Notulae Botanicae Horti Agrobotanici Cluj-Napoca
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Comparative analysis of genetic diversity in Norway spruce (Picea abies ) clonal seed orchards and seed stands
Norway spruce, Picea abies (L.) Karst. is the most important conifer species in Romania and the mostplanted tree species in the Carpathian Mountains. Here we compare the genetic diversity of four Norwayspruce clonal seed orchards and two seed stands located in the Eastern Carpathians. A set of highlypolymorphic nuclear microsatellite markers was used. The analysis of genotypic identity of ramets for eachNorway spruce clone in all seed orchards indicated that nearly all sampled ramets (97%) were geneticallyidentical. The genetic diversity in seed orchards (He=0.700) was slightly smaller compared to the seed stands(He=0.718). Allelic richness was higher in seed stands (10.874), compared to clonal seed orchards (8.941).The Bayesian analysis indicated a genetic structure with two clusters, one corresponding to the clonal seedorchards and a second one consisting of the two seed stands. Our results provide valuable information for themanagement of Norway spruce seed orchards in Romania
Amelioration of water deficiency stress in roselle (Hibiscus sabdariffa) by arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria
Belowground interactions between plant roots, arbuscular mycorrhizal fungi (AMFs), and plant growth-promoting rhizobacteria (PGPR) can improve growth and yield under abiotic stress conditions. A pot factorial experiment based on completely randomized design with three replications was conducted to investigate the effects of AMFs (without inoculation as control, inoculation with Funneliformis mosseae and Funneliformis intraradices) and PGPRs (without inoculation as control Pseudomonas fluorescens p-169 inoculation) on roselle (Hibiscus sabdariffa L.) grown under water deficiency stress (WDS) [90% (I1), 75% (I2), 50% (I3), and 25% (I4) of field capacity as well-watered, mild, moderate, and severe stress, respectively]. The results showed that by applying WDS, the plant growth properties such as root and sepals’ dry weight, 1000-seed weight, seed yield, chlorophyll a, b, and total, carotenoids, and leaf water content was significantly reduced. The application of AMFs and PGPR under WDS conditions increased 1000-seed weight, seed yield. In response to WDS osmotic adjustment were provided in Roselle and under stress conditions. The highest seed yield was found under well-watered treatment by inoculation of F. mosseae without PGPR and the application of Pseudomonas fluorescens (6.37 and 6.51 g/plant, respectively). These results suggesting the antagonistic effects of AMFs and PGPR. AMFs inoculation under severe stress increased sepals dry weight compared to the non-inoculation. In conclusion, increased activity of enzymatic antioxidants and higher production of non-enzymatic antioxidant compounds, as well as photosynthetic pigments in symbiotic association with AMFs, can alleviate reactive oxygen species damage resulting in increased growth and yield parameters and improve water stress tolerance
The importance of Rhizobium, Agrobacterium, Bradyrhizobium, Herbaspirillum, Sinorhizobium in sustainable agricultural production
Rhizobia which are soil bacteria capable of symbiosis with legume plants in the root or stem nodules and perform nitrogen fixation. Rhizobial genera include Agrobacterium, Allorhizobium, Aminobacter, Azorhizobium, Bradyrhizobium, Devosia, Mesorhizobium, Methylobacterium, Microvirga, Ochrobacterum, Phyllobacterium, Rhizobium, Shinella and Ensifer (Sinorhizobium). Review of the literature was carried out using the keywords Rhizobium, Agrobacterium, Bradyrhizobium, Herbaspirillum and Sinorhizobium. Rhizobial nodulation symbioses steps are included flavonoid signaling, Nod factor induction, and Nod factor perception, root hair responses, rhizobial infection, cell division and formation of nitrogen-fixing nodule. Rhizobium improves sustainable production by boosting organic nitrogen content
Influences of sea water on the ethylene-biosynthesis, senescence-associated gene expressions, and antioxidant characteristics of Arabidopsis plants
We evaluated the physiological and antioxidant characteristics of Arabidopsis thaliana (At) plants grown in different sea water (SW) products containing trace elements, namely RO3, 300K, and 340K, at various dilutions. The synthetic water (namely 300K-Test), a mixture of the main ions of SW including 143.08 mg L-1 Mg2+, 5.74 mg L-1 Na+, 170 mg L-1 K+, and 33.5 mg L-1 Ca2+ with equal concentrations to those in 300K SW without trace elements, was also used to culture At plants and study the influences that the major ions had on regulating ethylene production. The ethylene-biosynthesis (ACS7 and ACO2) and senescence-associated (NAP, SAG113, and WRKY6) gene expressions in SW- and ionic-treated At plants in response to transcriptional signaling pathways