in Farm B, and 54% in Farm C. The mean seroprevalence for all farms was 40.66%. A
higher seroprevalence was found in fatteners compared to younger categories of pigs. We
concluded that HEV is widespread on pig farms with intensive management. Further
analyses should be conducted with the aim of implementing a surveillance program to
prevent possible human infection.This research was funded by the Ministry of Science, Technological Development and Innovation of Republic of Serbia by the Contract of implementation and funding of research work of NIV-NS in 2024, Contract No: 451-03-66/2024-03/200031, the World Bank and EU by the project: Serbia Accelerating Innovation and Entrepreneurship Project—SAIGE, 2022
Metagenomic analysis of viruses in rats reveals diversity and potential zoonotic risks
This project has received resources from the Infravec consortium as part of the ISIDORe project (funding from the European Union’s Horizon Europe Research & Innovation program, grant agreement N° 101046133). The study was supported by the Ministry of Science, Technological Development and Innovation of the Republic of Serbia (Contract numbers 451-03-136/2025-03/20031 and 451-03-136/2025-03/200143)
Presence of USUTU virus in mosquitoes in the Vojvodina Province of Serbia in 2024
The study was supported by the Ministry of Science, Technological Development and Innovation of the Republic of Serbia (Contract numbers 451-03-136/2025-03/20031 and 451-03-137/2025-03/200117)
Anthelmintic activity of winter savory (Satureja montana L.) essential oil against gastrointestinal nematodes of sheep
Background
The increasing difficulties in combating anthelmintic resistance in gastrointestinal nematodes (GINs) of sheep worldwide, and the residues of chemical drugs in animal products and the environment, necessitate the search for alternatives. Previous studies have shown that plant essential oils (EOs) could be valuable anthelmintic agents, due to their numerous advantages. The present study aimed to evaluate the possibility of using winter savory (Satureja montana L.) EO against sheep GINs. The chemical composition of the tested oil was determined by gas chromatography-mass spectrometry (GC–MS). The efficacy of the tested oil was determined in vitro using the egg hatch test (EHT), and in vivo using the faecal egg count reduction test (FECRT) performed in two farms. Preliminary toxicity studies including clinical observation, haematological and biochemical blood analysis were also performed to evaluate the safety of the tested oil to the hosts. In addition, a coproculture study was carried out in the tested farms using the appropriate morphological keys.
Results
Main compounds of the S. montana oil identified by GC–MS analysis were p-cymene (42.8%), carvacrol (28.1%) and y-terpinene (14.6%). The in vitro EHT showed a dose-dependent (R2 = 0.94) anthelmintic potential of the tested oil, with ovicidal activity varying from 17.0–83.3% and determined IC50 value of 0.59 mg/ml. The field efficacy reached 33% (at group level) and 50% (at individual level) at D14 after treatment. In vivo efficacy was significantly higher in farm 2 (FEC above 65% at group level, p 0.05) in the percentage representation of GIN genera were observed in the coproculture study, indicating that the treatment agent was not specific to a single genus.
Conclusion
The anthelmintic potential showed on EHT and FECRT, without adverse effects on the sheep, suggests that S. montana EO is suitable for the control of sheep GINs as part of an integrated parasite management. However, further studies should be conducted to increase efficacy in field conditions.This research was co-funded by the Ministry of Education, Science, and Technological Development of the Republic of Serbia, rescript no. 451–03-1183/2021–14. The research was also co-funded by the Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Italy, as part of the „CREMOPAR “ project
Eradication of Brucella оvis infection from sheep flock
This work was funded by Ministry of Science, Technological Development and Innovation of Republic of Serbia by the Contract of implementation and funding of research work of NIV-NS in 2025, Contract No: 451-03-136/2025-03/200031