Journal of Experimental Biology and Agricultural Sciences
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The Effect of Titanium Dioxide Nanoparticles on Haematococcus pluvialis Biomass Concentration
Get PDFThe increased release of Titanium dioxide nanoparticles (TiO2 NPs) into the aquatic ecosystem is caused by the augmented utilization of nanoparticles in personal care and household products. This has resulted in the contamination of marine, aquatic, and ground water resources, causing adverse impacts on the biota and flora, both in vivo and in vitro. The main purpose of this research was to examine the negative impacts of TiO2 NPs on the bioaccumulation of Haematococcus pluvialis. The interaction and buildup of TiO2 NPs on H. pluvialis were studied using scanning electron microscopy (SEM). The exposure of H. pluvialis to TiO2 NPs with increasing concentrations (5–100 μg/mL) and time intervals (24 h to 96 h) impacted the biomass concentration of the microalgae. The SEM images provided evidence of changes in characteristics and impairment of the exterior of exposed cells. The findings revealed that the exposure of H. pluvialis to TiO2 NPs resulted in a decline in biomass, which was dependent on the concentration and duration of exposure. The most severe adverse effects were observed after 96 hours of exposure, with a reduction of 43.29 ± 2.02% of biomass concentration. This study has demonstrated that TiO2 NPs harm H. pluvialis, as evidenced by the negative impact on algal biomass resulting from the binding and buildup of these particles on microalga H. pluvialis. To sum up, the decline in algal growth is caused by the accumulation and interaction of TiO2 NPs on microalgae scoring the adverse effects on the growth of H. pluvialis by TiO2 NPs. The findings of this study call for novel screening methods to detect and eliminate TiO2 NPs contamination in aquatic sources used for the cultivation of microalgae which may otherwise pose delirious effects to the consumers
Prospective nutritional, therapeutic, and dietary benefits of camel milk making it a viable option for human consumption: Current state of scientific knowledge
Get PDFFor over five thousand years, people in Asia and Africa have known about the health benefits of camel milk. Thus, it is used not only as a food source but also as a medicine. The similarities between camel milk and human milk have been scientifically proven. Camel milk is unique among ruminant milk because it is high in vitamins C and E and low in sugar and cholesterol. Still, it contains a wide variety of beneficial minerals (including sodium, potassium, iron, copper, zinc, and magnesium), besides being rich in several nutrients, including monounsaturated and polyunsaturated fatty acids, serum albumin, lactoferrin, immunoglobulins, lysozyme and the hormone insulin. Because of these components, many medical professionals now recommend camel milk as a treatment for various human ailments. It has been demonstrated to be effective in treating gastrointestinal issues, Type 1 diabetes, and food allergies. As a bonus, camel milk has been utilized to cure autism, lower cholesterol, prevent psoriasis, heal inflammation, aid tuberculosis patients, boost the body's natural defences, and impede the spread of cancer cells. Those who have problems digesting lactose may still be able to tolerate it. Conversely, camel milk can also help reduce an excessively high bilirubin, globulin, and granulocyte count. Drinking camel milk does not affect the erythrocyte sedimentation rate, hemoglobin concentration, and leukocyte count. The proteins in camel milk have an adequate ratio of critical amino acids. Immunoglobulins, which fight disease, are contained inside, and their small size allows antigens to penetrate and boosts the immune system's efficacy. This article highlights the health benefits and medicinal uses of camel milk
Immune-Cell-Mediated Cancer Treatment: Advantages, Drawbacks And Future Direction
Get PDFCancer ranks as the most lethal and prevalent non-communicable disease in clinical settings. Therapeutic options for cancer comprise chemotherapy, radiotherapy, surgery, and combined treatment. Cancer remission and relapse cases are widespread despite having various advanced medications and sophisticated dissection techniques. A new approach involving immune-cell-mediated cancer therapy has been adopted extensively for cancer treatments by utilizing immune cells. Immunotherapy has gained much attention to prevent and treat various types of cancer. Immunotherapy treatments operate in multiple contexts. Several immunotherapy therapeutic interventions assist the immune function in halting or reducing the advancement of cancer cells. Many also facilitate the immune cells in destroying cancerous cells or safeguarding against cancer from disseminating to certain other regions of the human body. Among other methods, genetic manipulation of immune cells offers hope for innovative anticancer treatment. T lymphocytes and natural killer cells have become the most extensively documented immune cells for immunotherapy. Chimeric antigen receptor T-cell therapy exhibits the most promising blood cancer treatment. However, adoptive NK cell transfer therapy displays potential anticancer treatment options, although more research is needed to be carried out. In addition, cytokine-induced immunomodulation is also plausible for cancer immunotherapy. This review will highlight the most comprehensive information, observations, and consequences associated with different cancer immunotherapy initiatives