of ethylene response mechanisms were also investigated. Our results show that down-regulation of the ACS7 gene in 300K-treated plants significantly reduced the ethylene content but remarkably increased chlorophyll, total phenol, and DPPH radical scavenging accumulations and strengthened the salt tolerance of 300K-treated plants. The expression of the ACS7 gene of At plants under 300K, Ca2+, Mg2+, and Na+ treatments was correlated with decreases in NAP, SAG113, and WRKY6 gene expressions. The application of Ca2+ increased total phenol content and reduced the accumulation of superoxide, which in combination decreases plant aging brought on by ethylene. However, K+ treatment inhibited SGA113 gene expression, resulting in reducing ACS7 gene expression and ethylene content. The characterization and functional analysis of these genes should facilitate our understanding of ethylene response mechanisms in plants
An analysis of the profitability of the restitution of sessile oak forests affected by wildfires in Serbia
Wildfires caused by climate change in Europe have numerous and long-lasting ecological, economic, and social implications for sustainable development (natural environment, economy, and society as a whole). Artificial revitalization of sessile oak forests destroyed by wildfires in Serbia is feasible, but it is quite expensive compared to the spontaneous regeneration. Cost-benefit analysis (an economic approach to comparing and estimating the benefits and shortcomings of a particular business plan, i.e., an investment project by analyzing its costs and benefits) is aimed at finding the best solutions and making the best decisions about the desirability of a project. It was concluded that the investment in the restitution of sessile oak forests could be profitable only if it was based on wood production. Wood production may bear slightly higher interest rates compared to previous estimates which ranged mainly around 3%. This primarily applies to better site classes, while poor quality sites require lower rates. At lower discount rates, it takes more time to reach the break-even point, while the period of time needed to reach the break-even point shortens with higher discount rates. The length of the production cycle plays an important role in determining the investment profitability, especially regarding the relationship between the length of the production cycle and production targets (the type and the quality of wood assortments produced). According to the results of the analysis of intangible benefits and costs, the costs outweigh the benefits, which makes the restitution of sessile oak forests destroyed by fires unacceptable
Potential of vegetable soybean cultivation in Lithuania
The objective of the present study was to investigate the potential of vegetable soybean (Edamame) cultivation in Lithuania. Six soybean varieties were grown in an open field. Our data showed that the plants of the ‘Chiba Green’ variety were the shortest. Such plants had higher chlorophyll index and photosynthesis intensity. Variety ‘Midori Giant’ had more branches than plants of other varieties. The highest yield was produced by the plants of the ‘Chiba Green’ variety. Higher protein content was found in beans of the ‘Chiba Green’ variety. However, there were no differences found in soluble carbohydrates and micro- and macroelement contents between the varieties. In summary, the results revealed that of the tested varieties, the most suitable and useful for the cultivation under Lithuanian climate conditions was the ‘Chiba Green’ vegetable soybean variety
Foliar application of zinc oxide nanoparticles and grafting improves the bell pepper (Capsicum annuum L.) productivity grown in NFT system
The bell pepper (Capsicum annuum L.) is a food vegetable with a high nutritional intake, with rich content in vitamins, minerals and antioxidants. In this study, using nutrient film technique (NFT) system, the effect of the zinc oxide nanoparticles on the micromorphology, histology, physiology and production of the grafted pepper was evaluated. The treatments used were grafted and non-grafted plants, four concentrations (0, 10, 20, 30 mg L-1) of zinc oxide nanoparticles, and the experience was organized in a completely randomized design. An increase in grafted plants was observed in the weight, number and size of fruits in 18.1%, 21.8% and 9.6%, the concentration 30 mg L-1 of nanoparticles statistically affected the weight, number and size 46.9%, 47.7% and 18% compared to the control. The interaction with grafted plants and the treatment of 30 mg L-1 of zinc oxide nanoparticles increased fruit weight, number of fruits and size by 62.60%, 57.69% and 29.17% compared to plants without grafting and the control treatment. These results indicate that the use of grafts and zinc oxide nanoparticles could be used in bell pepper production to increase yield
Foliar application of melatonin alleviates the effects of drought stress in rice (Oryza sativa L.) seedlings
