Journal of Agriculture and Applied Biology
Not a member yet
125 research outputs found
Sort by
Development of Climate Requirements for Compatibility of Land Cocoa in Polewali Mandar District
No full textCocoa as leading plantation commodities which is quite essential for the national economy that decreasing productivity from 2012 to 2018. The low productivity of cocoa is caused by the fact that cocoa plants that are planted do not meet the optimum growth requirements. Land quality as an optimal growth factor varies greatly in land and time quality, so it requires an in-depth study, one of which is the parametric approach. This approach ana-lyzes the land requirements climate for the suitability of specific cocoa plan-tations in particular regions, so this study aims to determine the characte-ristics of the land that correlate with cocoa productivity in a specific region. The study was conducted from July to December 2019. Also, this study aims to establish a land index through a deductive parametric approach in the Polewali Mandar district as a sample of research locations. The Research purpose of this study was to determine the land characteristics climate) in Polewali Mandar District. This study used a qualitative-quantitative mix analysis. Quantitative analysis is used in determining the correlation be-tween land characteristics climate and cacao production in each sub-district. Determination of land characteristics climate that has a significant effect using regression analysis Primary data were obtained from direct observation, while secondary data were obtained from related institutions. Besides, the determination of the profile location based on production data and cocoa crop distribution through satellite imagery. Qualitative and quan-titative analysis using regression data analysis was used. The results showed that the climate and soil conditions of the Polewali Mandar District were very suitable for the level of land suitability. All parameters tested in this study illustrate that land characteristics climate in the Polewali Mandar District is very suitable for the optimum growth requirements of cocoa plants
Growth and yield of maize (Zea mays L.) as influenced by date of sowing and hybrids
No full textA field experiment was conducted at college of Agriculture, UAHS, Shivamogga during kharif 2015 to study the effect of date of sowing and hybrids on growth and yield of Maize (Zea mays L.). The experi-ment was laid out in randomized complete block design (RCBD) with factorial concept and replicated thrice. There were eight treatment combinations which includes four dates of sowing (15th June, 30th June, 15th July and 30th July) and two hybrids (PAC-740 and CP-818). Crop sown on 15th June recorded significantly higher plant height (201.03 cm), number of green leaves (3.03), leaf area (992.49 cm2), LAI (0.74), total dry matter (305.65 g), cob length (22.16 cm), kernels cob-1 (670.93), kernel yield cob-1 (230.95 g), test weight (43.08 g), kernel yield (7632.57 kg ha-1), stover yield (9512.56 kg ha-1) and har-vest index (44.52 %) as compared to other sowing dates. Among the hybrids CP -818 recorded significantly higher plant height (191.85 cm), number of green leaves (2.72), leaf area (954.32 cm2), LAI (0.71), total dry matter (277.65 g), cob length (19.81 cm), kernels cob-1 (541.88), kernel yield cob-1 (207.71 g), test weight (39.16 g), kernel yield (7060.72 kg ha-1), Stover yield (8839.98 kg ha-1) and harvest in-dex (44.44%) as compared to PAC-740. The interaction between dates of sowing and hybrids are non-significant
Antioxidant Activity of Endophytic Bacterial Extract Isolated from Clove Leaf (Syzygium aromaticum L.)
