Malaysian Journal of Medical and Biological Research
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Antimicrobial Resistance and Sensitivity among Isolates of Esherichia coli from Urine Samples in Denizli, Turkey
Full text linkObjective: The research was carried out with isolate and determines the antimicrobial sensitivity in E. coli from urinary tract infections in special hospital in Denizli and recorded at specimens.
Methods: Urine samples (n=21) were collected from patients with signs and symptoms of Urinary tract infections. Bacteria were isolated and identified by conventional biochemical profile. Antibiotic resistance pattern of E. coli against different antibiotic was determined by Kirby-Baur method.
Results: The results revealed that sensitivity rate of antimicrobial agents were in the range of meropenem (100%), norfloxacin and ciprofloxacin (86%), cefotaxime (80%), aztreonam (76%). None of the samples showed no resistance to amikacin, ceftazidime, aztreonam, amoxicillin/clavulanic acid, and meropenem. Out of 21 isolates, 3(14%) isolates showed Multiple Antibiotic Resistance ten to thirteen antibiotics.
Conclusion: It is concluded that most of the urinary tract infections in human are caused by E.coli exhibited highest resistance to meropenem (100%), followed by norfloxacin and ciprofloxacin (86%)
Regenerative Effects of Wharton’s Jelly Stem Cells-Conditioned Medium in UVA-Irradiated Human Dermal Fibroblasts
Full text linkBackground: Ultraviolet A radiation (UVA) can photo-age skin by suppressing the proliferation, migration, and collagen deposition of human dermal fibroblasts (HDFs). This process occurs because UVA light can inhibit the gene expression of the TGF-β receptor in HDFs. Moreover, Wharton’s Jelly Stem Cells-Conditioned Medium (WJSC-CM) is hypothesized to release microvesicles that contain short m-RNA with regenerative properties.
Objectives: This study aimed to determine the regenerative properties of WJSC-CM on UVA-Irradiated Human Dermal Fibroblasts (UVA-HDFs)
Methods: Passaged fourth of HDFs obtained from the foreskin of six (11- to 13-year-old) boys were repeatedly irradiated with a total of 10 J/cm2 UVA and treated with various concentrations of WJSC-CM. We used non-irradiated HDFs as positive control. After that, the consumption of TGF-β, cellular proliferation, cellular migration, and collagen deposition of each group were measured and compared.
Results: Compared to the non-irradiated groups, the proliferation rates, migration rates, and collagen deposition of UVA-HDFs significantly decreased (p<0.05). WJSC-CM can improve the consumption of TGF-β, proliferation, and cellular migration of UVA-HDFs. However, WJSC-CM failed to improve the collagen deposition of UVA-HDFs (p>0.05).
Conclusions: WJSC-CM has regenerative properties and is a candidate material for the treatment of prematurely ageing skin induced by UVA-irradiation.
Ethical permission: This experiment was permitted by the local ethical permission committee Ref:KE/FK/382/EC with permission letter dated 17-04-2013
Effect of Different Sowing Dates and Varieties on Growth and Yield of Lentil (Lens Culinaris Medikus) in the Highland Vertisols of North Shewa, Ethiopia
Full text linkField experiments were carried out to study the response of two lentil varieties to varying sowing dates in a split plot design with three replications, in which varieties were assigned to main plots and sowing dates to sub plots. The study was conducted at Enewari research site of Debre Birhan agricultural research center for three consecutive years (2007 - 2009) on two soil types. The results showed that no significant difference between varieties for grain yield. However, variety Alemaya produced highest grain yields of 1.3 t/ha and 1.22 t/ha from fifth (30-July) sowing date on heavy and relatively light Vertisols respectively. On the other hand, the local variety produced highest grain yields of 1.4 t/ha and 1.06 t/ha on the fifth and six sowing dates on heavy and relatively light Vertisols respectively. Grain yield proportionally increased with increasing biological yield in different sowing dates on both soil types. On heavy Vertisol varieties responded differently to the changes of sowing dates. Variety Alemaya had responded to a wider sowing dates. Early August to mid-August sowing found to be optimum for local variety. On light Vertisol, the functional relationship was unexplained for both varieties. In general, heavy Vertisol gave higher responses than relatively light vertisol throughout most parameters and levels tested.
 
