Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)
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Increase in Neutrophil Lymphocyte Ratio in COVID-19 Patients Based on Symptom Severity
Full text linkThe neutrophil lymphocyte ratio (NLR) is an indicator of the presence of a systemic inflammatory response. The neutrophil lymphocyte ratio parameter was calculated by comparing the number of neutrophils and the number of lymphocytes. Patients with severe COVID-19 who did not survive were found to have a higher neutrophil lymphocyte ratio compared to patients with a mild prognosis. Thisstudy uses a descriptive method, which aims to describe the value of the neutrophil lymphocyte ratio in COVID-19 patients based on the severity of symptoms. The average value of high neutrophil lymphocyte ratio (NLR) in COVID-19 patients occurred in the severe (6.12%) and critical (10.78%) categories. The average value of high neutrophils (Neutrophilia) in COVID-19 patients occurred in the severe (76%) and critical (82%) categories. The average value of low lymphocytes (lymphocytopenia) in COVID-19 patients occurred in the severe 17% and critical 12% category. The NLR value in patients with the severe category had an increase in the NLR value twice thenormal value, while in the critical category had an increase in the NLR value three times the normal value.
Potential of Hibiscus (Hibiscus rosa-sinensis L.) Extract as a Substitute for Eosin in Hematoxylin-Eosin Histological Staining
Full text linkStaining is an important stage in preparing microscopic preparations so that the histological structure can be observed clearly. Hematoxylin-Eosin is the most widely used dye. Unfortunately, eosin is a synthetic chemical compound that is carcinogenic. To reduce the health and environmental impacts of eosin compounds, it is important to look for alternative dyes from natural ingredients. Hibiscus (Hibiscus rosa-sinensis L.) is a plant known to contain anthocyanins which can be used as eosin substitutes. This study aims to determine the potential of hibiscus extract as a substitute for eosin in histological staining. In this study, unstained liver tissue preparations from mice (Mus musculus) were used. Hibiscus extract dye was compared with eosin as a control. The color quality of the tissue preparations was used as an experimental parameter. The results of the microscopic assessment showed that liver tissue stained with Hibiscus extract at concentrations of 60% and 80% was less good than eosin. However, because tissue can stain reddish, hibiscus extract has the potential to be used as an eosin substitute. The right extraction method may be able to produce intact anthocyanin so that it can color the tissue optimally
Insect Species Diversity in Bangek River Area Lubuk Minturun Padang
Full text linkThe study employed the insect net method to sample insects from the Bangek River. Primary and secondary data were collected for analysis. The research was conducted at Bangek River, Lubuk Minturun District, Padang City, West Sumatra Province. Insect sampling involved a single event using an insect net within the administrative area of Lubuk Minturun. A total of 35 insects were captured from the Bangek River, comprising 5 species from the Lepidoptera order, 28 from the Odonata order, and 1 species each from the Hemiptera and Orthoptera orders
Isolation and Identification of Fungi from Bactrocera dorsalis as Candidate Entomopathogenic Fungi
Full text linkCultivation of horticultural crops especially fruits, has long been practiced in Indonesia. Citrus plants are one of the main fruit commodities developed in Indonesia. Increased citrus production is inseparable from challenges, especially in controlling harmful pest attacks. Bactrocera fruit fly has attracted worldwide attention because of its ability to attack more than a hundred horticultural crops and cause crop failure. Various ways have been done to control Bactrocera, such as protecting the fruit with wrapping and even the use of synthetic insecticides that cause many losses. To overcome this, innovations are needed using biological control. Entomopathogenic fungi are potential candidates for bioinsecticides. This study aims to isolate and identify entomopathogenic fungi from Bactrocera dorsalis from citrus plants. The entomopathogenic fungus isolation method uses the moist chamber method. Then the fungal isolates will be identified macroscopically and microscopically. The isolation results show that five types of fungi are identified from the body of Bactrocera dorsalis. The five fungi can be used as candidates for entomopathogenic fungi
