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Synthetic trisaccharides reveal discriminating ability of endo-glycosidic linkages by mannosidases in the endoplasmic reticulum
Get PDFGlycans on the proteins in the endoplasmic reticulum (ER) act as signals of glycoprotein folding, secretion and degradation. Especially, secretion or degradation signals are produced by the action of ER mannosidases towards each branch of a tri-antennary (A-, B- and C-branch) Man9GlcNAc2 (M9) glycan. Thus, the resulting product after trimming reaction of M9 serves as different signals for determination of glycoprotein fate. Each branch of all terminal glycosidic linkages of M9 are α-1,2 type, while the adjacent inner glycosidic linkages are different. In this study, we examined whether ER mannosidases showed branch specificity by discriminating between different inner glycosides. For evaluation of the hypothesis, we synthesized four trisaccharides with different glycosidic linkages and applied to mannose trimming assays in the ER fraction derived from mouse liver. Through these assays, we report first experimental demonstration that α-1,2-mannosidases in the endoplasmic reticulum (ER) can discriminate inner glycosidic linkages of synthetic trisaccharides substrates.departmental bulletin pape
Economic Analysis of Regional Contractors based on Survey and Financial Statements : Effect of Qualification Scheme on Human Developments
Get PDF少子化と高齢化で，日本の労働力人口や急速に減少し，技術継承が困難になりつつある。なかでも建設業の人手不足は深刻で，建設コストは上昇し，災害対応やインフラの維持管理にも影響が出てきている。建設業には，法律で定められた技術者と技能者の資格が存在し，公共工事の入札などで技術者のレベルと経験が加点されるしくみがあり，これらに基づいた外国人労働者受入制度も進んでいる。技能者・技術者と資格が与える企業そして労働者個人への影響を，ミクロデータとアンケート調査から検証した結果，資格制度の個人評価は職場の満足度を引上げるが，OJTは満足度を引き下げていることが明らかとなり，団体研修を活用した資格取得の支援と取得後の待遇の改善が求められる。departmental bulletin pape
Repatriates’ Knowledge Transferring Behavior in Multinational Companies : a Comparison with Non-repatriates, Focusing on Motivation, Job Characteristics and Perceived Human Resource Management
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Asura's Three Faces? : Nation State, Multicultural State and Sovereign State in Japan (1)
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