Artificial Intelligence and IoT in Dairy Farm
Full text linkInternet of things (IoT) and data-driven techniques are producing greater prospects for smart dairying. The demand for milk is unceasingly increasing because of the rising population of the globe. The employment of dairy products is more in developed countries as compared to developing countries. To fulfill this increased demand for milk products, better technological techniques for improving milk yield are required. It’s foreseeable that the use of IoT and different AI techniques can lend a hand to a farmer to beat different conventional farming challenges and increase milk production. During this research, the authors give a talk about different challenges that a dairy farmer has to countenance in their way of life. A brief introduction of smart dairying (SDF) is presented with relevancy to the modernization in production and therefore the processes of smart dairy farming. This review concentrates on different facets of smart dairying, and at last, a state-of-the-art framework that can aid the farmers to extend the milk yield by using different up-to-the-minute technologies has been proposed. These high-tech methods can reduce the factors negatively upsetting milk production and increase that positively heartrending production with trifling resources.
 
The Addition of Hyaluronic Acid into Platelet-Rich Fibrin Lysate in Restoration of Senescent Human Dermal Fibroblasts Activities
Full text linkSenescent human dermal fibroblasts had reduced capacity in proliferation and collagen synthesis. It is due to unresponsiveness against transforming growth factor-β1 (TGF-β1) stimulation. Either platelet-rich fibrin (PRF)-lysate or hyaluronic acid (HA) can restore TGF-β1 signaling pathway.
To determine whether HA addition to PRF lysate has a better activity than PRF-lysate alone in restoring senescent human dermal fibroblasts (HDFs) activities.
HDF isolated from six different human skins was divided into normal HDFs and senescent HDFs which are induced by serum starvation. The senescent groups were then given 50% PRF-lysate and various levels of HA. Amelioration of TGF-β1 signaling was measured by cellular proliferation index and collagen deposition.
Addition of HA into PRF-lysate resulted in a significant increase in proliferation index and collagen deposition index than PRF-lysate alone. The best level of HA for this mixture ranged from 20.83 mM to 41.67 mM. HA in PRF lysate is an excellent candidate material for treating clinical signs related to senescent human dermal fibroblasts.
Ethical permission: This experiment had gain approval from the local ethical committee, Ref: KE/FK/471/EC/2016 dated 17-05-2016
AI Health Check Monitoring and Managing Content Up and Data in CMS World
Full text linkThe advent of artificial intelligence (AI) as a means for improved health care bids extraordinary prospects to advance clinical lineup results and patient, reduce costs, and influence populace health. A1 health check monitoring jobs can take care of executing certain basic and advice rules of cleaning up repositories of data which are not getting used to managing the assets which are referred for a long time deleted that big companies with huge contents and assets struggle in keeping the server up and running 24/7. Thus, the objective of this article is to understand the “why to” and the “how-to” of employing all the major health systems in the CMS world. Also review artificial intelligence when compared to human intelligence in the health sector, Data bias, diversity in artificial intelligence teams, and impacts of artificial intelligence on the patient-provider relationship. To give this subject matter, we deployed literature approaches to examine major content that will help in achieving the purpose of this study. The review shows the need for a combination of artificial intelligence and human intelligence produces an augmented intelligence that focuses on creating a more assisting and supportive role for the algorithm. Also, it portrays trust, equity and inclusion need to be prioritized in the healthcare artificial intelligence development and deployment processes, and data management.
 
