GreenPrints Institutional repository of De La Salle Medical and Health Sciences Institut
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    6526 research outputs found

    Artwork 069: Josefa, Teodora, Gabriella

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    Artwork 088: Shopaholic Venus

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    Antibacterial activity of silver nanoparticles of rambutan leaves (Nephelium lappaceum) against Pseudomonas aeruginosa

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    The researchers aim to determine the bioactive compounds of the crude rambutan leaves extract, identify the presence of silver nanoparticles using Ultraviolet-Visible (UV-Vis) Spectroscopy, Fourier Transform Infrared (FTIR) Spectroscopy and Scanning Electron Microscope (SEM) and Energy Dispersive X-ray Spectroscopy (EDX), and to determine the Minimum Inhibitory Concentration (MIC) of green synthesized silver nanoparticles from Rambutan leaves extract against Pseudomonas aeruginosa, an aerobic gram-negative bacterium which can cause nosocomial infections associated with high morbidity and mortality rates in immunocompromised patients. The synthesized rambutan leaves extract was characterized using UV-Vis Spectroscopy, FTIR Spectroscopy and SEM. The results from UV-Vis Spectroscopy showed peaks for the synthesized silver nanoparticles with absorbance values of 0.071, 0.045, 0.044, and 0.043 at wavelengths of 340, 436, 446, and 460 nm, respectively. Meanwhile, FTIR Spectroscopy showed peaks of 3,400; 3,200; 2,260, 1,680; and 1,350 cm−1 as it is associated with carboxyl (-C=O) and (-NH) amine groups which acts as a reducing and stabilizing agent in AgNPs synthesis. SEM images showed agglomerated rod-shaped and spherical structures of the silver nanoparticles. The results obtained from Wells A, B, & C shows that the green synthesized silver nanoparticles of the rambutan leaves extract have potential antibacterial activity against P. aeruginosa as Wells A & B at a concentration of 0.5 mg/mL and Well C at 1 mg/mL

    Neutralizing property of Limnophila aromatica (rice paddy herb) and Momordica charantia (bitter gourd) against Naja philippinensis (philippine cobra) venom

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    This study investigates the venom-neutralizing potential of crude extracts from two indigenous medicinal plants, Limnophila aromatica (Rice Paddy Herb) and Momordica charantia (Bitter Gourd), against the Naja philippinensis (Philippine cobra). The research identified the predominant bioactive compounds—particularly phenolics and flavonoids—present in the leaves of these plants and determined the optimal extract concentration required for effective venom neutralization. Using an indirect hemolytic assay, the study quantified the inhibition of phospholipase A₂ (PLA₂) activity, a key enzyme in cobra venom toxicity, and compared the efficacy of the plant extracts against the Purified Cobra Antivenom (PCAV) of equine origin. The results demonstrated significant venom-neutralizing activity, with both extracts exhibiting optimal inhibition at a concentration of 100 µg/µL; specifically, Momordica charantia reduced the hemolytic halo to approximately 14.9 mm and Limnophila aromatica to about 15.1 mm—values that, while slightly higher than the positive control (12.1 mm), are comparable to conventional antivenom treatments. These findings highlight the potential of locally available medicinal plants as alternative therapeutic agents for snakebite management, particularly in regions with limited access to commercial antivenoms. The study underscores the need to explore further plant-based antivenom therapies to develop cost-effective and sustainable solutions for cobra envenomation

    Artwork 042: Why We Love Who We Love

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    Artwork 047: Purple Bloom

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