Notulae Botanicae Horti Agrobotanici Cluj-Napoca
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Water-yield relationship of greenhouse-grown strawberry under limited irrigation
Get PDFIn this study, the traditional full/deficit irrigation and partial root drying (F-PRD) techniques with the four different amounts of irrigation (100%, 80%, 60% and 40%) were examined on Rubygem Strawberry species under controlled greenhouse conditions, in terms of yield, yield parameters, WUE, IWUE, yield response factor (ky), etc. and their possible reactions have been investigated. In the study in which fresh seedlings were used, planted on the rows in a triangular form at intervals of 20×20 cm in double rows. Irrigation applications were carried out by drip irrigation method and the amount of irrigation water in the control treatment (I100) was calculated using measurements taken from an A-Class evaporation pan. Eight irrigation treatments including four traditional irrigation (I100, I80, I60, I40) and four fixed-partial root drying (F-PRD100, F-PRD80, F-PRD60, F-PRD40) were examined within the scope of the study. In traditional irrigation (I100, I80, I60, I40) applications, both sides of the plant root zone were irrigated. However, during the entire growing season in the fixed-partial root drying (F-PRD100, F-PRD80, F-PRD60, F-PRD40) technique, one-half of the plant root zone was kept wet, and the other half was left relatively dry. The yield values were ranked as, from high to low, F-PRD100>I100>F-PRD80>I80>F-PRD60>I60>I40>F-PRD40. In irrigation treatments, the yield values obtained from I80 and F-PRD80 treatments were statistically similar (Duncan 5%) to the yield values of I100 and F-PRD100 treatments. However, it could be stated that the F-PRD80 is more advantageous. It was found that different amounts of irrigation water (100%, 80%, 60% and 40%) affects the yield and yield-related parameters obtained from the strawberry plants. In general, as the water deficit increased (in the I40 and F-PRD40 treatments), the level of negative impact also increased. As a result of the research, F-PRD80 was proposed for the cultivation of strawberries, particularly, in arid and semi-arid regions, depending on the availability of water
The growth rate and genetic variability of Scots pine (Pinus sylvestris L.) half-sibs in test crops of Northern Kazakhstan
Get PDFScots pine (Pinus sylvestris L.) is a valuable tree species, playing an important role in maintaining the ecological and economic balance not only in Kazakhstan, but also in many countries around the world. The purpose of this study was to assess the growth rate and to identify the genotypes of the half-sib progeny of Scotch pine plus trees in order to further obtain high-quality seeds with valuable genetic traits. A molecular genetic analysis was carried out using six microsatellite and 20 isoenzyme loci on 15 Scots pine half-sib families, represented by two samples of seeds collected in the subsequent harvesting periods (planted in 1986 and 1987), that evaluated the taxonomic characteristics such as tree height and trunk diameter (DBH, measured at 1.3 meters). Families with the highest growth rate in both samples were selected as candidates for the elite group. During the SSR analysis, a decrease in the level of expected heterozygosity (0.2377-0.5362 with an average value of 0.4593) was observed in P. sylvestris L. half-sibs compared to the sample of maternal plus trees (0.5444), with similar results to that of the “founder effect”. At the same time, the level of observed heterozygosity remained sufficiently high (0.3333-0.7037 with an average value of 0.4980 and 0.4556, respectively). During the isoenzyme analysis, the level of genetic variability either decreased or was similar to that of the maternal trees. Rare and unique alleles were found in seed samples of the analysed tree families. In general, this research showed that a comprehensive evaluation combining both traditional breeding methods (genetic assessment of progeny in the course of growth) and molecular genetic analysis is required for the selection of elite trees
Chemical composition of Tithonia diversifolia (Hemsl.) and its effect on growth performance, feed efficiency and metabolic biochemistry of juvenile hybrid tilapia Oreochromis mossambicus × Oreochromis niloticus
