NIV-NS Repository (Naučni institut za veterinarstvo "Novi Sad")
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Epidemiological characteristics of brucellosis in Vojvodina, Serbia, 2000–2014
No full textBackground/Aim. Despite the fact that brucellosis occurs
sporadically in the epidemic form, this disease is still one of
the world's most widespread zoonoses. Methods. Data
from the register of infectious diseases of the Institute of
Public Health of Vojvodina and Scientific Veterinary Institute
in Novi Sad were used in this study. Using descriptive
statistics, data were analyzed for the period 2000 to 2014.
Results. In the observed period in Vojvodina 102 cases of
brucellosis were registered with different frequency of notification
cases by districts of province. Most frequent modes
of transmission of brucellosis were consumption of contaminated
food (especially sheep cheese) or direct contact
with domestic animals. In 70.2% of the patients, occupational
exposures to the agent or direct daily contact with animals
were noted. The dominant source of infection in the
urban area was food, whereas a direct contact with sick animals
was dominant mode of transmission in the rural area.
Overall, 14 epidemic outbreaks of brucella were registered
with direct contact as dominant mode of transmission. The
predominant age-range of patients with brucella confirmed
infections was 30–59 years (50.0 male %), and 2.5 times
more males than females were affected. Seasonal distribution
was highest during spring, with 50.0% of all confirmed
cases. During the period 2004–2005, prevalence of serum positive
patients in the South Bačka District coincided with the
highest number of laboratory confirmed serum samples among
animals. Conclusion. Although the incidence of brucellosis
shows a declining trend, education and improving of surveillance
of disease of all relevant institutions seems as necessary
for better recognition and notification of the disease
Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance
No full textIntroduction: The aim of this study was to determine the prevalence of Salmonella along the slaughter line and to identify possible critical
control points in one slaughterhouse facility located in the city of Belgrade.
Methodology: In total, 700 samples were tested: two swabs from both sides of carcass were taken from each of 100 pigs. In this way, 200 pig
skin swab samples were taken after stunning, 200 after processing and 200 after chilling. Additional 100 samples of ileal contents were also
taken from the same pigs to obtain a collection of 270 isolates. All samples were analyzed using standard culture methods and serotyping.
PFGE was performed for 27 isolates. Determination of antimicrobial resistance was performed by E-test.
Results: In total, 47 (23.5%) swab samples were positive for the presence of Salmonella after stunning. After processing, Salmonella was
isolated in two swab samples (1%), whereas all samples which were collected after chilling were negative for Salmonella. The sampling of
ileal contents was positive for five Salmonella isolates (5%). The most frequently isolated serotypes were S. Derby (90.74%), S. Infantis (5.56%)
and S. Typhimurium (3.7%). All tested isolates were resistant to tetracycline. Resistance was recorded to nalidixic acid (23.3%), ciprofloxacin
(20%), ampicillin (10%) and chloramphenicol (14.4%), as well. The PFGE results indicated that isolates had a high genetic similarity.
Conclusions: The investigation has confirmed that bacteriological examinations of carcass swabs, as well as ileal content, could be used to
assess the carriage of salmonellae in pigs at the time of slaughter.This work was supported by the Project III46009 from the
Ministry of Education, Science and Technological
Development of the Republic of Serbia
Virological Quality of Irrigation Water in Leafy Green Vegetables and Berry Fruits Production Chains
No full textThis study condenses data acquired during
investigations of the virological quality of irrigation water
used in production of fresh produce. One hundred and eight
samples of irrigation water were collected from five berry
fruit farms in Finland (1), the Czech Republic (1), Serbia
(2), and Poland (1), and sixty-one samples were collected
from three leafy green vegetable farms in Poland, Serbia,
and Greece. Samples were analyzed for index viruses of
human or animal fecal contamination (human and porcine
adenoviruses, and bovine polyoma viruses), and human
pathogenic viruses (hepatitis A virus, hepatitis E virus, and
noroviruses GI/GII). Both index and pathogenic viruses
were found in irrigation water samples from the leafy green
vegetables production chain. The data on the presence of index viruses indicated that the highest percentage of fecal
contamination was of human origin (28.1 %, 18/64), followed
by that of porcine (15.4 %, 6/39) and bovine (5.1 %,
2/39) origins. Hepatitis E virus (5 %, 1/20) and noroviruses
GII (14.3 %, 4/28) were also detected. Samples from berry
fruit production were also positive for both index and
pathogenic viruses. The highest percentage of fecal contamination
was of human origin (8.3 %, 9/108), followed
by that of porcine, 4.5 % (4/89) and bovine, 1.1 % (1/89)
origins. Norovirus GII (3.6 %, 2/56) was also detected.
