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Diseño y evaluación de un sistema híbrido solar-eólico con almacenamiento mediante baterías y capacitores
No full textLineamientos para la preparacion de licitaciones de obra publica de las MIPYMES con base en los procesos del PMBOk
No full textAntioxidant and chelating activities from lion fish (Pterois volitans L.) muscle protein hydrolysates produced by in vitro digestion using pepsin and pancreatin.
No full textThe aim of the present research was to determine the antioxidant, and Cu2+ and Fe2+ chelating activities, amino acid content and estimated molecular weight of proteins and peptides from lion fish muscle-derived hydrolysates obtained by enzymatic hydrolysis with pepsin and pancreatin. The fillets were freeze-dried and hydrolysis was performed, taking aliquots at different times. The degree of hydrolysis, antioxidant activity against ABTS+ and DPPH, Cu2+ and Fe2+ chelating activity, as well as molecular weight estimated by SDS-PAGE and amino acid content by HPLC was determined. The highest degree of hydrolysis (DH) was at 180 min (37.75%). SDS-PAGE showed proteins with an estimated molecular weight of 45,259 and 42,487 Da, which could be associated with myofibrillar proteins. The progressive degradation of proteins by enzymes was also observed, detecting polypeptides with a EMW of 5,988 and 4,954 Da in the most representative hydrolysates. The H40 hydrolysate exhibited the highest ABTS and DPPH radical depuration activity, with values of 107.31 mM/mg protein and 54.27%, respectively. The iron and copper chelating activity was related to DH, since the highest values of iron and copper chelating activity were obtained in hydrolysates H120 and H140, with 90.83% chelation of Cu2+ and 56.33% chelation of Fe2+, respectively with no significant differences compared to subsequent times. In addition, the antioxidant and chelating activities were possibly related to Trp, Cys, Lys, Pro and Arg content. Lion fish muscle hydrolysates could be a potential source of functional ingredients due to their in vitro antioxidant and chelating activity
Toxicological screening of four bioactive citroflavonoids: in vitro, in vivo, and in silico approaches
No full textMany studies describe different pharmacological effects of flavonoids on experimental animals and humans. Nevertheless, few ones are confirming the safety of these compounds for therapeutic purposes. This study aimed to investigate the preclinical safety of naringenin, naringin, hesperidin, and quercetin by in vivo, in vitro, and in silico approaches. For this, an MTT-based cytotoxicity assay in VERO and MDCK cell lines was performed. In addition, acute toxicity was evaluated on Wistar rats by OECD Guidelines for the Testing of Chemicals (Test No. 423: Acute Oral Toxicity-Class Method). Furthermore, we used the ACD/Tox Suite to predict toxicological parameters such as hERG channel blockade, CYP450 inhibition, and acute toxicity in animals. The results showed that quercetin was slightly more cytotoxic on cell lines (IC50 of 219.44 ± 7.22 mM and 465.41 ± 7.44 mM, respectively) than the other citroflavonoids. All flavonoids exhibited an LD50 value > 2000 mg/kg, which classifies them as low-risk substances as OECD guidelines established. Similarly, predicted LD50 was LD50 > 300 to 2000 mg/kg for all flavonoids as acute toxicity assay estimated. Data suggests that all these flavonoids did not show significant toxicological effects, and they were classified as low-risk, useful substances for drug development
Peer Support Added to Diabetes Education Improves Metabolic Control and Quality of Life in Mayan Adults Living With Type 2 Diabetes: A Randomized Controlled Trial
No full textConsumo frecuente de alimentos industrializados y su percepción en adolescentes indígenas Mayas con sobrepeso y obesidad
No full textSe planteó como objetivo identificar cuáles y de qué tipo son los alimentos industrializados modernos que los adolescentes con sobrepeso y obesidad consumen y la percepción que tienen de ellos. El estudio fue de tipo cuali-cuantitativo, descriptivo e interpretativo; se llevó a cabo con estudiantes adolescentes de cuatro secundarias del municipio de Abalá, Yucatán, México. Constó de tres etapas, en la primera se realizaron mediciones antropométricas a 292 estudiantes de las secundarias para obtener el Índice de Masa Corporal, en la segunda se seleccionaron al azar 58 estudiantes que presentaron sobrepeso y obesidad; a los cuales se les aplicó un cuestionario de frecuencia de consumo de alimentos. En la tercera etapa se realizaron cuatro grupos focales para conocer la percepción respecto al consumo de alimentos. Se observó una ingesta alta de alimentos industrializados modernos en el desayuno, receso escolar y cena; en el tiempo de la comida aún se consumen preparaciones tradicionales. Existe una transculturación de la alimentación en los adolescentes
