Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)
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Determination of Aedes Spp. Mosquito Breeding Spots as a High-Risk Area Through Maya Index Analysis in Way Halim Bandar Lampung
Full text linkDengue Haemorrhagic Fever (DHF) is a disease that is transmitted through the Aedes aegypti and Aedes albopictus mosquito vectors. The Maya index (MI) is an indicator to identify whether an area is at high risk as a breeding site for mosquitoes. This study aims to determine the types of breeding places and types of mosquito larvae of DHF and MI vectors in the Way Halim District of Bandar Lampung. The method used was a survey method on 100 houses in 5 villages in Way Halim District, namely Jaga Baya 1, Jaga Baya 2, Jaga Baya 3, Way Halim Permai, and Gunung Sulah. Data analysis to determine MI uses the Breeding Risk Index (BRI) and Hygiene Risk Indicator (HRI) indicators. The results of this study indicate that there were 225 containers inside the house observed, where 51 containers of them were found to have Aedes mosquito larvae while outside the house found as many as 28 containers with 8 containers including larvae. The identification results found 2 types of Aedes mosquitoes namely, Ae. aegypti (88.24%) and Ae. albopictus (11.76%). The results of the MI analysis show that Way Halim District is classified as a District with a moderate MI category with an MI value of 46
The Utilization of Rice Wastewater Combined With Red Dragon Fruit (Hylocereus polyrhizus) as Basic Ingredients of Nata De Leri
Full text linkNata de leri is one of the products produced from fermented organic white, red, black rice washing water with the addition of red dragon fruit skin extract. In javanese the rice washing water is called Leri so that the resulting nata is called “Nata De Leri”. (carbohydrates, proteins, minerals) that are carried away from the rice membrane during the washing process. So that it can be utilized by microorganisms as a medium in making nata. The addition of red dragon fruit skin extract gives color because it contains a lot of anthocyanin and also the fiber and water content is very high. The purpose of this research was to determine the organoleptic and chemical characteristics of nata. This research used a qualitative descriptive method using a 2 factor complete randomized design and the treatment given was 75% of white, red, black rice washing water concentration, The concentration of red dragon fruit skin extract 75%, 50%, and 25%. The results showed that the most preferred texture on the addition of 75% red dragon fruit skin extract was an average of 4,65, the most preferred color in the addition of 75% and 25% red dragon fruit skin extract, the aroma of the average panelist doesn’t like all the aroma of nata de leri, for the taste all the samples were liked by the panelists because of their sweet taste. While the water content and crude fiber content produced are quite high on average but do not exceed SNI
KARAKTERISTIK HABITUS DAN LINGKUNGAN POHON SARANG SEMUT RANGRANG (Oecophylla smaragdina) DI BANDAR LAMPUNG
Full text linkThe weaver ant (Oecophylla smaragdina) is a eusocial insect, whose colonic life is highly dependent on the existence of trees. The existence of the weaver ant in natural habitat is now reduced due to the destruction of habitats caused by humans. To preserve the existence of weaver ant in nature needs to be studied bioecology as the purpose of this research is to know the characteristics of habitus and environment of weaver ants nest in Bandar Lampung. The research was conducted in June-October 2017 at the location of yard and plantation, using 50% sample from the sample population, from 20 districts in Bandar Lampung, 10 sub-districts were used as randomly selected research sites. The results showed as many as 15 types of plants used weaver ant for nesting. The most widely planted species of weaver ant is Mangifera sp. with characteristics of slippery surface habitus, sympodial branching, tree height of 3.9 to 11.5 m and an average canopy of> 65%, more nests found in yards (76%) far from urban areas, the percentage of the dominant nest toward the east , which is 41.8% of the total number of 86 nests
Organ-Protective Effect of Red Ginger Extracts in Male Mice Induced by Paraquat
