Notulae Botanicae Horti Agrobotanici Cluj-Napoca
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Gene mining and functional analysis related to maize (Zea mays L.) seed size
Get PDFMaize has widely been studied as a model of plant-growth promoting rhizobacteria (PGPR). Here, the genome sequences of 9P. The strains, together with 26 other sequenced Maize were comparatively studied. Phylogenetic analysis of the concatenated 244 single-copy core genes suggests that the 9P. The strains and 5 other Paenibacillus spp., isolated from diverse geographic regions and ecological niches, formed a closely related clade (here it is called Poly-clade). Analysis of single nucleotide polymorphisms (SNPs) reveals local diversification of the 14 Poly-clade genomes. SNPs were not evenly distributed throughout the 14 genomes and the regions with high SNP density contain the genes related to secondary metabolism, including genes coding for polyketide. Recombination played an important role in the genetic diversity of this clade, although the rate of recombination was clearly lower than mutation. The distinction among people and different creatures can be gotten by relative examinations. This study reveals that both maize and its closely related species have plant growth promoting traits and they have great potential uses in agriculture and horticulture as PGPR
Citron melon peel flours: drying kinetics and physicochemical evaluation
Get PDFIn the utilization of citron melon (Citrullus lanatus var. citroides), it is necessary to deal with a large amount of residues constituted by the peels. These materials commonly discarded can be fully utilized, since they are a source of nutrients. The peels contain not only fibers, but also proteins, sugars and minerals, which, after drying, are concentrated to values that make them interesting for various uses, including for the enrichment of flours or combinations to prepare bakery products. Therefore, the drying of the peels, besides enabling the conservation and storage at room temperature, expands the forms of use and the possibilities of entering the production chain. This study aimed to characterize physico-chemically and determine drying kinetics, effective diffusivity, activation energy and thermodynamic properties of citron melon peels at different drying temperatures. The peels were dried in an oven with forced air circulation, in a thin layer, at temperatures of 60, 70, 80 and 90 °C. With the data collected in the drying, the drying kinetic curves were constructed and eleven mathematical models were fitted to the experimental data. The dried material was crushed in a knife mill and characterized for physicochemical parameters. Midilli model resulted in the best fits, followed by Page and Approximation of Diffusion models. Effective diffusivity increased with drying temperature; activation energy was obtained from the Arrhenius equation and was equal to 8.18 kJ/mol. Enthalpy and entropy were reduced with increased temperature, while Gibbs free energy increased
Ecotype effects on photosynthesis performance using A/PFFD among Pinus nigra Arn.
Get PDFThis study aimed to provide insights on intraspecific variability of photosynthesis performance of 19 provenances of black pine planted in a common garden. The experiment was conducted in an experimental trial located at Souiniet (the southern limit of its range) Photosynthetic capacity characterized by light-saturated net photosynthetic rates, associated light compensation points and apparent quantum yield was monitored by measuring the response of leaf gas exchange to light levels. Amax was the highest in provenances Puget Theniers (nigra ssp), Saint-Guilhem (salzmanni ssp), Marghese and les barres (laricio ssp). The lowest value was recorded in Olette of the subspecies salzmanni. Needles from two provenances Crimea (pallasiana ssp) and Les Barres (ssp laricio) revealed the highest apparent quantum yield (ɸ), followed by Brougatlès Ales (salzmanni ssp), Trenta Coste (ssp laricio), Les barres and Puget Theniers (nigra ssp). The lowest apparent quantum Yield was recorded in Laricio subspecies (Cosenza; Les barres and Bois frerot). The highest value of dark respiration (Rd) was shown in provenance Les barres (laricio ssp), while Grancia and Tavola of the laricio subspecies reported the lowest one. Provenances Tavola (laricio ssp) and Puget Theniers (nigra ssp) exhibited the highest LCP. The provenance Crimée (pallasiana ssp) and Aspromonto (laricio ssp) recorded the lowest values. The maximum values of photosynthesis are positively correlated with the total chlorophyll contents and Leaf Mass area. Our study illustrates that Photosynthesis performance showed a difference between 19 provenances of black pine; it seems that black pine photosynthetic performance is eco-typical independent
The floristic composition, morphometric and the adaptation of a new species: Malva subovata in the matorral of Algeria (Djbel fellaoucen)
Get PDFThis study is based on a ruderal species: Malva subovata = Lavatera maritima which should normally grow in the littoral, under maritime influence. Its presence in the matorrals is highlighted for the first time. as it has been found far from its natural habitat. The comparison of the different biological and morphological spectra shows the importance of the therophytes that confirms the phenomenon of degradation. This degradation shows a depletion of the floristic procession, especially concerning sylvatic species which have given way to ephemeral therophytes and chamæphytes. Despite the presence of a shrubby layer, it is no longer a question of a forest ecosystem but of a pre-forest ecosystem. To better understand the characteristics of this Malvaceae in terms of resistance and adaptation in matorrals, we often use the phytomass of all anthropozoic formations and more specifically the phytomass of the species that characterize this group. In our case, this is Malva subovata. The results of the morphometric study constitute a basic element for the evaluation of the adaptive potential of our Lavatère against the degradation factors
