Publications of the Indonesian Scholars' Alliance
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BIOACTIVE ACTIVITY OF A RECOMBINANT LONGAN (Dimocarpus longan LOUR.) SEED PEPTIDE
Get PDFBackground: Consumption of antioxidants has been evident to prevent diseases caused by free radicals damage. Antioxidants can be found in the form of peptide in various natural sources. From our previous study, to overcome obstacles of direct longan seed hydrolysate extraction, the recombinant Longan1 peptide which contains 4 repeats of ISYVVPVYIAEITPKTFRGGF linked by D was produced from Escherichia coli. The in vitro bioactive properties of this recombinant peptide were characterized.
Methods: The recombinant and chemically synthesized Longan1 peptides were tested for bioactive activity including, DPPH, ABTS, and nitric oxide radical scavenging assays, the ability to protect plasmid DNA from hydroxyl radicals, anti-proliferative activity to several cancer cell lines, and antiinflammatory effects in cell culture level.
Results: The recombinant peptide revealed antioxidative activities, including DPPH, ABTS, and nitric oxide radical scavenging activity, which are similar to the chemically-synthesized one. However, the recombinant peptide exhibited higher in vitro ability to protect DNA from hydroxyl radicals. The IC50 value of the recombinant Longan1 peptide could only be calculated through the assay of antiproliferation of stomach KATO-III cancer cell line, while IC50 value from the chemically synthesized peptide could not be calculated in any tested cell lines. Finally, the anti-inflammatory effect determined by the inhibition of nitric oxide production from macrophages RAW 264.7 activated by LPS revealed that the recombinant Longan1 peptide could inhibit nitric oxide production from macrophage cells, whereas the chemically-synthesized one could not.
Conclusion: With all these properties, the recombinant Longan1 peptide seems to have bioactivity that can possibly be a candidate for further medical application or supplementary products.
 
PERCEIVED INSUFFICIENT MILK (PIM) AMONG MOTHERS OF 0-6 MONTHS INFANTS IN CIPAYUNG HEALTH CENTRE, DEPOK INDONESIA: A QUALITATIVE STUDY
Get PDFBackground: Exclusive breastfeeding is breast milk given to infant since their birth to the age of 6 (six) months, without adding and/or replacing with other foods or beverages (except medicines, vitamins, and minerals). One of the factors that affect the exclusive breastfeeding was the perceptions of insufficient milk. Milk insufficiency is considered to happen when the mother stops breastfeeding exclusively with some reasons such as no breast milk, low milk supply, or insufficient milk. This research was to examine more deeply the perception of insufficient milk and factor influencing it.
Methods: This was a qualitative study that used a primary data of study "Intervention of Breastfeeding Enhancement and Weaning Food in the Implementation of the First-1000-Days-of-Life Program". Informants selected through who has been intervened research by PKGK. Triangulation of data sources was a midwife, informants' husbands, documents of mother and child health books (MCH) and secondary data from PKGK research.
Results: Based on the result from the interview: a) there was found a tendency of knowledge increase on mothers receiving treatment. b) Mostly, the lactation guidance was given without supporting device. c) Mother perception about their will gained weight if giving the sufficient milk has not been in line with Body Mass Index (BMI) before pregnancy. d). Family support towards mothers breastfeeding has not been provided optimally. e) Early breast-feeding (EBF) was not complete in accordance with thestandard.
Conclusions: Mothers who had not received treatment were having lack of knowledge on exclusive breastfeeding. Implementation of EBF was not in line with the procedure. The role of a delivery helper on EBF is vital in order to make the implementation of the EBF carried out according to the procedure.
 
COST AND FINANCIAL ECONOMIC IMPACT IN CATHETER ASSOCIATED URINARY TRACT INFECTION (CAUTI): A SYSTEMATIC REVIEW
Get PDFBackground: Catheter-associated urinary tract infections (CAUTI) was the first condition chosen for nonpayment because of its anticipated effect on large numbers of hospitalizations. The risk of CAUTI is mainly related to the duration of catheterization, occurring at a rate of 5% per day. This study is to identify the cost and financial economic impact of intervention and prevention including an indication of CAUTI, number of events, duration of urinary catheterization, length of stay, the cost for antibiotics andhospitalization cost in catheter-associated urinary tract infection (CAUTI) to reducing the rate and hospital payment.