Get PDFThe aquaculture due to the high cost and continued shortage of animal protein sources, the development of alternative plant protein sources has been one of the main challenges to establish sustainable aquaculture as economically viable. Objective of this investigation was to determine the chemical composition and effect of Tithonia diversifolia on the growth performance, feed efficiency and metabolic biochemistry of juveniles of Oreochromis mossambicus × Oreochromis niloticus. Five treatments were used: 0% (control), 4, 8, 12 and 16% levels of inclusion of T. diversifolia flour in diet to feed juvenile fish cultured in 15 plastic aquariums at a density of 15 fish/aquarium. The assay lasted eight weeks. The T. diversifolia flour was characterized for showing adequate levels of crude protein (21%), with low crude fat (4.5%) levels, neutral detergent fiber levels: 43.03% and acid detergent fiber: 24.40%. The growth performance presented significant differences (p˂0.05), for weight gain, day average weight gain (DAW), length gain, day average length gain (DAL), specific growth rate (SGR), feed efficiency (FE), protein efficiency ratio (PER) and feed conversion ratio (FCR). In treatment with 16% of inclusion of T. diversifolia flour, values obtained for weight gain, DAW, SGR, FE and PER (15.88 g, 0.28 g. day-1, 2.03 %, 0.34 g.g-1 and 0.98, respectively), which did not differ with the control treatment, highlighting the benefits of T diversifolia flour. The metabolic biochemistry parameters experienced a decrease with the increase of T. diversifolia in diet, where the highest values of triglycerides, cholesterol, and glucose were for the control treatment (p <0.05), while the protein increased to 4% of the flour. T. diversifolia flour presents an adequate balance of nutrients for its chemical composition and amino acids, without causing negative effects on the growth performance, weight-length relationship, feed efficiency, survival and metabolic biochemistry
Effect of cultivar, year, and their interaction on nutritional and energy value components in Aronia melanocarpa berries
Get PDFAronia melanocarpa has long been known for its small, purple-black, and sweet-bitter berries to which several human health benefits have been attributed. This study aimed to highlight the effect of cultivar, year, and their interaction on the berry level of some components with nutritional and energetic value in two chokeberry cultivars. The study was carried out on ‘Melrom’ and ‘Nero’ chokeberry cultivars, grown under climatic conditions specific to Muntenia region, Romania. Compared to ‘Nero’ cv., dry weight, total soluble solids, and carbohydrates were found in significantly higher quantity in ‘Melrom’ cv. (263.33 g kg-1, 18.02 °Brix, 203.19 g kg-1). Significantly higher energy value and acidity (946.81 kcal kg-1 and 11.40 g malic acid kg-1, respectively) were also determined for ‘Melrom’ cv. Except for total soluble solids for ‘Melrom’ cv., total titratable acidity and total sugar content presented the highest variations between the study years for each of the two cultivars. K was the major mineral, followed by Ca. ‘Nero’ cv. had a higher content of K (2.73 g kg-1) and Zn (0.003 g kg-1) and significantly higher levels of Ca (0.72 g kg-1), Mg (0.35 g kg-1), and P (0.52 g kg-1). Both chokeberry cultivars can be considered as an alternative to complete the daily diet with fiber and minerals without high energy or sugar intake
Efficacy of the application of boron nanofertilizer on biomass, yield, nitrogen assimilation and photosynthetic activity in green beans
Get PDFBoron (B) nanofertilizers are an innovative alternative with great potential to make nutrient application more efficient and thereby improve crop growth and productivity. However, nowadays there is little literature on the effects of boron nanofertilizers on physiological and biochemical processes in plants. Therefore, the objective of the present research was to study the efficacy of foliar application of a boron nanofertilizer on biomass, yield, nitrogen assimilation and photosynthetic activity in green beans plants cv. Strike. The B nanofertilizer was foliar applied at 0, 25, 50 and 100 ppm. Biomass accumulation, yield, nitrate reductase enzyme activity, photosynthetic activity, stomatal conductance and photosynthetic pigment content were evaluated. The results obtained indicate that the application of B nanofertilizer at dose of 25 ppm were more effective in improving biomass, while the dose of 100 ppm favoured nitrate reductase activity and stomatal conductance. The results suggest that the application of B nanofertilizers stimulated the development of green bean plants. Finally, more studies are needed to evaluate the possible phytotoxic effects of high doses of B nanofertilizers and to compare their effects with conventional B fertilizers already used in the market
Rice grain yield and quality improvement via CRISPR/Cas9 system: an updated review