Productivity and profitability of commercial broiler chickens under various farming conditions
Get PDFBroiler farming plays a vital role in fulfilling global protein requirements. Although broiler farming is considered profitable, profitability might be affected by factors such as genetics, feed quality, and management practices. In the current study, the productivity and profitability of commercial broiler farming were studied under various farming conditions, such as farm size, location of the farm, and mortality of the broilers. Data were collected through farmers' interviews and farm record books and processed and analyzed to determine the productivity and profitability of broiler farming. Productivity and profitability did not differ significantly across farm sizes and locations. The results of the study reported mortality as a factor affecting productivity and profitability in broiler farming. It was manifest that mortality adversely affected the productivity and profitability of broiler farming. A significant positive relationship was recorded between mortality and feed conversion ratio. Moreover, mortality was negatively correlated with the gross margin of broiler farming, meaning that the low gross margin was due to the high mortality at broiler farms. The farms were more profitable when the mortality was <5%, compared to >10%. It is recommended to reduce the mortality percentage of broiler chickens as minimum as possible, preferably <5%. Good quality chicks, better management, and the prevention of diseases might play an important role in keeping the mortality rate at a minimum level in broiler farming
Colistin the last resort drug in 21st century antibiotics to combat Multidrug resistance superbugs
Get PDFPolymyxin' E' (Colistin) is considered the last resort therapy against Multidrug resistance (MDR) bacteria, mainly Klebsiella peumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, and Escherichia coli and play a critical role in causing life-threatening infection, and their prevalence is increasing as a big concern globally. Apart from immunological adaptation, chromosomal mutations and plasmid-mediated genes are mostly associated with this resistance at the molecular level. Therefore, the current review extensively focused on Colistin as a drug in 21st-century antibiotics, the activities spectrum with diverse resistance mechanisms of bacteria against Colistin, and emerging approaches of Colistin from discovery to tackling MDR. In the study, we got to know about the challenges and new developments with old weapons like phage therapy as well as new approaches like Phage display and drug repurposing, in addition to the chromosomal and plasmid-mediated genes that play a role in antimicrobial resistance (AMR). The present study would provide insight into the prognostic aspect of combating MDR
The Impact of Elicitation on Potato (Solanum tuberosum L.) Production, Enzymatic and Antioxidant Activity in Nuevo León, Mexico
Get PDFMany compounds available in the market act as elicitors and can be incorporated into agronomic management. But the focus is on elicitors frequently used for the induction of different responses related to the systemic resistance of plants to increase the production of bioactive metabolites, biomass accumulation, and yield. For that case, this work aimed to evaluate the effects of three elicitors on potato crops under field conditions. The potato cultivar "Fianna" was used, and a completely randomized design with four treatments and four repetitions. The effect of three elicitors at a dose of 2.5 g. L-1 for Activane®, 2.5 ml. L-1 for Micobiol® and 2.5 g. L-1 for Stemicol® was evaluated on growth, yield, enzymatic and antioxidant activity. Generally, the elicitors had a positive effect on the enzymes and antioxidant capacity of the potato plant. It was concluded that the application of elicitors Stemicol® (T4) had the most significant result on the number of tubers and weight per plant at harvest while allowing a more substantial number of tubers to be obtained. In comparison, Activane® (T2) influenced the growth variables of stem length and number of leaves per plant
Isolation and characterization of chlorpyrifos-degrading bacteria in tea-growing soils
Get PDFThe excess use of pesticides in the agricultural sector has caused environmental pollution and affected the complete ecosystem. Among the various commonly used pesticides, chlorpyrifos (CPF) is widely used against multiple agrarian pests due to its effectiveness and higher insecticidal activities. However, along with its beneficial usage, CPF has various residual effects on the environment, causing multiple negative impacts on aquatic organisms and human health. Consequently, methods for eliminating CPF in the background are essential. Among the currently available approaches to CPF remediation, biological methods using microorganisms are eco-friendly and cost-effective. Therefore, this study was conducted to isolate and characterize chlorpyrifos-degrading bacteria from the tea-growing soil of Vietnam. For this, soil samples were collected from the 20 tea-growing areas of Vietnam. From the collected samples, three bacterial strains viz., Methylobacterium populi CNN2, Ensifer adhaerens VNN3, and Acinetobacter pittii CNN4 have been isolated by using streak plate method and identified based on 16S rRNA gene analysis. The study results showed that under laboratory conditions, E. adhaerens VNN3 had the highest CPF degradation ability and was followed by the strain M. populi CNN2. In liquid medium, CPF concentration (100 mg/L) was reduced by 95.2% and 81.4% by E.adhaerens VNN3 and M. populi CNN2, respectively, after 72 h. Further, under in-vitro conditions, the concentration of CPF was reduced from 500 mg/kg to 112 ± 1.73 (77.6%) and 197 ± 2.08 mg/kg (60.6%) by E. adhaerens VNN3 and M. populi CNN2, respectively. Based on the obtained results, it can be concluded that E. adhaerens VNN3 and M. populi CNN2 can be used for CPF-contaminated agricultural soil remediation