Methods: Systematic Review with PRISMA – P Protocol in 2009 method from a relevant database such as PubMed and ProQuest search engine in 2007 until 2017. Total document selected by full text is 10 article.
Results: Based on literature search and screening article after using intervention and prevention strategy for CAUTI in the USA, UK and Thailand have a good impact in the duration of urinary catheterization, length of stay, the cost for antibiotic and hospitalization cost. Some article result that the change in the rate of CAUTI was not significantly different before and after the policy and validity in identifying CAUTI is limited.
Conclusions: Cost impact in catheter-associated urinary tract infections (CAUTI) can preventable by reducing unnecessary urinary catheter use (appropriate indication), shortening catheter duration, bladder bundles intervention, supervision, and evaluation of the implementation.
 
INTEGRATED REHABILITATION PROGRAM FOR PERSONNEL WITH DISABILITIES IN THE MINISTRY OF DEFENSE AND INDONESIAN NATIONAL ARMED FORCES
Get PDFBackground: In carrying out their main duties, soldiers are often confronted with risks that can cause them to have disabilities either when carrying out operational tasks or having accident in the service. Efforts to improve the life of persons with disabilities are empowered through integrated rehabilitation process for Personnel of The Ministry of Defense of the Republic of Indonesia and Indonesian National Armed Forces with disabilities. This research was aimed to describe and analyzing the integrated rehabilitation on persons with disabilities at the Ministry of Defense Rehabilitation Center, and describing the factors that support and inhibit the implementation of integrated rehabilitation.
Methods: This research was a descriptive qualitative research with an in-depth interview as instrument to obtain data from informants who were the participants of integrated rehabilitation.With Triangulation the source was the instructor and the rehabilitation organizer.
Results: Based on the results of interview analysis conducted, the researchers also have identified several supporting factors and inhibiting factors on the implementation of integrated rehabilitation program. The inhibiting factors are Resources: the limited number of instructors (human resources) that have sufficient competence and the lack of facilities for the accessibility of persons with disabilities this will hinder the implementation of rehabilitation. From the results of research indicates that communication, disposition and bureaucratic structure is a supporting factor in the implementation of integrated rehabilitation.
Conclusions: Implementation of integrated rehabilitation has been running well although there are still inhibiting factors. Therefore, the Ministry of Defense Rehabilitation Center should continue to strive to become professional rehabilitator in order to create self-sufficient and productive persons with disabilities so as to improve their welfare.
 
Selective Abortion After Preimplantation Sex Selection: An Ethical and Legal Issue in Indonesia
Get PDFBackground: The emerging of sex selection technology in Indonesia is sperm sorting, meanwhile sex selection with Preimplantation Genetic Diagnosis (PGD) methods is not widely used. The use of sperm sorting has bigger chance to fail than PGD, thus potentially cause ethical and legal problems that is selective abortion during pregnancy. The potency for selective abortion is enlarged by Indonesian law that permitting sex selection without distinction of medical and non-medical reasons. There is no special policy to regulate the selective abortion because of sex selection failure. Aims: This study aims to find out the legal concept of selective abortion after preimplantation sex selection that appropriate to be applied in Indonesia.Methods: This research is normative research that use analytics method with legal approach and conceptual approach. The research material consists of primary legal material (legislation about sex selection and abortion in Indonesia), secondary legal materials (legal journals, law books, and legal proceedings), and also non-legal materials (journals, books, and health proceedings about sex selection and abortion).Results: In Indonesia meanwhile in general, abortion is permitted for pregnancy with medical indication and rape victim. Through a statue approach in Indonesia, selective abortion after preimplantation sex selection can be implemented for strong medical reasons. While the failure for non-medical reasons can't be aborted. This regulation contrary with ethics, morals and religion. Selective abortion should not be done because of preimplantation sex selection failure either medical or non-medical reasons. Conclusion: Selective abortion after preimplantation sex selection both medical and non medical reason contrary with moral, ethical, and religion. Indonesia needs to regulate further policy about selective abortion if there is a failure of preimplantation sex selection
Factors Influencing Stigma to Lesbian, Gay, Bisexual, and Transgender (LGBT) Among Teenagers at Ngaran Village, Gamping, Sleman, Indonesia
Get PDFBackground: As the part of sexual orientation varieties and gender identities, until now the existence of Lesbian, Gay, Bisexual, and Transgender (LGBT) still becomes a social, theological, and psychological problem. Similar to trauma, low self acceptance, low self esteem, and anxiety, if it is not handled properly, the condition will be getting worse.