Comparative Study of Absorbed Doses in Different Phantom Materials and Fabrication of a Suitable Phantom
Full text linkIn Cancer treatment (radiotherapy) centers, Phantom is important in Quality Assurance routine check and absolute dosimetry conformation. Water is IAEA standard phantom material. But it has some technical difficulty in practical uses and other solid phantoms such as Polystyrene, PMMA is very expensive and locally not available. Hence, the purpose of this paper is to find out a phantom that will be technically very sound, cost effective and locally available. This study reveals that paraffin wax, which has some approximately similar properties (i.e. chemical composition, mass density and number of electrons/gram) to water, can be used as alternative of solid water phantom because of their proximity to the dose absorption property of water which is even better than some of the conventional solid phantoms used in radiation dosimetry. It is also found that paraffin wax phantom with air-bubble inside behaves differently to the radiation absorbing dose and therefore in dose absorption and dose conversion (scaling) factor.
 
HPTLC Chromatographic Polyphenolic Fingerprints of Plant Species from Eastern Europe
Full text linkPolyphenols are plant secondary metabolites. They are important active principles from medicinal plants and food supplement that contain medicinal plants. Experimental data indicate that polyphenols exhibit biological functions, as protection against oxidative stress and degenerative diseases. For identification of polyphenolic compounds in plants high-performance thin layer chromatography (HPTLC) is one of the simple and accurate method, which provide important information regarding chemical composition. The chromatographic polyphenolic fingerprint of the four species analysed (Allium sativum - garlic- green leaves, Allium ursinum - wild garlic - green leaves, Malus pumila - apple tree - leaves, Pyrus communis - pear tree - leaves) revealed the present of flavonoid glycosides and hydroxycinnamic acids compounds. Because for all the four species for nutritional and therapeutic purpose are used other part of them (garlic and wild garlic- bulbs and apple and pear-fruits) the results obtained can be a first step for superior valorification of hole plant.
 
Analysis of Tissue Phantom Ratio of the Megavoltage Photon Beams
Full text linkIso-centric beam data, phantom tissue ratios (TPR) are a dosimetric quantity commonly used to describe the change in dose with depth in tissue. Measurement of this is time-consuming and has the possibility of lose the consistency. The value of this quantity of any filed size in any depth is possible to calculate conveniently by the newly developed formula using only percentage depth dose (PDD) data of two fields. PDD for square fields ranging from 2 to 30 cm and various depths in increment of 0.4 cm up to maximum 30 cm have been measured in water at a fixed source surface distance (SSD) of 90 cm for 4, 6 and 15 MV photon beams in Ahsania Mission Cancer & General Hospital (AMCGH), Dhaka, Bangladesh. TPR values calculating for these energies of the same field sizes, depths and SSD by using the developed formula compared with those determined from the measured PDD data using a standard formula and had the good agreement. Mean error less than 1% observed between these TPR values.
 
Protective Effects of Ferulic Acid in Alcohol Withdrawal Induced Anxiety and Depression in Mice
Full text linkBackground: Anxiety and depression are the most important troubling symptoms of continuous alcoholism. Objective: The present study was designed to examine the protective effects of ferulic acid in alcohol withdrawal-induced anxiety and depression in experimental mice.
Methods: Male albino mice were divided into different groups. They were received 10% ethanol (2 g/kg; p.o.) twice on the first day and once on successive days for total six days, after 24 hrs. Withdrawal symptoms were observed using the different model for anxiety and depression such as elevated plus maze, open field test, hole board test, marble burying test and tail suspension test. Ferulic acid was tested as 10 and 20 mg/ kg, orally.
Results: Treatment with ferulic acid (10 and 20 mg/kg, p.o) showed significant reduction of alcohol withdrawal syndromes in different models. Taken together our result showed a protective effect in alcohol withdrawal anxiety and depression as tested in well-established animal models.
Conclusion: The present study showed that ferulic acid dose-dependently prevents alcohol withdrawal-induced anxiety and depression in mice