Isolation and production of polyhydroxybutyrate (PHB) from Bacillus pumilus NMG5 strain for bioplastic production and treatment of wastewater from paper factories
Get PDFPolyhydroxybutyrate (PHB) has the potential to replace traditional plastics and limit environmental pollution caused by plastic waste. This study combined wastewater treatment with PHB production to reduce costs. Bacteria capable of synthesizing PHB were isolated from paper mill wastewater and identified using Matrix Assisted Laser Desorption/Ionization–Time of Flight (MALDI-TOF) mass spectrometry and 16S rRNA gene analysis. Bacillus pumilus NMG5 strain was found to have a good yield in modified Nutrient Broth culture, reaching 42.28% of dry biomass. The PHB product was analyzed using FTIR spectroscopy and 1H NMR spectroscopy. The bacterial strain was also tested for its ability to treat paper mill wastewater, and it showed impressive results in terms of biochemical oxygen demand (COD), total nitrogen, and total phosphorus, with efficiencies of 95.93%, 79.36%, and 83.55%, respectively. The study found that wastewater treatment combined with PHB production was a promising solution to reduce costs and limit environmental pollution. The bacterial strain B. pumilus NMG5 had a high yield of PHB, and the PHB product was of high quality, as confirmed by FTIR and 1H NMR spectroscopy. Furthermore, the bacterial strain showed impressive results in treating paper mill wastewater with high COD, total nitrogen, and total phosphorus efficiencies. These results suggest that this harmless bacterium could be used in paper mill wastewater treatment systems to produce PHB, providing a sustainable and environmentally friendly solution
Usage of iron foliar spray in enhancing the growth and yield of the flax plant (Linum usitatissimum L)
Get PDFThe ideal growth and development of linseed plants depend on receiving the necessary nutrients during the growing season when they are grown. Flax's yield and oil content increase using a foliar spray containing micronutrients. This study aimed to determine how foliar iron (Fe) treatment affected flax yield and its constituents. The experiment was set up at the adoptive research farm Sargodha in a randomized block design and three replicates. At the capsule filling stages and bud initiation of the flax crop, foliar sprays with varying concentrations of Fe (5.5%, 4.5%, 3.5%, 2.5%, 1.5%) and without Fe (control) were administered. Sulphate of iron (Fe) was used as the source of Fe. All treatments resulted in notable enhancements in agronomic characteristics such as grain oil contents, harvest index, biological yield, number of capsule formations, technical stem length, plant height, as well as physiological parameters including fluorescence yield (Ft), quantum yield (YII), photosynthetically active radiation (PAR), electron transport rate (ETR), and chlorophyll contents. The results of this study suggested that the application of 3.5% to flax during the bud initiation and capsule filling stages increases the seed yield, yield attributes, and oil contents. In conclusion, foliar spray of Fe could enhance the yield of linseed crops
Effect of nutrient management on physio morphological and yield attributes of field pea (Pisum sativum L.)
Get PDFA field experiment was conducted to investigate the impact of nitrogen management on the growth and yield of field peas. The experiment took place during the rabi season (October–March of 2022–2023) at Lovely Professional University's Agriculture Research Farm in Phagwara, Punjab, India. Fifteen different treatment combinations were utilized, involving the application of chemical fertilizers (NPK) and micronutrients (boron and zinc). The experimental design followed a randomized complete block approach with three replications. Among the treatment combinations, the application of foliar spray with B at a rate of 0.2%, Zn at a rate of 0.5%, along with 100% recommended dose of fertilizer (RDF), resulted in the highest measurements for plant height (70.44 cm), leaf count (70.60), branch count (18.86), leaf area (32.24 cm²), dry matter accumulation (6.12 g), crop growth rate (0.299 g m⁻² day⁻¹), and relative growth rate (0.05933 g g⁻¹ day⁻¹). Furthermore, treatments involving 100% RDF, 0.2% B, and 0.5% Zn exhibited enhanced yield characteristics, including the number of seeds per pod (10.26), pods per plant (12.33), test weight of seeds (15.06 g), seed yield (3537 kg ha⁻¹), and harvest index (47.49%). Furthermore, 100% RDF and the inclusion of 0.2% B and 0.5% Zn outperformed the control. Applying 100% RDF along with the micronutrients B and Zn is recommended to maximize production and net profit in field pea cultivation