Full text linkParaquat is a toxic compound that disrupts biochemical processes in the body, cell death, and multi-organ failure. The liver and lung are the target organ of paraquat toxicity. The body needs antioxidants to counteract free radicals. Red ginger (Zingiber officinale Roxb. Var rubrum) is a spice that contains antioxidants. This study was conducted to determine the effect of ethanol extract of red ginger (Zingiber officinale Roxb. Var rubrum) on liver and lung histology male mice induced by paraquat. The research used completely randomized design and use of 6 treatment groups, i.e. control group (given distilled water and standard feed), PQ group (induced by paraquat 20 mg/kg per BW), group P1 (given paraquat and ethanol extracts of red ginger 200 mg/kg per BW), group P2 (given paraquat and ethanol extract of red ginger 400 mg/kg per BW), group P3 (given paraquat and ethanol extract of red ginger 600 mg/kg per BW), group P4 (given ethanol extract of red ginger extract 600 mg/kg per BW). The data obtained will be tested using One Way ANOVA and continued by Least Significant Difference (LSD) with a 95% confidence level. The results showed ethanol extract of red ginger effective against liver and lung histology male mice induced by paraquat. The results also showed ethanol extract of red ginger with a dose of 400 mg/kg per BW is effective in protecting the liver and lung from damage caused induced by paraquat
Effect of Drought-Stress Conditions in Chlorophyll Content of Dendrobium sp. Planlets
Full text linkDendrobium sp. was a popular ornamental plant and has high economic value. One of many difficulties in the growth of orchids in Indonesia is low humidity and lack of water availability. Regulatory Substance Growth in the form of an atonic solution can increase the growth of orchid plants. The purpose of this study was to determine the effect of atonic solutions on orchid chlorophyll content in drought stress conditions. This study used a 3x3 factorial design. Factor A is the atonic solution with 3 levels of concentration: 0 mL/L, 2 mL/L, and 3 mL/L. Factor B is PEG 6000 with 3 concentration levels: 0% b/v, 20% b/v and 25% b/v. The parameters tested were chlorophyll a, b and total Dendrobium orchid plantlets. The results showed that atonic solution and PEG 6000 significantly affected the chlorophyll a, b and total content of chlorophyll. The higher concentration of the atonic solution, the more content of chlorophyll a, b and total plantlets of decreased in drought stress conditions in vitro
Local Knowledge of Traditional Physician of Medicinal Plants
Full text linkTraditional medicine in modern era has not been completely abandoned. Now, Traditional medicine is being used again as a substitute for the use of chemical drugs. West Baturaja, Ogan Komering Ulu, located in South Sumatra is an area in Indonesia that still uses traditional medicine. This research is conducted to study the knowledge of Traditional Physician in using medicinal plants for treatment in West Baturaja, Ogan Komering Ulu, South Sumatra. This research was conducted in 5 villages in West Baturaja. We interviewed 8 Traditional Physician from 5 villages, i.e. Talang Jawa Village (4 people), Air Gading (1 people), Saung Naga (1 people), Batu Putih (1 people), and Pusar village (1 people). The Result obtained that there are 140 types of medicinal plants from 60 families. The most widely used is the Zingiberaceae. The most widely used plant habitus is a bush. The most widely used part is the leaves. The way of processing is boiled then taken by the patient. The most commonly treated disease is diabetes
Perbedaan Pertumbuhan dan Kandungan Gizi Tetraselmis sp. Isolat dari Lampung Mangrove Center pada Kultur Skala Semi Massal dengan Konsentrasi TSP Berbeda
Full text linkProteolytic Activity of The Entomopathogenic Fungi (Penicillium sp. ) of Cockroaches (Periplaneta americana)
Full text linkCockroaches (Periplaneta americana) were the insect vectors of disease that caused adverse effects on human health. Control cockroch excessive use of insecticides can lead to residus in the environment and resistance cockroach. Therefore it was necessary to use an alternatives such as such as entomopathogenic fungal as biologycal agents. The entomopathogenic fungi penetrated via the integument of an cockroach to reach the hemocoel. Proteins were the molecules responsible for integument strength in cockroach, It was synthesis the proteases to degrading proteins. The study begins with the isolation of entomopathogenic fungi using the moist chamber method with cockroach as insect bait. Fungus that grow on cockroches are cultured and purifed on Potato Dextrose Agar (PDA) medium then identified. Identification was carried out through macroscopic observations including colony color and diameter and microscopic observations including conidia, conidiophores, hyphae, vesicles, fialids, and leg cells. The result of isolation and identification obtained as Penicillium sp. Proteases enzimatic activity tested on PDA with anlene 1%. The clear zone formed is measured to show the activity of proteases produced by Penicillium sp
PENGARUH ASAM GIBERELAT (GA3) DAN KALIUM NITRAT (KNO3) SERTA INTERAKSINYA TERHADAP SENESCENCE BUNGA POTONG GERBERA PUTIH (Gerbera jamesonii).