Aims: The aim of the study is to increase prevention efforts toward growing phenomena of TGBT on teenagers.
Methods: This is a quantitative study with one group pretest and posttest design. The work involved a total of 41 teenagers at at Ngaran Village, Balecatur, Gamping, Sleman, Indonesia. A set of questionnaires was given to the respondents to measure knowledge, attitude, perception, service access, and also peer's attitude to LGBT. Counseling was given once together with material content about LGBT awareness in family. Statistical analysis was employed to process and analyze the data.
Results: The result showed factors correlated to stigma about LGBT on teenagers namely knowledge, attitude, perception, access to any service, and peer's attitude. The most influential factor was knowledge factor with p value 0.00 and peer's attitude with p value 0.02.
Conclusion: After being participated in the study, it is expected that teenagers can give information to the family members, so the family members and the society will have appropriate knowledge and attitude related LGBT stigma.
Received: 6 February 2018, Reviewed: 12 February 2018, Received in revised form: 16 June 2018, Accepted: 27 June 2018
POTENTIAL ACCELERATING EFFECT OF Ageratum conyzoides L. LEAVES EXTRACT ON FIBROBLASTS DENSITY OF INCISION WOUND OF MALE WHITE MICE (Mus musculus)
Get PDFBackground: Wound treatment using traditional medicine has been known widely in various countries in the world. Ageratum conyzoidesL. is commonly known by the ancient people to treat wound due to its potential anti-inflammatory effect. This study aims determine the effect of Ageratum conyzoidesL. leaves extract on fibroblast density of incision wound of male white mice (Mus musculus).
Methods: This post-test only control group design experimental study used 35 male white mice which were randomly divided into five groups, i.e. negative control group K(-), positive control group K(+) (10% povidone iodine), and treatment group P1, P2, and P3 that were each given billy-goat weed leaf with increasing dose (15%, 30%, and 45% respectively). On each day, the length of the incision was measured by a ruler. After 7 days, the mice were terminated to obtain wound tissue which were used to prepare H&E stained histopathological sections to observe fibroblast density. Non-parametric analyses using Kruskall-Wallis and Mann Whitney test were used to compare the wound length and fibroblasts density.
Results: Lengths of incision wound between all pairs of groups at the 7th day are significantly different (p< 0.05) with group P3 showed the shortest one. Significant differences were also observed in fibroblasts density between group K(-) and K(+), K(-) and P1, K(-) and P2, K(-) and P3, K(+) and P3, P1 and P3, P2 and P3 (p< 0.05) with group P3 showed the highest density among all groups.
Conclusions: Ageratum conyzoidesL. leaves extract 45% has more potential effect than povidone iodine 10% in accelerating healing process by enhancing fibroblasts density.
 
FACTORS ASSOCIATED WITH BIRTH SPACING AMONG CHILD MARRIAGE COUPLES IN INDONESIA
Get PDFBackground: Child marriage has been proved resulting adverse outcomes for both groom and brides. Those who married before the age of 15, are more likely to have more than 3 children before they are 24 years old. This, along with the fact that girls are not physically mature enough to give birth, places both mothers and their babies at risk. This study aims to review factors that might influence decision making on spacing birth among child marriage couple in Indonesia.
Methods: Data from Riset Kesehatan Dasar (Basic Health Research) of Indonesia 2013 were employed with additional several papers from other countries to strengthen the analysis. The theory of Planned Behavior (TPB) were used to explain that behavior is not fully under control of the individuals.
Results: Girls aged of 15-19 were less likely to use contraception. Study shows the birth spacing was affected by attitudes toward behavior. The other factors are subjective norms, where people surrounding are influencing the birth spacing decision. Internal factor such as the affordability of the service may influence them to access the services. External factor such as the availability of the services will help them easier in accessing contraceptive for birth spacing.