No full textClove (Syzygium aromaticum L.) is one type of spice plant that has high antioxidant content because of the high eugenol compounds. However, iso-lating these bioactive compounds require a significant amount of biomass. A known alternative solution to overcome the problems is through the utiliza-tion of endophytic bacteria in clove leaves. Endophytic cloves leaf bacteria can be a good antioxidant resource. Endophytic bacteria are bacteria that grow in plant tissues that is not pathogenic, which can replace these plants. The aim of this study is isolating endophytic bacterial from clove leaves, screening endophytic bacteria, determining potential endophytic bacteria as producer antioxidant compounds, and identifying potential isolate using 16S rRNA. This study used endophytic bacteria isolated from clove leaves (Syzygium aromaticum L.). Antioxidant activity determined using DPPH method. It was founded 7 isolates of endophytic bacteria namely WDY1, WDY2, WDY3, WDY4, WDY5, WDY6, and WDY7. The isolate WDY6 shows the highest antioxidant activity. WDY6 isolate is identical to Staphylococcus sp. with a 100% similarity. Staphylococcus sp. has the highest antioxidant activ-ity in the stationary phase. The content of chemical compounds from WDY6 isolates is pyrazine that belongs to alkaloids
Determination of Equilibrium Moisture Content in Trade Distribution
No full textMaize is one of essential crop in Indonesia. Maize trade distribution showed economic condition in society. If the distribution trade was poor, the quality and quantity of product will be low, consequences to uncontrolled condition in society. Every actor in distribution trade play important role. Farmer for instance, if the farmer produces low quality of maize grain, will resulted unacceptable product in factory. The acceptance of maize in feed factory is determined by several factor, such as moisture content, microorganism contamination, and presence of pest. To know the best recommendation treatment to prevent rejected product in feed factory, it is important to de-termine the moisture content in trade distribution, because the higher mois-ture content would increases the possibility of microorganism contamina-tion. Besides, the best condition storage is also critical for keeping the quali-ty of maize grain. Therefore, determination of equilibrium moisture content (EMC) will help the policies maker to consider environment condition for their storage. In this study, the maize EMC was determined using Hender-son’s equation. The results showed that the highest EMC was found in the maize after threshing (23.49±0.58%). The postharvest treatment, such as drying and sortation is very important apply in such poin
Investigating the anti-acne potential of endophytic bacterial extracts isolated from Mangifera casturi in indigenous South Borneo, Indonesia
No full textEndophytic bacteria are beneficial microorganisms living in the tis-sues system of various parts of a plant, including fruits, leaves, twigs, and roots. The bacteria are stated as forming colonies without caus-ing any damage to the plant. Thus, this study aimed at isolating endo-phytic bacterial from the leaves, barks, and fruits of Kasturi plant (Mangifera casturi), screening its endophytic bacteria, determining the potential of those endophytic bacteria, identifying 16S rRNA and analyzing potential growth of the bacteria. The isolated endophytic bacteria appeared to show potential activity against pathogenic bac-teria Propionibacterium acnes with disc-diffusion methods. Besides, the observations on bacterial activities showed isolate L2, S2 and F4 isolated from leaves, bark and fruits, respectively, as the most potent producers of antibacterial compounds. Technically, those activities were indicated by the formation of clear zones. Molecular identifica-tion was investigated by applying PCR amplification on 16S rRNA gene. Furthermore, the isolate L2 was identified as Enterobacter clo-acae with 99% sequence similarities; however, isolates S2 and F4 were identified as Escherichia coli. Therefore, these findings sug-gested that the identified strains would contribute to any further searches for new sources of anti-acne substances
Anti-inflammatory potential of african leaf water extract