These data demonstrate that irrigation water used in primary
production is an important vehicle of viral contamination
for fresh produce, and thus is a critical control point
which should be integrated into food safety management
systems for viruses. The recommendations of Codex Alimentarius,
as well as regulations on the use of water of appropriate quality for irrigation purposes, should be
followed.This work was supported by a European Community’s
Seventh Framework Programme (FP7/2007–2013) under
Grant Agreement No. KBBE-213178 (Integrated monitoring and
control of foodborne viruses in European food supply chains, VITAL)
led by the coordination team of Nigel Cook (FERA, UK), Martin
D’Agostino (FERA, UK), and Franco M Ruggeri (ISS, Italy). The
authors are grateful to the owners of the companies who took part in
this study for their kind and skillful collaboration and assistance, and
to all VITAL members. As it concerns VRI, the results of the project
LO1218 were obtained with financial support from the MEYS of the
CR under the NPU I program
Organochlorine pesticides in canned tuna and sardines on the Serbian market
No full textThe aim of this study was to determine the level of organochlorine (OC) pesticides in 57 samples
of canned tuna and 31 samples of canned sardines in vegetable oil, collected from supermarkets
in Serbia. OC pesticides α-HCH, β-HCH, δ-HCH, dichlorodiphenyltrichloroethane (DDT), DDE, DDD,
dielderin, endosulfane I, endosulfane II, endosulan sulfate, endrin, endrin ketone, heptachlor,
heptachlor epoxide, lindane, aldrin, metoxichlor, cis-chlordane and trans-chlordane were determined
using a GS-MS method. The highest concentrations (μg kg−1, arithmetic means) in canned
tuna were for δ-HCH (60.6 ± 97.0) and p, p´-DDT (55.0 ± 25.1), while the corresponding values in
canned sardines were for δ-HCH (90.7 ± 102.7) and endosulfane II (78.0 ± 145.9). Mean level for
the sum of endosulfans was above the maximum limit in canned sardines (85.0 μg kg−1). Also,
dieldrin (39.7 μg kg−1) was measured above the ML.This study was supported by the Ministry of Education,
Science and Technological Development, Republic of Serbia:
[Grant Number TR 31011/2011-2016]
Fish Product–Borne Histamine Intoxication Outbreak and Survey of Imported Fish and Fish Products in Serbia
No full textHistamine levels in fish and fish products are regarded as a primary criterion for food safety, considering the effects of histamine on human health. The aim of this research was to describe a case of massive histamine intoxication in Serbian children and to provide relevant information regarding the presence of histamine in imported marine fish and fish products available in the Serbian market. In January 2014, an incident of foodborne illness occurred in 28 children (aged 2 to 5 years) who consumed canned sardines in a kindergarten in Vojvodina province, northern Serbia. The diagnosis was established based on anamnestic data, epidemiological data, and clinical symptoms and confirmed by positive histamine finding in the incriminated sardines. Substantially high histamine levels (.300 mg/kg) were detected in seven of the nine examined units of these canned sardines. In addition, during an official veterinary border control from January 2013 to January 2015, 273 lots in total, including 2,457 fish and fishery product units, were analyzed using enzyme-linked immunosorbent assay. Even though only nine (3.29%) of all examined lots were declared as unsafe for human consumption, the presented case of histamine intoxication strongly suggests the importance of border inspection and comprehensive control of each imported lot.This work was supported by grant TR 31011 from the Ministry of Science and Technological Development of the Republic of Serbia, ‘‘The influence of the components and feed quality from the cyprinid fish species on the quality of meat, losses, and the profitability of production
Prisustvo rezidua antibiotika u uzorcima meda od aprila 2013. do aprila 2014. na teritoriji AP Vojvodine, Srbija
No full textThe illegal use of antibiotics in honey production has recently been focused and the presence of antibiotic residues in honey is a critical parameter for its quality as it affects the safety of honey for human population. The need for simple test methods for determination of the presence of antibiotic residues in honey certainly exists. A modified method 4 plates that was primarily developed for detection of antibiotic residues in food was qualitatively applied for the screening of the residues of antibiotics in honey. The test is rapid, simple and cheap. In the present study, the investigations of the presence of antibiotic residues in honey result in 2.59% positive findings, i.e. in 5 of 193 examined honey samples collected from the territory of Vojvodina. Rapid analysis of the presence of antibiotic residues in honey certainly plays an important role in determining the overall safety of honey and final assessment of its usability
The presence of Lindane in different types of honey in the Pannonian region
No full textSixty samples of honey were collected in the Pannonian region of Serbia during the year 2013. Analytical procedure of sample preparation based on the Quechers method was used for determination of pesticide residue content, whereas the quantification of pesticide residue was performed on gas chromatography mass detector. The presence of organochloric pesticides was determined during the research. Lindane was the most frequent pesticide in all types of honey. 73perc. of lindane was found in sunflower honey of the total quantity of detected pesticides, 73 perc. in meadow honey, 85 perc. in linden honey, 58 perc. in forest honey and 97 perc. in acacia honey. The highest lindane concentrations were found in acacia honey in the quantity of 4.45 pg/kg. The content of other pesticide residues is insignificant. Due to the fact that honey is the indicator of environmental pollution and according to the study performed, it can be concluded that higher lindane concentration is present in the Pannonian region honey in comparison with the other organochloric pesticides. Although it was detected, lindane concentration in the Pannonian region honey does not exceed the maximum quantity allowed prescribed in the Rulebook of the Republic of Serbia.his work is supported by a grant from the Ministry of Education, Science and Technological Development, Republic of Serbia, Project number TR 31084 and IPA PANONIAN BEE-increasing of competences of beekeepers in the Cross-border area no 2012/307-81
SADRŽAJ VODE I KISELOST KAO POKAZATELJI KVALITETA MEDA SA PODRUČJA VOJVODINE
No full textThis work is supported by a grant from the Ministry of Education, Science and Technological Development, Republic of Serbia, Project number TR31084 and IPA Project „PANONIAN BEE“ –Increasing of competitiveness in the Cross-border Area No 2012/307-813