Get PDFRice (Oryza sativa L.) is an important staple food crop worldwide. To meet the growing nutritional requirements of the increasing population in the face of climate change, qualitative and quantitative traits of rice need to be improved. During recent years, genome editing has played a great role in the development of superior varieties of grain crops. Genome editing and speed breeding have improved the accuracy and pace of rice breeding. New breeding technologies including genome editing have been established in rice, expanding the potential for crop improvement. Over a decade, site-directed mutagenesis tools like Zinc Finger Nucleases (ZFN), Transcriptional activator-like Effector Nucleases (TALENs), and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) System were used and have played a great role in rice yield and quality enhancement. In addition, most recently other genome editing techniques like prime editing and base editors have also been used for efficient genome editing in rice. Since rice is an excellent model system for functional studies due to its small genome and close synthetic relationships with other cereal crops, new genome-editing technologies continue to be developed for use in rice. Genomic alteration employing genome editing technologies (GETs) like CRISPR/Cas9 for reverse genetics has opened new avenues in agricultural sciences such as rice yield and grain quality improvement. Currently, CRISPR/Cas9 technology is widely used by researchers for genome editing to achieve the desired biological objectives, because of its simple targeting, easy-to-design, cost-effective, and versatile tool for precise and efficient plant genome editing. Over the past few years many genes related to rice grain quality and yield enhancement have been successfully edited via CRISPR/Cas9 technology method to cater to the growing demand for food worldwide. The effectiveness of these methods is being verified by the researchers and crop scientists worldwide. In this review we focus on genome-editing tools for rice improvement to address the progress made and provide examples of genome editing in rice. We also discuss safety concerns and methods for obtaining transgene-free crops
Microbial diversity and physicochemical properties in farmland soils amended by effective microorganisms and fulvic acid for cropping Asian ginseng
Get PDFDemand for products made from the dry mass of Asian ginseng (Panax ginseng) is growing, but harvest is limited by fungal disease infection when ginseng is replanted in the same field. Rotated cropping with maize can cope with the replant limit, but it may take decades. We aimed to amend post-maize-cropping farmland soils for cultivating Asian ginseng, using effective microorganisms EMs and fulvic acid (FA) additives and detecting and comparing their effects on soil microbial diversity and physiochemical properties. Amendments promoted seedling survival and depressed disease-infection. Both EMs and FA increased the relative abundances of Pseudomonas, Flavobacterium, Duganella, and Massilia spp., but, decreased the relative abundances of Fusarium and Sistotrema. In addition, soil nutrient availability and properties that benefitted nutrient availabilities were promoted. In conclusion, amendments with EMs and FA improved the fertility of farmland soils, and the quality of Asian ginseng, and revealed the relationship between soil microbial diversity and physiochemical properties
Microbiome structure and response to watering in rhizosphere of Nitrosalsola vermiculata and surrounding bulk soil
Get PDFThe plant rhizosphere microbiomes were thought to help the plant stands adverse condition. The study aims at deciphering signatures of rhizosphere soil microbiomes of the medicinal plant Nitrosalsola vermiculata and those of the surrounding bulk soil as well as to detect influence of watering in restructuring soil microbes that can improve the plant’s ability to tolerate drought stress. Amplicon sequencing of partial 16S rRNA gene indicated that alpha diversity indices are higher in rhizosphere than in bulk soils, while no distinctive differences were observed due to the watering. Relative abundance of phylum Cyanobacteria and its descendent unidentified genus is the highest among phyla and genera of bulk soil. Relative abundance of phyla Euryarchaeota, Chloroflexi, Actinobacteria, Proteobacteria, Bacteroidetes, Firmicutes, Acidobacteria and Gemmatimonadetes as well as genera Bacillus, Ammoniphilus, Sphingomonas, Microvirga, Pontibacter, Adhaeribacter and Arthrobacter was significantly higher in rhizosphere soil. The latter taxa were reported to act as plant growth-promoting bacteria (PGPB) through symbiotic associations. We speculate that relative abundance and mutual dominance of these taxa in rhizosphere of N. vermiculata were due to the intensity and type of plant root exudates. Other factors include soil pH where microbes favoring high soil pH can show better growth