Full text linkThe purpose of this study was to decide whether the mixture of KNO3 and GA3 solutions was more effective than a single solution of KNO3 or GA3 in maintaining the freshness of cut flowers. The study was conducted at the Botanical Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung from November - December 2017. The research was carried out in a 2 x 3 factorial experiment with reason A was the GA3 solution with 3 concentration levels: 0%, 0.5%, and 1%. Factor B is KNO3 solution with 2 concentration levels: 0% and 5%. The limits in this study were fresh weight, dry weight, relative water content, and total dissolved carbohydrate content of gerbera cut flowers, qualitative limits were the level of reducing sugars. Measurements were made 7 days after soaking cut flowers in KNO3 or GA3 solutions, and a mixture of both. Homogeneity of variance and analysis of variance were determined at a significant level of 5%. Simple effect of KNO3 at each concentration level of GA3 decide by F test at 5% significance level. The results showed that GA3 with a concentration of 0.25% and 0.5% increased the fresh weight and dry weight of Gerbera cut flowers. KNO3 0.5% much reduced the relative water content of Gerbera cut flowers. The interaction between GA3 and KNO3 does not much effect all freshness of cut flowers. From the results of the study concluded that the single treatment of GA3 was more effective than the combination treatment of GA3 and KNO3 in maintaining the freshness of Gerbera cut flowers
PENGARUH ARANG AKTIF, BENZILADENIN, DAN KINETIN TERHADAP PERTUMBUHAN TUNAS JATI SOLOMON (Tectona grandis Linn. f) IN VITRO
Full text linkTissue culture techniques can be used for propagation of uniformaly large teak solomon seeds.The purpose of this research was knowing the effect of activated charcoal, the addition of benzyladenine (BA) and combination of BA with 6-furfurylaminopurine (kinetin) to the growth of shoots of solomon teak in vitro. The solomon teak explants used were singlestem cuttings from aseptic shoots obtained from in vitro cultures. This research was conducted in laboratory with complete randomized design with 3 replications. The experimental treatment was a single factor consisting of basic MS medium (Murashige and Skoog, 1962), with 6 treatments: MS without growth regulator (control), MS without growth regulator + 2 g/l activated charcoal, MS + 0,1 m/l BA , MS + 0,2 m/l BA, MS + 0,1 m/l BA + 0,1 m/l kinetin and MS + 0,2 m/l BA + 0,1 m/l kinetin. Observation on the number of books/ shoots, number of leaves/ shoots, shoot/ bud height and visual apperance of culture was taken at 8 weeks after planting. The data were analyzed for variety and continue the separation of the LSD at 5% level. The results showed that in general, all six treatments could be used for propagation of in vitro teak solomon (Tectona grandis Linn. f) and produced at least 6,22 books/ shoots every 8 weeks. The best media were MS medium + 0,1 m/l BA and MS + 0,1 m/1 BA + 0,1 m/l kinetin, because it able to produce 7,78 books/ shoots. The highest number of leaves was obtained at the treatment of MS + 0,1 m/l BA, while the average shoots/ shoots produced were not different for all