No full textInflammation is the immune system\u27s main response to infection and irritation. African leaf (Gymnanthemum amygdalinum) is one of the medicinal plants that can be used as anti-inflammatory because of the presence of flavonoid compounds. Flavonoid compounds have an anti-inflammatory effect that can regulate arachidonic acid metabo-lism by inhibiting cyclooxygenase (COX) and lipooksigenase activi-ties. This study aims to obtain scientific information and the potential of African leaf extract as an anti-inflammatory in vitro and in vivo test. In this study, in vitro and in vivo anti-inflammatory activity tests were carried out. The in vitro anti-inflammatory activity was evaluated by the ability of African leaf extracts to prevent protein denaturation and in vivo anti-inflammatory activity by observing the reduction of edema in the soles of rats induced by carrageenan by giving three lev-els of doses of African leaf extract. The results showed that the African leaf extract at a concentration of 8273.91 mg L-1 was able to inhibit 50% denatured protein (IC50), while in the in vivo test African leaf had the potential as an anti-inflammatory with the most effective percent-age of inhibition at a dose of 200 mg kg-1 which is 85.20%. African leaf have the potential of approximately 2 times greater than the positive control of diclofenac sodium which is 45.70%
Use of non-chemical methods for the management of southern blight disease of carrot incited by Sclerotium rolfsii
No full textThis experiment was conducted to assess the effect of Trichoderma harzianum, soil solarization, and biofumigant on southern blight dis-ease of carrot incited by Sclerotium rolfsii isolate CS 5. A series of in-vitro and in-vivo trials laid out to select a virulent isolate of S. rolfsii against the carrot variety New Kuroda and evaluated the mustard, cabbage, cauliflower, and broccoli leaf extracts for choosing an effec-tive biofumigant against test pathogen. Mustard was the most effec-tive in inhibiting the radial growth, and sclerotia formation (80.37 and 83.37%) of S. rolfsii (isolate CS 5) at 40% level of concentration followed by cabbage leaf extract (62.22 and 68.69%). On the contrary, a total of 10 isolates of T. harzianum were screened against the test pathogen on Potato Dextrose Agar (PDA) medium for choosing a dominant isolate of T. harzianum. The isolate Th-6 was found as the most active in inhibiting the radial growth (84.44%) of S. rolfsii fol-lowed by Th-1 (75.56%). In the application of T. harzianum, biofumi-gation, and soil solarization, the treatment appeared to be the most superior in reducing pre- and post-emergence mortality of carrot during secondary field trials. The lowest southern blight disease inci-dence (10.77%), and disease severity (12.78%) were found at the same treatment. Subsequently, the yield of carrot was increased 155.18% which might be due to the reduction of carrot disease as well as the addition of organic materials in the soil
Effect of silver nanoparticles, synthesized from fleshy stem and leaves extract of Brassica campestris L., on Alternari lini inhibition
No full textSeveral fungal pathogens showed resistance against pesticides, plant mediated synthesized silver nanoparticles have been recognized as effective against them. The present research work was an attempt to synthesize silver nanoparticles by using fleshy stem and leaves ex-tract of Brassica compestris L. from silver nitrate, which reduces it to synthesized silver nanoparticles. Biosynthesized AgNP’s were char-acterized by different techniques like UV-visible spectrophotometry and Field Emission Scanning Electron Microscopy and fungal inhibi-tory activity by well diffusion method. The colour change of solution indicated the synthesis of silver nanoparticles. UV-visible spectro-photometer showed the peak between 415 to 455 nm for fleshy stem and leaves extract of B. compestris and Field Emission Scanning Elec-tron Microscope analysis revealed that spherical shaped nanoparti-cles with size 20-65 nm. These synthesized nanoparticles showed in-hibitory activity against fungi, Alternaria lini, a linseed blight patho-gen which causes linseed blight disease in Linum usitatissimum L. The 30 μl concentrations of silver nanoparticles had showed significant inhibition to growth of A. lini. These findings may suggest AgNP’s syn-thesized by B. compestris used against plant pathogenic fungi
In vitro assessment of protease production and stress tolerance of mutant isolates of Trichoderma sp.
No full textSoil-borne plant pathogenic fungi cause serious losses in agricultural products. The antagonistic fungi for the control of plant diseases have increased efficiency and use of space has emerged as an alternative to other methods for the protection of agricultural products. One of the fungi used for this purpose is Trichoderma species. In this study mu-tant isolates of Trichoderma spp. were used. The resistance and pro-tease enzyme activities of mutant isolates against abiotic factors such as temperature, drought and salinity were investigated. Mutant iso-lates of Trichoderma sp. were showed differed in tolerance to differ-ent abiotic stress factors. Protease enzyme activity produced by iso-lates was influenced by the tested abiotic factors. In the medium con-taining 30% PEG, the highest protease activity was determined in Tm13 isolate. Indigenous Trichoderma strains produced proteases in high temperature, drought and saline conditions. This indicates that isolates may be promising candidates in agricultural production