Water Quality Analysis Based On Diversity and Abundance of Macrozoobenthos In Way Semah River Waters of Pesawaran
No full textRiver pollution is a major problem in the water quality crisis due to certain factors, including human activities around the river ecosystem which are strongly suspected to be the main cause of the problem. The purpose of this study was to determine the diversity and abundance of macrozoobenthos and the level of water pollution in the Way Semah Pesawaran River. The research was conducted using three indicators of water quality: biological indicators in the form of macrozoobenthos, physical indicators in the form of temperature and brightness, and chemical indicators in the form of pH, DO, and BOD. Sampling was carried out at 3 stations with two points, namely the right bank and left bank points at each station. The method of determining the location and data collection using the purposive sampling method. Based on the results of the analysis of abundance, diversity, uniformity and dominance of macrozoobenthos obtained the ecosystem of Way Semah River waters showed mild to moderate polluted conditions with a range of 1.21 - 1.8
Effectiveness of Coconut Water and Atonics on the Growth of Eggplant (Solanum melongena) Seedlings of the Laguna F1 Variety: EFEKTIVITAS PEMBERIAN AIR KELAPA DAN ATONIK TERHADAP PERTUMBUHAN KECAMBAH TERONG UNGU (Solanum melongena) VARIETAS LAGUNA F1
No full textEggplant (Solanum melongena L.) is an authentic plant native to tropical climates, widely favored by the community, and available at an affordable price. The demand for eggplant in Indonesia reached 69,963 tons in 2015 and has continued to increase to 74,081 tons. Based on FAO data in 2016, Indonesia is one of the eggplant-producing countries, ranking sixth in the world after China, India, Egypt, and Turkey. The Laguna F1 eggplant variety is known for its quality and resistance to bacteria-causing wilting. To minimize the cultivation time of eggplants, it is necessary to use readily available natural fertilizers, one of which is coconut water. The utilization of coconut water as fertilizer is due to its content of various hormones that play a crucial role in plant growth, namely auxin, cytokinin, and gibberellin. The purpose of this research is to investigate the effects of coconut water and atonic, determine the optimum concentrations, and understand the interaction between coconut water and atonic in the growth of eggplant plants. The research was conducted in the Botany Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Lampung, in October-November 2019, using a 2x3 factorial design. The first factor involved coconut water at concentration levels of 25%, and 50%, and compared with a control group (0%). The second factor was the atonic solution with a concentration of 10 ml/l, compared with a control. The treatments were repeated in five replications, resulting in 30 experimental units. Observed variables included plant height, fresh weight, dry weight, relative water content, and chlorophyll content. The results of this research can be summarized as follows: the application of coconut water and atonic has a positive effect on the growth of eggplant plants. Additionally, the combined induction of coconut water at a concentration level of 25% and atonic at 0% demonstrated superior outcomes in terms of plant height, fresh weight, dry weight, relative water content, and chlorophyll content in eggplant plants
Colchicine Effect of Mitosis Root Tip by Banana Kepok Yellow (Musa paradisiaca L.) Polyploid Explants
Full text linkColchicine can inhibit spindle formation so that doubled chromosomes fail to separate during anaphase cytologically to induce mutations that produce polyploid plants. Yellow kepok banana is a triploid banana. The supply of yellow kepok bananas is hampered by conventional cultivation methods and the limited availability of quality seeds. This study aims to determine the effect of adding colchicine compound to tissue culture media on the mitotic index, chromosome number, and chromosomal abnormalities in yellow kepok bananas and the formation of polyploid plantlets. This study was included in the experimental study by conducting mitotic preparation and observing morphology after the test sample was induced by colchicine. The results of the research that has been done show that the addition of the compound colchicine to the tissue culture medium of yellow kepok banana causes a decrease in the mitotic index, an increase in the number of chromosomes, the presence of chromosomal abnormalities, the formation of polyploid plantlets, and an increase in the number of roots, but there is a reduction in root length