Conclusions: Child marriage trends are start to decline year by year. Yet, the impact of child marriage needs to be reduced to make a better quality of life of the child marriage. A good practice and positive attitudes to birth spacing will lower the adverse impacts to the brides, and it can give a better outcome for the children as well.
 
The effectiveness of discharge planning and range of motion (ROM) training in increasing muscle strength of nonhemorrhagic stroke patients
Get PDFBackground: Non-hemorrhagic stroke patients experience hemiparesis, an improper handling results in joint contractures. Discharge planning combined with a range of motion (ROM) training given to patients and their families are expected to improve muscle strength in patients after returning from the hospital.Aims: This study is to identify the effectiveness of discharge planning in increasing muscle strength.Methods: This is a quasi-experimental study with a pre-posttest design. A total of 34 respondents were selected by cluster random sampling technique, from RAA Soewondo Pati General Hospital of Pati, Central Java, Indonesia. The respondents were divided equally into two groups; an intervention group (N = 17) was given a discharge planning program together with stroke information and range of motion (ROM) training while the control group (N = 17) received a standard discharge planning available in the hospital. Further, Muscle Rating Scale (MRS) was employed to assess the muscle strength on the 2nd, 7th, and 14th day after discharge planning presented to the nonhemorrhagic stroke patients.Results: This present study clearly acknowledges the standard discharge planning program available in the hospital improve the muscle strength of the upper and lower extremity in the nonhemorrhagic stroke patients just 2nd day after the care (pretest), and the significant improvement was observed until the day 14. Moreover, combining the care with ROM training at the intervention group faster the recovery and the muscle strength improved significantly at the 7th day and continue increase at the day 14. Looking to the muscle strength since the 2nd day to the day 14, respectively the muscle strength of upper and lower limb at the control group improved at the point of 0.588 and 0.882, while at the group received the ROM training reached the value of 1.472 and 1.412.Conclusions: The ROM training combined to the current discharge planning program will faster the muscle strength recovery of the nonhemorrhagic stroke patients. This research provide insight how family plays important role to the success in monitoring the rehabilitation and recovery progress.
Keywords: Discharge planning, Range of motion (ROM) training, Nonhemorrhagic stroke patients, Muscle strength
Received: 25 August 2018, Reviewed: 01 September 2018, Received: in revised form 23 October 2018, Accepted: 31 October 2018
DOI: 10.35898/ghmj-2355
Stress, coping, and mental health status among nursing students at a private university in Nakhon Pathom, Thailand
Get PDFBackground: Stress, especially among young people, leads to life-threatening mental conditions such as depression and suicide. Nursing students, in particular, are exposed to different kinds of stress such as pressures from both academic and clinical exposures coupled with expectations to succeed. These stressors influence individual coping styles which may eventually affect students' mental, physical, and over-all wellbeing leading to the decline in their learning and academic performance.Aims: This descriptive-correlational study was aimed at exploring the relationships among stress, coping, and mental health status among nursing students at a private university in Nakhon Pathom, Thailand.Methods: Using a systematic random sampling, a descriptive cross-sectional study was done among one hundred and fifty 3rd and 4th year nursing students under the international nursing program. The Perceived Stress Scale, Brief COPE, and the General Health Questionnaire were used to assess the level of stress, the coping strategies used, and the mental health status of the respondents. Descriptive statistics, t-test, and Pearson's correlation were used to answer the research questions.Results: It revealed that the nursing students had moderate level of perceived stress, used acceptance as the most common form of coping, and substance use and denial as the least used. Furthermore, the respondents had mild level of mental health related illness. Significant gender differences were found in the perceived level of stress, and use of coping strategies. While mental health status significantly differed according to year level and interest in nursing. Significant mild to moderate relationships were found among perceived stress level, coping strategies, mental health status, and selected socio-demographic variables.Conclusion: The findings of the study provide additional useful information on the relationships of stress, coping, and health outcomes. Results can also be useful in creating a stress management program for nursing students such as awareness on individual stress response and reinforcing the use of healthy coping strategies.
Keywords: Stress, Coping, Mental Health, Nursing students, Thailand
Received: 08 August 2018, Reviewed: 10 September 2018, Received: in revised form 17 October 2018, Accepted: 21 October 2018
DOI: 10.35898/ghmj-2355