in rhizosphere soil. Also, co-existence of phyla that promote sustainability of cohabiting phyla in the rhizosphere and have high synergism prevalence in biofilm formation can be one extra factor. Quorum sensing (QS) also mediates bacterial population density in a given environment and elicit specific plant responses. The low abundance of Cyanobacteria in rhizosphere soil can be due to the inhibitory effect of highly abundant members of Firmicutes, especially those of genus Bacillus. The latter conclusion was confirmed by the occurrence of high expression rate of comQ gene triggering QS in genus Bacillus. Highly abundant microbes whose abundance was not changed due to watering are phyla Firmicutes, Proteobacteria, Chloroflexi and Cyanobacteria and their descendent genera Bacillus, Ammoniphilus, Sphingomonas, Microvirga and unidentified genus of Cyanobacteria. We speculate that non-responsive taxa to watering were drought tolerant and can help plants stand adverse conditions of water scarce. In conclusion, insights on the factors involved in shaping microbiome signatures and those eliciting differential plant responses to drought stress are raised and warrant further investigations
Performance of fourteen genotypes of durum wheat under Eastern Mediterranean conditions
Get PDFDurum wheat is used as raw material for many foods. Climate change might be responsible for larger or smaller changes in crop yields. For the combined assessment of climate and crop, growing degree days (GDDs) have a crucial role. Two experimental lines and twelve commercial wheat (Triticum durum) varieties from diverse backgrounds were cultivated to compare their crop properties, yield, and protein content in terms of GDDs. The experiment was established in typical Mediterranean environment, using a randomized complete block design with blocks of varieties and lines for two growing seasons. For all varieties, GDDs to head emergence was affected by factor year, whereas GDDs from head emergence to harvest were influenced by both varieties and year. Protein content (%) was not affected by genotypes. Factor of variety and interaction variety × year had an impact on vitreousness; it was ranged from 79.75 % (‘Makaras’ variety) to 44.00 % (‘Levante’ variety). Yield had no statistically significant difference among varieties/lines. In durum wheat cultivation, up to head emergence, when GDDs increased, yield would be declined in contrast to GDDs from emergence to harvest; with the increasing of GDDs to harvest, yield was climbed. Nowadays, the integrations of and interpretation of GDDs in the evaluation of crop performance seem vital
Assessment of the virus infections occurrence in new established plum and sweet cherry orchards in Transylvania, Romania
No full textSixteen plum and eleven sweet cherry newly established commercial orchards in Transylvania, Romania, were the subject of the survey during the vegetative periods of 2020-2021 in order to assess the occurrence of different virus infections. Two blocks of 200 trees from each orchard were monitored by visual observation for virus-like symptoms, especially for Plum pox virus (PPV) in plum orchards. Twenty trees of each plum orchard were then sampled and tested for serological detection of PPV, Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV), Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV) and Myrobalan latent ringspot virus (MLRSV). Similarly, ten trees of each sweet cherry orchard were sampled and tested for the presence of PDV, PNRSV, ApMV, ACLSV, PPV, Arabis mosaic virus (ArMV), Cherry leaf roll virus (CLRV), Raspberry ringspot virus (RpRSV), Strawberry latent ringspot virus (SLRSV) and Tomato black ring virus (TBRV) by serologic assays. Additionally, a few sweet cherry trees suspected to be infected by Little cherry virus-1 (LChV-1) were tested by molecular assay. Unexpectedly, no plum orchards were found to be free of PPV. The average level of PPV infection was 32%. PNRSV occurred in 8.1% and PDV in 1.2% of sampled plum trees. Four out of ten viruses were detected in sweet cherry sampled: PDV (3.6%), ACLSV (0.9%), RpRSV (0.9%) and TBRV (0.9%). No infection with ACLSV, ApMV and MLRSV in plum and PPV, PNRSV, ApMV, ArMV, CLRV, SLRSV and LChV-1 in sweet cherry was detected. Mixed infections occurred at 4.8% in plum, and at 0.9% in sweet cherry trees sampled. The average occurrence of viruses in plum and sweet cherry orchards surveyed in Transylvania was determined at 41.3%, and 6.3% respectively. Overall results revealed a critical situation especially in regards to PPV infections making the success of fruit production in the most surveyed plum orchards quite problematic. Virus infections level in the younger sweet cherry orchards were significantly lower