Utilization of jojoba oil and salicylic acid as postharvest treatment on storability and fruit quality of ‘Late Swelling’ peach cultivar
Get PDFThe ‘Late-Swelling’ peach is an important cultivar due to its attributes of fruit quality, such as handling ability and late harvest season. Peaches rapidly deteriorate during storage, which shortens their shelf-life. The preservation of quality traits of ‘Late-Swelling’ peach during handling and storage investigated. Fruits harvested in the early ripe stage (last week of June) were either dipped in jojoba oil (JO) 500, 1000, and 1500 ppm and salicylic acid (SA) 100, 200, and 300 ppm, then stored at 5+1 °C and 85% RH for 35 days besides 7 days as shelf-life the fruits were examined every 7 days until the end of the experiment. Positive influences were observed for JO and SA on the different chemical and physical characteristics of the peach fruits under study especially those related to storability and fruit quality. All fruits treated with JO 1500 and 1000 ppm, and SA 300 ppm maintained the fruit firmness, hº, total acidity, and ascorbic acid over the whole storage period, in addition to the lowest decay and weight loss, as well as maintaining the fruit sensory quality and decreased peroxidase [PPO] activity while increasing polyphenol oxidase [POD], catalase [CAT], and ascorbate peroxidase [APX] activities compared with the control at the end of shelf-life period. In conclusion, our results suggest using JO and SA to enhance and prolong the storability of peach fruits at 5+1 °C and 85% RH
Application of Trichoderma asperellum in apple trees as a growth regulator and antagonist for the control of Alternaria sp.
Get PDFChihuahua state is the main apple producer in Mexico. The present study aimed to evaluate the effectiveness of Trichoderma asperellum as a plant growth regulator and antagonist against Alternaria sp. Three treatments were used: T1 = control, T2 = 200 mL of T. asperellum per tree; T3 = 100 mL of T. asperellum per tree. Agronomic variables were evaluated including number of leaves, shoots and flowers, disease incidence, and trunk thickness. Alternaria sp. was isolated from apple leaves at the experimental site in Guerrero County, Chihuahua, Mexico, and it was grown on solid PDA medium for morphological characterization. The molecular characterization was done by PCR using primers ITS1 and ITS4 producing products of 700 bp which were sequenced, submitted to GenBank (acc. no. OQ344593) and used for further phylogenetic analysis through Bayesian inference approach. Three clades were identified and the polytome topography recovered from clade 2 indicates a high genetic similarity with A. tenuissima (100% similarity according to BLAST). The analysis of T. asperellum as growth regulator only showed significant differences in trunk thickness and displayed higher values with T3 (p≤0.05). The presence of A. tenuissima was only observed in the control, which indicated the ability of Trichoderma to control the fungus. In this study T. asperellum was not an efficient plant growth regulator, but it was a good antagonist, and hence it can be recommended to control A. tenuissima. This is the first record of A. tenuissima in apple trees in Mexico. These results indicate that T. asperellum showed no benefit as plant growth regulator when applied to apple trees of the ‘Granny Smith’ variety
Biodiversity of microbial populations as the indicator of biogenicity of soil under ashes and agricultural soil
Get PDFThe number, activity and diversity of microorganisms define the level of biogenicity and indicate the quality and health of the soil. The abundance and structure of microbial communities vary in different types of soils, so in order to preserve and protect natural and agroecological systems, in addition to physical-chemical analyses, monitoring of dynamics of microbiological activity in agricultural and non-agricultural soil is also necessary. Each type of soil has its own characteristic micro biocenosis, and different methods of soil use can have a positive or negative impact on microbiological activity, which directly affects the fertility of the soil. Soil bacteria are very important in biogeochemical cycles, and biological nitrogen fixation plays an important role in nitrogen cycling by transferring atmospheric dinitrogen into the soil. It is performed by symbiotic and asymbiotic nitrogen-fixing microorganisms, called nitrogen fixators. Amonificators are bacteria that play a very important role in soil ecosystem, as well as nitrogen fixators. This group of bacteria participates in the processes of protein decomposition and transformation. The aim of the paper is to indicate, based on the presence of nitrogen fixators and amonificators in soil under ashes, the biogenicity of this soil at four different localities: pure ashes, soil under ashes covered with herbaceous vegetation, soil under ashes covered with wood vegetation and agricultural soil, and to give advice on future activities regarding recultivation of these types of soil under ashes. The results of the research showed that microorganisms are mostly represented in different numbers in the tested soils, which indicates variations on the soil biogenicity and quality
