Revista Jurídica Digital UANDES
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Microfluidic separation coupled to mass spectrometry for quantification of peanut allergens in a complex food matrix
No full textPeanut is an important food allergen but cannot currently be reliably detected and quantified in processed foods at low levels. Three mg protein/Kg is increasingly being used as a reference dose above which precautionary allergen labeling (PAL) is applied to food products. Two exemplar matrices (chocolate dessert and chocolate bar) were prepared and incurred at 0, 3, 10 or 50 mg/Kg peanut protein using a commercially available lightly roasted peanut flour ingredient. After simple buffer extraction employing an acid labile detergent, multiple reaction monitoring (MRM) experiments were used to assess matrix effects on detection of a set of seven peptide targets derived from peanut allergens using either conventional or microfluidic chromatographic separation prior to mass spectrometry. Microfluidic separation provided greater sensitivity and increased ionisation efficiency at low levels. Individual monitored transitions were detected in consistent ratios across the dilution series performed, independent of matrix. The peanut protein content of each sample was then determined using ELISA and the optimised MRM method. Whilst other peptide targets were detected with three transitions at the 50 mg/Kg peanut protein level in both matrices, only Arah2(Q6PSU2)147-155 could quantify reliably, and only in the chocolate dessert at 10 mg/Kg peanut protein. Recoveries were consistent with ELISA analysis returning around 30-50% of the incurred dose. MS coupled with microfluidic separation shows great promise as a complementary analytical tool for allergen detection and quantification in complex foods using simple extraction methodology
Measuring the people fleet: general analysis, interventions and needs
No full textPurpose – The purpose of this paper is to deepen conceptual understanding of how employee wellbeing is identified and categorised in the workplace, and how management information is used to target workplace interventions.Design/methodology/approach – This is a conceptual paper reviewing and discussing contemporary literature and practice, with a focus on themes congruent with employee needs and organisational intervention options in relation to wellbeing. This paper considers wellbeing in the context of police work in the UK, and how a framework can help those charged with leading to understand and act in the interest of both the employee and the organisation.Findings – This paper suggests that the use of an appropriate strategic HR model, such as the General Analysis, Interventions and Needs (GAIN) pyramid (Hesketh and Rhodes, 2015), can assist organisations to develop practical categories and metrics to illustrate employee status in relation to wellbeing.Practical implications – The arguments posed provide opportunities for practitioners to use workforce-modelling tools that assist in identifying, categorising and targeting wellbeing interventions in the workplace.Originality/value – This paper highlights that identifying, categorising and prioritising wellbeing interventions in the workplace has hitherto received little academic attention. This paper contributes by providing a greater practical insight into what may work, which is important for leaders in all organisations, particularly those trying to maintain operational performance whilst undergoingprogrammes of change
Effects of Water Chemistry and Metal Ion Concentrations on the Cobalt Incorporation into Austenitic Stainless Steel Surface Oxide under Boiling Water Reactor Conditions.
No full textDeveloping a framework to motivate consumers and other stakeholders into becoming agents of sustainable development
No full textEven though the concepts of sustainable development and sustainability are more than 30 years old, the success of these ideas still lags behind expectations of what promise sustainable futures held for progress by the second decade of the 21st century. Involving consumers in the development of new sustainable products could be the key to the success of sustainability; sustainable brands adding value via engaging consumers in product journeys and leading the way in sustainable education could be the solution.This paper proposes a theoretical and practical framework to facilitate the exchange of such information and improve the interaction between companies, consumers and other stakeholders in matters of sustainability. A model was designed based on a thorough review of the literature and a comparison of propositions on consumer involvement approaches described in detail in the previous work of Moreira et al (2015). From the analysis of 29 different approaches on consumer involvement a new product development framework is proposed, providing indicators for the development of sustainable products and services more likely to succeed in today’s competitive market. Finally, to ease the framework’s implementation, three activities are proposed for future applications: workshops, an educational programme and an internet based system consolidating the relationship between companies and consumers.<br/
The Twilight of the Polls? A Review of Trends in Polling Accuracy and the Causes of Polling Misses
No full textPolls have had a number of high profile misses in recent elections. We review the current polling environment, the performance of polls in a historical context, the mechanisms of polling error, and the causes of several high profile misses in Britain and the United States. Contrary to conventional wisdom, polling errors have been constant over time, although the level of error has always been substantially beyond that implied by stated margins of error. Generally there is little evidence that voters lying about their vote intention (so called Shy Voters) is a substantial cause of polling error. Instead polling errors have most commonly resulted from problems with representative samples and weighting, undecided voters breaking in one direction, and to a less extent correctly calibrating turnout models and late swing. We conclude with a discussion of future directions for polling both in terms of fixing the problem identified and new approaches to understanding public opinion