Melatonin (N-acetyl-5-methoxytryptamine) plays an essential role in abiotic stress in plants, but its mechanism in drought tolerance is unclear. To better understand the protective roles of melatonin against drought stress, we investigated the effect of foliar application with exogenous melatonin on plant growth, physiological responses, and antioxidant enzyme activities in rice seedlings under drought stress. Rice seedlings were grown for 21 days, and foliar sprayed with 0, 50, 100, 200, and 300 µM melatonin. The control plant was watered daily, while the others were subjected to drought stress by withholding water for seven days. The results showed that drought stress significantly reduced plant growth, relative water content, and chlorophyll content. Electrolyte leakage, malondialdehyde (MDA) content and hydrogen peroxide (H2O2) were also negatively affected by drought stress. Application of melatonin alleviated the effects of drought stress by increasing plant growth, improving relative water content and chlorophyll content, and decreasing electrolyte leakage, MDA, and H2O2. Foliar application with melatonin also increased antioxidant enzyme activities, including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (GPX). In addition, melatonin also enhanced proline and total soluble sugar accumulation during drought stress. It is, therefore, suggested that foliar application with 100 µM melatonin was the most effective for reducing the adverse effects of drought stress in rice plants
Comparative study of the effect of salt stress, Alternaria alternata attack or combined stress on the Cakile maritima growth and physiological performance
Cakile maritima is a halophytic plant model that is well known by its ability to tolerate high salt concentrations. Salinity was reported to improve the tolerance of halophytes to several abiotic stresses; however, the involvement of salt in the tolerance to biotic stress is still scant. In the present work, the effect of salt on C. maritima responses towards the pathogenic Alternaria alternata was investigated. For that, C. maritima seeds were germinated for four weeks. Plants were then divided into four groups: i) Plants irrigated with salt (200mM NaCl); ii) Plants infested by fungus; iii) Plants irrigated with salt and infested by fungus and finally control plants (0mM NaCl, without inoculation). Our results showed that upon salt stress or fungal attack, plants reduced biomass production, hydration status and photosynthetic performance which were associated with a decrease in the gas exchange and chlorophyll fluorescence parameters, with a more pronounced effect upon fungal attack. However, under combined stress, a significant increase of these parameters was noticed, with a level close to that of control. Concerning nutrient contents, K, Zn, Fe, Cu and Mg decreased in the C. maritima leaves exposed to both stresses applied individually. In contrast, all these nutrients were increased in plants grown under combined stress. Taken together, we can conclude that plants grown under combined stresses had better growth rate and physiological performance compared to all other treated plants, and that salt may be the key in improving the C. maritima ability to tolerate fungal attack
Introduction pages
The papers published in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Issue 3, Volume 49, 2021 represent new exciting researches in different topics of life science, respectively in plant science, horticulture, agronomy and crop science. Among the interesting articles we invite you to find news about: Recent advancements on use of CRISPR /Cas9 in maize yield and quality improvement; Genome-wide investigation of Hydroxycinnamoyl CoA: Shikimate Hydroxycinnamoyl Transferase (HCT) gene family in Carthamus tinctorius L.; Identification of SNPs in rice GPAT genes and in silico analysis of their functional impact on GPAT proteins; Extensive transcriptome changes underlying the fruit skin colour intensity variation in purple eggplant; Physiological variation of irradiated red radish plants and their phylogenic relationship using SCoT and CDDP markers; Alterations in leaf anatomy, quality, and quantity of flavonols and photosynthetic pigments in Nigella sativa L. subjected to drought and salinity stresses; Impact of the foliar application of nanoparticles, sulfate and iron chelate on the growth, yield and nitrogen assimilation in green beans; Phytotoxicity and allelopathic potential of extracts from rhizomes and leaves of Arundo donax, an invasive grass in neotropical savannas; Comparative study of the effect of salt stress, Alternaria alternata attack or combined stress on the Cakile maritima growth and physiological performance, etc. The Impact Factor communicated by ISI Clarivate (June 30, 2021), is IF 2020 = 1.444 (position 157 of 235 journals, Q3 in Plant Sciences). The metrics in Scopus – Elsevier (June 2, 2021): CiteScore 1.7 (#40/86 in Horticulture)