Molecular study of pathogenic and saprophytic fungal species on infected parts of Malus pumila L. of district Qilla Abdullah, Balochistan, Pakistan
Get PDFApple (Malus pumila L) of the family Rosaceae, most cultivated fruit in temperate regions of the world and is used fresh or processed. The apple production is affected by several pathogens including fungi. The present study was designed to identify disease-causing agents that reduce fruit production in the district Qilla Abdullah of Balochistan, Pakistan, which is the main apple production area of the province. Three varieties of apple: ‘Tur-Kulu’ (‘Red Delicious’), ‘Shin-Kulu’ (‘Golden Delicious’), and ‘Kaja’ were selected. Infected leaf samples were collected from eight different sites of tehsil Gulistan, district Qilla Abdullah. The cultures of fungal micro-flora were grown on two media, potato dextrose agar (PDA), and malt extract agar (MEA) followed by incubation for one week. The resulting colonies were observed under a microscope and identified based on morphological characters. Predominant fungal species was identified through ITS marker and PCR amplification. The isolated pathogens belonged to Zygomycota and Ascomycota divisions. The pathogens found were Aspergillus niger, A. oryzae, A. terrus, Colletotrichum gloeosporioides, Fusarium oxysporum, Mucor spp., Penicillium expansum, and one species of Absidia as well as Rhizopus. Colletotrichum gloeosporioides were predominantly found in all varieties. Morphological and phylogenetic analysis confirmed the identity of Colletotrichum gloeosporioides. As a result of this study, the predominant pathogen species Colletotrichum gloeosporioides is one of the causes of leaf infection in apple varieties
Evaluation of phenotypic diversity in Anacamptis coriophora (Orchidaceae) populations from South Romania
Get PDFRomanian flora includes a high diversity of orchids, any with a high conservationist interest, but many 0f themnot yet sufficiently investigated from an ecological, phytosociological or genetic point of view. Phenotypically analysis is also scarce and only a few data have been published in relation to the effects of site factors on the morphological and anatomical characteristics of the family Orchidaceae. This study analyses the variability of two populations of Anacamptis coriophora, based on their coenological affiliations, morphological observations and biometrical measurements. The data were collected from two grassland areas in Romania Stoenesti, Vâlcea County and Cobia, Dâmbovița County. The results showed that both populations are influenced by temperature, light and humidity and develop normally under optimal conditions. In the most favorable conditions encountered in Vâlcea county, orchids offer the splendor of flowers for a long time. Orchids in our country usually bloom in March, sometimes lasting until August, they have a delicate appearance, varied colors and the most diverse perfumes. The information obtained regarding the morphological diversity of the two populations could be suitable for designing strategies for their conservation in both locations
Identification and bioinformatics analysis of MADS-box family genes containing K-box domain in maize
Get PDFThe MADS-box family genes are involved in the development of plant roots, leaves, flowers, and fruits, and play a crucial role in plant growth and development. Studying MADS-box genes with K-box domain is crucial to distinguish different types of MADS-box genes. This study systematically analysed the genomic structural information of maize MADS-box family members containing the K-box Domain at the genome-wide level using the maize (Zea mays) B73 genome as the reference sequence, and provided insight into the biological functions of the maize MADS-box family containing the K-box domain. According to the findings, 52 MADS-box family genes with K-box domain were identified and divided into 4 subgroups. The distribution of motif in the same subgroup was found to be relatively conservative, and all of them had MADS-box conserved domain and K-box domain. Gene structure analysis showed that the introns and exons of the same subgroup genes have similar gene structure, and different types of genes containing the K-box domain showed different exon/intron structure characteristics. Chromosome mapping showed that 52 genes containing the K-box domain were unevenly distributed on the 10 chromosomes of maize, most of which were distributed at both ends of the chromosome and a small number of genes were distributed near the centromere. Based on the analysis of cis-acting elements of it up-stream promoter, it was found that MADS-box family genes may be involved in light response, IAA, GA, ABA, and LTR signal pathways, indicating that they play a certain role in stress response and hormone signal transduction. The expression analysis of genes with the K-box domain in maize leaves treated with auxin and gibberellin revealed that MADS-box genes may have a regulatory effect on certain plant hormones. Through the identification and bioinformatics analysis of MADS-box family genes containing the K-box domain, it is helpful to further study the function and pathway of MADS-box family genes, and provide a theoretical basis for further re-search for the molecular mechanism of maize growth and development