Differences in Soaking Time of Sea Urchin Gonad Extract (Diadema setosum) on Nile Tilapia (Oreochromis niloticus) Masculinization with a Dose of 4 mg/L : Perbedaan Lama Perendaman Ekstrak Gonad Bulu Babi (Diadema Setosum) Terhadap Maskulinisasi Ikan Nila (Oreochromis Niloticus) Dengan Dosis 4 Mg/L
No full textNile tilapia (Oreochromis niloticus) is a fish that is in demand because of its high economic value and thick meat. Tilapia is easy to breed, uncontrolled spawning can occur so that growth is inhibited. The growth of male tilapia is faster than females at the same age. To increase the growth rate, sex reversal is carried out towards males through immersion in steroid hormones. Bioactive substances contained in sea urchins are steroid compounds. The purpose of this study is to determine the effect of the length of immersion in sea urchin gonad extract (Diadema setosum) on the formation of male phenotypes of tilapia larvae and tilapia survival. The method used is a completely randomized design with 4 treatments and each treatment with 3 replicates at a dose of 4 mg/L with soaking times of 0, 12, 18, and 24 hours. Data are analyzed using SPSS 16 software with analysis of variance (One-way ANOVA). The results show that different lengths of immersion in sea urchin steroid extract at a dose of 4 mg/L affect the formation of male individuals. A soaking time of 18 hours is quite effective in directing the sex of fish to males by 66%. Immersion time also affects the survival of tilapia larvae
Types, Prevalence, and Infection Rate of Ectoparasites on Dumbo Catfish (Clarias gariepinus): (Jenis, Prevalensi, an Tingkat Serangan Ektoparasit Pada Ikan Lele Dumbo (Clarias Gariepinus)
No full textCatfish (Clarias gariepinus) is a fish that is cultivated for human consumption. The success rate of cultivation is influenced by many factors, including the presence or absence of parasitic infections in cultivated fish. This study aims to determine the type, prevalence, and level of ectoparasite attacks on Dumbo Catfish in the Rajabasa Raya fishing pond, Bandar Lampung. The ectoparasites were taken using the method of scraping mucus and staining the ectoparasites on the surface of the fish's body, and then identified. The prevalence rate is determined using the Kabata prevalence formula. The results showed that two types of ectoparasites were found, namely Dactylogyrus sp. with the highest prevalence of 100% (infection rate is always), and Gyrodactylus sp. with the highest prevalence of 80%. (infection rate is common)
Microplastic Analysis at Sea Water and Sediment in the Mahitam Island Lampung Bay using FT-IR
Full text linkThe amount of garbage that accumulates in the waters causes environmental damage which is getting worse. The breakdown of plastic waste into smaller particles that have been degraded is called microplastic. This study aims to analyze the number of particles, shapes, and types of microplastic polymers found in the water and sediments of Mahitam Island. The research location was determined by purposive random sampling with three stations based on plastic sources, namely Station I, Station II, and Station III, which had different characteristics from laboratory-based testing for seawater and sediment. Microplastic analysis on seawater samples was carried out by adding a solution of 70% ethanol, 30% H2O2, and 30% NaCl, and on sediment samples was carried out by adding a solution of FeSO4 (0.05 M), NaCl, and 30% H2O2. The content of the number of particles and the shape of microplastics were analyzed using a microscope, while the analysis of microplastic polymers using FT-IR. The first thing to do is to extract microplastics The forms of microplastic found are Fiber, Film, Fragments, and Pellets. The highest number of microplastic particles was the type of film for water samples at Station 1 and Station 2 for sediment samples. The types of polymers found in water and sediments on Mahitam Island are polyvinyl chloride (PVC), polyethylene (PE), polypropylene (PP), and polystyrene (PS) which come from plastic waste. Microplastics are known to originate from the use of plastic originating from tourist activities and originating from the sea currents of Mahitam Island